• Title, Summary, Keyword: glucosinolate

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배추 및 무의 Glucosinolate 분석과 가공처리중 항암물질의 형성

  • 강갑석
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • pp.12-13
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    • 1993
  • 십자화과 채소에는 여러종류의 glucosinolate가 존재하며 이들 중 특히 indolw형 glucosinolate는 여러가지 요인에 의해 분해되어 항암효과를 나타내는 물질들을 생성한다고 보고 되고 있다. 이에 다라 우리나라 농가에서 대량 생산되고 있는 십자화과 채소인 배추 및 무에서 항암전구물질인 indole glucosinolate를 포함한 glucosinolate를 분석 및 동정하고 가공처리조건에 따라 항암물질인 indoleacetonitrile과 indolemethanol의 함량을 측정하였다. 1. GC 및 GC-MS를 이용하여 배추, 무의 품종별 ; 이들 씨에서 4-8종류의 glucosinolate를 동정하였다. 2. Thymol에 의한 총 glucosinolate의 함량은 배추 및 무에서는 복돌이 배추와 왕관무가 3.21, 2.0$\nu$mole/g으로 가장 많았고 배추씨, 무씨에서는 각각 6.38, 4.07$\nu$mole/g이었다. 3. 배추 및 무의 저장중 총 glucosinolate함량은 저장 기간이 지남에 따라 점차 감소하는 경향이였다. 4. 열처리 시간에 따른 배추와 무의 indoleacetonitrile함량은 열처리 시간이 지남에 따라 증가하며 40분 열처리시 최대치를 나타내었다. 5. indolemethanol이 생성될때 방출되는 배추, 무, 배추씨 및 무씨의 Thiocyanate ion의 함량은 열처리 시간이 지남에 따라 증가하며 40분 열처리시 최대치를 나타내었으며 자가분해에 따른 함량도 분해시간이 경과함에 따라 증가하며 30분경과시는 최대치를 나타내었고 이는 40분 열처리시보다 많은 함량이였다.함량이였다.

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Analysis of Glucosinolates and the Change of Contents during Processing and Storage in Cruciferous Vegetables (십자화과 채소의 가공 및 저장 중 Glucosinolate의 분석과 그 함량변화)

  • Shim, Ki-Hwan;Sung, Nack-Kie;Kang, Kap-Suk;Ahn, Cheol-Woo;Seo, Kwon-Il
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.1
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    • pp.43-48
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    • 1992
  • Glucosinolates, containing indole glucosinates in Chinese cabbage, cabbage, radish and kale were analyzed as 6, 5, 5 and 2 types of glucosinolates, respectively, Four types of glucosinolates from mustard was identified, but indole glucosinolates were not detectable. In Chinese cabbage, the content of glucosinolates showed higher amount in the bottom, stalk and leaf part in the order, Total amounts of glucosinolates, in dry basis, were higher amount in Chinese cabbage, cabbage, radish, kale and mustard in the Order, and also in processing conditions, showed higher amount in fresh, sun dried, oven dried and cooked samples in the order. During the storage in Chinese cabbage and cabbage, total amounts of glucosinolate decreased gradually as time goes by.

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Variation of Glucosinolate Contents among Domestic Broccoli (Brassica oleracea L. var. italica) Accessions (국내 브로콜리(Brassica oleracea L. var. italica) 유전자원 내 Glucosinolate 함량 변이)

  • Lee, Jun Gu;Kwak, Jung-Ho;Um, Yeong Cheol;Lee, Sang Gyu;Jang, Yoon-Ah;Choi, Chang Sun
    • Horticultural Science & Technology
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    • v.30 no.6
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    • pp.743-750
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    • 2012
  • A total of 95 broccoli (Brassica oleracea L. var. italica) accessions were evaluated for the identification of desulfo-glucosinolates and their content variation in the flower head using ultra performance liquid chromatography, to select the potentially functional broccoli breeding lines. The six individual desulfo-glucosinolates, including progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicanapin, and glucobrassicin, were commonly identified, based on the chromatogram peak comparison with those of the nine individual glucosinolate standards. The total glucosinolate contents varied from 4.2 to $29.0{\mu}mol{\cdot}g^{-1}$ DW and the glucoraphanin (1.6 to $13.9{\mu}mol{\cdot}g^{-1}$ DW) was confirmed as a major constituent in the total glucosinolate profile among the six identified individual glucosinolate species, whereas the progoitrin, which was only detected in 13 accessions, showed accession-specific variation and negative correlation with glucoraphanin content. It was also revealed that the four major glucosinolates, such as glucobrassicanapin, glucoraphanin, glucobrassicin, and gluconapin, affected major content variation and showed higher positive inter-correlation. These results might be used for the selection of potential breeding materials as functional broccoli germplasm through the further evaluation on the stability and reproducibility of glucosinolate profile depending on environmental factors or cultural managements using the selected accessions.

