• Title, Summary, Keyword: karyotype

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Karyotype of Dwarf Loach, Kichulchoia brevifasciata (Pisces: Cobitidae) from Korea (미꾸리과 소형어류 좀수수치, Kichulchoia brevifasciata의 핵형)

  • Kim, Ik-Soo;Kim, Eun-Jin
    • Korean Journal of Ichthyology
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    • v.20 no.1
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    • pp.61-65
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    • 2008
  • The karyotype analysis of Kichulchoia brevifasciata was carried out, known as an end-angered small endemic cobitid fish of Korea. Five females and one male were collected from Geum-san-myeon, Goheung-gun, Jeollanam-do, Korea and its gill, kidney and digestive tract tissues were treated by flame-drying technique. The karyotype was found to be 2n= 48 from 33 cells; 16 meta-submetacentric and 32 subtelo-telocentric chromosomes; 64 FN (fundamental number). This karyotype showed a remarkably different karyotype formula from Niwaella multifasciata which is closely related to this species.

Influence of heat shock, drugs, and radiation on karyotype of Leishmania major (열대리슈마니아 핵형에 대한 열쇽, 약제 및 감마선 조사의 영향)

  • Min Seo;Duk-Kyu Chun;Sung-Tae HONG;Soon-Hyung Lee
    • The Korean Journal of Parasitology
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    • v.31 no.3
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    • pp.277-284
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    • 1993
  • Leishmaniasis is one of the important tropical diseases in the world. Although it is not prevalent In Korea, imported cases have been recorded. The karyotype of Leishmcnic sp. has been observed to be variable by localities or by strains, but the karyotype of a strain is known to be stable. This study was performed to observe if the karyotype of a Leishmonio sp. would be changed under some stressful conditions. The karyotype, analyzed by pulsed Held gradient gel electrophoresis, was not grossly changed by heat shock, chemotherapeutics, UV illumination, and gamma irradiation. Radiation destroyed the chromosomes mechanically but subcultured organisms after irradiation showed unaffected karyotype. The present findings suggest that the karyotype of a Leishmnnia strain is so stable that it is not altered by temporary stimulation with heat, drugs, and radiation.

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Karyotype Analysis of Eight Korean Native Species in the Genus Iris

  • Kim, Hyun-Hee;Park, Young-Wook;Yoon, Pyung-Sub;Choi, Hae-Woon;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.5
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    • pp.401-405
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    • 2004
  • Karyotypes were established in the eight Korean native species of the genus Iris. Chromosome numbers were 2n=50 in I. koreana and 2n=42 in I. uniflora var. carinata and their karyotype formulas were K = 2n = 50 = 14m + 28sm + 8st and K = 2n = 42 = 16m + 26sm, respectively. I. dichotoma and I. pseudoacorus were diploids of 2n=34. However, they showed different karyotype formulas: K = 2n = 34 = 26m + 6sm + 2st in I. dichotoma and K = 2n = 34 = 8m + 24sm + 2st in I. pseudoacorus. I. setosa, and I. pallasii var. chinensis carried the same chromosome numbers of 2n=40, but they showed different patterns of karyotype formula: K = 2n = 40 = 22m + 14sm + 4st in I. setosa and K = 2n = 40 = 26m + 12sm + 2st in I. pallasii var. chinensis. I. sanguinea was a diploid of 2n=28 and the karyotype formula was K = 2n = 28 = 14m + 14sm. I. ensata var. spontanea was a diploid of 2n=24 and the karyotype formula was K = 2n = 24 = 10m + 14sm. Each species showed characteristic chromosome composition with a pair of satellite chromosome except I. koreana with three pairs of satellite chromosomes. The chromosomes of I. dichotoma and I. uniflora were comparatively short, while the chromosomes of I. ensata were remarkably bigger than those of other species. These cytological data will give a useful information for the identification and breeding program of the Iris plants.

Computing of the Fuzzy Membership Function for Karyotype Classification (핵형 분류를 위한 퍼지 멤버쉽 함수의 처리)

  • Eom, Sang-Hee;Nam, Jae-Hyun
    • Journal of the Korea Society of Computer and Information
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    • v.11 no.6
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    • pp.1-8
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    • 2006
  • Many researchers have been studied for the automatic chromosome karyotype classification and analysis. For the automatic classify the each chromosome which is the image in microscope, it is necessary to process the sub-procedure, ie. image pre-processing, implementing karyotype classifier. The image pre-processing proceeded the each chromosome separation, the noise exception and the feature parameter extraction. The extracted morphological feature parameter were the centromeric index(C.I.), the relative length ratio(R.L.), and the relative area ratio(R.A.). In this paper, the fuzzy classifier was implemented for the human chromosome karyotype classification. The extracted morphological feature parameter were used in the input parameter of fuzzy classifier. We studied about the selection of the membership function for the optimal fuzzy classifier in each chromosome groups.

