• Title, Summary, Keyword: germination

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Effect of Sowing Time on Germination and Early Seedling Growth of Quercus floribunda Lindl.

  • Karki, Himani;Bargali, Kiran;Bargali, SS
    • Journal of Forest and Environmental Science
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    • v.34 no.3
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    • pp.199-208
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    • 2018
  • Quercus floribunda (Tilonj oak) is among the five species of Quercus and an evergreen tree found in Kumaun Himalayan region. Timing of germination is a crucial event determining the success of seedling establishment and survival. The aims of the study were to investigate the effect of sowing date on the germination and morphological responses of Tilonj oak (Quercus floribunda), cultivated during the month of August-September, with supplementary irrigation. The experiment was conducted by sowing seeds at two dates with one month interval in glass house conditions at DSB Campus, Nainital, Uttarakhand, India. Present study revealed that higher germination percentage (46.67 %) was recorded at sowing time $S_2$ as compared to the sowing time $S_1$ (32.86 %). Germination percentage as well as seedling growth were affected by sowing date though the differences were insignificant. At both the sowing dates, highest germination percentage was recorded for large seeds (32.86-46.67 %) followed by medium (31.43-33.33 %) and lowest germination (6.67-7.14 %) was recorded for small size seeds. In all parameters assessed, sowing time $S_2$ had the best performance in combination with large seed size. Thus, September is suitable month for best germination, growth and seedling vigour and large seed size is recommended to silviculturists and tree planters. The information on seed germination and seedling growth is vital both for conservation and rehabilitation of degraded lands.

Studies on the Effects of Some Chemicals on the Germinative Stimulatilon of Clostridiu chauvoei Spore (우기종저균(牛氣腫疽菌) 아포(芽胞)에 대(對)한 몇가지 화학제(化學劑)의 발아촉진(發芽促進) 시험(試驗))

  • Seo, Boo Kap
    • Korean Journal of Veterinary Research
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    • v.9 no.2
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    • pp.19-25
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    • 1969
  • The effects of some chemicals for the germinative stimulation of Clostridium chauvoei spore studied. The results obtained were as follows: 1. Cooked meat medium (CMM) was superior to Liver-liver bouillon (LLB) for cultivation of the organisms. 2. Heating the organisms at $70^{\circ}C$ for 30 minutes prior to cultivation in CMM stimulated the germination rate. 3. The addition of 0.5mM L-alanine to CMM was found to be most effective for the rapid germination of the spores. 4. The addition of 0.2 mM D-alanine to CMM inhibited the germination of the spores even if the spores were heat treated. 5. The addition of 0.1mM manganese retarded the germination time, but the final germination rate was greater with 0.1mM manganese than with 0.5mM L-alanine. 6. The addition of both 0.5mM L-alanine and 0.1mM manganese stimulated the germination rate to 100 per cent. 7. The germination rate was greater with the addition of germination stimulants than without any stimulants. The germination rate was approximately 6 per cent greater with prior heat treatment than without heat treatment. 8. The optimum time for the harvest of vegetative forms of the organisms was 15 hours after cultivation in the media which contain suitable germination stimulants.

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Effect of Pre-sowing Treatments on Seed Germination and Seedling Growth of Canarium resiniferum, A Rare Native Tree of Bangladesh

  • Hasnat, G.N. Tanjina;Hossain, Mohammed Kamal;Alam, Mohammed Shafiul;Hossain, Md. Akhter
    • Journal of Forest and Environmental Science
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    • v.33 no.3
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    • pp.226-232
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    • 2017
  • Dhup (Canarium resiniferum) is an economically and aesthetically important rare native tree species of Bangladesh. In natural condition 78.5-98.7% seeds do not germinate due to inhibition by hard seed coat, seed predation and unfavorable environmental conditions. A study was conducted in the Seed Research Laboratory and nursery of Institute of Forestry and Environmental Sciences, Chittagong University, Bangladesh in 2013 to find out appropriate pre-sowing treatments for maximizing germination and initial seedling growth. Eleven pre-sowing treatments were provided in both the seeds sown in polybags and seeds sown in propagator house. Results revealed that, germination started at first (after 20 days of seed sown) in seeds immersed in water at room temperature for 24 hours and germination completed within 38 days. Significantly higher (p<0.05) germination percentage (33%), germination energy (16.7%), plant percent (33%) and germination value (0.4) was found with seeds immersed in water at room temperature for 24 hours. Seedling height measured at three, four and five months after the seed germination in case of seeds treated by immersion in water for 24 hours was also greater than others. Therefore, pre-sowing treatment by immersion in water at room temperature for 24 hours was more effective in germination and production of quality vigor seedlings of Dhup.

