• Title, Summary, Keyword: immunoreactive cell

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Immunohistochemical study on the gastro-entero-pancreatic(GEP) endocrine cells of the blue fox, Alopex lagopus (북극여우의 위장췌 내분비세포에 관한 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.33 no.4
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    • pp.579-589
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    • 1993
  • The regional distribution and the relative frequencies of endocrine cells were studied in nine portions of the blue fox GI tract, and the distribution pattern and cell types of the pancreativc endocrine cells were also studied in the pancreas by immunohistochemical method. Six kinds of immunoreactive cells were identified in the GI tract, and four kinds of immunoreactive cells were also identified in the pancreas. Although numerous 5-HT- and somatostatin-immunoreactive cells were seen throughout the GI tract, somatostatin- immunoreactive cells were a few in the intestine. Very numerous Gas/CCK-immunoreactive cells were restricted generally in the pyloric region and duodenum. Numerous glucagon-immunoreactive cells were found in the stomach except the pyloric region, and generally a few in the intestine. Moderate number of BPP-immunoreactive cells were found in the stomach except the pyloric region, and a few in the large intestine. Numerous porcine CG-immunoreactive cells were restricted to the cardiac and fundic region. In the pancreas, four types of pancreatic endocrine cells- somatostatin-, glucagon-, BPP- and insulin-immunoreactive- were identified in the pancreatic islet and exocrine portion. These results suggest that the regional distribution, the relative frequencies and cell types of the GEP endocrine cells in the GI tract and pancreas varies considerably among the species.

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Immunohistochemical study on the gastro-entero-pancreatic(GEP) endocrine cells of the blue fox, Alopex lagopus (북극여우의 위장췌 내분비세포에 관한 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.33 no.3
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    • pp.369-379
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    • 1993
  • The regional distribution and the relative frequencies of endocrine cells were studied in nine portions of the blue fox GI tract, and the distribution pattern and cell types of the pancreatic endocrine cells were also studied in the pancreas by immunohistochemical method. Six kinds of immunoreactive cells were identified in the GI tract, and four kinds of immunoreactive cells were also identified in the pancreas. Although numerous 5-HT- and somatostatin-immunoreactive cells were seen throughtout the GI tract, somatostatin-immunoreactive cells were a few in the intestine. Very numerous Gas/CCK-immunoreactive cells were restricted generally in the pyloric region and duodenum. Numerous glucagon-immunoreactive cells were found in the stomach except the pyloric region, and generally a few in the intestine. Moderate number of BPP-immunoreactive cells were found in the stomach except the pyloric region, and a few in the large intestine. Numerous porcine CG-immunoreactive cells were restricted to the cardiac and fundic region. In the pancreas, four types of pancreatic endocrine cells-somatostatin-, glucagon-, BPP- and insuline-immunoreactive-were identified in the pancreatic islet and exocrine portion. These results suggest that the regional distribution, the relative frequencies and cell types of the GEP endocrine cells in the GI tract and pancreas varies considerably among the species.

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An immunohistochemical study of the gut hormones in Hynobius leechii (도롱뇽의 위장관 내분비세포에 관한 면역조직화학적 연구)

  • Lee, Hyeung-sik;Lee, Jae-hyun
    • Korean Journal of Veterinary Research
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    • v.35 no.1
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    • pp.39-44
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    • 1995
  • Using immunohistochemical technique, three endocrine cell types were observed in the GIT of the Korean salamander, Hynobius leechii. BPP-immunoreactive cells were distributed from the pyloric region to the small intestine. A moderated number of somatostatin-immunoreactive cells were restricted to the pyloric region. 5-HT-immunoreactive cells were demonstrated throughout the GIT, reaching a peak in the pyloric region. The presence of BPP- and 5-HT-immunoreactive cells are identified for the first time in Hynobius leechii.

