• Title, Summary, Keyword: ion chromatography

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Screening and Purification of Superoxide Dismutase Producing Marine Bacterium Using Photochemically Generated Superoxide Ion (광화학적으로 제조된 Superoxide Radical을 이용한 Superoxide Dismutase를 생산하는 해양미생물의 탐색 및 효소정제)

  • 조기웅
    • Korean Journal of Microbiology
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    • v.38 no.2
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    • pp.81-85
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    • 2002
  • A marine bacterium producing superoxide dismutase, strain number B446, was screened with nitrite quantitation method using hydroxy amine and photochemically generated superoxide ion, and the superoxide dismutase was purified through 35-75% ammonium sulfate precipitation, DEAE-Sephadex A-25 ion exchange chromatography, Sephadex G-200 gel filtration chromatography, and High-Q anion exchange chromatography to a yield of 6% and purification fold of 32.3.

Measurement of an Ion Concentration in Drinking Water by lon Chromatography (Ion Chromatography에 의한 음료수중 음이온 함량에 관한 연구)

  • Kim Hyung-Suk
    • Journal of environmental and Sanitary engineering
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    • v.4 no.1
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    • pp.7-15
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    • 1989
  • According to the increase of population and development of industrialization air and water pollution problems are still keeping going to great nuisance to human activities. Specially man should drink 2l clean water to maintain our health every day, but we afraid of drink the city tap water because of the contaminants like heavy metals, bacteria trihalomethane, etc. In the analysis of the anions in potable water, we usually adapt the Standard methods for the Examination of Water and Wastewater. But this method is tedious and time consuming, so the Ion Chromatography method is now used in research of water quality. Author worked with Ion Chromatography in measuring the anions in drinking water by attaching conductivity dector to normal High Performance Liquid Chromatograph. Low-capacity ion-exchange coulmn and dilute eluents, 0.00M phthalic aic was used in this study. The concentration of chloride ion was 1.55 ppm$\~$3 8.81ppm, nitrate ion was 5.45 ppm$\~$18.27ppm, and sulfate ion was 19.64 ppm$\~$28.86 ppm. The phosphate ion was detected only in Apt. tap water as 167.99 ppm whose amount was supposed to be used as a water pipe cleaner.

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Characterization of Vitamins in Yeast Extract using Gel Filtration, Ion Exchange Chromatography and HPLC (젤 여과, 이온 크로마토그래피와 HPLC에 의한 효모 엑기스내의 비타민의 분석연구)

  • 최인호;홍억기;강환구;김인호
    • KSBB Journal
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    • v.15 no.1
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    • pp.76-79
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    • 2000
  • Complex, ill-defined mixtures of natural origin are often used as nutrients in the production of biological products through microbial fermentation. Product yields are affected by variation in these natural products. Yeast extract is a typical example of these natural products. Since it is a mixture of amino acids, peptides and nucleic acids, its composition is not well characterized. In this study, we investigated the properties of thiamine hydrochloride, riboflavin and pyridoxine hydrochlride in yeast extract by using a gel filtration chromatography, ion exchange chromatography and high performance liquid chromatography. Yeast extract solution was fractionated by gel filtration chromatography and ion exchange chromatography, and then, each fraction was analyzed by using a high performance liquid chromatography.

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단백질의 치환 크로마토그래피

  • 윤태호;김인호
    • Bioindustry News
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    • v.13 no.2
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    • pp.13-17
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    • 2000
  • 새로운 분리공정으로써 크로마토그래피를 이용하고자 하는 노력들이 활발하게 진행되어지고 있는 가운데, 기존의 전통적인 크로마토그래피를 더욱 발전시킨 시도들이 행하여지고 있다. 여기에 부합하여 약 20여년 전부터 연구되기 시작한 Displacement ion-exchange chromatography는 현재 활발한 연구가 진행되어지고 있으며, 전형적인 preparative chromatography에 비해 많은 장점을 가지고 있다. Displcement ionexchange chromatography는 displacer를 사용하여, 고농도의 원료 물질을 효율적으로 단시간내에 처리할 수 있는 공정으로 기존의 preparative chromatography의 문제점이었던 꼬리끌기 현상과 희시현상을 대폭 감소시킬 수 있게 되었다. 따라서, displacement ion-exchange chromatography 공정에서의 가장 큰 과제인 비선형 등온선 영역에서의 거동과 분리공정 조작선의 결정 및 고성능의 displacer에 대한 연구가 요구되어지고 있으며, 본 총론에서는 이온교환 크로마토그래피 공정에서 displacement ion exchange chromatography를 이용한 단백질의 분리에 대하여 고찰하고자 한다.

