• Title, Summary, Keyword: immunostimulating activities

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Fibrinolytic and Immunostimulating Activities of Bacillus spp. Strains Isolated from Chungkuk-jang (청국장에서 분리한 Bacillus spp. 균주의 혈전용해능 및 면역증강활성)

  • Chang, Jin-Hee;Shim, Youn-Young;Kim, Seung-Ho;Chee, Kyoo-Man;Cha, Seong-Kwan
    • Korean Journal of Food Science and Technology
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    • v.37 no.2
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    • pp.255-260
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    • 2005
  • To make Chungkuk-jang with enhanced fibrinolytic and immunostimulating activities, 220 strains isolated from Chungkuk-jangs were tested, and 13 Bacillus strains with excellent proteolytic and polysaccharide-producing activities were selected and tested for their fibrinolytic and immunostimulating activities using fibrin plate method and RAW 264.7 cell line, respectively. To assess macrophage activation, contents of cytokines such as tumor necrosis factor ($TNF-{\alpha}$) and $interleukin-1{\alpha}$ and nitric oxide were measured. Three strains showing highest fibrinolytic and immunostimulating activities were identified as Bacillus licheniformis (CHKJ 1249, 1326) and Bacillus subtilis (CHKJ 1339).

Antitumor and Immunostimulating Activities of Acanthopanax sessiliflorus Fruits

  • Lee, Sang-Hyun;Lee, Yeon-Sil;Jung, Sang-Hoon;Ji, Jun;Shin, Kuk-Hyun;Kim, Bak-Kwang;Kang, Sam-Sik
    • Natural Product Sciences
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    • v.9 no.2
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    • pp.112-116
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    • 2003
  • The antitumor and immunostimulating activities of Acanthopanax sessiliflorus fruits were investigated. Polysaccharide isolated from this plant, when administered consecutively for 9 days at 50 and 100 mg/kg i.p. in mice, caused a significant increase in the life span and a significant decrease in the tumor weight and volume in mice inoculated with Sarcoma-180 tumor cells. Polysaccharide was also demonstrated to exhibit phagocytosis-enhancing activity as measured by the carbon clearance in mice. Polysaccharide, when administered i.p. at 50 and 100 mg/kg/day for 3 consecutive days, exhibited a significant RCtr/RCc [the rate of regression coefficient of the animals teated (RCtr) to that of the control (RCc)], being 1.44 (PI = 1), 1.52 (PI = 2) which was approximately the same with that of enhancement of phagocytosis, its potency as expressed by the regression coefficient ratio of zymosan (RCtr/RCc = 1.55, PI = 2), a typical phagocytosis enhancer. Polysaccharide also caused a significant increase in the acid phosphatase activity representing lysosomal enzymes in macrophages at 1-100 ig/ml in vitro in compliance with in vivo results. These results suggest that the antitumor activity of polysaccharide might be related to the immunostimulating function.

Immunostimulating Activity of Phellinus linteus Extracts to B-lymphcyte

  • Oh, Goo-Taeg;Han, Snag-Bae;Kim, Hwan-Mook;Han, Man-Woo;Yoo, Ick-Dong
    • Archives of Pharmacal Research
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    • v.15 no.4
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    • pp.379-381
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    • 1992
  • Phellinus linteus was examined on its immunostimulating activities using an in vitro imunization and plaque forming cell assay. When lymphocytes were exposed to the extract of Phellinus linteus, the number of antibody forming cell was increased. In in vitro plaque forming cell assay, the immunostimulating effect was about 4.8 and 5.0 times of unimmunized control in polyconal and T-independent antibody response, respectively. Especially, Phellinus linteus significantly increased the antigenicity of TNP-LPS used as T-independent antigen. But Phellinus linteus did now show a mitogenic effect on B-lymphcytes. These results suggest that immunostimulating activity of Phillinus lintues might be associated with a functional stimulation of B-lympohocyte involved in humoral immune response.

