• Title, Summary, Keyword: glutathione S-transferase

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Effect of Garlic on the Hepatic Glutathione S-Transferase and Glutathione Peroxidase Activity in Rat - garlic effect on the glutathione S- transferase and glutathione peroxidase

  • Huh, Keun;Park, Jong-Min;Lee, Sang-Il
    • Archives of Pharmacal Research
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    • v.8 no.4
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    • pp.197-203
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    • 1985
  • It was attempted to observe the effect of garlic on the hepatic glutathione s-transferase and glutathione peroxidase activity in this study. Glutathione s-transferase (EC 2.5.1.18) are thought to play a physiological role in initiating the detoxication of potential alkylating agents, inclnding pharmacologically active compounds. Glutathione peroxidase (EC 1. 11. 1. 9) might play an important role in the protection of cellular structures against oxidative challenge. The activities of glutathione s-transferase and glutathione peroxidase in rat liver were increased by the treatment of garlic juice. Allicin fraction, heat-treated allicin fraction and garlic butanol fraction markedly inhibited glutathione s-transferase activity in vitro, whereas glutathione peroxidase activity was significantly increased in heat-treated allicin fraction and garlic butanol fraction.

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Effect of Garlic (allium sativum) on Glutathione S-Transfer Activity and the Level of Glutathione in the Mouse Liver (마늘이 생쥐간에서 Glutathione S-transferase 활성과 Glutathione 생성에 미치는 영향)

  • Kim, So-Hee;Park, Kun-Young;Suh, Meong-Ja;Chung, Hae-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.3
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    • pp.436-442
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    • 1994
  • The effect of garlic on glutathione S-transferase activity and the level of glutathione in the mouse liver was studied. the intraperitoneal injection of the methanol extract of garlic and ally sulfide which is one of possible active compounds in garlic to ICR mouse before the injection of aflatoxin B1 (AFB1) increased the levels of glutathione and nonprotein-SH in microsomal fraction of the livers. The injection of the chloroform fraction 2 which revealed the highest antimutgenic activity in our previous research in the increase of the activity of glutathione S-transferase and the levels of glutathione and nonprotein -SH. The glutathione itself also had the antimutagenic effect on AFB1 and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA98 and TA100 in vitro.

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Effect of Diallyl Disulfide on the Hepatic Glutathione S-Transferase Activity in Rat : Diallyl Disulfide Effect on the Glutathione S-Transferase

  • Huh, Keun;Lee, Sang-Il;Park, Jong-Min;Kim, Seok-Hwan
    • Archives of Pharmacal Research
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    • v.9 no.4
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    • pp.205-209
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    • 1986
  • Glutathione s-transferase in thought to play a key role in initiating the detoxication of potential alkylating agents, including pharmacologically active compounds. It is widely accepted that garlic contained allin which is converted to allicin by allinase. Allicin is easily degraded to diallyl disulfide and other components. This report attempted to observe the effect of diallyl disulfide on some biological activities. It was observed that the activity of serum transaminase was not changed by the treatment of diallyl disulfide. The liver cytosolic glutathione s-transferase was significantly increased. where as the microsomal glutathione s-transferase was not increased.

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Effect of Sophorae Flos Aqua-acupuncture Solution on the Quinone Reductase and Glutathione S-transferase Activities of Hepa 1c1c7 Cells (괴화약침액이 간세포의 Quinone redutase 와 Glutathione S-transferase 활성에 미치는 영향)

  • Lee Ki-Taek;Lim Jong-Kook
    • Korean Journal of Acupuncture
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    • v.20 no.1
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    • pp.39-43
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    • 2003
  • Sophorae Flos aqua-acupuncture solution(SFAS) was prepared and tested for the induction of quinone reductase and glutathione S-transferase activities and glutathione. SFAS significantly induced QR activity at the concentrations of $0.5{\times},\;1{\times}\;and\;3{\times}$ in cell culture. However, GST activity in murine Hepa 1c1c7 cells was slightly increased with SFAS. SFAS increased GSH levels.