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Chemical effect of the detergents used for cleaning a milking machine on the teatcup liner materials (착유기 세척제가 유두컵 라이너 재질에 미치는 화학적 영향)

  • Lee, Jeong-Chi
    • Korean Journal of Veterinary Service
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    • v.36 no.4
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    • pp.273-281
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    • 2013
  • This study investigated the effects of the alkaline detergent and acid rinse used for cleaning milking machines on the eight commercially available teatcup liner materials. The sample liners prepared for use in the clean-in-place process were analyzed by ultraviolet spectrophotometer, ion chromatography and liquid chromatography. Among the eight liner materials, the ultraviolet spectra of 3 sample liners were shown to have a similar peak shape after cleaning, but the ultraviolet spectra peak shape of 5 sample liners was noticeably changed. No products were detected by ion chromatography in any of the liner materials used in this study. When the liner materials were only treated with alkaline detergent, some additional peaks were observed using liquid chromatography which indicate the creation of molecular substance and elution from liner materials, however, these peaks disappeared when the liner materials were cleaned with the acid rinse. Therefore, we propose that an acid rinse should be applied, after cleaning the milking machine with the alkaline detergent.

Isolation and Purification of Polysaccharide from Fruiting body and Culture Broth of Agaricus blazei Murill

  • Youm, Yong-Soo;Hong, Eock-Kee
    • 한국생물공학회:학술대회논문집
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    • pp.343-347
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    • 2005
  • The polysaccharides were extracted from fruiting body, mycelia, and cell-free broth of Agaricus blazei Murill. The crude polysaccharides were obtained by the ethanol addtion. They were further purified using ion-exchange chromatography and gel chromatography. Ion-exchange chromatography using DEAE-cellulose column separated neutral and acidic polysaccharides. Neutral polysaccharides were then purified with gel filtration chromatography. For single peak obtained from gel filtration chromatography was molecular weight was measured with Sepharose CL-6B. The same procedure with acidic polysaccharides were performed to get the purified polysaccharides.

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Development of Capillary Columns for Ion Chromatography (이온 크로마토그래피용 모세관 컬럼의 개발)

  • Kim, Ho-Hyun;Pyo, Dong-Jin
    • Analytical Science and Technology
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    • v.12 no.6
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    • pp.521-527
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    • 1999
  • In this study, capillary columns for ion chromatography were developed to analyze trace amount of ions in samples. When small I.D. capillary columns are used in ion chromatography, the typical flow rate of the mobile phase is $5{\sim}15{\mu}L/min$ and the typical column length is 50~150 mm. The capillary columns were made using RSL-300 fused silica capillary(I.D.: 0.53 mm, O.D.: 0.67 mm) and AG14 column resin(support : polystyrene-divinylbenzene, functional group : alkyl quaternary ammonium). The new conductivity cell and suppressor were also developed and made for capillary column ion chromatography. When several anions (fluoride, nitrite, nitrate, chlorate) were analyzed using these capillary columns, reproducible and good chromatograms were obtained.

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Purification of Soybean $\beta$-Amylase (콩 $\beta$-아밀라아제의 정제에 관한 연구)

  • 안용근
    • The Korean Journal of Food And Nutrition
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    • v.7 no.1
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    • pp.23-28
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    • 1994
  • Soybean $\beta$-amylase was purified by DEAE-cellulose ion exchange chromatography, Sephadex G-100 gel chromatography, CM Sephadex C-50 ion exchange chromatography and CM Sephadex C-50 ion exchange rechromatography The purified enzyme showed 1, 020 unit/mg of specific activity. The purified enzyme was identified as homogenious by disc PAGE and analysis of reaction product.

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Determination of Halogen Elements in Volatile Organohalogen Compounds by the Wickbold Combustion Pretreatment Method and Ion Chromatography

  • Lee, Dong-Ho;Kwon, Soo-Han;Kim, Soo-Hwan;Lee, Sang-Hun;Min, Bum-Chan
    • Bulletin of the Korean Chemical Society
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    • v.28 no.1
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    • pp.59-62
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    • 2007
  • A quantitative analysis method of halogen elements in volatile organohalogen compounds was established by the Wickbold combustion pretreatment in an enclosed system and ion chromatography. The sample pretreatment parameters of Wickbold combustion were experimentally optimized, and using the determined optimal pretreatment parameters, the reproducibility was estimated by ion chromatography. As a comparison for real samples, NIST certified reference materials (CRMs) were analyzed for the recovery efficiency and reproducibility.