• Title, Summary, Keyword: hyaluronic acid(HA)

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The effect of Astragalus membranaceus methanol extract on hyaluronic acid production in HaCaT cells (황기 메탄올 추출물이 HaCaT 세포에서 Hyaluronic acid 생성에 미치는 영향)

  • Lee, Pyeong-Jae;Kim, Hee-Taek;Yoon, Kyung-Sup;Park, Hyun-Chul;Ha, Hun-Yong
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.26 no.1
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    • pp.75-81
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    • 2013
  • Objectives: Hyaluronic acid, high molecular glycosaminoglycan, exists in extracellular matrix of tissue, especially, in skin and has been known to be deeply involved in skin hydration. In this study, we investigated the effect of methanol extract of Hwang-gi, Astragalus membranaceus root, on hyaluronic acid production in human keratinocyte HaCaT cells. Methods: We determined hyaluronic acid synthase 2 gene expression and hyaluronic acid production in HaCaT cells by using RT-PCR and ELISA, respectively. Results: Hwang-gi extract didn't show the toxicity to HaCaT cells within the treated concentration and increased the hyaluronic acid synthase 2 gene expression and hyaluronic acid production. Conclusions: Hyaluronic acid production increased by Hwang-gi could be, partially, contribute to the moisturing effect in skin by it.

An Experimental Study on the Effect of Angelica gigas Ethanol Extract on Hyaluronic Acid Synthesis (Angelica gigas 에탄올 추출물의 Hyaluronic acid 합성 효과에 대한 실험적 연구)

  • Park, Hye-Su;Ha, Hun-Yong;Kim, Hee-Taek
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.31 no.1
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    • pp.32-41
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    • 2018
  • Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and skin aging. The purpose of this study is to examine the effects of Angelica gigas(A. gigas) ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There were no cytotoxicity in $50{\mu}g/ml$ concentration A. gigas extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration A. gigas extract. Hyaluronic acid production was higher than control group in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration A. gigas extract. Conclusions : Hyaluronic acid production was increased by A. gigas extracts. Therefore, We suggest that A. gigas can make a contribution to the moisturizing effect on human skin.

An Experimental Study on the Effect of Angelica acutiloba Ethanol Extract on Hyaluronic Acid Synthesis (일당귀 에탄올 추출물의 Hyaluronic Acid 합성 효과에 대한 실험적 연구)

  • Kang, Min-Seo;Ha, Hun-Yong;Kim, Hee-Taek
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.28 no.1
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    • pp.32-40
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    • 2015
  • Objectives : Hyaluronic acid(HA) is a mucopolysaccharide, occuring naturally in living organisms. It is one of the most hydrophilic molecules, so it has been known as being related to skin hydration and anti-aging. The purpose of this study is to examine the effects of Angelica acutiloba ethanol extract on hyaluronic acid synthesis. Methods : To determine cytotoxicity and hyaluronic acid synthase 2 gene expression, hyaluronic acid production in HaCaT cells, MTT assay and RT-PCR ELISA was used. Results : There was no cytotoxicity in $50{\mu}g/ml$ concentration Angelica acutiloba extract in MTT assay. Hyaluronic acid synthase 2(HAS2) gene expression was increased by all treated concentration Angelica acutiloba extract. Hyaluronic acid production was higher in $50{\mu}g/ml$ & $100{\mu}g/ml$ concentration Angelica acutiloba extract than control group. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin. Conclusions : Hyaluronic acid production was increased by Angelica Acutiloba extracts. Therefore, We suggest that Angelica acutiloba can make a contribution to the moisturing effect on human skin.

Micro-threads of Cross-linked Hyaluronic Acid Hydrogel using a Microfluidic Chip (미세 유체 칩 기반의 히알루론산 미세 실의 제작)

  • Lee, Yun-Kyung;Lee, Kwang-Ho
    • Journal of Biomedical Engineering Research
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    • v.38 no.1
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    • pp.1-8
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    • 2017
  • The successful synthesis of hyaluronic acid micro-threads is very promising approach for the broad application in tissue engineering such as dermal fillers. Because hyaluronic acid has the excellent biocompatibility and ability to maintain the moisture of up to several hundred times its own weight. In order to generate the hyaluronic acid micro-threads in microfluidic system, we employed two-phase flow microfluidic chip to make a rapid synthesis of the hyaluronic acid hydrogel. Hyaluronic acid was mixed with 0.02N NaOH solution and 1, 4-Butanediol diglycidyl ether (BDDE) solution and then injected into core channel. The ethanol was used for the 3-dimensional micro-thread formation in sheath channel. We manipulated the diameter of HA micro-threads using controlling of flow rates in microfluidic chip, and showed the feasibility of immobilization in HA micro-threads with florescent substances. Also, the generated HA micro-threads were evaluated and showed the suitable properties with tensile strength, bending property, and swelling profiles for dermal fillers. As a result, we suggested an innovative method for microfluidic chip-based HA micro-threads which could safely be applied as dermal filler in tissue engineering.

