• Title, Summary, Keyword: hydrogen peroxide

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Effect of Oroxylin A on Hydrogen Peroxide Production in Polyinosinic-Polycytidylic acid-induced TM4 Mouse Testis Sertoli Cells (Oroxylin A가 polyinosinic-polycytidylic acid로 유발된 생쥐 서톨리세포 TM4의 hydrogen peroxide 생성증가에 미치는 영향)

  • Park, Wan Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.28 no.4
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    • pp.384-389
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    • 2014
  • The purpose of this study is to investigate the modulatory effect of oroxylin A on hydrogen peroxide production in TM4 mouse testis sertoli cells induced by the synthetic analog of double-stranded RNA [polyinosinic-polycytidylic acid]. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. Oroxylin A significantly inhibited the polyinosinic-polycytidylic acid (PIC)-induced production of hydrogen peroxide for 0.5, 2, 12, 18, and 24 hr incubation at the concentrations of 5, 10, 25, and $50{\mu}M$ in TM4 (P < 0.05) in dose dependent manner. These results suggest that oroxylin A has a protective effect against PIC-induced cellular toxicity with its inhibition of hydrogen peroxide production in PIC-induced sertoli cells.

Effect of Wogonin on Hydrogen Peroxide Production in Polyinosinic-Polycytidylic acid-induced Human Neuroblastoma SH-SY5Y Cells (Wogonin이 polyinosinic-polycytidylic acid로 유발된 인간 신경모세포종 SH-SY5Y의 hydrogen peroxide 생성증가에 미치는 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.26 no.4
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    • pp.491-496
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    • 2012
  • The purpose of this study is to investigate the modulatory effect of wogonin on hydrogen peroxide production in human blastoma SH-SY5Y cells induced by the synthetic analog of double-stranded RNA [polyinosinic-polycytidylic acid]. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. Wogonin significantly inhibited the polyinosinic-polycytidylic acid (PIC)-induced production of hydrogen peroxide for 0.5, 2, 12, 18, and 24 hr incubation at the concentrations of 10, 25, and 50 ${\mu}M$ in SH-SY5Y (P < 0.05) in dose dependent manner. These results suggest that wogonin has neuroprotective property related with its inhibition of hydrogen peroxide production in PIC-induced neuronal cells.

Effect of Scutellariae Radix Water Extract on Hydrogen Peroxide Production in LPS-induced RAW 264.7 Mouse Macrophages (황금(黃芩) 물추출물이 LPS로 유발된 마우스 대식세포의 hydrogen peroxide 생성에 미치는 영향)

  • Park, Wan-Su
    • Korean Journal of Acupuncture
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    • v.28 no.3
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    • pp.33-41
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    • 2011
  • Objectives : The purpose of this study is to investigate effects of Scutellariae Radix water extract on hydrogen peroxide production in LPS-induced RAW 264.7 mouse macrophages. Methods : Scutellariae Radix produced from South Korea (SK) and Scutellariae Radix produced from China (SC) were extracted by hot water. Effects of SK and SC on hydrogen peroxide production in LPS-induced RAW 264.7 were measured by dihydrorhodamine 123 assay after 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL. Results : SK significantly increased hydrogen peroxide production in LPS-induced RAW 264.7 cells for 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). But SC did not represent any significant effect on hydrogen peroxide production in LPS-induced RAW 264.7 cells. Conclusions : These results suggest that Scutellariae Radix, especially produced from South Korea, has the immune-enhancing property related with its increasement of bacteriocidal hydrogen peroxide production in LPS-induced macrophages.

