• Title, Summary, Keyword: grapefruit seed extract

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Inhibitory Effects of Grapefuit Seed Extract on Growth and Aflatoxin Production of Aspergillus parasiticus (Grapefruit 종자추출물을 이용한 Aspergillus parasiticus의 생육 및 Aflatoxin 생성억제 효과)

  • 조성환;정덕화;서일원;이현숙;황보혜;박우포
    • Journal of Food Hygiene and Safety
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    • v.7 no.1
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    • pp.15-22
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    • 1992
  • This study was conducted to determine the potential of grapefruit seed extract to support Aspergillus parasilicus growth and aflatoxin production. The grapefruit seed extract inhibited the growth and aflatoxin production of the fungi in the level of more than 4,000 ppm and 3,000 ppm in the medium, respectively. Grapefruit seed extract appears to block the conversion of acetate, averufin and versiconal acetate into aflatoxin in vitro experiments. The addition of grapefruit seed extract to the feeding experiment systems did not inhibit the incorporation of 14C-labeled versicolorin A, versicolorin A hemiacetal and sterigmatocystin into aflatoxin. In the electron microscopic examination the biocidal action of grapefruit seed extract was related to the disturbance of cell menbrane funtion, inhibiting cellular respiration.

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Antimicrobial Activity of Grapefruit Seed Extract (자몽 종자 추출물의 항균성)

  • Park, Heon-Kuk;Kim, Sang-Bum
    • The Korean Journal of Food And Nutrition
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    • v.19 no.4
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    • pp.526-531
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    • 2006
  • Minimum inhibition concentration(MIC), growth inhibition activity, and colony forming inhibitory activity of grapefruit seed extract against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Escherichia coli, Salmonella enterifidis and Serratia marcescens were tested. MIC of grapefruit seed extract against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Escherichia coli, Salmonella enteritidis and Serratia marcescens was 12.5, 12.5, 12.5, 50, 50, 100ppm, respectively. Growth inhibition concentration of grapefruit seed extract against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Escherichia coli, Salmonella enteritidis and Serratia marcescens was below 1.0, 6.25, below 1.0, 6.25, 25, 25ppm, respectively. Colony forming inhibitory activity of grapefruit seed extract against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Escherichia coli, Salmonella enteritidis and Serratia marcescens was 93.9, 94.0, 99.9, 4.4, 82.7, 86.4%, respectively. Colony forming inhibitory activities of grapefruit seed extract against Gram positive bacteria were higher than that against Gram negative bacteria.

Antibacterial Effects on Bacillus Stearothermophilus by Adding Natural Grapefruit Seed Extracts in Soymilk (두유에서 자몽씨 추출물의 Bacillus 균에 대한 항균효과)

  • Cho, Kyung Hwan;Park, Soo-Gil
    • Applied Chemistry for Engineering
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    • v.16 no.1
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    • pp.139-143
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    • 2005
  • We evaluated grapefruit seed extract for antibacterial activity at varying time intervals and concentration levels against heat tolerant and spore-forming Bacillus stearothermophilus, mesophilic Bacillus subtilis, and food poisoning Staphylococcus aureus. Grapefruit seed extract showed growth inhibitory activity against B. stearothemophilus and B. subtilis, and S. aureus at the level of 0.01 tp 0.03% in nutrient broth. However, when applied to soymilk in a market, grapefruit seed extract at the level above 0.2% effectively inhibited the growth of B. stearothermophilus, However, it failed to completely sterilize the test organisms. On the other hand, B. subtilis and S. aureus were completely sterilized at the level of 0.2% within 48 hrs and 72 h, respectively. The higher concentration of grapefruit seed extracts showed more effective antibacterial activities against the test organisms, but caused deteriorated organoleptic quality and emulsion stability. We could conclude, by applying grapefruit seed extract (0.015%) with thermal treatment (10 min at $121^{\circ}C$) that the microbial stability of commercial soymilk was enhanced greatly.