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The Effect of Ginseng on the Hepatic Glutathione S-Transferase Activity (인삼이 간 Glutathione S-Transferase 활성에 미치는 효과)

  • 김낙두;김승희;김신근
    • YAKHAK HOEJI
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    • v.25 no.4
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    • pp.153-160
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    • 1981
  • The investigation aimed to study the effect of ginseng on the hepatic glutathion S-transferase activity. The ginseng methanol extract was administered to rats and mice for 10 days and their hepatic gluthatione S-transferase activities were measured by the method of Habig et al. Glutathione S-transferase activities in the rat treated with 100 and 500mg/kg ginseng methanol extract were increased by 13.4% and 17.10%, respectively and their increases were statistically significant. Similar results were also found in the mouse treated with ginseng 100mg/kg methanol extract. To investigate components of the extract which induce the enzyme, the methanol extract was fractionated into ether and butanol fraction and their effect on the enzyme was compared. Glutathione S-transferase activities in the rat treated with ether fraction were increased by 13.1%, similar to that obtained with ginseng methanol extract, whereas, butanol fraction did not show any increase in the enzyme activities. In the rats treated with maltol, one of the components in ether fraction, 5mg/kg for 10 days, activity of glutathione S-transferase was increased by 7.89%, but its increase was not significantly different from control group. Therefore, it may be concluded that ginseng methanol extract and its ether soluble fraction had effect on the elevation of glutathione S-transferase activities, whereas, butanol fraction of ginseng methanol extract had no effect on the enzyme.

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Catalase, Glutathione S-Transferase and Thioltransferase Respond Differently to Oxidative Stress in Schizosaccharomyces pombe

  • Cho, Young-Wook;Park, Eun-Hee;Lim, Chang-Jin
    • BMB Reports
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    • v.33 no.4
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    • pp.344-348
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    • 2000
  • The logarithmically growing Schizosaccharomyces pombe cells were subjected to high heat ($40^{\circ}C$), hydrogen peroxide, and heavy metals such as mercuric chloride and cadmium chloride. Then, the stress responses of catalase, glutathione S-transferase and thioltransferase were investigated. The high heat and cadmium chloride enhanced the catalase activity. The glutathione S-transferase activity of S. pombe cells was increased after treatments with heavy metals. The thioltransferase activity of S. pombe cells was completely abolished by mercuric chloride. Hydrogen peroxide caused no effect on the activities of glutathione S-transferase and thioltransferase. These results suggest that the response of S. pombe cells against oxidative stress is very complicated.

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Effect of Bromobenzene Pretreatment on the Hepatic Glutathione Content and Glutathione S-transferase Activity in Bromobenzene Treated Rats (흰쥐에 있어서 Bromobenzene전처치가 간조직 중 Glutathione 및 Glutathione S-transferase활성에 미치는 영향)

  • 신중규
    • Journal of Environmental Health Sciences
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    • v.23 no.2
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    • pp.83-88
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    • 1997
  • To evaluate the effect of bromobenzene pretreatment on the bromobenzene metabolism, the animal group was induced the stage of slight liver damage with 7 times bromobenzene injection every two days (400 mg/kg body wt. i.p.). In the present experimental animal model, the single dose of bromobenzene(400 mg/kg body wt. i.p.) was injected to the bromobenzene-pretreated rats and the hepatic aniline hydroxylase(AH) activity, glutathione(GSH) content and glutathione S-transferase (GST) activity were determined at the intervals of 2, 4, 8, 24 hours throughout 24 hr. The activities of hepatic AH and GST were generally higher in bromobenzene-pretreated rats than those in normal group throughout the whole course of experiment. Furthermore, the decreasing rate of hepatic GSH content was also higher in bromobenzene pretreated rats than in normal rats. Moreover, the value of V$_{max}$ in hepatic GST was higher in bromobenzene pretreated rats than that in the normal rats. In conclusion, these results indicate that the pretreatment of bromobenzene may rather enhance the bromobenzene metabolism.