Optimization of in vitro seed germination of Taraxacum platycarpum

  • Lee, Jung-Hwan;Kim, Young-Kwan;Oh, Eun-Yi;Jung, Kuk-Young;Ko, Ki-Sung
    • Korean Journal of Environmental Agriculture
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    • v.28 no.4
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    • pp.403-408
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    • 2009
  • Dandelion (Taraxacum platycarpum) has been widely utilized for medicinal purposes. However, the dandelion seeds are relatively difficult to germinate under cultivation conditions, which hampers seedling propagation of dandelion plants and reduces the opportunity of usage of such a useful medicinal plant. Thus, in this study, in vitro conditions for the dandelion seed germination were optimized to enhance the germination rate. In seed washing steps, the sequential treatments with 20% of ethanol, 20% of NaOCl, and distilled water avoided microbial contamination with the highest in vitro germination rate (67.5%) from seeds sown in germination media. The media supplemented with 1.4 g/L of MS salts and 1% of sucrose significantly enhanced the germination rate compared to the media with 4.4 g/L of MS and 3% of sucrose. Sowing the seeds vertically in the optimized media supplement conditions, 1.4 g/L of MS salts and 1% of sucrose, gave the maximum in vitro germination rate (61%), which was almost three times higher than sowing seeds on a soil pot (23%). Our results indicate that the seed washing and sowing methods including germination medium supplements can be optimized to enhance in vitro seed germination of dandelion.

Effects of Germination on Fatty Acid and Free Amino Acid Profiles of Brown Rice 'Keunnun'

  • Choi, Induck;Suh, Sae-Jung;Kim, Jae-Hyun;Kim, Sun-Lim
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.799-802
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    • 2009
  • The effect of germination on hydration and germination properties, and on the changes of fatty acids and amino acids profiles of a brown rice 'Keunnun' (KN) with a large embryo was compared to 'Ilpumbyeo' (IP) with a normal embryo. A rapid germination up to 24 hr was observed in both brown rice cultivars, afterward decreased with germination time. At 60 hr, the KN ($86.0{\pm}4.24%$) showed slightly lower germination capability than the IP ($97.0{\pm}1.41%$). Lower water uptake during germination was also found in the KN ($1.22{\pm}0.02\;g$) compared to the IP ($1.59{\pm}0.05\;g$). Major fatty acids were palmitic acid, oleic acid, and linoleic acid accounting for more than 95% of total fatty acids. The most abundant amino acid in both types was oleic acid, which was decreased during germination, whereas palmitic and linoleic acids were increased. Eight amino acids were detected, and a remarkable increase in ${\gamma}-amino$ butyric acid (GABA) during germination was observed. The KN was characterized with higher tasty amino acids of aspartic acid, glutamic acid, glycine, and alanine.

Changes of Protein and Lipid Composition During Germination of Perilla frutescens Seeds (들깨 종자의 발아에 따른 단백질 및 지방의 조성 변화)

  • 정대수;김현경
    • Journal of Life Science
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    • v.8 no.3
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    • pp.318-325
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    • 1998
  • To investigate changes in protein and total lipid contents, seed storage protein pattern, and fatty acid composition of germination perilla(Perilla frutescens) seeds. Also, the corresponding value components in cotyledons, hypocotyles and roots were measured according to germination stage. The results were summarized as follows ; During germination, pertein and total lipid contents of Yepsilldalggae and Kwangyang cultivar were decreased continuously. In particular, protein contents rapidly decreased to the 3 days after germination(DAG), and then total lipid contents rapidly decreased between 3 DAG and 10 DAG. In changes of protein and total lipid contents of cotyledons, protein contents of Yeupsildalggae was increased during the germination, but Kwangyang cultivar was decreased during the same periods. The total lipids contents of Yeupsildalggae and Kwangyang cultivar were decreased during the germination. According to SDS-PAGE analysis, there was no detectible polypeptide bands on the gel before seed germination suggesting that this may be due to the rapid degradation of the storage proteins in the mature seed by hydrolyttic enzymes during the stage. During germinatation , the polypeptide band with 27$\sim$28KD of Yeupsildalggae and Kwangyang cultivar were accumulated gradually. In changes of fatty acid composition of total lipid of Yeupsildalggae and Kwangyang cultivar , saturated fatty acids such as palmitic acid and stearic acid increased during the germination. On the other hand, unsaturated fatty acid such as linoleic acid and linolenic acid decreased during the same periods. However, oleic acid increased to the 5 DAG, and then was repidly decreased.