Effect of Grapefruit Seed Extract and Ascorbic Acid on the Spoilage Microorganisms and Keeping Quality of Soybean Sprouts (Grapefruit Seed Extract와 Ascorbic Acid의 혼합 처리가 콩나물 변패 미생물과 저장 품질에 미치는 영향)

  • 박우포;조성환;이동선
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.6
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    • pp.1086-1093
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    • 1998
  • The antimicrobial effect of mixed solutions of grapefruit seed extract(250ppm) and ascorbic acid(1%) on the spoilage microorganisms such as Staphylococcus epidermis, Escherichia coli, Pseudomonas syringae, Candida albicans and Corynebacterium xerosis isolated from the spoiled soybean sprouts were investigated. Cell wall and membrane were partially destroyed and the contents of the destroyed cell were exuded after treatment. Packages with 30 m cast polypropylene(CPP), 16 m polyolefin(RD 106) and 10 m high density polyethylene(HDPE) were applied for soybean sprouts dipped in mixed solutions respectively. Oxygen and carbon dioxide concentration inside packages were dependent on the kind of films during storage at 5oC. The antimicrobial activity of mixed solutions was maintained for 5 days at CPP package. Package with HDPE showed a severe browning than the others after 5 days. Ascorbic acid content of mixed solution treatment was higher than that of control for each package.

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Effects of Grapefruit Seed Extract Pretreatment and Packaging Materials on Quality of Dried Persimmons (자몽종자추출물 처리와 포장방법에 따른 반건시 곶감의 품질 변화)

  • Park Hyung-Woo;Cha Hwan-Soo;Kim Sang-Hee;Park Hye-Ran;Lee Seon-Ah;Kim Yoon-Ho
    • Korean Journal of Food Preservation
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    • v.13 no.2
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    • pp.168-173
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    • 2006
  • To investigate the effect of grapefruit seed extract pretreatment and packaging materials on the quality of dried persimmon, dried persimmons were stored for $60{\sim}100$ days at room temperature $(15^{\circ}C)$ after dipping in grapefruit seed extract and then packing with LDPE (low density polyethylene) film and Nylon/LDPE film pouch $(30{\times}30cm^2)$. Weight loss of dried persimmon packaged with Nylon/LDPE film was not exceeded 86% of control. 60% mold occurrence and 50% browness were observed compared to control. There were no significant differences in the firmness, soluble solid content and color between grapefruit seed extract pretreatment and control. Grapefruit seed extracts pretreatment had an effect on the inhibition of color change in Hunters value.

Prevention from Microbial Post-harvest Injury of Fruits and Vegetables by Using Grapefruit Seed Extract, a Natural Antimicrobial Agent -Isolation of antimicrobial substance from grapefruit seed extract- (천연항균제처리에 의한 과채류의 선도유지 및 병해방지에 관한 연구 -Grapefruit 종자추출물로 부터 활성물질의 분리를 중심으로-)

  • Jo, Seong-Hwan;Kim, Gi-Ok;Lee, Geun-Hoe
    • Korean Journal of Food Preservation
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    • v.1 no.1
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    • pp.1-7
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    • 1994
  • To investigate the effect of several bactericides and fungicides against fruit & vegetable decay grapefruit seed extract(GFSE) was tested in a dilution solution. GFSE was shown to be effective against decay and rind breakdown and to extend the shelf-life of fruits and vegetables during storage and tiansport. Antimicrobial efficiency of GFSE on a wide spectra of gram + & - bacteria, moulds and yeasts was demonstrated by the measurement of minimal inhibitory concentrations performed (Bacteria 10-500ppm, Fungi:250-1,000ppm, Yeasts:100-250ppm). GFSE was separated and extracted into water-soluble fraction, water-insoluble and non-dialyzed fraction to isolate the antimicrobial substances. The water-soluble fraction showed the most active antimicrobial effect. The antimicrobial substances were isolated by gas chromatography. As the result of the isolation using GC, Peak-D was found to be the antimicrobial compound in GFSE. The identification of the most antimicrobial substance was carried out by using GC-MS.

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Development and Application of Natural Antimicrobial Agent Isolated from Grapefruit Seed Extract (Grapefruit 종자추출물로부터 광범위 항균제 개발 및 응용에 관한 연구)

  • 조성환;이상열;김재원;고경혁;서일원
    • Journal of Food Hygiene and Safety
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    • v.10 no.1
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    • pp.33-39
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    • 1995
  • The antibacterial and antifungal effect of grapefruit seed extract(GFSE) was investigated for its purpose of application to a diverse spectrum of field as sanitizers, disinfectants and preservatives. GFSE showed coparatively high content of such flavoniods as naringin and hesperidin and ascorbic acid. GFSE containing a low level of organic acids is a heavy viscous and water-soluble liquid. As a result of the antimicrobial test of GFSE, Bacillus subtilis and Aspergillus oryzae did not survive at detectable levels when treated with more than 100 ppm of GFSE. The minimum inhibitory concentrations of GFSE for a wide variety of pathogenic and putrefactive bacteria, fungi and yeasts were 100 ppm and 250 ppm, respectively. In the comparable electron micrograph of microbial cells treated with GFSE or not, we could conclude that GFSE destroy microorganisms by disrupting the functions of the cell wall membrane and microbial spores.