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An immunohistochemical study of the endocrine cells in gastrointestinal tract of the Korean native goat (한국 재래산양의 위장관에 출현하는 내분비세포의 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.30 no.3
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    • pp.261-270
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    • 1990
  • The gastrointestinal endocrine cells of the Korean native goat were studied immunohistochemically, and 5-HT-, somatostatin-, Gas/CCK-, glucagon-, chromogranin- and PP- immunoreactive cells were revealed. The characteristic findings of the regional distribution and relative frequency of these immunoreactive cells in the gastrointestinal tract of the Korean native goat were as follows. 5-HT-immunoreactive cells were more numerous in the small and large intestine than in the abdomen stomach. Somatostatin-immunoreactive cell were more numerous in the abdomen stomach than in the small and large intestine. Gas/CCK-immunoreactive cells were concentrated very numerously in the pyloric region with a few in the other regions. Moderate numbers of glucagon-immunoreactive cells were found in the small and large intestine, but a few of them were found in the abdomen stomach. Very numerous chromogranin-immunoreactive cells were detected throughout the gastrointestinal tract. PP-immunoreactive cells were observed moderate numbers in the large intestine with few in the ileum. No insulin-immunoreactive cell was found in the gastrointestinal tract.

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Immunohistochemistry of Glucagon- immunoreactive Cells in the Developing Pancreas of the Korean Native Goat (Capra hircus)

  • Sae-Kwang Ku;Hyeung-Sik Lee;Jae-Hyun Lee
    • Animal cells and systems
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    • v.3 no.2
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    • pp.187-191
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    • 1999
  • The distribution of glucagon-immunoreactive cells in the pancreas during various developmental stages (fetus, neonate, 1-month-old, 6-month-old and adult) of the Korean native goat was investigated by immunohistochemical methods. The varying distribution and frequency of glucagon-immunoreactive cells in the pancreas of the Korean native goat were observed. The glucagon-immunoreactive cells were detected in both exocrine and endocrine portions (pancreatic islets) at all developmental stages and also in ducts of the 6-month-old and adult. The relative frequencies of glucagon-immunoreactive cells increased in the pancreatic islets and ducts with age, but decreased in the exocrine portions. Generally, they were distributed in the interacinar spaces or marginal zone of the pancreatic islets during all stages of development. However, the cell distributions of the pancreatic islets in the neonate divided into two types: 1) ones which were distributed in the inner zone, and 2) others in the peripheral zone.

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Immunohistochemical analysis of the effect of low power GaAlAs laser treatment on the expression of proliferating cell nuclear antigen (PCNA) in full-thickness excisional wound of rat skin (CaAlAs 저출력 레이저 자극이 흰쥐의 피부 전층결손 절제 창상의 치유시 proliferating cell nuclear antigen(PCNA)발현에 대한 면역조직화학법적 분석)

  • Kim, Soon-Ja;Koo, Hee-Seo
    • Journal of Korean Physical Therapy Science
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    • v.10 no.1
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    • pp.198-205
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    • 2003
  • We evaluated the effect of low power GaAsAl laser on re-epithelization in full-thickness excisional wound of rat skin. Two full-thickness excisions were made on the back of the experimental animals. Low power laser applications with 10mW intensity were treated experimental animals twice a day for 7 days. On the seventh postoperative day the quantitative analysis of re-epithelization was performed using immunohistochemical staining for proliferating cell nuclear antigen (PCNA). The majority of PCNA immunoreactive cells was observed at epithelial cells in the margin of full thickness excisional wound. The low power laser treatments significantly increased the number of PCNA immunoreactive cell as compared to that of non treated animal group (p<0.01). The shape of PCNA immunoreactive cell appeared as small dark, round to ovoid structures. Most PCNA immunoreactive cells exhibited a high intensity of staining that contrasted sharply with the surrounding background. In conclusion, these findings suggest that GaAlAs laser treatments effectively enhance the epithelial wound healing by the stimulating cell proliferation. Furthermore, the majority of cell proliferation occurred in the margin of full thickness excisional wound.