Self-Aggregated Nanoparticles of Lipoic Acid Conjugated Hyaluronic Acid (히알루론산에 결합된 리포산 자기조립체의 제조 및 특성)

  • Hong, In-Rim;Kim, Young-Jin
    • Polymer Korea
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    • v.32 no.6
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    • pp.561-565
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    • 2008
  • Hyaluronic acid (HA) is a natural glycosaminoglycan and is used widely in the pharmaceutical field. Lipoic acid (LA) helps the regeneration of exogenous and endogenous antioxidants such as Vitamin C and Vitamin E as well as glutathione. It also acts as antioxidant indirectly. Hydrophilic HA as a biodegradable and biocompatible polymer was conjugated with hydrophobic LA as an antioxidant to form the graft copolymer. The carboxyl group of HA was modified by adipic acid dihydrazide (ADH). The synthesis of HA-g-LA graft copolymers was characterized by FT-IR, $^1H$-NMR spectroscopy. The conjugates could form the self-assembled nanoparticles in aqueous solution. The particle size and critical aggregation concentration were verified to use the nanoparticle as a carrier fur the hydrophobic material.

Study on the Moisturizing Effects of Puerariae Radix Ethanol Extract (갈근 에탄올 추출물의 피부 보습 효과에 대한 연구)

  • Hong, Chul-Hee;Lee, Sung-Jin
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.30 no.1
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    • pp.106-117
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    • 2017
  • Objectives : 갈근의 피부 보습 효과에 대해 알아보기 위해 본 연구를 진행하였다. Methods : HaCaT 세포주에 갈근 에탄올 추출물을 처리하였고 Retinoic Acid $1{\mu}M$을 양성 대조군으로 사용하였다. MTT assay, RT-PCR, Hyaluronidase enzyme assay, HA-ELISA kit를 통해 Hyaluronic Acid의 합성량, Hyaluronidase 저해율, 농도별 Hyaluronic Acid 생성량을 측정하였다. 더불어 실제로 피부에 도포 시 보습 능력이 있는지 등에 대하여 확인하였다. Results : 갈근 에탄올 추출물은 뚜렷한 세포독성을 나타내지는 않았고, 모든 농도에서 Hyaluronic Acid 합성과 관련된 hyaluronic acid synthase 2 유전자가 발현되었고, 실제로 Hyaluronic Acid가 생성되는 것을 확인하였다. 임상 효능 평가에서는 유의성 있는 결과를 얻을 수는 없었다. Conclusions : 갈근을 한방 외용제로 사용할 때 유의성 있는 효과를 나타낼 수 있을 것으로 기대할 수 있으며, 실험 결과를 토대로 갈근의 최적 사용 농도를 결정하기 위한 추가적 연구와 복합 처방으로 사용했을 경우에 대한 연구가 추가적으로 진행된다면 한방 외용제로서 잠재적인 가치가 있을 것으로 사료된다.

Cellular Interaction of In Situ Chitosan- and Hyaluronic Acid-Based Hydrogel

  • Noh, In-Sup
    • Proceedings of the Polymer Society of Korea Conference
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    • pp.183-183
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    • 2006
  • Hyaluronic acid and chitosan-based poly(ethylene oxide) (HA-PEO and Chitosan-PEO) hydrogels have been employed as unique biomedical polymeric materials with properties such as bioactivity from polysaccharide, biocompatibility of HA and chitosan as well as PEO and control release of bioactive molecules from the hydrogel itself. We here examine in situ hydrogels based on hyaluronic acid and chitosan in terms of their synthesis, mechanical properties, morphologies and in vitro cellular interactions on their surface and inside. In vivo bone regeneration of HA-PEO and Chitosan-PEO hydrogels was compared with in mouse model.