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A Safety Test on Grapefruit Seed Extract (Grapefruit 종자추출물의 안전성 검사)

  • 조성환;고경혁;이근희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.5
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    • pp.690-694
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    • 1995
  • Effects of grapefruit seed extract on the lethality, the primary skin irritation, and the acute eye irritation potentials were evaluated in rats. The acute oral $LD_{50}$ of GFSE for Sprague-Dawley derived rats was 3.75g/kg with 95% confidence limits of 2.55 and 5.52g/kg. Patch application to the intact and abraded skin of rabbits led to mild to moderate erythema and no or mild edema. Application to the eyes of rabbits led to severe corneal opacity, iritis, conjunctival erythema, edema, and discharge.

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Antimicrobial Effect of Grapefruit Seed Extract on Vibrio parahaemolyticus Isolated from the Southern Adjacent Sea of Korea (남해안연안에 분포되어 있는 Vibrio Parahaemolyticus의 성상 및 Grapefruit Seed Extract 처리에 의한 향균효과)

  • 조성환;강동훈;전상수;정덕화
    • Journal of Food Hygiene and Safety
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    • v.9 no.3
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    • pp.141-149
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    • 1994
  • To investigate the distribution of Vibrio parahaemopyticus in the southern 4 coastal areas, Vibrio parahaemolyticus was isolated from seawater, shellfish and sediment from May to October in 1991, and antimicrobial effect of grapefruit seed extract (GFSE) on the growth of isolated strains were examined. In the 120 sample from 4 coastal areas, 16 strains of Vibrio parahaemolyticus were isolated and identified. The distribution serotype of isolated strains was 10 types of monovalent k-antiserum. Especially k-5 and k-28 were highly distribyted with 3 and 4 strains. 31.3% of isolated strains showed positive on Kanagawa phenomenon test. All isolated Vibrio parahaemolyticus were sensitive to chloramphenicol and gentamycin, 5 and 6 strains were resistant to streptomycin and colistin, respectively. Isolated strains were compared with geographical, month and sample. The distribution of 16 isolated Vibrio parahamolyticus was high at Hadong with 50%(8 strains), on July with 43.8%(7 strains) and from seawater with 37.5%(6 strains) respectively. Minimal inhibitory level of GFSE to Vibrio parahaemolyticus was 50 ppm. With 100 ppm treatment of GFSE, the destroy of cell membrane function, outflow of cell ingredients and ghost morphology of cell were investigated.

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The comparative study on Cell Cytotoxicity of H2O2 and Grapefruit Seed extract (콘택트렌즈 보존제 H2O2와 자몽씨 추출물의 세포 독성 비교 연구)

  • Kim, In-Suk;Yoo, Geun-Chang
    • Journal of Korean Ophthalmic Optics Society
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    • v.9 no.1
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    • pp.173-180
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    • 2004
  • This study aims to investigate the effects of hydrogen peroxide and grapefruit seed extract used as a chemical and natural disinfectants on human conjunctival cells in vitro. The main component of grapefruit seed extract is a narigin. It is one of the flavonoid types in citrus fruits and f1avonoids are widely recognized as naturally occurring(삭제) antioxidants. Cytotoxicity was determined by mitochondrial activity(MTT assay) and DNA damage was analyzed by measuring Comet assay. In LDH assay, 5% of grapefruits seed extract has been observed as a material is giving recovery effect of damaged cultured conjuctival cells by hydrogen peroxide. And also, each of concentrations has been treated simultaneously with same amounts and cytotoxicity of hydrogen peroxide and grapefruit seed extract have been estimated by LDH leakage assay after 24 hours. In conclusion, H2O2-induced cytotoxicity, apoptosis were Significantly prevented by grapefruit seed extract. It is a main component of bioflavonoids that we can simply take it as food. The present results suggest that grapefruit seed extract is a useful disinfectanct having antioxidant and antiapoptopic activity as a natural product.

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