• Title, Summary, Keyword: hydrogen peroxide

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Effects of Yuldahansotang water extract on Cultured Primary Hippocampal Cell Culture Damaged by Hydrogen Peroxide (열다한소탕(熱多寒少湯) 전탕액(煎湯液)이 Hydrogen Peroxide에 의해 손상(損傷)된 배양(培養) 해마신경세포(海馬神經細胞)에 미치는 영향)

  • Park, Hye-Sun;Kim, Kyung-Yo;Go, Gi-Deok;Kim, Il-Hwan;Lee, Jae-Heung
    • Journal of Sasang Constitutional Medicine
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    • v.14 no.1
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    • pp.79-89
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    • 2002
  • To evaluate the effect of Yuldahansotang(YHT) water extract on cultured hippocampal cell was inhibited by hydrogen peroxide, MTT assay, NR assay, Neurofilament enzymeimmuno assay and DNA synthesis assay were carried out after the cultured hippocampal cells were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of hydrogen peroxide. The results obtained were as follows: 1. Hydrogen Peroxide decreased the survival rate of the cultured hippocampal cells on NR assay and MIT assay. 2. YHT water extract have efficacy of increasing a amount of neurofilament decreased by hydrogen peroxide in cultured hippocampal cells. 3. YHT water extract have efficacy of increasing DNA synthesis decreased by hydrogen peroxide in cultured hippocampal cells. From above the results, It is concluded that YHT has marked efficacy in preventing for the damages by hydrogen peroxide.

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Effects of Red Ginseng-Ejung-tang and White Ginseng-Ejung-tang Water Extract on Hydrogen Peroxide Production in RAW 264.7 Cells (백삼(白蔘)과 홍삼(紅蔘)이 포함된 이중탕(理中湯)의 마우스 대식세포 내 hydrogen peroxide 생성에 미치는 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.1
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    • pp.78-83
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    • 2011
  • The purpose of this study is to investigate whether the intracellular hydrogen peroxide productions of mouse macrophage RAW 264.7 are modulated by Red Ginseng-Ejung-tang water extract (ER) and White Ginseng-Ejung-tang water extract (EG). Red Ginseng-Ejung-tang were composed of Red Ginseng, Atractylodes rhizome white, Zingiberis Rhizoma Siccus, and Glycyrrhizae Radix. White Ginseng-Ejung-tang were composed of White Ginseng, Atractylodes rhizome white, Zingiberis Rhizoma Siccus, and Glycyrrhizae Radix. The intracellular hydrogen peroxide productions were measured by dihydrorhodamine 123 assay with spectrofluorometer (excitation 485 nm; emission 535 nm). For 4, 20, 24, 44, 48, 68, and 72 h incubation, ER significantly increased hydrogen peroxide productions of RAW 264.7 at the concentration of 25, 50, 100, and $200{\mu}g/mL$ (P <0.05). EG for 4, 20, 24, 44, and 48 h incubation significantly increased hydrogen peroxide productions of RAW 264.7 at the concentration of 25, 50, 100, and $200{\mu}g/mL$ (P <0.05). For 68 and 72 h incubation, EG at the concentration of 50, 100, and $200{\mu}g/mL$ significantly increased hydrogen peroxide productions in RAW 264.7 (P <0.05). These results suggest that ER and EG have the immune-enhancing properties related with their increasing effects on the intracellular hydrogen peroxide production of macrophage.

Inhibitory Effect of Staphylococcus aureus and Bacillus cereus by Lactic Acid and Hydrogen Peroxide (Staphylococcus aureus와 Bacillus cereus에 대한 유산과 과산화수소의 증식억제 효과)

  • Jang Jae Seon;Go Jong Myeong;Kim Yong Hee
    • Journal of Environmental Health Sciences
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    • v.31 no.2
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    • pp.115-119
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    • 2005
  • This study was performed to investigate inhibitory effect on growth of Staphylococcus aureus and Bacillus cereus in lactic acid, hydrogen peroxide and combination of lactic acid and hydrogen peroxide. The minimun inhibitory concentration (MIC) of lactic acid in Staphylococcus aureus were 2500 ppm at pH 7.0, 1250 ppm at pH 5.5, 6.0 and 6.5, while in Bacillus cereus 625 ppm at pH 5.5 and 6.0, 1250 ppm at pH 6.5 and 7.0, respectively. MICs of hydrogen peroxide in Staphylococcus aureus were 50 ppm at pH 6.0, 75 ppm at pH 6.5 and 7.0, while in Bacillus cereus was 75 ppm at pH 5.0, 5.5 and 6.0, respectively. MICs of combined treatment of lactic acid and hydrogen peroxide in Staphylococcus aureus were 1250 ppm of lactic acid with 25 ppm of hydrogen peroxide and 625 ppm of lactic acid with 50 ppm of hydrogen peroxide. When Bacillus cereus were with 1250 ppm of lactic acid with 50 ppm of hydrogen per-oxide and 625 ppm of lactic acid with 75 ppm of hydrogen peroxide at 6.5. The correlations between MICs of lactic acid and hydrogen peroxide in S. aureus and B. cereus obtained through the coefficient of determination ($R^2$). $R^2$ value were 0.9934 and 0.9986, respectively. The inhibitory effect of lactic acid and hydrogen peroxide in S. aureus and B. cereus could be confirmed from the result of this experiment.