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The Effects of the Fruits of Foeniculum vulgare on Skin Barrier Function and Hyaluronic Acid Production in HaCaT Keratinocytes (HaCaT 세포에서 회향 열매의 피부장벽기능과 hyaluronic acid 생성에 미치는 영향)

  • Yu, Hak Yin;Yang, In Jun;Lincha, V.R;Park, In Sik;Lee, Dong-Ung;Shin, Heung Mook
    • Journal of Life Science
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    • v.25 no.8
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    • pp.880-888
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    • 2015
  • Foeniculum vulgare (FV) has long been used in traditional medicine for the treatment of inflammatory diseases. In addition, it is usually known as an important medicinal and aromatic plant widely used as a carminative, digestive, lactogogue, and diuretic, and for treating respiratory and gastrointestinal disorders. The skin barrier protects against the invasion of pathogens, fends off chemical and physical assaults, and protects against extensive water loss. In this study, the effects of solvent-fractionated FV fruits on strengthening the skin barrier and maintaining moisture, as well as their antifungal activity, were investigated in human keratinocyte (HaCaT) cells. The expression of involucrin, loricrin, filaggrin, hyaluronic acid synthase, human β defensin, and cathelicidin genes and proteins was measured by reverse transcription polymerase chain reaction (RT-PCR) and western blotting. The production of hyaluronic acid was determined by enzyme-linked immunosorbent assay (ELISA). The butanol fraction increased the expression of involucrin and filaggrin. Both the ethyl acetate and the butanol fractions increased hyaluronic acid production by promoting the expression of hyaluronic acid synthase-1. Although the antimicrobial peptides were increased by FV crude extract and its fractions, the samples did not show a significant effect compared to the normal group. These results suggest that the butanol fraction of FV could be very useful in cosmetics for the treatment of dermatological diseases.

Fabrication of hyaluronic acid membrane using electrospinning method and its application as a catalyst support for gold nanoparticles (전기방사를 이용한 히알루론산 멤브레인의 제조 및 금 나노입자 촉매 담체로의 응용)

  • Lee, Namhun;Park, Hyun-Min;Lee, Sang-Myung;Lee, Dae-Won
    • Journal of Industrial Technology
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    • v.38 no.1
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    • pp.15-19
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    • 2018
  • In this study, hyaluronic acid (HA) was electro-spun to fabricate nanofibrous membranes for support materials. Because HA is very hygroscopic, it is difficult to obtain enough viscosity to apply electrospinning method. Therefore, we mixed HA with polyethylene oxide (PEO) to obtain proper viscosity and added adipic acid (ADH) to promote cross-linking of PEO/HA during electrospinning. The morphology of PEO/HA membrane was optimized according to PEO/HA concentration ratio and spinning rate. Finally, we fabricated gold-nanoparticle-embedded PEO/HA membranes and their catalyst activities were evaluated in tetramethylbenzidine (TMB) oxidation.

Anti Photoaging Effects of Hyaluronic Acid following Oral Administration in SKH-1 Hairless Mice (SKH-1 Hairless Mice에서의 히알루론산 경구 투여를 통한 피부 광노화 개선 효과)

  • Lee, Sung-jin;Seo, YoonHee;Yun, Min-kyu;Yu, Heui-jong;Choe, Soo-Young
    • The Korean Journal of Food And Nutrition
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    • v.28 no.6
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    • pp.941-946
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    • 2015
  • Photoaging is the main extrinsic aging factor that is induced due to UVB. Many studies have revealed that application of hyaluronic acid to the skin is effective in healing photoaging. However, the effect of hyaluronic acid through oral administration is unclear. The aim of this research was to investigate the effect on skin photoaging after oral administration of hyaluronic acid. During a ten week study, hyaluronic acid was fed to hairless mice, which were illuminated by UV radiation. After ten weeks, wrinkle indicator and histological changes were determined. Compared with the control group, the sample group had a decrease in wrinkle depth, thickness, and number. Especially, the HA 160 mg/kg group had a similar value of wrinkle depth ($19.44{\pm}0.75{\mu}m$), number ($654.00{\pm}98.34$), and thickness ($1.35{\pm}0.08mm$), when compared with the RA (retinoic acid) group ($19.28{\pm}0.95{\mu}m$, $653.57{\pm}83.54$, and $1.34{\pm}0.07mm$ respectively). Also, the treated group showed improved elastosis and decreased collagen degradation resulting from UV irradiation. Thus, we can conclude that hyaluronic acid has a positive effect in improving skin photoaging induced by UV radiation. Hyaluronic acid therefore has the potential to be an ingredient in skin health function foods.