• Title, Summary, Keyword: honeybee

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The Pollination of Honeybee on Peach Blossom Planted in Vinyl House and its Valuation of the Fruits after Harvest

  • Kim, Young-Soo;Cho, Jae-Wook;Lee, Man-Young;Lee, Myeong-Lyeol
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • pp.70-70
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    • 2003
  • The pollination activity of honeybees(Apis melltfera L.) were investigated at the green-house peach blossom of Cheongdo Peach Experiment Station in Korea during the early spring from 2/28/02 - 3/4/02. Furthermore, the differences of ripened fruits, such as bearing ratio, fruit weight, diameter, hardness, sweetness, and acidity were measured to compare the effects with honeybee, artificial, honeybee+artificial, and none pollination. (omitted)

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Dermal and Ocular Irritation Studies of Honeybee (Apis mellifera L.) venom (봉독의 피부자극시험 및 안점막자극시험)

  • Han, Sang-Mi;Lee, Kwang-Gil;Yeo, Joo-Hong
    • Korean Journal of Pharmacognosy
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    • v.42 no.1
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    • pp.76-81
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    • 2011
  • The study of irritation and toxicity of honeybee(Apis mellifera. L) venom collected by a bee venom collector applied topically to the skin and mucous membrane were carried out to prove the safety of honeybee venom in clinical use. Animal for the research was the rabbit and the solution for the test was made from honeybee venom. Six animals were used for the skin test and nine animals were used for the eye mucous membrane test. In results, both tests proved that honeybee venom makes no irritable reaction on skin and eye mucous membrane of rabbit. We consider that this result is helpful for saying about the safety of honeybee venom in clinical use.

Antimicrobial Property of honeybee (Apis mellifera L.) venom against Propionibacterium acnes and Aerobic Skin Flora (국내산 봉독의 여드름 유발균 및 피부 상재균 증식 억제 효과)

  • Han, Sang-Mi;Lee, Kwang-Gil;Yeo, Joo-Hong;Kim, Wan-Tae;Park, Kwan-Kyu
    • Korean Journal of Pharmacognosy
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    • v.40 no.3
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    • pp.173-177
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    • 2009
  • The in vitro antibacterial activities of honeybee(Apis mellifera. L) venom collected by a bee venom collector were investigated against several bacteria including antibiotic-susceptible and resistant Propionibacterium acnes. Honeybee venom was prepared with different concentrations and they showed strong antibacterial activites. Honeybee venom inhibited the growth of the tested antibiotic-resistant P. acnes at the concentration of 1 mg/ml. The inhibitory activities of the honeybee venom showed time-dependent manner. Honeybee venom did not influence the viability of human dermal fibroblast at the high concentration of less than 10 mg/ml. From these results, we expect that honeybee venom has strong antibacterial activities and has advantage for treating cure.

Molecular detection of infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province, Korea (경북 동부지역 꿀벌에서 주요 병원체의 분자생물학적 검출)

  • Ouh, In-Ohk;Do, Jae-Cheul;Seo, Min-Goo;Jeong, Tae-Nam;Cho, Min-Hee;Kwak, Dong-Mi
    • Korean Journal of Veterinary Service
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    • v.36 no.1
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    • pp.37-44
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    • 2013
  • The ecologically and economically important honeybee species are susceptible to infections by various pathogens. This study was investigated to detect infectious pathogens in honeybee colonies reared in eastern Gyeongbuk province by PCR in 2010~2011. A total of 11 infectious pathogens, including 6 viruses, 2 bacteria, 2 fungi, and 1 parasite, were investigated in honeybee colonies suffering from symptoms of sudden collapse, depopulation, or paralysis. The infectious pathogens and infection rates among 24 honeybee colonies detected were as follows: sacbrood virus (66.7%), deformed wing virus (4.2%), black queen cell virus (12.5%), Kashmir bee virus (29.2%), American foulbrood (41.7%), European foulbrood (12.5%), stonebrood (45.8%), chalkbrood (4.2%), and Nosema (33.3%), respectively. Since the coinfection rates of multiple pathogens were detected high in honeybee colonies reared in eastern Gyeongbuk province, large-scale investigation and appropriate control programs need to be established in this region.