Protective Effects of Cornu Saigae Tataricae Extracts on Cultured Spinal Motor Neurons Damaged by Oxygen Free Radical (산소자유기에 의한 척수운동세포 독성에 대한 영양각 추출물의 방어효과)

  • Kang Gil Seong;Kwon Kang Beom;Ryu Do Gon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.5
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    • pp.1202-1207
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    • 2003
  • In order to clarify the neuroprotective effect of Cornu Saigae Tataricae(CST) water extract on cultured mouse spinal motor neuron damaged by hydrogen peroxide (H₂O₂), MTT [3-(4,5-dimethylthiazole-2-yl)- 2,5-diphenyltetrazolium bromide] assay, LDH (Lactate Dehydrogenase) activity assay and SRB (Sulforhodamine B) assay were carried out after the cultured mouse spinal motor neuron were preincubated with various concentrations of CST water extract for 3 hours prior to exposure of hydrogen peroxide Cell viability of cultured mouse spinal motor neurons exposed to various concentrations of hydrogen peroxide for 6 hours was decreased in a dose-dependent manner. MTT50 values were 40 uM hydrogen peroxide. Cultured mouse spinal motor neurons in the medium containing various concentration of hydrogen peroxide for 6 hours showed increasing of LDH activity and decreasing of total protein synthesis. We know that hydrogen peroxide was toxic on cultured spinal motor neurons. Pretreatment of CST water extract for 3 hours following hydrogen peroxide prevented the hydrogen peroxide-induced neurotoxicity such as increasing of LDH activity and decreasing of total protein synthesis. These results suggest that hydrogen peroxide shows toxic effect on cultured spinal motor neurons and CST water extract is highly effective in protecting the neurotoxicity induced by hydrogen peroxide.

Resistant Activity to Hydrogen Peroxide of Lactobacillus spp., Bifidobacterium spp., Bacillus coagulans and Hydrogen Peroxide Generation Capability of Lactobacillus spp. (Lactobacillus spp., Bifidobacterium spp. 및 Bacillus coagulans의 과산화수소 저항성과 과산화수소 생성 능력)

  • Lee, Jong-Hyeok;Yoon, Yeong-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.22 no.2
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    • pp.107-112
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    • 2004
  • Studies on the resistance of Lactobacillus ssp., Bifidobacterium spp. and Bacillus coagulans to hydrogen peroxide were conducted by determination of the viable cells after the test cells in 2mM hydrogen peroxide solution for a predetermined time; L. acidophilus CU4111 and L. casei CU4114 were most resistant to the hydrogen peroxide among the fifteen test lactobacilli strains, whereas L. brevis Cu4206 was the strain which was the most susceptible to hydrogen peroxide. Bifidobacterium longum Cu4131 was one of the resistant strains. A prominant tendency found out that Bacillus coagulans possessed a strong resistance to hydrogen peroxide. The results of level of hydrogen peroxide determination in the cell extracts showed all the test strains contained hydrogen peroxide in the cytoplasm, the amount varied depending on the strain and species of lactic acid bacteria. Bifidobacterium bifidum CU 4134 and L. casei CU 4114 were potent hydrogen peroxide producer strain.

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Effect of Emodin on Hydrogen Peroxide Production in Polyinosinic-Polycytidylic acid-induced Human Neuroblastoma SH-SY5Y Cells (Emodin이 polyinosinic-polycytidylic acid로 유발된 인간 신경모세포종 SH-SY5Y의 hydrogen peroxide 생성증가에 미치는 영향)

  • Lee, Ji-Young;Kim, Young-Jin;Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.6
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    • pp.1039-1043
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    • 2011
  • The purpose of this study is to investigate the modulatory effect of emodin on hydrogen peroxide production in human blastoma SH-SY5Y cells induced by the synthetic analog of double-stranded RNA [polyinosinic-polycytidylic acid]. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. Emodin significantly inhibited the polyinosinic-polycytidylic acid (PIC)-induced production of hydrogen peroxide for 0.5, 2, 12, 18, and 24 hr incubation at the concentrations of 5, 10, 25, and 50 uM in SH-SY5Y (P < 0.05) in dose dependent manner. These results suggest that emodin has neuroprotective property related with its inhibition of hydrogen peroxide production in PIC-induced neuronal cells.

