• Title, Summary, Keyword: granulosa cell tumor

Search Result 22, Processing Time 0.042 seconds

Insights into granulosa cell tumors using spontaneous or genetically engineered mouse models

  • Kim, So-Youn
    • Clinical and Experimental Reproductive Medicine
    • /
    • v.43 no.1
    • /
    • pp.1-8
    • /
    • 2016
  • Granulosa cell tumors (GCTs) are rare sex cord-stromal tumors that have been studied for decades. However, their infrequency has delayed efforts to research their etiology. Recently, mutations in human GCTs have been discovered, which has led to further research aimed at determining the molecular mechanisms underlying the disease. Mouse models have been important tools for studying GCTs, and have provided means to develop and improve diagnostics and therapeutics. Thus far, several genetically modified mouse models, along with one spontaneous mouse model, have been reported. This review summarizes the phenotypes of these mouse models and their applicability in elucidating the mechanisms of granulosa cell tumor development.

A case of canine bilateral ovary granulosa cell tumor and mammary complex carcinoma

  • Chung, Yung-Ho;Hong, Sunhwa;Han, Sang-Jun;Kim, Okjin
    • Korean Journal of Veterinary Service
    • /
    • v.36 no.2
    • /
    • pp.127-132
    • /
    • 2013
  • An 11-year-old poodle bitch was presented for investigation of multicentric mammary masses. Abdominal sonography and radiography demonstrated abnormal enlargement of uterus and ovaries. Blood analysis revealed high progesterone concentration. The ovariohysterectomy and mastectomy were performed. Histopathologically, the mammary masses revealed complex carcinoma-tubulopapillary carcinoma with papillary pattern and tubule pattern. In the uterus, cystic endometrial hyperplasia was observed. Scattered inflammatory cells were observed in the endometrial stroma and mucinous material was protruded from endometrial surface. Also, in the ovaries, bilateral ovary granulosa cell tumor was detected. The bitch made a complete recovery following the ovariohysterectomy and mastectomy. This case was a very rare multiple tumor occurrence with bilateral ovary granulosa cell tumor and mammary complex carcinoma. High progesterone concentration was characterized clinically in the bitch.

Granulosa cell tumor in a sow (돼지의 과립막세포 종양)

  • Kim, Hyeong-Seok;Kang, Sang-Chul;Jung, Ji-YouI;Kim, Hyun-Sup;Kim, Dae-Yong;Kim, Jae-Hoon
    • Korean Journal of Veterinary Research
    • /
    • v.48 no.3
    • /
    • pp.323-326
    • /
    • 2008
  • A two-year-old mixed breed sow was requested to the Veterinary Pathology Laboratory of Cheju National University with a clinical signs of severe abdominal pain and sudden death. Grossly, there was severe hemorrhage in abdominal cavity. Most of internal parenchymas and subcutaneous muscle showed severe pale discoloration. Both ovaries were enlarged with oval to round protruding multilobular masses and dark red in color. And they were firm and contained multiple small cysts in their cut surface. Histopathologically, numerous neoplastic granulosa cells had spherical-to-oval, hyperchromatic nuclei and scant eosinophilic cytoplasms were distributed with follicular pattern in ovarian masses. And the typical Call-Exner bodies, distinctive microcavityies, were observed in the center of small neoplastic follicles. Based on the gross and histopathologic findings, this case was diagnosed as granulosa cell tumor. In our best knowledge, this is believed to be the first report of granulosa cell tumor in a sow in Korea.

Granulosa Cell Tumor of Ovary in a Yorkshire terrier Dog (요크셔테리어에서 발생한 난소의 Granulosa cell tumor)

  • Sung, Ju-Heon;Yoo, Jong-Hyun;Kang, Byeong-Tack;Park, Chul;Jung, Dong-In;Park, Jong-Im;Park, Hee-Myung
    • Journal of Veterinary Clinics
    • /
    • v.24 no.1
    • /
    • pp.38-41
    • /
    • 2007
  • A 12-year-old, intact female Yorkshire terrier was presented with progressive abdominal distention. On radiographic and ultrasonographic evaluation, a large mass was detected in right upper abdomen and it had several discrete anechoic follicles and large cysts. The mass was removed by complete ovariohysterectomy and the resected specimen was histopathologically examined. Based on the gross and histopathologic findings, the mass was definitely diagnosed as malignant granulosa cell tumor (GCT). Until now, the dog has been successfully managed more than a year without complications.