Effects of bleaching time and hydrogen peroxide concentration on hair damage (탈색시간과 과산화수소 농도에 의한 모발의 손상)

  • Kim, Chung-Wun;Chun, Hong-Sung
    • Korean Journal of Human Ecology
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    • v.14 no.3
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    • pp.433-439
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    • 2005
  • In this study, we investigated the morphological and physical changes of hair after bleaching treatments with different concentration of hydrogen peroxide in bleaching agent (3, 6, 9, or 12%) and various treatment time (10, 30, or 60 minutes). Tresses of virgin black hair were bleached using a commercial ammonium persulfate-hydrogen peroxide solution. The tensile strength of virgin hair treated with a bleaching agent depended on the concentration of hydrogen peroxide and the treatment time. As the concentration of hydrogen peroxide in bleaching agent went high and the treatment time increased, the tensile strength of the hair increased but the extension degree decreased. The virgin hairs which were not treated by bleaching agent showed morphologically healthy cuticle, whereas the bleached hairs showed swelled and damaged cuticle patterns. The swelling of the hair and the breaks of the cuticles increased positively by the concentration of hydrogen peroxide and the treatment time. These results suggest that exposure to more concentrated hydrogen peroxide with other bleaching components may lead to more severe hair damage.

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Ultraviolet Microscopic Study on Lignin Distribution in the Fiber Cell Wall of BCTMP

  • Yoon, Seung-Lak;Yasuo Kojina
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.36 no.1
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    • pp.61-66
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    • 2004
  • Bleached chemithermomechanical pulp (BCTMP) was produced from CTMP of Betula maximowicziana Regel by two staged ozone-hydrogen peroxide bleaching in order to improve the optical properties of high yield pulp. This pulp was used for the evaluation of optical properties improvement, chemical characteristics of lignin in fiber and the relationship between lignin and optical properties in fiber cell wall. Hydrogen peroxide treatment improved the brightness, but the post color number (PC No.). There was little improvement on optical properties by ozone treatment, but this could be improved more by using two staged ozone-hydrogen peroxide bleaching. The hydrogen peroxide treatment did not make any change on chemical characteristics of lignin in cell wall, but by ozone treatment, it was found that the non-aromatic conjugated structure was existed in the surface of cell wall, but this could be removed by hydrogen peroxide treatment in two staged ozone-hydrogen peroxide treatment. Therefore, the optical properties was significantly improved, due to the removal of non-aromatic conjugated structure.

Effects of Sintongchukeo-tang on the Cultured Spinal Sensory Neurons Injured by Hydrogen Peroxide (신통축어탕(身痛逐瘀湯)이 Hydrogen Peroxide에 의해 손상(損傷)된 배양(培養) 척수감각신경세포(脊髓感覺神經細胞)에 미치는 영향(影響))

  • Lee, Kye-Seung;Na, Young-Hoon;Cha, Yong-Suk;Heo, Yun;Kim, Do-Hwan;Han, Sang-Hyok;Park, Byong-Min;Lee, In;Moon, Byung-Soon
    • The Journal of Internal Korean Medicine
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    • v.22 no.4
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    • pp.557-565
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    • 2001
  • Objectives : This study was carried out to examine toxic effect of Sintongchukeo-tang on cultured mouse spinal sensory neurons inhibited by neurotoxicity induced by hydrogen peroxide. Methods : MTT assay, NR assay, LDH and neurofilament assay were performed after spinal sensory neurons were preincubater with various concentrations of Sintongchukeo-tang water extract before treatment of cells with hydrogen peroxide. Results : Hydrogen peroxide induced ceil degeneration such as the decrease of cell viability was measured by MTT and NR assay in the cultured mouse spinal sensory neurons. Sintongchukeo-tang water extract was effective in the decrease of LDH activities of neurons produced by hydrogen peroxide. Sintongchukeo-tang water extract was effective in the increase of amount of neurofilaments damaged by hydrogen peroxide. Conclusions : From the above results, it is suggested that hydrogen peroxide induces the inhibition of cell viability in cultured mouse spinal sensory neurons and Sintongchukeo-tang water extract was effective in cultured neurons damaged by hydrogen peroxide.