Development of a Hydrogen Peroxide Rocket Engine Facility

  • Ahn, Sang-Hee;S. Krishnan;Lee, Choong-Won
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • pp.131-136
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    • 2004
  • The ongoing developmental studies on the application of hydrogen peroxide for propulsion are briefly reviewed. A detailed design-study of a laboratory scale facility of a hydrogen peroxide mono-propellant engine of 100-N thrust is presented. For the preparation of concentrated hydrogen peroxide, a distillation facility has been realized. Results of water analogy tests are presented. Initial firings using the concentrated hydrogen peroxide were not successful. Low environmental temperature, low contact area of the catalyst pack, and contamination in the hydrogen peroxide were considered to be the reasons. Addressing the first two points resulted in successful firing of the rocket engine.

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동전기-패턴 공정에서 토양내 과산화수소의 거동 이해

  • 박지연;김상준;이유진;양지원
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • pp.191-194
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    • 2004
  • The concentration of hydrogen peroxide in soil and effluent was measured to understand the relationship between the profile of hydrogen peroxide and the removal efficiency of phenanthrene in an Electrokinetic-Fenton process. Electrokinetic phenomena were observed in two different conditions for 1, 2, 4, and 7 days; 3.5% and 10% hydrogen peroxide. The concentration of hydrogen peroxide in soil was high near the anode and decreased towards the cathode due to the direction of electroosmosis. The hydrogen peroxide concentration in effluent increased with time, but the value was lower than the initial. The removal efficiency of phenanthrene at 10% hydrogen peroxide was higher than the case at 3.5%. The removal efficiency after 7 days was high(> 97%) in both cases.

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Removal of Toxic Organic Compound using Electro-Fenton Reaction (Electro-Fenton 반응을 이용한 유독성 유기화합물 처리)

  • Park, Sang Won
    • Journal of Environmental Science International
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    • v.13 no.6
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    • pp.551-560
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    • 2004
  • The feasibility and efficiency of the hydrogen peroxide produced by an electrolysis cell reactor was investigated, From regulating voltages for the given reaction time, the concentration of the hydrogen peroxide was gradually increased with increasing voltages. Optimal voltage range was found to be 10~15 V. The concentration of hydrogen peroxide was much higher with oxygen gas than without oxygen gas in the cathodic chamber. But there was a little difference in the generating rate of hydrogen peroxide regardless of the presence of nitrogen gas. Under given conditions, the maximum value of ICE(Instantaneous Current Efficiency) was about 38%, and then current density was 74 $mA/\textrm{cm}^2.$ The specific energy consumption was $0.694[kWh/kg-H_2O_2].$ Since Esp (Specific Energy Consumption)was very little value, It did not demand high energy in this system. Using the hydrogen peroxide gained in the experiment, Fenton's reaction was conducted and the removal of nitrobenzene, 3-chlorophenol and dye wastewater was studied. This results were very similar to the Fenton's reaction by using commercial hydrogen peroxide.

Effect of Sacchromyces cerevisiae-Fermented Sophorae Radix on Production of Hydrogen Peroxide and Nitric Oxide from Macrophage Treated with Nictoine (Nicotine으로 유발된 대식세포의 hydrogen peroxide와 Nitric Oxide 생성억제에 대한 효모균발효고삼 추출물의 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.5
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    • pp.1049-1054
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    • 2009
  • The effect of Sacchromyces cerevisiae-Fermented Sophorae Radix water extract (SFS) on production of hydrogen peroxide and nitric oxide (NO) from mouse macrophage Raw 264.7 Cells treated with nicotine (1 mM) was investigated through this study. SFS (0, 25, 50, 100, 200, 400 ug/mL) was simultaneously treated with nicotine (1 mM) during culture of 4, 20, 24, 44, 48, 68, and 72 hr. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. NO production after 24 hr treatement was measured with Griess reagent assay. SFS restored the production of hydrogen peroxide and NO reduced by nicotine (1 mM) in Raw 264.7 Cells. These results suggests that SFS could be supposed to have the immunological activity concerned with macrophage's oxidative burst including hydrogen peroxide and NO.