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AN IMMUNOHISTOCHEMICAL STUDY ON DNA SYNTHESIS OF SALIVARY GLAND TISSUE CEllS AND ENDOTHELIAL CELL AFTER IRRADIATION (방사선조사 후 타액선 세포와 혈관 내피세포의 DNA합성에 관한 면역조직학적 연구)

  • Shin Jong-Sup;You Dong-Soo
    • The Journal of Korean Academy of Maxillofacial Radiology
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    • v.21 no.2
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    • pp.183-197
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    • 1991
  • After single fraction of 2, 5, 10 Gy irradiation on submandibular gland of 40 male rats, weighing 150gm, respectively, these animal were sacrificed two hours after 0.1㎎/g bromodeoxyuridine (Sigma) peritoneal injection in 1, 3, 7, 15 hours, 1, 3, 7 days after irradiation. And excised submandibular gland were fixed in Carnoy's and Bouin's solution for 2 hours. Paraffin sections were stained with H&E, and PAS for the observation of the change of salivary gland tissue, and with Feulgen for the study of the DNA distribution, and immunohistochemically stained with anti-bromodeoxyuridine (Sanbyo Co.) for detection of DNA synthetic cells in order to study the distribution of DNA synthetic cells of salivary gland tissue and endothelium after irradiation in 5 different sites of 6 slides on X 200 high power field. The results were as followings. 1. In PAS staining 3 days after 5Gy irradiation, decreased mucine secretion of serous cells were found, and 7 days after l0Gy irradiation, decreased mucine secretion of mucous cells were found. 2. In histopathologic features, degeneration of serous cells were found in 3 days after 2 Gy irradiation and there was little change in mucous cells and excretory duct cells. 3. In Feugen staining, 3 days after 2 Gy, 5 Gy irradiation, more high percentage of DNA synthetic cells were found in intercalated duct cells, striated duct cells and excretory duct cells than in BrdU staining. 4. In immunohistochemical features, DNA synethsis of serous cells and granular convoluted tubular cells abruptly decreased in early period after irradiation and showed no recovery in 7 days after irradiation but there was an increase in DNA synthesis of intercalated duct cells, striated duct cells and excretory duct cells, which have less S-phase cells comparatively, in 7 days after 2 Gy, 5 Gy irradiation. 5. In immunohistochemical features, the DNA synthesis of endothelial cells was continuously decreased after irradiation but showed slight increase in 7 days after 2 Gy and S Gy irradiation.

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Effect of irradiation on expression of clusterin in the rat salivary glands (방사선조사가 백서 타액선의 clusterin 발현에 미치는 영향)

  • O Gyu-Myeong;Choi Yong-Suk;Hwang Eui-Hwan;Lee Sang-Rae
    • Imaging Science in Dentistry
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    • v.36 no.1
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    • pp.33-40
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    • 2006
  • Purpose : To investigate clusterin expression in the acini and ductal cells of rat submandibular glands after Co-60 gamma irradiation. Materials and Methods : The male Sprague-Dawley rats weighing approximately 250 gm were divided into control and experimental groups. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. All the rats were sacrificed at 1, 3, 7, 14, 21, and 28 days after irradiation. The specimens including the submandibular gland were sectioned and observed using a immunohistochemical method. Results : In the 2 Gy group, clusterin expression was similar to that of the control group at 1 day after irradiation and it was observed in the striated ductal cells at 3 days after irradiation. In the 5 Gy group, clusterin expression was observed in the striated ductal cells at 1 day after irradiation and gradually increased in the 10 and 15 Gy groups. In the 15 Gy group, clusterin expression was prominent in the striated ductal cells at 1 day after irradiation, but it gradually decreased with the experimental period. The destruction of the striated ductal cells was observed in the 2 Gy group at 21 days after irradiation and in the 5, 10, and 15 Gy groups at 7 days after irradiation. The destruction of the acinar cells was observed in the 2 Gy group at 28 days after irradiation and in the 5, 10, and 15 Gy groups at 14 days after irradiation. Conclusion : Clusterin expression was induced by low doses of irradiation and it appeared to be involved in the regulation of cellular response to irradiation.