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The Influence of Hydrogen Peroxide Treatment on Water Stress, Photosynthesis and Thermotolerance of Cucumber(Cucumis sativus) in Greenhouse Cultivation during Summer (Hydrogen Peroxide 처리가 여름철 시설오이의 수분 스트레스, 광합성, 내서성에 미치는 영향)

  • Woo Young-Hoe;Kim Hyung-Jun;Kim Tae-Young;Kim Ki-Deog;Huh Yun-Chan;Chun Hee;Cho Ill-Hwan;Nam Yooun-Il;Ko Kwan-Dal;Lee Kwan-Ho;Hong Kue-Hyon
    • Protected Horticulture and Plant Factory
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    • v.15 no.1
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    • pp.47-52
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    • 2006
  • This studies were carried out in summer season to increase high temperature tolerance using hydrogen peroxide treatments on cucumber in greenhouse. The water stress of cucumber in greenhouse by the hydrogen peroxide treatments showed as control>250 mM>500 mM treatments in order. The photosynthesis rate of cucumber at $30^{\circ}C$ did not show difference with each hydrogen peroxide treatment in temperature controlled greenhouse. However, the photosynthesis rate of cucumber in the control and hydrogen peroxide treatments at $40^{\circ}C$ was significantly different. The photosynthesis rate of cucumber in combined treatment with 1,000 $mg{\cdot}L^{-1}\;CO_2$ supply and hydrogen peroxide was also higher than control, however, there was no different of photosynthesis in 250 mM and 500 mM treatment. The value of $F_v/F_m$ and $F_m/F_o$ of chlorophyll fluorescent in 500 mM hydrogen peroxide treatment at $40^{\circ}C$ was highest. Also the activity of POD, the antioxidant enzyme, was higher with high hydrogen peroxide concentration than the other treatments. The high temperature limits for growth were $43^{\circ}C$ in the control, $44^{\circ}C$ in the 250 mM and $46^{\circ}C$ in the 500 mM according to analyze chlorophyll fluorescent $F_o$. The high temperature tolerance in cucumber increased approximately $3^{\circ}C$ by the hydrogen peroxide treatments under this experiment conditions.

The Effect of Hydrogen Peroxide-Treated Metallothionein on the Hepatic Xanthine Oxidase Activity

  • Huh, Keun;Shin, Uk-Seob;Lee, Sang-Il
    • BMB Reports
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    • v.28 no.6
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    • pp.490-493
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    • 1995
  • We investigated the effect of hydrogen peroxide-treated metallothionein on the hepatic xanthine oxidase activity in vitro. When the metallothionein was preincubated with 1 mM of hydrogen peroxide, the activity of xanthine oxidase and type conversion were elevated dose-dependently by the addition of metallothionein into the reaction mixture. While increasing the treatment of hydrogen peroxide to the $50{\mu}g$of metallothionein, the xanthine oxidase activity and type conversion ratio were remarkably elevated dose dependently compared to the control. When cadmium ion was added to the reaction mixture, the increasing pattern of the enzyme activity was similar to the effect of hydrogen peroxide-treated metallothionein. DTT or penicillamine restored the increasing activity and type conversion of xanthine oxidase by the cadmium ion to the control level.

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Effect of Artemisiae Argi Folium Fermented with Lactobacillus Pentosus on Hydrogen Peroxide Production of Human Hepatocyte Treated with Toxicants (Gallic acid 등으로 유발된 인간 간 조직세포 내 hydrogen peroxide 생성억제에 대한 유산균발효애엽 추출물의 영향)

  • Park, Wan-Su;Kim, Do-Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.6
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    • pp.1379-1384
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    • 2009
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Lactobacillus pentosus (AFL) on hydrogen peroxide production within human hepatocyte HepG2 cells treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. AFL (0~400 ug/mL) was treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFL showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde in HepG2 Cells. AFL could be supposed to have the hepatoprotective effect related with hepatocytologic signaling activity against gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.

Effect of Artemisiae Argi Folium Fermented with Sacchromyces Cerevisiae on Hydrogen Peroxide Production of Human Hepatocyte Treated with Toxicants (Nicotine 등으로 유발된 인간 간조직세포 내 hydrogen peroxide 생성억제에 대한 효모균발효애엽 추출물의 영향)

  • Park, Wan-Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.1
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    • pp.96-101
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    • 2010
  • The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Sacchromyces cerevisiae (AFS) on hydrogen peroxide production within human hepatocyte HepG2 cells treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. AFS (0~400 ug/mL) was treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFS showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde in HepG2 Cells. AFS could be supposed to have the hepatoprotective effect related with hepatocytologic signaling activity against gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.