• Title, Summary, Keyword: immuno-activity

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Anti-tumor and immuno-stimulating activity of fungal polysaccharides

  • Lee, Jae-Hoon
    • The Microorganisms and Industry
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    • v.20 no.3
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    • pp.14-21
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    • 1994
  • Low molecular weight molecules and high molecular weight substances were found to have anti-tumor and immuno-modulating activity. Previously polysaccharides have been received much attention because of adhesives, food additives or animal foods (Whistler et al., 1976). In effort of developing new anti-tumor substances with low toxicity, numerous polysaccharides from yeast, algae, bacteria, higher plants and especially fungi have been investigated for anti-tumor and immuno-modulating activities. Thus the high molecular weight molecule was reported to have anti-tumor activity through host mediated immunity. In this brief article, attention will be paid to polysaccharides which is especially fungal origin.

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Immuno-stimulating, Anti-stress and Anti-thrombotic Effects of Unossified Velvet Antlers

  • Shin, Kuk-Hyun;YunChoi, Hye-Sook;Lim, Soon-Sung;Won, Do-Hee;Kim, Jae-Kun
    • Natural Product Sciences
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    • v.5 no.1
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    • pp.54-59
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    • 1999
  • Both ethanol and water extracts of unossified velvet antler were found to exhibit a significant immuno-stimulating activity as measured by carbon clearance test in mice, a remarkable anti-fatigue effect in weight-loaded forced swimming performance in mice, a significant anti-stress effect on immobilization in rats. The antler extracts also showed a weak but significant anti-thrombotic activity. These findings are indicative of adaptogenic properties of antlers and their normalizing effects during stressful condition.

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Antifungal Activity of Glycycoumarin to Candida albicans (Glycycoumarin 감초성분의 항진균효과)

  • Lee, Jue-Hee;Lee, Young-Mi;Han, Yong-Moon
    • YAKHAK HOEJI
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    • v.55 no.3
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    • pp.234-239
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    • 2011
  • Glycycoumarin, a 3-arylcoumarine isolated from Glycyrrhizae radix (a family of Leguminosae), is reported to have anti-bacterial activity. However, its antifungal activity is still unknown. In this present study, the antifungal activity of glycycoumarin (GLM) against Candida albicans, a polymorphic fungus was investigated. Possible mechanism such as blocking of the hyphal induction was also analyzed. By the in-vitro susceptibility analysis, GLM showed anticandidal activity, resulting in an almost complete inhibition of the fungal growth at a concentration of 320 ${\mu}g/ml$, which was equivalent to the efficacy of fluconazole at the same dose. In the murine model of disseminated candidiasis GLM enhanced resistance of mice against the disseminated disease (P<0.05), resulting in 60% protection of GLM-treated mice group during a period of 21-day observation. As for its mechanism of the antifungal activity, GLM blocked hyphal production, one of the important of virulence factors by the fungus, from the yeast form of C. albicans (P<0.01). These data indicate that GLM may contribute to the perspectives that focus on the development of a novel agent with antifungal activity specific for C. albicans infection.

Immunostimulating Components from the Root of Angelica gigas Nakai (참당귀(Angelica gigas Nakai) 뿌리의 면역증강활성 성분)

  • Ahn, Kyung-Seop;Sim, Woong-Seop;Kim, Hwan-Mook;Han, Sang-Bae;Kim, Ik-Hwan
    • Korean Journal of Pharmacognosy
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    • v.27 no.3
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    • pp.254-261
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    • 1996
  • A potent immuno-stimulating activity was detected from the watersoluble and ethanol-precipitated crude extract (AG-1) of the root of Angelica gigas Nakai. The crude extract was fractionated into two fractions, an acidic AG-2 and a neutral AG-3 fraction by DEAE-cellulose adsorption. The two fractions contained polysaccharides of which M.W. were 10 Kdal and >2,000 Kdal respectively, proteins, and various inorganic components. The immunostimulating activities of two fractions were not reduced by proteinase K, acid or alkali treatment. The polysaccharides obtained from the root of A. gigas were mainly composed of arabinose, galactose, and galacturonic acid. These results indicated that immuno- stimulating components of the root of A. gigas was a kind of pectic polysaccharides or arabinogalactans.