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Comparison between the Effect of Intravascular Laser Irradiation Therapy with the Exterior Vascular Laser Irradiation Therapy on Hypercholesterolemia (침습, 비침습 혈관레이저에 의한 고콜레스테롤혈증의 치료효과 비교)

  • Kwon, Mi-Jung;Youn, Hyoun-Min;Kim, Young-Kyun;Kim, Won-Ill
    • Journal of Acupuncture Research
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    • v.22 no.5
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    • pp.121-132
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    • 2005
  • Objectives : The purpose of study was to compare and analyze the effect of the Intravascular Laser Irradiation Therapy and the Exterior vascular Laser Irradiation Therapy on Hypercholesterolemia. Methods : The 73 Sam-se-han-bang hospital patients who were irradiated to the Intravascular Laser and the 57 Sam-se-han-bang hospital patients who were irradiated to the Exterior vascular Laser were the study group. They had the treatment two or three times a week, and had checked total cholesterol before and after the ten times irradiation therapy. Results : Both the Intravascular Laser Irradiation Therapy and the Exterior vascular Laser Irradiation Therapy were effective on decreasing total cholesterol. The effect on decreasing total cholesterol was more definite at the abnormal group than the border line group, and the border line group than the normal group. The effect on decreasing total cholesterol was more definite at the Exterior vascular Laser Irradiation Therapy than the Intravascular Laser Irradiation Therapy, but this result was unavailable in the statistics. Conclusion : Both the Intravascular Laser Irradiation Therapy and the Exterior vascular Laser Irradiation Therapy had available effect on decreasing total cholesterol.

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An Experimental Study on the Effects of Hyperthermia and Irradiation on the Rat's Kidney (방사선조사와 온열요법이 백서신에 미치는 조직 변화에 관한 실험적 연구)

  • Yoo, Myung-Hee;Lee, Kyung-Ja;Rhee, Chung-Sik
    • Radiation Oncology Journal
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    • v.7 no.2
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    • pp.157-169
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    • 1989
  • Radiological and clinical evidences indicate that hyperthermia combined with irradiation produce a significant improvement in therapeutic effect of cancer The experiences obtained from 90 rats' kidney A single dose of irradiation ranged from 6Gy, 8Gy and 10Gy was delivered on the rat's kidney. The combined therapy group had the same irradiation after hyperthermia at $42\~44^{\circ}C$ for 30 minutes. Microscopic examination and calculation of thermal enhancement ratio were carried out, and the results were as follows: 1. In the group of hyperthermia alone, there were moderate glomerular congestion and mild tubular degeneration on light microscopic examination. 2. In the group of irradiation alone, tubular degeneration was noted in 6Gy irradiation and its severity was increased along with radiation dose. 3. In the group of hyperthermia combined with irradiation, tubular degeneration and necrosis were appeared in 6Gy and 10Gy irradiation, respectively. 4. On electron microscopic examination, proximal convoluted tubular and glomerular changes in irradiation group were similar to that of combined with hyperthermia, and its severity was increased along with observation periods. 5. Thermal enhancement ratio (TER) was 1.0 after evaluation of histipathologic changes in rat's kidney, with combination therapy.

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Wound Healing Effect of 525 nm Green LED Irradiation on Skin Wounds of Male Sprague Dawley Rats

  • Cheon, Min-Woo;Park, Yong-Pil
    • Transactions on Electrical and Electronic Materials
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    • v.11 no.5
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    • pp.226-229
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    • 2010
  • Many methods exist that promote wound healing, including light therapy, which consists of light beams that assist the human body in treating and sterilizing wounds, as well as regenerating cells. Irradiation with specific wavelengths of either laser or LED light has been shown to induce beneficial proliferation of fibroblasts that, depending on the size of the wound, can be effective in promoting wound healing. The experiments in this study utilized 8 week old 250~300 g Male Sprague Dawley Rats (ILAR Code: NTacSam:SD) and included a non-irradiation group and a 525 nm green LED irradiation group (n of each group = 7). In experiments animals were allowed to rest for 24 hours after wounds had been excised, which was followed by non- irradiation or 525 nm green LED irradiation therapy one hour per day for 9 days. Immunohistochemical staining was conducted for cytokeratin in order to precisely measure the defect size. In addition, Masson's trichrome staining was utilized in order to compare levels of collagen between the 525 nm green LED irradiation group and the non-irradiation group. Animals exposed to 525 nm green LED irradiation (p<0.05) healed at a faster rate and had increased collagenosis compared with the non-irradiated control group. Thus, treatment with 525 nm green LED irradiation had a beneficial effect on wound healing and should be considered as a possible alternative to low power laser treatment.