Honeybee Toxicity by Residues on Tomato Foliage of Systemic Insecticides Applied to the Soil (침투이행성 농약의 토양처리 후 토마토잎에서의 잔류에 의한 꿀벌 독성)

  • Bae, Chul-Han;Cho, Kyung-Won;Kim, Yeon-Sik;Park, Hyun-Ju;Shin, Kwan-Seop;Park, Yeon-Ki;Lee, Kyu-Seung
    • The Korean Journal of Pesticide Science
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    • v.17 no.3
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    • pp.178-184
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    • 2013
  • Residual toxicity test to honeybee was conducted to evaluate an indirect effects on honeybee after planting hole application of systemic insecticides which were highly toxic to honeybee (Apis mellifera). In this study, It was applied three application rates in the planting hole by three systemic insecticides, dinotefuran GR, imidacloprid GR and clothianidin GR at planting time of tomato. Residual analysis of foliage was carried out after periodic sampling the foliage and investigated the effects of exposed honeybee on the tomato foliage. The honeybee mortality by dinotefuran residues on the foliage was shown almost 100% at 7 days after treatment and decreased 17 days after treatment. The maximum mortality of honeybee by imidacloprid residues on the foliage was 44 ~ 72%. But the effect of pesticide lasted for 18 days and then decreased. The honeybee mortality by clothianidin residues on the foliage was 100% at 7 days after treatment and decreased 14 days after treatment. A tendency of the honeybee mortality and residue in foliage showed a similar character as time goes by. The residues in tomato foliage decreased gradually after 14 days by vigorous growth of tomatoes and the toxic effect of honeybee was significantly decreased after 21 days in actual usage of the treatment.

Bee Venom Therapy of Tail-docked Dog (단미 창상의 생봉독 요법)

  • 최석화;강성수
    • Journal of Veterinary Clinics
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    • v.15 no.2
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    • pp.247-250
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    • 1998
  • This study was designed to examine therapeutic effect of honeybee venom on wound healing in tail-docked dog. Blood samples were taken of jugular vein and hematological values were analyzed in condition of pre,3- and 6-day after canine caudectomy. Apitherapy group was subcutaneously treated with an Italian honeybee(Apis mellifera ligustica). Antibiotic therapeutic group was intramuscularly iaiected with a standard dosage ol'penicluin ,G procaine(20,000 Units/kg of body weight). Changes of leucocyte, erynlrocytel hematocrit, platelet and fibrinogen were not significantly different between bee sting and penicillin injection during wound healing. No changes of wound healing in tine tests, groups were onseren. Whole honeybee venom has been shown to have a antiinflammatory effect in tail-docked dog. It may be concluded that honeybee venom is effective drug being useful far wound healing and disinfection without tissue trauma in dog.

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The Therapeutic Effect of Pigs with Arthritis by Natural Honeybee (Apis mellifera) Venom (생봉독을 이용한 돼지 관절염의 치료효과)

  • 조성구;최석화;최향순;강성수;권영방
    • Journal of Veterinary Clinics
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    • v.16 no.1
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    • pp.145-149
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    • 1999
  • This study was designed to examine therapeutic effect of natural honeybee (Apis mellifera) venom in pigs with arthritis. Pigs were assigned to treated and nontreated control groups. In treated group, 51 pigs were given natural honeybee venom once a day for 5 consecutive days. Acupoints of Sam-li, Jok-su and Pal-ja were stung by the natural honey bees. In control group, 46 pigs were intramuscularly injected with a standard dosage of penicillin G (400,000 IU/kg of body weight) once a day for 5 consecutive days. On day 5 following bee venom therapy, 73.9% of control pigs and 86.2% of pigs in treated group recovered from arthritis. Bee acupuncture therapy did not show any side effects such as allergy, intoxication, hemorrhage, or infection. It might be concluded that honeybee venom therapy was effective in controlling of pig with arthritis.

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The Therapeutic Effect of Natural Honeybee (Apis mellifera) Venom in Adjuvant-induced Arthritic Rat (관절염 유발 랫드에 대한 생봉독의 치료 효과)