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Germination Enhancer and Wetting Agent for Quick Establishment of Kentucky bluegrass Cultivars

  • Lee, Sang-Kook
    • Weed & Turfgrass Science
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    • v.6 no.4
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    • pp.313-321
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    • 2017
  • Wetting agent is designed to reduce the surface tension of the liquid and spread more easily across or penetrate into the soil against water repellency. The effect of wetting agent to seed germination is not clear. Using germination enhancer is one of the methods to increase the germination speed of turfgrass seeds and to shorten establishment period. The objective of the study was to evaluate germination enhancer and wetting agent for quick establishment of various Kentucky bluegrass cultivars. The germination enhancer was used at two levels of 0.3 and $0.6ml\;kg^{-1}$ as low and high, respectively. Two levels of wetting agent were of 0.46, and $0.92ml\;m^{-2}$ as low and high, respectively. Germination enhancer has no synergistic effect with wetting agent. When quick establishment is required, selection of cultivar would be more effective instead of using germination enhancer and wetting agent. Among Kentucky bluegrass cultivars, 'Award' had the greatest turfgrass coverage for establishment and the greatest turfgrass color and quality based on the result of the study. When quick establishment is required, selection of cultivar would be more effective instead of using germination enhancer and wetting agent.

Effects of Growth Regulators on the Germination of Panax ginseng C.A. Meyer (인삼종자의 발아에 미치는 식물생장조절물질의 영향)

  • 권우생;정찬문
    • Journal of Ginseng Research
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    • v.10 no.2
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    • pp.159-166
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    • 1986
  • Effects of plant growth regulators on the germination of ginseng (Panax ginseng C.A. Meyer) seeds were investigated. Ginseng seeds germinated more vigorously in the treatments of kinetin and BA, and the promoting effect of kinetic on the germination and the growth of rootlet enhanced in low temperature ($10^{\circ}C$). However, GA did not promote the germination of dehiscent seed. The optimum temperature for germination of dehiscent seed was $10^{\circ}C$ and the range of effective concentration of kinetin for germination was 50 to 100 ppm.

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Priming Effects on Germination of Aged Tobacco Seeds

  • Min, Tai-Gi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.46 no.4
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    • pp.325-327
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    • 2001
  • Tobacco (Nicotiana tabacum L. cv KF109) seeds were artificially aged in a controlled environment of 45$^{\circ}C$ and 80% relative humidity condition for different duration up to 14 days before priming in polyethylene glycol 6000 solution of equivalent osmotic potential of -0.8 MPa for 8 days at $25^{\circ}C$. The seeds aged only and primed after aging were germinated at 15$^{\circ}C$ and $25^{\circ}C$ to observe the priming effects on the germination of aged seeds at different temperature. The germination percentage of the aged seeds was rapidly dropped starting from 8 days of aging and mean germination time (T$_{50}$) was greatly increased, particularly in germination at 15$^{\circ}C$. The germination capacity was greatly restored in the primed seeds after aging, particularly in the seeds of longer aging and germinating at 15$^{\circ}C$.>.

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Study on the Seed Germination and Salt Tolerance of Plants in Reclaimed Salt Area (간척지내 식물종자의 내염성과 발아에 관한 연구)

  • 김철수
    • Journal of Plant Biology
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    • v.23 no.1
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    • pp.27-33
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    • 1980
  • The germination character and the salt tolerance of seeds collected in a salt reclaimed area were studied. Twenty species of seeds out of 30 species collected were germinated under natural or continuous light conditions but only 16 species under dark condition. Germination percentage of seeds under dark was more decreased than those under other two conditions and speed of germination was accelerated at high temperature. It is clear that almost all the wild weeds were the light germinated seeds. The higher concentration of NaCl for germination inhibited to make the lower percentage and rate of germination. The germination curves of seeds treated with NaCl solution were classified into 3 different curves; a steeper, a parabola and intermediate. The critical concentrations of NaCl for germination were 2.3% for Brassica napus, 1.8% for Echinochloa hispidula, 1.5% for Setaria lutescens, 1.3% for Aster koraiensis, 0.7% for Bromus japonicus and 0.6% for Glyceria acutiflora.

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