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Fibrinolytic, Immunostimulating, and Cytotoxic Activities of Microbial Strains Isolated from Kochujang (고추장 분리 균주의 혈전용해능, 면역활성능 및 세포독성 효과 조사)

  • Seo, Mi-Young;Kim, Seung-Ho;Lee, Cheol-Ho;Cha, Seong-Kwan
    • Korean Journal of Food Science and Technology
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    • v.39 no.3
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    • pp.315-322
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    • 2007
  • This study was carried out to investigate the functional activities of microorganisms isolated from kochujang, such as fibrinolytic, immunostimulating, and cytotoxical activities, and to apply these microorganisms to kochujang products. Ninety-one microbial strains with proteolytic activity were selected from 294 strains isolated from traditional and commercial kochujang. Three strains (TPP 0014, TPP 6013, and TPP 6015) with high fibrinolytic activity were tested for their immunostimulating and cytotoxical activities. For the assessment of macrophage activation, cytokines such as tumor necrosis factor, $interleukin-1{\alpha}$ and nitrogen oxide were measured with the murine macrophage cell line RAW 264.7. In addition, the cytotoxical activities of the three strains were examined by MTT assay on the colon cancer cell line SNU-C4 and normal cell line CHO-K1. Using an API identifying kit, two of the microbial strains (TPP 0014 and TPP 6015) were identified as Bacillus stearothermophilus and the other strain (TPP 6013) was identified as B. amyloliquefacience.

Antitumor and Immunostimulating Activities of $Elfvingia$ $applanata$ Hot Water Extract on Sarcoma 180 Tumor-bearing ICR Mice

  • Shim, Sung-Mi;Lee, Jae-Seong;Lee, Tae-Soo;Lee, U-Youn
    • Mycobiology
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    • v.40 no.1
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    • pp.47-52
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    • 2012
  • $Elfvingia$ $applanata$, a medicinal mushroom belonging to Basidiomycota, has been used in the effort to cure cancers of the esophagus and stomach, and is also known to have inhibitory effects on hepatitis B virus infection. The hot water soluble fraction (as Fr. HW) was extracted from fruiting bodies of the mushroom. $In$ $vitro$ cytotoxicity tests showed that hot water extract was not cytotoxic against cancer cell lines such as Sarcoma 180, HT-29, HepG2, and TR at concentrations of 10-2,000 ${\mu}g/mL$. Intraperitoneal injection with Fr. HW resulted in a life prolongation effect of 45.2% in mice previously inoculated with Sarcoma 180. Treatment of Fr. HW resulted in a 2.53-fold increase in the numbers of murine spleen cells at a concentration of 50 ${\mu}g/mL$, compared with control. Incubation of murine spleen cells with Fr. HW at a concentration of 500 ${\mu}g/mL$ resulted in improved immune-potwntiating activity of B lymphocytes through an 8.3-folds increase in alkaline phosphatase activity, compared with control. Fr. HW generated 12.5 ${\mu}M$ of nitric oxide (NO) when cultured with RAW 264.7, a mouse macrophage cell line, at the concentration of 50 ${\mu}g/mL$, while lipopolysaccharide, a positive control, produced 15.2 ${\mu}M$ of NO. Therefore, the results suggested that antitumor activities of Fr. HW from $E.$ $applanata$ might, in part, be due to host mediated immunostimulating activity.

Immunostimulating Exopolysaccharide with Anticancer Activity from Enterobacter sp. SSYL[KCTC 0687BP] Screened from Ulmus parvifolia (느릅나무로부터 분리된 Enterobacter sp. SSYL[KCTC 0687P]이 생산하는 당화합물의 항암 면역활성 연구)

  • 양영렬;김영주
    • KSBB Journal
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    • v.16 no.6
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    • pp.554-561
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    • 2001
  • Immunostimulating exopolysaccharides with anticancer activity produced by Enterobacter sp. SSYL(KCTC 0687BP), isolated from Chinese elm(Ulmus sp.) were investigated. The exopolysaccharide contains molecular weight 100,000 to 1,000,000 Da and total carbohydrates 43.0 to 70.8%, total uronic acid 7.1 7o 12.4%, and total proteins 15.4 to 20.6%. Compositions and contents of sugars in the exopolysaccharides are 10-30% glucose, less than 1% fructose, 10-15% galactose, 8-12% fucose, and 40-70% glucuronic acid. The anticancer immunostimulating activities were examined and proved with regard to both in vitro and in vivo bioassays. In vivo assay, the glycoprotein at the concentration of 0.3 mg/kg showed the best result that median survival time in creased to ca. 138.1% in contrast to control groups.