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Immuno-regulatory Property of Fruit-Extracts of Cornus kousa Burg. (산딸나무열매 추출물의 면역조절기능)

  • Kim, Jong-Suk;Oh, Chan-Ho;Jeon, Hoon;Lee, Kie-Seung;Ma, Sang Yong
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.5
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    • pp.327-332
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    • 2002
  • This study was conducted to investigate the immuno-regulatory effect and apoptosis of L1210 and HL60 leukemia cells of methanol-extracts of Cornus kousa Burg(CKB). The proliferation of mouse splenocytes and thymocytes enhanced by the addition of $10\;{\mu}g/ml$ of CKB. CKB were administered p.o. once a day for 7 days in adult male BALB/c mice. CKB increased the splenic and thymic T lymphocytes, especially the number of $T_H$ cells markedly increased by the treatment of CKB. CKB treatment induced the apoptotic cell death in L1210 mouse leukemia and HL60 human leukemia cells. In addition, CKB also accelerated the phagocytic activity in peritoneal macrophages and increased the production of plaque forming cells. These results suggest that CKB have an various immuno-regulatory property.

Effects of Ginsenosides Rd and Rg1 on Proliferation of B Cells and Antibody Induction (Rd와 Rg1 인삼배당체의 B 임파구 증식 및 항체 유도 효과)

  • Joo, Inkyung;Kim, Hayan;Kim, Jeonghyeon;Shehzad, Omer;Kim, Yeong Shik;Han, Yongmoon
    • YAKHAK HOEJI
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    • v.57 no.1
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    • pp.1-7
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    • 2013
  • Induction of effective and increased levels of antibody production may be major points in vaccine development. This is especially the case when the antigenic sources are carbohydrates. Thus, in our Lab various types of formulations such as liposomal and conjugate vaccines have been researched. However, the fastidious formulation process and high costs are a problem. For this reason, there is currently a focus on utilizing immunoadjuvants. In this present study, we tested whether ginsenosides Re (a panaxdiol) and Rg1 (a panaxtriol) from Panax ginseng have immunoadjuvant activity against the cell wall of Candida albicans (CACW). The resulting data showed that Rd and Rg1 caused LPS-treated B lymphocytes to be proliferative. Rd had greater proliferation activity than that of Rg1. In the murine model of antibody production, CACW combined with Rd [CACW/Rd/IFA] or Rg1 [CACW/Rg1/IFA] increased the production of antibodies specific to C. albicans when compared to the antibody production by [CACW/IFA]-induction, which was used as a negative control (P<0.05). In the case of [CFA/Rd/IFA], the antibody production was almost twice as that of the CFA. In addition, formulations containing either had a prolonged antibody inducing activity as compared to the CFA formula. In conclusion, Rd and Rg1 have an immunologic activity, and yet Rd can be a better candidate than Rg1 for a new immunoadjuvant.

Analysis of the Chemical Constituents of Agaricus brasiliensis

  • Cho, Soo-Muk;Jang, Kab-Yeul;Park, Hong-Ju;Park, Jeong-Sik
    • Mycobiology
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    • v.36 no.1
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    • pp.50-54
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    • 2008
  • This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty acids by GC were analyzed. The immuno-stimulating substance from A. blasiliensis was extracted with hot water and purified by ethanol precipitation. It underwent ion exchange chromatography on DEAE-cellulose and gel filtration on Toyopearl HW 65F. Through GP-HPLC, the substance was found to be homogeneous. Its chemical structure was determined by $^{13}C-NMR$. Fatty acids, organic acids, and sugar alcohol composition consisted exclusively of linoleic acid, fumaric acid and mannitol, respectively. The amino acids were mainly glutamic acid, glycine, and arginine. By $^{13}C-NMR$ analysis, the immuno-stimulating substance was identified as ${\beta}-(1{\rightarrow}3)\;(1{\rightarrow}6)$-glucan, composed of a backbone with $(1{\rightarrow}3)$-linked D-glucopyranosyl residues branching a $(1{\rightarrow}6)$-linked D-glucopyranosyl residue. The ${\beta}$-glucan from A. blasiliensis showed pronounced immuno-stimulating activity on the antibody-production ability of B-lymphocytes by the hemolytic suspension assay. In these results, A. blasiliensis was estimated to have potent pharmacological properties and potential nutritional values.