Analysis of Power Pattern According to Irradiation for Photovoltaic Generation System (태양광발전 시스템의 일사량에 따른 전력 패턴 분석)

  • Lee, Kyung-Sup
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.58 no.4
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    • pp.602-608
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    • 2009
  • In this thesis, output voltage, current and power of solar module were classified by irradiation from data of overall operating characteristics collected for one year in order to manage efficient photovoltaic generation system and deliver maximum power. In addition, from these data, correlations between irradiation of photovoltaic cell and amount of power given by photovoltaic cell was quantitatively examined to deduce optimization of the design and construction of photovoltaic generation system. As I-V characteristics according to a temperature range of 10~50[$^{\circ}C$], the area of I-V characteristics were increased with an increase in temperature. Since this area corresponds to the power, output power is thought to have increased with temperature. As output power characteristics according to a temperature range of 10~50[$^{\circ}C$], output power was increased with an increase in temperature. Since output power increases with temperature increase, the result corresponds well to the related equation on temperature and output power. As I-V characteristics according to a irradiation range of 100~900 [$W/m^2$], voltage and current were increased with an increase in irradiation. The result is thought of as an increase in output power with increasing irradiation. As output power characteristics according to a irradiation range of 100~900 [$W/m^2$], output power was increased with increasing irradiation. This result corresponds well to the related equation on irradiation and output power.

The Characteristics of Persimmon Juice Dyeing using Padding and UV Irradiation Method (PartII) - Color and Properties of Persimmon Juice Dyed Silk Fabrics - (패딩과 자외선조사법을 이용한 감즙 염색 특성(제2보) -감즙 염색 견직물의 염색성과 물질-)

  • 이혜자;한영숙
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.7
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    • pp.882-891
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    • 2004
  • The purpose of this study was to improve dye effect and method in order to facilitate the use of persimmon juice dyeing. Silk fabrics were pad-dyed to 100% pick-up using padding machine after dipping in persimmon juice extracted from unripe persimmons indigenous to Jeju. The color of dyed silk fabrics by padding method was more even and repeatable than that by traditional hand method. Persimmon juice concentrations were 4 types 10, 25, 50 and 100%. The silk fabrics were pad-dyed to 100% pick-up and were repeatedly dyed two times and three times with 100% concentration. The higher the concentration, the more color deepened. UV Irradiation instead of sunlight was applied to color developing. Irradiation times were shortened to 1-8hrs. Color values which reguired 30-50 hrs. in sunlight irradiation. were obtained in 3-5 hrs. with UV irradiation. Tensile strengths of silk fabrics in UV irradiation decreased but not decreased in sunlight irradiation. Elongations of silk fabrics in sunlight irradiation increased but decreased in W irradiation. Drape stiffness increased up to three times.

Use of Ionizing Radiation as a Phytosanitary Treatment for Postharvest Disease Control

  • Jeong, Rae-Dong
    • Journal of Radiation Industry
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    • v.8 no.2
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    • pp.97-104
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    • 2014
  • Postharvest diseases cause considerable losses to harvested fruits and vegetables worldwide. Fresh produce suspected of harboring postharvest disease must be treated to control any pathogens present. Although there are various treatments to control postharvest losses by pathogens, the current community is eager to take safer and more eco-friendly alternatives to help with human health and reduce environmental risks. Ionizing irradiation is a promising phytosanitary treatment that has a significant potential to control postharvest diseases in use worldwide. Although almost 19000 metric tons of sweet potatoes and various fruits are irradiated each year in six countries to control postharvest disease, irradiation continues to be a debate, with slow acceptance by industries. Irradiation alone is not effective as a fungicide, and an over dose affects the physical properties of irradiated products. A combination of irradiation with other treatments such as heating, biocontrol agents, chlorination, and nano Ag particles is to enhance their effectiveness. Challenges to the use of phytosanitary irradiation are an avoidance of irradiated postharvest and cost of the irradiation facilities, and thus consumers still need to be educated on the principles and benefits of irradiation and prepare an optimum economy of scale for commercial use. In this review, we evaluated the current phytosanitary irradiation, and combination with various other treatments to minimize the postharvest losses.