• Title, Summary, Keyword: growth enhancer

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Investigation of Germicide and Growth Enhancer Effects on Bean Sprout using NMR-based Metabolomics

  • Yoon, Dahye;Ma, Seohee;Choi, Hyeonsoo;Noh, Hyeonkyung;Ok, Youngjun;Kim, Suhkmann
    • Journal of the Korean Magnetic Resonance Society
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    • v.20 no.4
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    • pp.121-128
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    • 2016
  • Bean sprouts are often cultivated in the circumstances prevailing in the improper using of germicide and growth enhancer. The influence of ingestion those bean sprouts are unknown. The components of the bean sprouts are needed to evaluate for food safety. The extracts of the control, 0.5 g/L germicide, 1 g/L germicide, 12.5 mL/L growth enhancer and 25 mL/L growth enhancer were used to compare the components in the experiment. Nuclear Magnetic Resonance spectroscopy (NMR) was used to analyze the extracts. Statistical analysis of metabolomics showed significant changes between the control and head and the stem of the bean sprouts. Significant changes in metabolites were identified with the bean sprouts cultivated with germicide and growth enhancer by applying qualitative and quantitative analysis. Similar changes in the area of the bean sprouts were observed after treated to germicide and growth enhancer. Although treating germicide and growth enhancer showed no particular harmful metabolites changes to human, it made significant changes in the morphological and the metabolites of the bean sprouts. These changes indicate that the germicide and growth enhancer has substantially potential to influence the growth of the bean sprouts.

Effect of Viral Enhancers on the Tissue-Specific Expression of Bovine Growth Hormone Gene (소성장호르몬 유전자의 조직 특이성 발현에 미치는 바이러스 engancer의 영향)

  • 박계윤;김수미;노정혜
    • Korean Journal of Microbiology
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    • v.27 no.2
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    • pp.85-91
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    • 1989
  • The effect of SV40 and murine cytomegalovirus (MCMV) enhancers on the general and tissue-specific gene expression was investigated. Recombinant plasmids containing these transcriptional engancers downstream of a structural gene for chloramphenicol acetyl transferase (CAT) were constructed. The transient expression of CAT gene from these plasmids was measured in monkey (CV1PD) and HeLa cells. Both SV40 and MCMV engancers activated the expression of CAT gene by more than 20 and 150 folds, respectively, compared with engancerless plasmids. When the SV40 promoter, upstream of CAT gene, was replaced with 2.2 kbp of promoter regulatory region of bovine growth hormone (bGH) gene, there was no expression of CAT even in the presence of enhancers, reflecting the tissue-specific expression of bGH genes. However, when the bGH regulatory region was shortened to 230 bp, the expression level increased dramatically in the presence of SV40 enhancers. In contrast, the expression from the shortened promoter was only marginally activated by the stronger MCMV enhancer.

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Properties of BE0623 to serve as a growth factor of Bifidobacterium

  • Cho, Young Hoon;Sim, Jae Young;Nam, Myoung Soo
    • Korean Journal of Agricultural Science
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    • v.47 no.3
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    • pp.445-457
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    • 2020
  • Prebiotics are defined as "Nondigestible food ingredients that beneficially affect the host by selectively stimulating the growth and activity of bacteria in the intestine" and as defined improve host health. This study was carried out to investigate the effects of bifidobacteria (Bifidobacterium lactis BB12 and Bifidobacterium longum BB536) growth enhancer (BE0623) supplement as a prebiotic. The addition of BE0623, a growth promoting material for bifidobacteria, significantly increased bifidobacteria viable cells counts in fermented milk by about 45 to 75 times compared to the non-added control group. In addition, microscopic observation showed a significant effect on proliferation of bifidobacteria in fermented milk with added BE0623. The viable cell counts in bifidobacteria also increased roughly 102-fold compared to the control group (non-added BE0623) and was higher than that of commercial growth promoters. Each fraction obtained though the purification of BE0623 influenced the increase of bifidobacteria growth. Culturing bifidobacteria with a combination of fractions of BE0623 had a synergistic effect compared to culturing bifidobacteria with each fraction individually. When any of the fractions were not added, the effect of the growth enhancer on bifidobacteria was reduced. These results indicate that all fractions contain substances that promote the growth of bifidobacteria. Therefore, BE0623 is considered to be available as a growth promoting material for bifidobacterium.

