• Title, Summary, Keyword: glucokinase

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Cordyceps militaris Increases Hepatic Glucokinase Activities (Cordyceps miiitaris에 함유된 혈당강하 성분이 간세포 Glucokinase활성에 미치는 영향)

  • Kim, Hyun-Sook;Roh, Young-Joo;Choe, Myeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.2
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    • pp.158-161
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    • 2005
  • We have isolated fractions from Cordyceps militaris and Paecilomyces japonica and investigated their effects on the activity of rat liver cytosolic glucokinase, a key metabolic enzyme involved in carbohydrate metabolism. The dried powder of the C. militaris and P. japonica were successively extracted with ethanol and with 70% ethanol. The residue was exhaustively extracted with hot water. The extract was dialyzed against water, and to the non-dialyzable solution was added 2 volumes of ethanol. The precipitate was collected by centrifugation dispered in water, and lyophilized to afford fraction A. The residue after hot-water extraction was suspended in 5% sodium carbonate. The final residue was suspended in 5% NaOH. The alkaline suspension was purified in a similar manner as described above to afford fraction B. Hepatic glucokinase activities of the fraction A extracted from C. militaris and P. japonica were 371.4 and 379%, respectively. The fraction B was 314.2 and 147.4%. The activity of fraction B of C. militaris extracts was higher than that of P. japonica. Liver cytosolic glucokinase activity of rats fed normal diet supplemented with 0.1% C. militaris was 1316%. In conclusion, the present study has demonstrated that C. militaris extracts were able to prevent sudden postprandial peaks in blood glucose as a result of a marked increase in the liver cytosolic glucokinase activities.

Effect of Rhois Galla extract on pancreatic glucokinase and hexokinase activities of interleukin-1β-induced diabetes mellitus mice (Cytokine interleukin-1β로 유발(誘發)된 당뇨병(糖尿病) 마우스의 췌장(膵臟) glucokinase 및 hexokinase 활성(活性)에 대(對)한 오배자(五倍子)의 효과(效果)에 관(關)한 연구(硏究))

  • Choi, Hyung-Il;Jeong, Ji-Cheon;Kim, Cheorl-Ho
    • The Journal of Dong Guk Oriental Medicine
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    • v.7 no.1
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    • pp.65-74
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    • 1998
  • We investigated the in vivo effect of an aquous extract from Rhois Galla (R-G) on glucokinase and hexokinase activities of diabetes mellitus induced by interleukin-$1{\beta}$ ($IL-1{\beta}$). After 1 week of alloxan injection, the levels of serum glucose and insulin secretion were dramatically increased, however, the insulin secretion was decreased with administration of R-G. $IL-1{\beta}$ injection allowed the serum glucose level increased and the level was decreased by R-G administration. Furthermore, we could observe that R-G was effective in recovering the levels of insulin secretion. Enzyme activities of the glucokinase and hexokinase were decreased by $IL-1{\beta}$ treatment In contrast, R-G administration to the mice allowed proportion increasing. Seemingly, when $IL-1{\beta}$ was injected to the mice, enzyme activities of the glucokinase and hexokinase were decreased. But, R-G stimulated induction of enzyme activities of the glucokinase and hexokinase as high as normal group. These results suggested that R-G is highly effective in treatment of diabetes mellitus.

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Effect of Sea Tangle, Laminaria japonicus, Extract on The Activities of Glucokinase and Hexokinase in Alloxan-Induced Diabetic Mellitus Mice (Alloxan 처리 당뇨병 마우스의 췌장 glucokinase 및 hexokinase에 대한 다시마 열수추출물의 효과)

  • 김동수;김철호
    • Journal of Life Science
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    • v.11 no.5
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    • pp.476-482
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    • 2001
  • The study was undertaken to determine the effect of the aqeuous extract of sea tangle, Laminaria japonicus, on the activites of glucokinase and hexokinase in the pancreas of diabetic mellitus mice which were induced by alloxan. After one week of alloxan injection, the levels of serum glucose and insulin secretion were dramatically increased, however, the insulin secretion was decreased with administration of sea tangle extract. Alloxan injection allowed the serum glucose level to increase and level was decreased by sea tangle extract administration. Furthermore, it was observed the sea tangle extract was effective in recovering the levels of insulin secretion. Enzyme activities of the glucokinase and hexokinase were decreased by alloxan treatment. In contrast, sea tangle extract administration to the mice allowed to increase proportionally. These results suggested that sea tangle extract is highly effective in treatment of diabetic mellitus induced by alloxan.