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Immunohistochemistry of the Pancreatic Endocrine Cells of the Red-eared Slider (Trachemys scripta elegans)

  • Ku, Sae-Kwang;Lee, Hyeung-Sik;Lee, Jae-Hyun;Park, Ki-Dae
    • Animal cells and systems
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    • v.4 no.2
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    • pp.187-193
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    • 2000
  • Regional distribution and relative frequency of endocrine cells in the pancreas of the red-eared slider, Trachemys scripta elegans, were investigated by immunohistochemical methods. Chromogranin (Cg) A-, serotonin-, insulin-, glucagon-, somatostatin-, bovine pancreatic polypeptide (BPP)- and human pancreatic polypeptede (HPP)-immunoreactive cells were identified in this study. Most of immunoreactive cells in the exocrine and endocrine pancreas (Langerhans islet) were generally spherical or spindle-shaped (open-typed cell), while occasionally cells round in shape (close-typed cell) were found in the basal portion or interepithelial regions of the pancreatic duct. These immunoreactive cells were located in the exocrine, endocrine pancreas and/or basal or interepithelial portion of the pancreatic duct. Serotonin-immunoreactive cells were found in the basal portion of epithelia of the pancreatic duct at a low frequency and interacinar region of the exocrine at a moderate frequency. Insulin-immunoreactive cells were found in the central portion of the endocrine pancreas, interacinar regions of the exocrine pancreas and basal portion of the epithelia of the pancreatic duct at high, moderate and low frequencies, respectively. Glucagon-immunoreactive cells were detected in the periphery of the endocrine pancreas, interacinar region of the exocrine pancreas and basal portion of the epithelia or interepithelia of the pancreatic duct at high, moderate and moderate frequencies, respectively. Somatostatin-immunoreactive cells were dispersed in the whole area of the endocrine pancreas, interacinar regions of exocrine pancreas and basal portion of the epithelia or interepithelia of the pancreatic duct at a moderate frequency. BPP- and HPP-immunoreactive cells were detected in the iinteracinar region of the exocrine pancreas at moderate and hige frequencies, respectively. However, no Cg A- and motilin-immunoreactive cells were detected in this study.

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An immunohistochemical study of the gastro-entero endocrine cells in the snakehead, Channa(ophicephalus) argus (가물치 위장관에 있어서 내분비세포의 면역조직화학적 연구)

  • Lee, Hyeung-sik;Lee, Jae-hyun
    • Korean Journal of Veterinary Research
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    • v.36 no.2
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    • pp.297-303
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    • 1996
  • The regional distribution and relative frequency of endocrine cells in the GIT of the snakehead, Channa(Oph icephalus) argus were studied immunohistochemically. Five kinds of endocrine cells and one kind of nerve cell were identified in this study. A few numbers of secretin-immunoreactive cells were restricted to the cardia and fundus of the stomach. Moti-lin- and GRP-immunoreactive cells were rare in the epithelium of the distal and proximal intestines. PYY-immunoreactive cells were found in a few number in the pyloric caeca and were rare in the distal intestine. Substance P-immunoreactive cells were distributed relatively numerous from the fundus to the distal intetsine. Also, their nerve cells were detected in occurrence in the lamina propria in the distal intestine. No neurotensin-, met-Enk- and GIP-immunoreactive cells were found in the GIT of the snakehead.

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Differentiation and Distrbution of the Choline Acetyltransferase-immunoreactive Nerve Cells in the Magnocellular Preoptic Nucleus of the Rat Forebrains during the Postnatal Development