Surgical Treatment for Granulosa Cell Tumor in a Thoroughbred Mare (말에서 Granulosa Cell Tumor에 의한 무발정 치료 예)

  • Seo, Jong-Pil;Son, Won-Gyun;Gang, Su-Jin;Kim, Hyung-Jin;Kim, Dae-Yong;Lee, Choung-Seop;Lee, In-Hyung
    • Journal of Veterinary Clinics
    • /
    • v.26 no.5
    • /
    • pp.486-489
    • /
    • 2009
  • An 8-year-old Thoroughbred mare showing anestrus for longer than 6 months with left ovarian mass was referred to Veterinary Medical Teaching Hospital of Seoul National University. For 6 months, the mare had received 2 times hormonal therapies with $PGF_2{\alpha}$ and progesterone. The mare had not responded to these therapies and not shown estrous behavior. On rectal palpation, the left ovary was enlarged and contralateral ovary was small and inactive. Transrectal ultrasonographic examination of the left ovary revealed a mass composed of multiple, irregularly shaped follicles. Granulosa cell tumor (GCT) was suspected according to her history. Ovariectomy was performed through the left flank laparotomy with a standing position in a simple chute located outside of horse farm. The mass was diagnosed as GCT on histopathology. The mare was recovered without any problem and became pregnant next spring.

Effect of CP-2 Extracted from Coptis and Croton tiglium L. on the Growth and Steroidgenesis of Follicular Granulosa Cells (콥티스속 근경과 탈지된 클로톤 종자의 혼합 추출물(CP-2)이 난포 Granulosa Cell에 미치는 영향)

  • 김종배;김종배;문정조;한영복;김종배
    • Korean Journal of Animal Reproduction
    • /
    • v.17 no.1
    • /
    • pp.13-19
    • /
    • 1993
  • We investigated the effects of CP-2 extracted from the mixture of Copis and Croton tiglium L, which showed very high cytotoxic effect to the various tumor cells, on the growth and steroidenesis of primary and transformed cell lines PA-GS6 and PO-GRS1 by cotransfectionwith SV40 and Ha ras oncogenes. CP-2 inhibited the growth of PA-GS6 and PO-GRS1 in a dose dependent manner when the growth of them was measured by cell number and by protein content, while CP-2 did not affect the growth of primary granulose cells. Productions of progesterone ofprimary and transformed granulosa cells were stimulated by forskolin, but this stimulatory effect was blocked by treatment of CP-2. Clinical application of CP-2 asa new anti-cancer drug and utilization of transformed granulosa cells as a model system for the screening of anti-cancer drug were also discussed.

  • PDF

Parameters for Predicting Granulosa Cell Tumor of the Ovary: A Single Center Retrospective Comparative Study

  • Yesilyurt, Huseyin;Tokmak, Aytekin;Guzel, Ali Irfan;Simsek, Hakki Sencer;Terzioglu, Serdar Gokay;Erkaya, Salim;Gungor, Tayfun
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.15 no.19
    • /
    • pp.8447-8450
    • /
    • 2014
  • Background: To evaluate factors for predicting the granulosa cell tumor of the ovary (GCTO) pre-operatively. Materials and Methods: This retrospective designed study was conducted on 34 women with GCTO as the study group and 76 women with benign ovarian cysts as the control group. Data were recorded from the hospital database and included age, body mass index (BMI), parity, serum estradiol ($E_2$) levels, diameter of the mass, ultrasonographic features, serum CA125 level, risk of malignancy index (RMI), duration of menopause, postoperative histopathology result, and the neutrophil/lymphocyte ratio (NLR). Results: The demographic parameters showed no statistically significant difference between the groups. Preoperative diameter of the mass, CA125, duration of menopause, and neutrophil/lymphocyte ratio were significantly different between the groups. ROC curve analysis demonstrated that diameter of the mass, serum estradiol and Ca125 levels, RMI and NLR may be discriminative factors in predicting GCTO preoperatively. Conclusions: In conclusion, we think that a careful preoperative workshop including diameter of the mass, serum estradiol ($E_2$) and Ca125 levels, RMI and NLR may predict GCTO and may prevent incomplete approaches.

Transcriptome profile of one-month-old lambs' granulosa cells after superstimulation

  • Wu, Yangsheng;Lin, Jiapeng;Li, Xiaolin;Han, Bing;Wang, Liqin;Liu, Mingjun;Huang, Juncheng
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.30 no.1
    • /
    • pp.20-33
    • /
    • 2017
  • Objective: Superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. However, these growing follicles cannot complete maturation and ovulation. Oocyte maturation and competence are acquired during follicular development, in which granulosa cells play an essential role. Methods: In this study, we applied RNA sequencing to analyze and compare gene expression between prepubertal and adult superstimulated follicle granulosa cells in sheep. Results: There were more than 300 genes that significantly differed in expression. Among these differently expressed genes, many extracellular matrix genes (EGF containing Fibulin Like Extracellular Matrix Protein 1, pentraxin 3, adrenomedullin, and osteopontin) were significantly down-regulated in the superstimulated follicles. Ingenuity pathway and gene ontology analyses revealed that processes of axonal guidance, cell proliferation and DNA replication were expressed at higher levels in the prepubertal follicles. Epidermal growth factor, T-Box protein 2 and beta-estradiol upstream regulator were predicted to be active in prepubertal follicles. By comparison, tumor protein P53 and let-7 were most active in adult follicles. Conclusion: These results may contribute to a better understanding of the mechanisms governing the development of granulosa cells in the growing follicle in prepubertal sheep.