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Viral Hemorrhagic Septicemia Virus NV Gene Decreases Glycolytic Enzyme Gene Transcription (바이러스성 출혈성 패혈증 바이러스 NV 단백질에 의한 glucokinase 전사 활성의 억제)

  • Cho, Mi Young;Hwang, Jee Youn;Ji, Bo Young;Park, Myoung Ae;Seong, Mi So;Kim, So Young;Jung, Ye Eun;Cheong, Jae Hun;Choi, Yung Hyun
    • Journal of Life Science
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    • v.26 no.12
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    • pp.1470-1476
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    • 2016
  • The viral hemorrhagic septicemia virus (VHSV), which belongs to the Novirhabdovirus genus of the Rhabdoviridae family, is a viral pathogen that causes severe losses in the olive flounder farming industry. Among six encoding VHSV proteins, the non-virion (NV) protein has been shown to have an impact on virulence. In our previous studies, transcriptomics microarray analysis by using VHSV-infected olive flounder showed that VHSV infection significantly down-regulated the mRNA expression of glycolytic enzymes. In addition, VHSV NV protein variants decreased the intracellular ATP level. Based on these results, we have tried to examine the effect of VHSV NV protein on glycolytic enzyme glucokinase expression, which phosphorylates glucose to glucose 6-phosphate. Our results indicated that the NV protein significantly decreased the mRNA expression of glucokinase in olive flounder HINAE cells. Furthermore, the NV protein played a negative role in the promoter activation of glucokinase. Furthermore, glucose uptake was effectively inhibited by VHSV infection and NV protein expression in olive flounder HINAE cells. These results suggest that the VHSV NV protein negatively regulates glycolytic enzyme expression by a transcription level and eventually leads to gradual morbidity of olive flounder through cellular energy deprivation. The present results may be useful for the prevention and diagnosis of VHSV infection in olive flounder.

Effect of Eriobotryae folium extract on glucokinase and hexokinase activities of diabetes mellitus mice induced by interleukin-$1{\beta}$ (IL-1${\beta}$ 처리(處理) 당뇨병(糖尿病) 마우스의 췌장(膵臟) glucokinase 및 hexokinase 활성(活性)에 대(對)한 비파엽(枇杷葉)의 영향(影響))

  • Yoon, Cheol-Ho;Shin, Hyeon-Chul;Jeong, Ji-Cheon
    • The Journal of Internal Korean Medicine
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    • v.19 no.1
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    • pp.466-476
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    • 1998
  • We investigated the in vivo effect of an aqueous extract (referred to as EF) from Eriobotryae folium on glucokinase and hexokinase activities of diabetes mellitus induced by $interleukin-1{\beta}\;(IL-1{\beta})$. After 1 week of $IL-1{\beta}$ injection, the levels of serum glucose concentration and insulin secretion were dramatically increased. However, the insulin secretion was decreased with administration of EF. The level of glucose concentration was decreased by EF administration. Furthermore, it was observed that EF was effective in recovering the levels of insulin secretion. Enzyme activities of the glucokinase and hexokinase, which are key enzymes of glucose phosphorylastion, were decreased by $IL-1{\beta}$. EF administration to the mice allowed proportional increasing by stimulation of induction of enzyme activities as high as normal group. These results suggested that EF is highly effective in treatment of diabetes mellitus induce by $IL-1{\beta}$.

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Alloxan처리 당뇨병 마우스의 췌장 glucokinase 및 hexokinase에 대한 다시마 열수추출물의 효과

  • 김동수;김철호
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • pp.114-115
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    • 2001
  • Alloxan은 glucokinase효소가 기질인 glucose결합부위인 -SH기를 경쟁적으로 저해함으로서 glucose의 초기 인산화를 억제하여 당뇨병을 유도시키는 화학물질이다. 본 연구에서는 이제까지의 조직학적이고 혈청학적인 연구에서 한 단계 나아가 효소학적인 측면에서 당대사와 인슐린 분비 조절인자로 알려진 glucokinase와 혈중 당을 조직내로 흡수하여 인산화시키는 효소인 hexokinase에 대한 다시마 열수추출물의 효과를 분석 검토하였다. (중략)

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Effect of Ginseng Components (Ginsenosides and Fat Soluble Fraction) on Rat Liver Glucokinase Activity (쥐의 간 Glucokinase 활성에 미치는 인삼 성분의 영향)

  • 주충노;김선진
    • Journal of Ginseng Research
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    • v.18 no.1
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    • pp.17-24
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    • 1994
  • Effect of ginsenoside mixture, ginsenoside $Rb_1$,$Rb_2$,$Rg_1$ and the fat soluble fraction of the roots of Panax ginseng C.A. Meyer on the activity of glucokinase (GK) in vitro has been observed and found that GK activity was increased about 15c1c at the concentration of ginsenoside mixture and/or the fat soluble fraction being $10^{-7}$,$10^{-5}$%. It was also observed that glucose uptake by rat liver was increased in the presence of either ginsenoside mixture or the fat soluble fraction by perfusion technique. Ginsenoside mixture stimulated various enzymes related to glucose metabolism, however, both ginsenoside mixture and the fat soluble fraction did not stimulate GK activity as expected. Primary culture of liver cells showed that the ginsenoside mixture and the fat soluble fraction increased GK activity significantly and they stimulated the GK activity synergistically in the co-presence of insulin.