Improved Expression of Muscle-derived Growth Hormone Releasing Hormone from ${\alpha}$-Skeletal-muscle Actin Enhancer/Cytomegalovirus Hybrid Enhancer/Promoter

  • Gong, Xia;Meng, Qingyong;Jin, Weiwu;Li, Ning
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.5
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    • pp.784-788
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    • 2007
  • Growth hormone-releasing hormone (GHRH), a hypothalamic neuropeptide can stimulate the growth hormone secretion from the anterior pituitary. In this study, a porcine GHRH expression plasmid pHC-GHRH was used to enhance growth performance through ectopic expressions in muscle tissues of rats. Rats injected with the plasmid of pHC-GHRH and pCMV-GHRH exhibited cumulative weight gains 6.4% and 1% greater than controls. During a 5-day period, significant weight gain differences were observed as follows compared with that of control: during 5-10 days post-injection (DPI) period, the group pHC-GHRH on average 14.5% heavier than controls, $40.73{\pm}0.88$ g vs. $35.57{\pm}1.23$ g (p = 0.0023); during 10-15 DPI period, the group pHC-GHRH on average 13.6% heavier than controls, $37.49{\pm}2.85$ g vs. $33.00{\pm}1.56$ g (p = 0.0146); during 15-20 DPI period, the group pHC-GHRH on average 17.8% heavier than controls, $25.64{\pm}1.39$ g vs. $21.77{\pm}1.27$ g (p<0.05). In addition, plasmids-treated rats maintained higher serum IGF-I than controls. Significant differences of IGF-I were observed on 13 DPI and on 40 DPI in pHC-GHRH group compared with that of controls. This was accomplished through the use of an improved expression cassette that included the cytomegalovirus (CMV) immediate early enhancer/promoter in combination with a 1.5-kilobase portion of porcine ${\alpha}$-skeletal muscle actin promoter.

Isolation of an Algal Growth-enhancer Polysaccharide from the Chlorophyta Monostroma nitidum

  • Cho, Ji-Young;Luyen Hai Quoc;Khan Mohammed N.A.;Shin, Hyun-Woung;Park, Nam-Gyu;Hong, Yong-Ki
    • Fisheries and aquatic sciences
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    • v.9 no.3
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    • pp.115-117
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    • 2006
  • A micro algal growth-enhancing polysaccharide compound was isolated from the green alga Monostroma nitidum using water extraction, molecular fractionation, a DEAE-cellulose column, and fast protein liquid chromatography using a Superose-12 column. The yield of the compound from the seaweed powder was 8.3$\times$l0$^{-3}$%. At 2 mg/mL concentration, the polysaccharide enhanced Tetraselmis suecica cell growth in f/2 medium by approximately 160%.

Iontophoretic Transport of GHRP-6 (성장호르몬방출펩타이드-6 (GHRP-6)의 경피투과)

  • Choi, Bo-Kyung;Oh, Seaung-Youl
    • Journal of Pharmaceutical Investigation
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    • v.31 no.4
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    • pp.273-279
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    • 2001
  • The purpose of this study is to characterize the iontophoretic transport of growth hormone releasing peptides (GHRP-6) through hairless mouse skin from aqueous solution. The effect of various factors, such as pH, poloarity, current profile, current density, current duration, ionic strength, drug concentration, and enhancer application was studied to obtain basic knowledge on the transport. We have also studied the stability of GHRP-6 in solution with/without current. The donor chamber was filled with phosphate buffer solution containing GHRP-6 and the receptor chamber was filled with phosphate buffer solution (pH 7.4). Ag/AgCl electrode was used for their stability and reversibility. At a predetermined time interval, sampling was made and the concentration of drug was analysed using HPLC system. The results showed that, compared to passive flux, the total amount of drug transported increased markedly by the application of anodal current. Cathodal flux was similar to passive flux. Flux increased with the current density, the duration of current application and drug concentration. The effect of enhancers on the flux was studied using hydrophilic (5% N-methyl pyrrolidone) and hydrophobic (5% propylene glycol monolaurate, 5% oleic acid) enhancers. Application of enhancer also increased the flux.