Quantitative analysis of glucosinolates and thermal degradation product of indole glucosinolates in radish (무의 glucosinolate와 indole glucosinolate 열분해산물의 함량분석)

  • Shim, Ki-Hwan;Kang, Kap-Suk;Ahn, Cheol-Woo;Seo, Kwon-Il
    • Applied Biological Chemistry
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    • v.36 no.1
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    • pp.23-28
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    • 1993
  • Glucosinolates from radish (Wangkwan, Daejangkun, Taebaek, Daebooryeung and No. 364) and its seed (Wangkwan) were identified, and their degradation product by heat treatment was analyzed. The Wangkwan variety contained much more types of glucosinolates than other radish varieties. Total glucosinolate contents of Wangkwan, Daejangkun, Taebaek, Daebooryeung, No. 354 and Wangkwan seed by GC method were 1.25, 1.10, 0.97, 0.96, 0.90 and 2.14 ${\mu}mole/g$, respectively. The indoleacetonitrile contents after 20 minutes' heat treatment at $100^{\circ}C$ from Wangkwan, Daejangkun, Taebaek, Daebooryeung, No. 364 and Wangkwan seed were 0.28, 0.20, 0.23, 0.21, 0.24 and 0.58 ${\mu}mole/g$, respectively. The heat treatment increased the thiocyanate ion contents in radish and its seed, and the contents were maximum at 30 min.

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Antioxidant and Anti-adipogenic Effects of Kohlrabi and Radish Sprout Extracts (콜라비 새싹 추출물과 무순 추출물의 항산화 및 지방세포 분화 억제 활성)

  • Lee, Young-Jun;Kim, Jae-Hwan;Oh, Ji-Won;Shin, Gi-Hae;Lee, Jong Seok;Cho, Ju-Hyun;Park, Jin-Ju;Lim, Jeong-Ho;Lee, Ok-Hwan
    • Korean Journal of Food Science and Technology
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    • v.46 no.5
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    • pp.531-537
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    • 2014
  • Common cruciferous vegetables, kohlabi (Brassica oleracea) and radish (Raphanus sativus), contain phytochemicals such as glucosinolates and carotenoids. Therefore, this study investigated the antioxidant and anti-adipogenic effects of kohlrabi sprout extract (KSE) and radish sprout extract (RSE). The total carotenoid and glucosinolate contents of KSE and RSE were $39.50{\pm}0.67$ and $76.73{\pm}2.75mg/g$, respectively. The total glucosinolate contents of KSE and RSE were $2.65{\pm}0.02$ and $8.13{\pm}0.54mg/g$, respectively. The in vitro-antioxidative activities of KSE and RSE were significantly increased in a dose-dependent manner. Furthermore, ${\beta}$-carotene and glucosinolate-enriched KSE and RSE significantly inhibited lipid accumulation and reactive oxygen species production during the adipogenesis of 3T3-L1 preadipocytes. These results suggest that glucosinolate-enriched KSE and RSE, especially RSE, can be used in the treatment of obesity and as a natural source of antioxidants.

The Glucosinolate and Sulforaphane Contents of Land Race Radish and Wild Race Radish Extracts and Their Inhibititory Effects on Cancer Cell Lines (재래종 무와 갯무 추출물의 암세포주 증식 저해 활성 및 Glucosinolate와 Sulforaphane의 함량)

  • Choi, Sun-Ju;Choi, A-Reum;Cho, Eun-Hye;Kim, So-Young;Lee, Gun-Soon;Lee, Soo-Seong;Chae, Hee-Jeong
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.4
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    • pp.558-563
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    • 2009
  • The inhibitory effects of land race radish (LRR) and wild race radish (WRR) extracts on cancer cell lines were investigated. A and their glucosinolate and sulforaphane contents were analyzed. The anticancer activitiesy of the LRR and WRR extracts on the breast cancer cell line MCF-7 were determined by a CCK (cell counting kit) assay, in which WWR showed higher inhibition rates than LRR. The sulforaphane content of WRR was higher than that of LRR. In the lung cancer cell line, A-549, WRR showed higher inhibition rates and a higher total glucosinolate content than LRR. The glucosinolate contents of the radishes were analyzed by the Pd-quicktest method, showing that WRR contained more glucosinolate than LRR in both the trunk and root. In conclusion, these results indicate that wild race radish could be used for the quality improvement of radishes.