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Effect of Eriobotryae folium extract on glucokinase and hexokinase activities of alloxan-induced diabetes mellitus mice (Alloxan 처리(處理) 당뇨병(糖尿病) 마우스의 췌장(膵臟) glucokinase 및 hexokinase에 대(對)한 비파엽(枇杷葉)의 효과(效果)에 관(關)한 연구(硏究))

  • Jeong, Chang-Hwan;Yoon, Cheol-Ho;Jeong, Ji-Cheon;Kim, Cheorl-Ho
    • The Journal of Dong Guk Oriental Medicine
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    • v.6 no.1
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    • pp.151-161
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    • 1997
  • We have investigated the in vivo. effect of an aqeuous extract from Eriobotryae folium on glucokinase and hexokinase activities of diabetes mellitus induced by alloxan. After 1 week of alloxan injection, the levels of serum glucose and insulin secretion were dramatically increased, however, the insulin secretion was decreased with administration of Eriobotryae folium. Alloxan injection allowed the serum glucose level increased and the level was decreased by Eriobotryae folium administration. Furthermore, it was observed that Eriobotryae folium was effective in recovering the levels of insulin secretion. Enzyme activities of the glucokinase and hexokinase were decreased by alloxan treatment. In contrast, Eriobotryae folium administration to the mice allowed proportional increasing. These results suggested that Eriobotryae folium is highly effective in treatment of diabetes mellitus induced by alloxan.

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Effects of Dietary Fat to Carbohydrate Ratio on Hepatic Glucokinase Activity in Rats (식이 중 지방과 탄수화물 비율이 쥐 간이 Glucokinase의 활성도에 미치는 영향)

  • 안현숙
    • Journal of Nutrition and Health
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    • v.25 no.2
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    • pp.109-115
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    • 1992
  • This study was undertaken to determine the effect of dietary fat to carbohydrate ratio on plasma glucose. free fatty acid level and hepatic glucokinase activity in normal or insulin treated diabetic rats. Sprague-Dawley male rats were fed with 3 different but isocaloric diets for 5 weeks. Diet 1 made to have low fat(4% corn oil and 65.8% corn starch wt/wt) : diet 2 medium fat (12% : 47.8%) : diet 3 high fat (20% : 29.8%) In the normal rats an apparent increase of GK activity was observed from the animal fed low fat diet when compared with other groups. GK activities were decreased in all the alloxan-diabetic rats than the normal rats. When insulin was injected the GK activities in all the livers of alloxan-diabetic rats restored to normal level and GK activity was highest in low fat group. In the entire group significant relationships were seen between the plasma glucose and GK activities(r=-0.6, p<0.001) FFA levels and GK activities(r=-0.63 p<0.001) Both in normal and insulin treated diabetic rats significantly depressed level of hepatic GK activity was observed in the livers of animals fed high fat diet for 5 weeks and depressed level of GK activity may be related to insulin resistance.

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Characterization of D-Glucose ${\alpha}$-1-Phosphate Uridylyltransferase (VldB) and Glucokinase (VIdC) Involved in Validamycin Biosynthesis of Streptomyces hygroscopicus var. limoneus KCCM 11405

  • Seo Myung-Ji;Im Eun-Mi;Singh Deepak;Rajkarnikar Arishma;Kwon Hyung-Jin;Hyun Chang-Gu;Suh Joo-Won;Pyun Yu-Ryang;Kim Soon-Ok
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1311-1315
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    • 2006
  • Aminocyclitol antibiotic validamycin A, a prime control agent for sheath blight disease of rice plants, is biosynthesized by Streptomyces hygroscopicus var. limoneus. Within the validamycin biosynthetic gene cluster, vldBC forms an operon of vldABC with vidA, the gene encoding 2-epi-5-epi-valiolone synthase. Biochemical studies, employing the recombinant proteins from Escherichia coli, established VldB and VldC as D-glucose $\alpha$-1-phosphate uridylyltransferase and glucokinase, respectively. This finding substantiates that the validamycin biosynthetic gene cluster harbors genes encoding the enzymes for UDP-glucose formation from glucose. Therefore, we propose that validamycin biosynthesis employs its own catalysts to generate UDP-glucose, but not depending on the primary metabolism.