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Effect of Salinity and Micronutrients on the Growth Rate of Bloom from Heterosigma akashiwo (Raphidophyceae) (염분과 미량원소가 Heterosigma akashiwo (Raphidophyceae) 성장률에 미치는 영향)

  • Lee, Juyun;Chang, Man;Han, Myung-Soo
    • Korean Journal of Environmental Biology
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    • v.32 no.2
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    • pp.129-137
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    • 2014
  • In order to understand the Heterosigma akashiwo (Raphidophyceae) bloom, we investigated the cell division pattern of its strains under various physicochemical conditions from three different origin. Among the three tested strains, HYM06HA and NFHTS-AK-1, are belong to South Korea and CCMP452 strain was from USA. Interestingly among three strains, HYM06HA was established from the cyst in Korea. Our study results showed that these three strains were indistinguishable under various salinity and iron (Fe) conditions. All strains were survived at 10 to 40 psu and the growth patterns under different iron (Fe) concentrations were observed to be similar. The growth patterns under different N:P ratios and selenium (Se) concentrations led to classification of strains into Korean and USA strain. Briefly these results indicated that the growth pattern from different strains of H. akashiwo could be dominant using their salinity tolerance. Furthermore nutritional enhancer seemed to be different based on geographical origin.

Toxicological Study on Nonylphenol using the Soil Nematode, Caenorhabditis elegans (토양선충 Caenorhabditis elegans를 이용한 Nonylphenol의 독성 영향 연구)

  • Roh, Ji-Yeon;Choi, Jin-Hee
    • Environmental Analysis Health and Toxicology
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    • v.21 no.4
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    • pp.323-330
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    • 2006
  • The aim of current study was to evaluate the toxicity of nonylphenol(NP) on soil nematode, Caenorhabditi elegans. The stress-related gene expression, growth, reproduction and development have been employed to monitor soil toxicity. The 24-h median effect concentrations $(LC_{50s})$ of NP was $0.15mg/L$. The expressions of vitellogenin-6, vitellogenin-2, cytochrome P450 family protein 35a2 and apoptosis enhancer-1 genes were upregulated in C. elegans by NP exposure. Alterations in growth, reproduction and development were also observed in NP-exposed group and especially hatching failure was observed. The overall results indicate that C. elegans has considerable potential as sensitive markers for NP toxicity monitoring.

Effects of Trampling on Growth and Development in Zoysia japonica (답압이 한국잔디의 생육에 미치는 영향)

  • Seo, Jin Yeol;Chung, Jong Il;Kim, Min Chul;Chung, Jung Sung;Shim, Doo Bo;Song, Seon Hwa;Oh, Ji Hyun;Shim, Sang In
    • Weed & Turfgrass Science
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    • v.4 no.3
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    • pp.256-261
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    • 2015
  • Trampling stress in turfgrass fields caused by traffics often occurs in zoysiagrass (Zoysia japonica) fields including golf course. In order to know the influences of trampling stress on the growth and development of turfgrass, leaf and root growth, chlorophyll fluorescence, chlorophyll content and 2-DE protein analysis were conducted in the turfgrass plants subjected to various levels of trampling stress from 0 to $9,420J\;day^{-1}$ day. Shoot growth was more highly inhibited by trampling stress than root growth. Although root growth was affected by trampling with weak intensity, the highest root length was observed in the turfgrass treated with weak trampling ($1,570J\;day^{-1}$). Chlorophyll fluorescence (Fv $Fm^{-1}$) was drastically lowered by trampling stress with moderate intensity. Leaf number showed similar tendency with leaf greenness. The number was decreased as the trampling intensity was increased. Shoot dry weight was decreased showing a similar tendency with the result of shoot length. The specific protein expressions under weak trampling were related to the functions of stress amelioration. Heat shock 70 kDa protein 10 and oxygen-evolving enhancer protein 1 were the proteins increased positively under trampling stress.