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Metabolic engineering of aliphatic glucosinolates in Chinese cabbage plants expressing Arabidopsis MAM1, CYP79F1, and CYP83A1

  • Zang, Yun-Xiang;Kim, Jong-Hoon;Park, Young-Doo;Kim, Doo-Hwan;Hong, Seung-Beom
    • BMB Reports
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    • v.41 no.6
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    • pp.472-478
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    • 2008
  • Three Arabidopsis cDNAs, MAM1, CYP79F1, and CYP83A1, required for aliphatic glucosinolate biosynthesis were introduced into Chinese cabbage by Agrobacterium tumefaciens-mediated transformation. The transgenic lines overexpressing MAM1 or CYP83A1 showed wild-type phenotypes. However, all the lines overexpressing CYP79F1 displayed phenotypes different from wild type with respect to the stem thickness as well as leaf width and shape. Glucosinolate contents of the transgenic plants were compared with those of wild type. In the MAM1 line M1-1, accumulation of aliphatic glucosinolates gluconapin and glucobrassicanapin significantly increased. In the CYP83A1 line A1-1, all the aliphatic glucosinolate levels were increased, and the levels of gluconapin and glucobrassicanapin were elevated by 4.5 and 2 fold, respectively. The three CYP79F1 transgenic lines exhibited dissimilar glucosinolate profiles. The F1-1 line accumulated higher levels of gluconapoleiferin, glucobrassicin, and 4-methoxy glucobrassicin. However, F1-2 and F1-3 lines demonstrated a decrease in the levels of gluconapin and glucobrassicanapin and an increased level of 4-hydroxy glucobrassicin.

Sulforaphane is Superior to Glucoraphanin in Modulating Carcinogen-Metabolising Enzymes in Hep G2 Cells

  • Abdull Razis, Ahmad Faizal;Noor, Noramaliza Mohd
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.7
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    • pp.4235-4238
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    • 2013
  • Glucoraphanin is the main glucosinolate found in broccoli and other cruciferous vegetables (Brassicaceae). The objective of the study was to evaluate whether glucoraphanin and its breakdown product sulforaphane, are potent modulators of various phase I and phase II enzymes involved in carcinogen-metabolising enzyme systems in vitro. The glucosinolate glucoraphanin was isolated from cruciferous vegetables and exposed to human hepatoma cell line HepG2 at various concentrations (0-25 ${\mu}M$) for 24 hours. Glucoraphanin at higher concentration (25 ${\mu}M$) decreased dealkylation of methoxyresorufin, a marker for cytochrome P4501 activity; supplementation of the incubation medium with myrosinase (0.018 U), the enzyme that converts glucosinolate to its corresponding isothiocyanate, showed minimal induction in this enzyme activity at concentration 10 ${\mu}M$. Quinone reductase and glutathione S-transferase activities were unaffected by this glucosinolate; however, supplementation of the incubation medium with myrosinase elevated quinone reductase activity. It may be inferred that the breakdown product of glucoraphanin, in this case sulforaphane, is superior than its precursor in modulating carcinogen-metabolising enzyme systems in vitro and this is likely to impact on the chemopreventive activity linked to cruciferous vegetable consumption.

The Change of Thiocyanate (Goitrogen) Amount, Indolylmethyl Glucosinolate Content and Myrosinase Activity in Redish Kimchi during Fermentation (무우김치 숙성 중 thiocyanate(gitrogen)함량, 기질(indolylmethyl glucosinolate) 함량 및 myrosinase 활성도 변화에 관한 연구)

  • Mee Ree Kim;Hei Soo Rhee
    • Korean journal of food and cookery science
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    • v.5 no.1
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    • pp.1-8
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    • 1989
  • The study on the change of general properties of Kagdugi during dfermentation reveals that around the third day of fermentation, optimum for good flavor, the pH decreased to around or below 4 while the acidity increased gradually. The relative amount of thiocyanate in the radish Kimchi homogenate decreased to 73% after 1 day and after 3 days to 32% of control. And the content of indolylmethyl glucosinolate and total myrosinase activity in the tissue of radish Kimchi decreased gradually and on 3rd day to 25% and 4% of control, respectively. On the other hand the concentration of ascorbic acid in the radish Kimchi was found to vary around 1mM. Based on these results, the gradual decline of thiocyanate formation in the radish Kimchi homogenate is concluded to be caused by the gradual decomposition of indolylmethyl glucosinolate and the decline of myrosinase activity, which are directly affected by the change of pH during fermentation.

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