  • 강성수;최석화;조성구
    • Journal of Veterinary Clinics
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    • v.16 no.1
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    • pp.155-162
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    • 1999
  • This study was performed to assess that clinco-therapeutic effect of natural Italian honeybee (Apis mellifera) venom in adjuvant-induced arthritic rat. Ninety Sprague- Dawley rats of male were injected with complete Freund's adjuvant (CFA). Adjuvant arthritis was produced by a single subcutaneous injection of 1 mg Mycobacterium butyricum suspended in 0.1 ml paraffin oil into the right hindpaw. Righting reflex was uniformly lost and considered to be the point of arthritis development on day 14 after CFA injection. Experimental groups were divided into three groups. When arthritis was developed in the rat hind-paw, tested groups were administrated with prednisolone (10 mg/kg, p.o) and honeybee venom (one bee, s.c) at an interval of two days. Control group was subcutaneously injected with 0.1 ml of physiological saline solution in the rat at an interval of two days. Clinical findings, hematological values and histopathological findings were observed during or after the drugs administration. In tested groups, the development of inflammatory edema and polyarthritis on day 14 after treatment was suppressed. No significant differences of hindpaw edema volume and lameness score between prednisolone and honeybee venom groups were observed during or after therapeutic drugs treatment. WBC counts of prednisolone and honeybee venom treatment groups as compared with the control group were getting remarkably decreased during or after the therapeutic drugs administration(p<0.01). Erosions of articular cartilage and inflammatory cell infiltrations during or after the therapeutic drugs treatment was effectively suppressed in natural honey venom.

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Origin and effective ingredient standards of honeybee venom as natural antibiotic ingredients (천연항생제로서 봉독의 기원 및 지표성분 설정)

  • Han, SangMi;Kim, JungMin;Han, SangHoon;Yeo, JooHong;Hong, InPyo;Woo, SoonOk;Lee, KwangGill;Kweon, HaeYong
    • Korean Journal of Veterinary Service
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    • v.37 no.2
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    • pp.123-129
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    • 2014
  • This research was performed in order to investigate the origin, standard compound, and structural and physical properties of honeybee venom which used as natural antibiotic ingredients to animal. We compared the nucleotide sequence of mitochondrial cytochrome c oxidase subunit 1 gene (COI) of honeybees were collected from Gangwon, Gyeonggi, Chungnam, Gyeongbuk, Gyeongnam province and Suwon. As major constituent of honeybee venom, melittin was assayed by liquid chromatography. X-ray, differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FT-IR) were utilized to examine the structural and physical properties of honeybee venom. Based on the 627bp sequence of COI, Apis mellifera ligustica was determinated honeybees collected from all six regions. Melittin content varied from 50.7 to 68.6 and averaged 59.8%. According to XRD analysis, honeybee venom showed regular crystal structure peaks at $2{\Theta}=8.5^{\circ}$ and $21.5^{\circ}$. DSC showed that the maximum degration temperature of powder was around $230^{\circ}C$. Through FT-IR analysis, we could identify cross-linking by the presence of peptide peak at 1,500~1,600 $cm^{-1}$. In conclusion, the origin of honeybee venom was Apis mellifera ligustica and effective ingredient standards was melittin content varied from 50.7 to 68.6 as natural antibiotic ingredients.

Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of Korea

  • Park, Yeojin;Noh, Jinhyeong;Seo, Hyun-Ji;Kim, Keun-Ho;Min, Subin;Yoo, Mi-Sun;Yun, Bo-Ram;Kim, Jong-Ho;Choi, Eun-Jin;Cheon, Doo-Sung;Hong, Sung-Jong;Yoon, Soon-Seek;Cho, Yun Sang
    • The Korean Journal of Parasitology
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    • v.58 no.3
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    • pp.257-265
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    • 2020
  • The outbreak of human toxoplasmosis can be attributed to ingestion of food contaminated with Toxoplasma gondii. Toxoplasmosis recently increased in domestic and stray dogs and cats. It prompted studies on the zoonotic infectious diseases transmitted via these animals. Sero- and antigen prevalences of T. gondii in dogs and cats were surveyed using ELISA and PCR, and B1 gene phylogeny was analyzed in this study. Toxoplasmosis antibodies were measured on sera of 403 stray cats, 947 stray dogs, 909 domestic cats, and 2,412 domestic dogs collected at nationwide regions, Korea from 2017 to 2019. In addition, whole blood, feces, and tissue samples were also collected from stray cats (1,392), stray dogs (686), domestic cats (3,040), and domestic dogs (1,974), and T. gondii-specific B1 gene PCR was performed. Antibody prevalence of stray cats, stray dogs, domestic cats, and domestic dogs were 14.1%, 5.6%, 2.3%, and 0.04%, respectively. Antigen prevalence of these animals was 0.5%, 0.2%, 0.1%, and 0.4%, respectively. Stray cats revealed the highest infection rate of toxoplasmosis, followed by stray dogs, domestic cats, and domestic dogs. B1 gene positives were 5 of stray cats, and identified to high/moderate pathogenic Type I/III group. These findings enforce that preventive hygienic measure should be strengthened at One Health level in dogs and cats, domestic and stray, to minimize human toxoplasmosis infections.