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The Glutathione Peroxidase, Glutathione Reductase and Glutathione-s-Transferase Activity in Liver, Kidney and Testes of Male Rats Intoxicated by Cadmium Chloride and Effect of Leek(Allium Odorum L. ) (카드뮴에 중독된 웅성 흰 쥐의 간, 신장 및 고환의 Glutathione Peroxidase, Glutathione Reduetase, and Glutathione-s-Transferasea의 활성도와 부추의 효과)

  • 안령미
    • Journal of Environmental Health Sciences
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    • v.18 no.1
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    • pp.76-83
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    • 1992
  • Effect of freeze drying leek against cadmium poisoning on glutathione peroxidase, on glutathione reductase and on glutathione-s-transferase in liver, kidney and testes of the male rats during the administered period. In this experiment, male rats of Sprague-Dawley strain were used. The rats which were fed for 15 weeks were divided into 4 groups basal diet 3% leek added diet basal diet and cadmium in water and 3% leek added diet and cadmium in water. Cadmium was administered ad libitum 100ppm CdCl$_{2}$ in distilled water. The followings are the result of this experiment. 1. Leek enhanced the glutathione peroxidase activities which were reduced by cadmium treatment in liver, kidney and testes but not significance. 2. Leek reduced glutathione reductase activities which were incresed by cadmium in liver, kidney and testes. 3. Leek incresed the activities of glutathfone-s-transferase in liver but not in kidney and but not in testes. 4. Leek incresed glutathione concentration which was decresed by cadmium treatment in liver and kidney but not testes. This experiment showed that leek-addition group had protective effect against cadmium poisoning and alleviated GR and glutathione-s-transferase activities in tissues. Leek incresed activities of glutathione peroxidase in liver, kidney and testes but not significance. Therefore, this experiment concluded that leek defensive power against long term cadmium poisoning.

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Induction of NAD(P)H:quinone reductase and glutathione S-transferase by Xanthii Fructus and Prunellae Spica Extracts (창이자 및 꿀풀하고초에 의한 NAD(P)H:quinone reductase와 glutathione S-transferase의 유도)

  • Shon, Yun-Hee;Lee, Ki-Taek;Park, Sin-Hwa;Cho, Kyoung-Hee;Lim, Jong-Kook;Nam, Kyung-Soo
    • Korean Journal of Pharmacognosy
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    • v.32 no.4
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    • pp.269-273
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    • 2001
  • Ethanol extracts from Xanthii Fructus (XFE) and Prunellae Spica (PSE) were investigated for the effects on the induction of cancer chemoprevention-associated enzymes. The following effects were measured: (a) induction of quinone reductase (QR) (b) induction of glutathione S-transferase (GST) (c) reduced glutathione (GSH) level. XFE and PSE were potent inducers of quinone reductase activity in Hepa1c1c7 murine hepatoma cells. Glutathione levels were increased with XFE and PSE. In addition, glutathione S-transferase activity was increased with XFE. However, GST activity was not increased with PSE. These results suggest that XFE and PSE have chemopreventive potentials by inducing quinone reductase and increasing GSH levels.

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Activity of Glutathione S-transferase and Effect of Alcohol Decomposition on the Fruit of Hovenia dulcis Thunb (지구자의 Glutathione S-transferase 활성과 알코올 분해효과)

  • Cha, Bae-Cheon;Lee, Eun-Hee;Lee, Eun;Park, Hang-Ho
    • YAKHAK HOEJI
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    • v.48 no.3
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    • pp.213-217
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    • 2004
  • For the determination of glutathione S-transferase activity, a new method was established by using HPLC system. Moreover, amount of enzyme for a optimum reaction was determined by a comparative study with a variety concentration of enzyme. Using a established method, activity of glutathione S-transferase that is alcohol metabolizing enzyme was investigated on the fruit of Hovenia dulcis Thunb. As the result of experiment, EtOH and $H_2O$ extracts of the fruit of Hovenia dulcis Thunb showed visible a synergistic effect of glutathione S-transferase activity. On a continuous experiment, EtOH and $H_2O$ extracts of the fruit of Hovenia dulcis Thunb showed alcohol decomposition activity on the in vivo test using rat. These results suggest that the fruit of Hovenia dulcis Thunb may be useful in the prevention of hangover.