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Immunostimulating and Anticancer Activities of Hot-water Extracts from Acanthopanax senticosus and Glycyrrhiza uralensis

  • Hwang, Jong-Hyun;Suh, Hyung-Joo;Yu, Kwang-Won
    • Food Science and Biotechnology
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    • v.17 no.6
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    • pp.1185-1190
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    • 2008
  • When 10 kinds of herbal medicines were fractionated into hexane, MeOH, cold-water, and hot-water extracts, hot-water extracts from Acanthopanax senticosus (AS), Glycyrrhiza uralensis (GU), Cichorium intybus (CI), and Polygonatum odoratum (PO) showed the potent intestinal immune system modulating activity (1.72-, 1.62-, 1.60-, and 1.53-fold of control at $100{\mu}g/mL$, respectively). Especially, hot-water extracts from AS (215% compared with the control) and GU (187%) also had macrophages stimulating activity and mitogenic activity of splenocytes (7.1- and 6.5-fold) at $100{\mu}g/mL$. In addition, the effects of hot-water extracts from herbal medicines on anticancer activities were studied in mice. Hot-water extracts from AS and GU enhanced cytotoxicity of natural killer cell against cancer cell, Yac-1 (37 and 34% cytotoxicity) at E/T ratio 100:1, and colon 26-M3.1 cancer cell lines had significantly inhibited (82.1 and 75.2%) in experimental lung metastasis. These results suggest that hot-water extracts from A. senticosus and G. uralensis can be used as biological response modifiers to stimulate immune system and inhibit tumor.

Immunostimulating Components from the Root of Angelica gigas Nakai (참당귀(Angelica gigas Nakai) 뿌리의 면역증강활성 성분)

  • Ahn, Kyung-Seop;Sim, Woong-Seop;Kim, Hwan-Mook;Han, Sang-Bae;Kim, Ik-Hwan
    • Korean Journal of Pharmacognosy
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    • v.27 no.3
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    • pp.254-261
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    • 1996
  • A potent immuno-stimulating activity was detected from the watersoluble and ethanol-precipitated crude extract (AG-1) of the root of Angelica gigas Nakai. The crude extract was fractionated into two fractions, an acidic AG-2 and a neutral AG-3 fraction by DEAE-cellulose adsorption. The two fractions contained polysaccharides of which M.W. were 10 Kdal and >2,000 Kdal respectively, proteins, and various inorganic components. The immunostimulating activities of two fractions were not reduced by proteinase K, acid or alkali treatment. The polysaccharides obtained from the root of A. gigas were mainly composed of arabinose, galactose, and galacturonic acid. These results indicated that immuno- stimulating components of the root of A. gigas was a kind of pectic polysaccharides or arabinogalactans.

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Study on Immunostimulating Activity of Macrophage Treated with Purified Polysaccharides from Liquid Culture and Fruiting Body of Lentinus edodes

  • Lee, Hee-Hwan;Lee, Jong-Seok;Cho, Jae-Youl;Kim, Young-Eon;Hong, Eock-Kee
    • Journal of Microbiology and Biotechnology
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    • v.19 no.6
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    • pp.566-572
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    • 2009
  • Lentinus edodes is a well-known edible and medicinal mushroom used in Oriental cultures. Recently, L. edodes has attracted a lot of attention owing to its antifungal activity, antibacterial activity, antiviral activity, hepatoprotective effect, antitumor activities, and immunomodulatory and cytotoxic effects. In this study, the water-soluble crude polysaccharides, CPF and CPB, which were obtained from the fruiting body and culture cell-free broth of L. edodes by hot-water extraction and ethanol precipitation, were fractionated by DEAE cellulose and Sepharose CL-6B column chromatography, resulting in six polysaccharide fractions, CPFN-G-I, CPFN-G-II, CPFN-G-III, CPFA-G, CPBN-G, and CPBA-G Among these fractions, CPFN-G-I, CPBN-G, and CPBA-G were shown to stimulate the functional activation of macrophages including NO production, cytokine expression, and phagocytosis.

Pharmacological Activities of a New Glycosaminoglycan, Acharan Sulfate Isolated from the Giant African Snail Achatina fulica

  • Shim, Jin-Young;Lee, Yeon-Sil;Jung, Sang-Hoon;Choi, Hyung-Seok;Shin, Kuk-Hyun;Kim, Yeong-Shik
    • Archives of Pharmacal Research
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    • v.25 no.6
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    • pp.889-894
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    • 2002
  • Acharan sulfate (AS) is a glycosaminoglycan (GAG) prepared from the giant African snail, Achatina fulica. In this study, some biological activities of AS were evaluated on the basis of structural similarities to heparin/heparan sulfate and the biological functions of GAGs. We demonstrated that it exhibited strong immunostimulating activities as measured by carbon clearance test in mice and in vivo phagocytosis. It also exhibited a significant hypoglycemic activity in epinephrine (EP)-induced hyperglycemia as well as antifatigue effects by weight-loaded forced swimming test. And it showed hypolipidemic activities in cholesterol-rich mixture induced hyperlipidemia in rats. The above results indicate that AS has diverse biological activities and suggest therapeutically important target molecules.