  • Chung, Young-Wha;Choi, Yoon-Jin
    • Animal cells and systems
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    • v.1 no.3
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    • pp.483-489
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    • 1997
  • This study was performed to investigate the differentiation and distribution of choline acetyltransferase (ChAT}-immunoreactive cells in the magnacellular preoptic nucleus (MCPO) of the postnatal and adult rat forebrains, utilizing techniques of immunocytochemistry. According to the cell shape and the ratio of long axis versus short axis of cell soma, the ChATimmunoreactive nerve cells in the MCPO were classified into six types: 1) round, 2) oval, 3) elongated, 4) fusiform, 5) triangular, and 6) polygonal types. Frequency distributions of the oval and round nerve cells on the postnatal day (PND) 0 were observed to be high. But in the adult, frequency distributions of the same cells were shown to decrease. Compared to those of the postnatal rats, frequency distributions of elongated, fusiform, triangular, and polygonal nerve cells in the adult were increased. The total mean volumes of ChAT-immunoreactive cell somata in the MCPO of PND 0 rat were the lowest, while those in the PND 17 rat were shown to be the highest and decreased in the adult. The soma volumes of the immunoreactive cells at the PND 17 were evenly distributed, but those in the other developmental stages (e.g. PND 7 and adult) appeared to exhibit unimodal distributions. On the electron micrography, the free ribosomes, polysomes, and rough endoplasmic reticula (RER) of the nerve cells in the MCPO of PND 21 rat forebrains were immunoreactive to ChAT in the tissues untreated with triton X-100. According to the observations in the present study, it is considered that the ChAT-immunoreactive nerve cells in the MCPO of the rat forebrains are differentiated throughout the following processes during the postnatal development: 1) increase in cell soma volumes, 2) development of neurites, 3) increase in the frequency of differentiated cell types, and 4) decrease in cell soma volumes. The ribosomes, polysomes, and RER are considered to be closely related to the intracellular localization and biosynthesis of the ChAT but not Golgi complex.

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Immunohistochemical studies of the pancreatic endocrine cells of the various animals (각종 동물의 췌장 내분비세포의 면역조직화학적 연구)

  • Lee, Jae-hyun;Lee, Hyeung-sik
    • Korean Journal of Veterinary Research
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    • v.32 no.4
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    • pp.497-510
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    • 1992
  • This study was attempted to comparative investigate the types and regional distribution of the endocrine cells in several vertebrates immunohistochemically using seven antisera. From carp pancreas could be observed 4 types which are insulin-, glucagon-, som- and BPP-immunoreactive cells. Insulin-immunoreactive cells were mainly distributed at the periphery and a few cells occupied the central region of the islets. Glucagon-immunoreactive cells were distributed at the periphery of the islets, and som - and BPP-immunoreactive cells were located at the central region. From frog pancreas could be observed 4 types which are insulin-, glucagon-, som- and BPP-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the islets. Som-immunoreactive cells were distributed at the periphery of the islets, and glucagon- and BPP-immunoreactive cells were found as single cell or as small groups located between the pancreatic acini. From snake pancreas could be observed 3 types which are insulin-, glucagon- and som -immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the small islets, and they also were scattered at the periphery of the large islets. Glucagon-immunoreactive cells were distributed at the periphery of the islets, whereas som-immunoreactive cells were occupied the central region. From Ogolgae pancreas could be observed 4 types which are insulin-, glucagon-, som-and BPP-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the small islets, but at the periphery of the large one. Glucagon- immunoreactive cells were distributed at the periphery of the small islets and in the large islets showed scattering entired. Som-immunoreactive cells were distributed at the periphery of the small islets and in the large islets were located at the central region. A small numbers of BPP-immunoreactive cells were located at the periphery of the small islets and the exocrine regions. From the pancreas of the Korean native goat could be observed 6 types which are insulin-, glucagon-, som-, BPP-, 5-HT- and porcine-CG-immunoreactive cells. Insulin-immunoreactive cells were distributed throughout the islets. Som-immunoreactive cells were located at the periphery of the islets, but a tew were scattered at the central region of islets and in the epithelium of the secretory duct. Glucagon-, BPP-, 5-HT- and porcine CG-immunoreactive cells were distributed at the periphery of the islets. These findings indicated that the regional distribution patterns and cell types of pancreatic endocrine cells in vertebrates varies considerably among phylogenetically different vertebrates.

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