Experimental Effects of Acanthopanax sessiliflorus $S_{EEM}$ Extracts Following Gamma-ray Irradiation on Immuno-stimulating and anti-tumor activity in mice (방사선(放射線)이 조사(照射)된 오갈피 나무의 추출물(抽出物)이 면역기능(免役機能) 및 항암(抗癌) 기능(機能)에 미치는 실험적(實驗的) 효과(效果))

  • Kim, Hyung-Woo;Han, Jin-Geun;Kim, Geo-Woong;Go, Hong-Gae;Jeong, Hyun-Woo;Cho, Su-In
    • The Korea Journal of Herbology
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    • v.22 no.1
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    • pp.63-70
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    • 2007
  • Objectives : This experimental study was designed to investigate the effects of Acanthopanax, sessiliflorus SEEM extracts following gamma-ray irradiation on immuno-stimulating and anti-tumor activity in terms of proliferation of tumor cells, thymocytes, splenocytes, and NO production from peritoneal macrophages in mice. Methods: 10AS and 100 AS were the bark powders of Acanthopanax sessiliflorus $S_{EEM}$ stems which were exposed in 10 kGy or 100 kGy of electron beam respectively. Results : Treatment with either 10AS or 100AS increased proliferation rates of thymocytes and splenocytes significantly, and treatment with l0AS also decreased proliferation rates of tumor cells significantly. Treatment with either l0AS or l00AS promoted NO production from peritoneal macrophages significantly. Conclusion : These results suggested that AS has direct inhibition effect of tumor growth and immuno-stimulating activity. In conclusion, we demonstrate that AS could be used to treat cancer patient as complementary or alternative medicine to typical anti-cancer medication.

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Chemical Characteristics and Immuno-Stimulatory Activity of Polysaccharides from Fermented Vinegars Manufactured with Different Raw Materials (상이한 재료로 제조된 발효식초 유래 다당의 화학적 특성과 면역증진 활성)

  • Kim, Dong-Su;Hurh, Byung Serk;Shin, Kwang-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.2
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    • pp.191-199
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    • 2015
  • To elucidate the immuno-stimulatory activity of traditional fermented vinegar, six kinds of crude polysaccharides were isolated from traditional fermented vinegars manufactured with different raw materials in domestic or foreign countries, after which their chemical properties and immuno-stimulatory activities were evaluated. Of the six samples, three kinds of crude polysaccharides prepared from Korean brown rice vinegar (KBV-0), Japanese brown rice vinegar (JBV-0), and Korean persimmon vinegar (KPV-0) showed higher yields and interleukin (IL)-6 production by macrophages and were thus selected for further study. Anti-complementary activities of KBV-0, KPV-0, and JBV-0 increased dose-dependently. KBV-0 and KPV-0 showed higher anti-complementary activities ($ITCH_{50}$ 62 and 65%) than JBV-0 at $1,000{\mu}g/mL$. KBV-0, KPV-0, and JBV-0 did not affect growth of peritoneal macrophages at a dose of 1.6 to $1,000{\mu}g/mL$, where as they significantly augmented production of IL-6, IL-12, and TNF-${\alpha}$ in a dose-dependent manner. However, immuno-stimulatory activity of KPV-0 was the most potent among the tested polysaccharides. These results suggest that Korean fermented vinegars contain selected polysaccharides that confer immuno-stimulatory activities beneficial to human health.

Immuno-modulator effect of Cefodizime in IL-6

  • Joo, Seong-Soo;Oh, Won-Sik;Lee, Do-Ik
    • Proceedings of the PSK Conference
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    • pp.305.2-306
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    • 2002
  • In recent studies on cefodizime, it may potentially have the capability of stimulating chemotactic activity of neutrophils and monocytes as well as the strong immuno-modulator. In turn, infection can result in a drastic change of mediators. which lead to initiate an immune response in an indirect way. With this backgrounds, we have studied to see if cefodizime can be a potential substance to induce an immunological function in dendritic cells and peritoneal macrophages. (omitted)

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