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Electrophoretic Karyotyping by PFGE in the Genus Fusarium (Fusarium속에서 PFGE를 이용한 Electrophoretic Karyotyping)

  • Min, Byung-Re;Jung, Jin-Sook;Choi, Yong-Keel
    • The Korean Journal of Mycology
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    • v.26 no.2
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    • pp.135-143
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    • 1998
  • Contour-clamped homogeneous electric field gel electrophoresis was used to establish electrophoretic karyotype for 10 species of Fusarium sections Sporotrichiella, Liseola, Gibbosum, Discolor and Martiella. Intact chromosomal DNA was isolated from fungal protoplast and separated under various conditions according to their size in order to improve DNA separation. The numbers of chromosome-sized DNA molecules for individual species ranged from 5-13, with individual chromosomes ranging from 0.78 Mb to 7.20 Mb in size. The total genome DNA size of each species was estimated at about 18.32 Mb to 48.20 Mb. Comparison of karyotype profiles following Southern hybridization analysis with a randomly selected genomic probe of F. oxysporum formae speciales litii was carried out.

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Esthesioneuroblastoma in a boy with 47, XYY karyotype

  • Jo, Hee Cheol;Lee, Seong Wook;Jung, Hyun Joo;Park, Jun Eun
    • Clinical and Experimental Pediatrics
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    • v.59 no.sup1
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    • pp.92-95
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    • 2016
  • Neuroblastomas are sometimes associated with abnormal constitutional karyotypes, but the XYY karyotype has been rarely described in neuroblastomas. Here, we report a case of an esthesioneuroblastoma in a boy with a 47, XYY karyotype. A 6-year-old boy was admitted to our hospital because of nasal obstruction and palpable cervical lymph node, which he first noticed several days previously. A polypoid mass in the right nasal cavity was detected through sinuscopy. Biopsy of the right nasal polyp was performed. Based on the result, the patient was diagnosed with a high-grade esthesioneuroblastoma. Nuclear imaging revealed increased uptake in both the right posterior nasal cavity and the right cervical IB-II space, suggesting metastatic lymph nodes. Cytogenetic analysis revealed a 47, XYY karyotype. Twelve courses of concurrent chemotherapy were administered. Three years after the completion of chemotherapy, the patient had had no disease recurrence. He manifested behavioral violence and temper tantrums, so we started methylphenidate for correction of the behavior.

The Karyotype of a Riceash, Oryzias latipes from Southern Korea (한국 남부지방에 서식하는 송사리 Oryzias latipes (Temminck et Schlegel)의 핵형)

  • 김익수;문교정
    • The Korean Journal of Zoology
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    • v.30 no.4
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    • pp.379-386
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    • 1987
  • Karyotype of the ricessh Orygias latipes (Temminck et Schlegel) was investigated using 151 specimens of eleven populations in the southern Korea. Based on the karyotype analysis, two distinct chromosomal groups were found. One group, occurring in the Kum River, the Mankyong River, the Dongjin River, the Ysngsan River and the SEmjin River had a diploid chromosome number of 46 and arm number of 70, which contained one pair of "large" metacentric chromosomes. The other group, occurring in the Tamjin River, the Hyongsan River andthe all four island populations observed had a diploid chromosome number of 48 and arm number of 68. These results showed that 0. latipes of 6 inland populations was identical with the Chinese population in diploid number, arm number and presence of "large" metacentric chromosome, while the present species of island populations ana populations of the Tamjn R. and Hyongsan R. was similar to the Japanese population in chromosome number and arm number. The karyotype variation in populations indicated that karyotypes will probably provide useful information in studying zoogeography of this species.phy of this species.

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Karyotype Analysis of Tobacco Moth, Ephestia elutezza H. (Lepidoptera : Pyralidae) and Cigarette Beetle, Lasioderma serricorne F. (Coleoptera : Anobiidae) (다색알락명나방(Ephestia elutella H.)과 궐련벌레(Lasioderma serricorne F.)의 핵형 분석)

  • Chae, Soon-Yong;Kim, Sang-Seock;Chang, Young-Dock
    • Journal of the Korean Society of Tobacco Science
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    • v.18 no.1
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    • pp.30-38
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    • 1996
  • Meiotic metaphase chromosomes of the testis of two storage insects, tobacco moth (Ephestia elutella H.) and cigarette beetle (Lasioderma serricorne F.) were examined to study their karyotypes. The number of haploid chromosomes of the tobacco moth was 31 and the karyotype was characterized by 29 metacentric and 2 submetacentric chromosomes. The metaphase chromosome length of tobacco moth ranged ca. 1.44-2.11${\mu}{\textrm}{m}$ and the average length was 1, 78${\mu}{\textrm}{m}$. The number of haploid chromosomes of the cigarette beetle at metaphase was 10 and the karyotype was characterized by 8 metacentric and 2 submetacentric chromosomes. The range of metaphase chromosome length of the cigarette beetle was ca. 1.79- 2.39${\mu}{\textrm}{m}$ and the average length 2.09${\mu}{\textrm}{m}$.

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Karyotype and Insecticide Resistance of the Green Peach Aphids (Myzus persicae Sulzer) collected from Tobacco fields (연초포장에서 발생하는 복숭아혹진딧물의 핵형과 살충제 저항성)

  • 채순용;김영호;김상석;장영덕
    • Journal of the Korean Society of Tobacco Science
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    • v.19 no.2
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    • pp.107-116
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    • 1997
  • Karyotype of apterous green peach aphid (Myzus persicae SuIBer) populations occurring in tobacco fields and their resistance to insecticides were investigated. All of the 16 clones collected from tobacco fields had same chromosome number of 2n=12. Among them, 5 clones had the normal karyotype, regardless of color morph, but chromosomal translnations between #1 and #3 autosomal chromosomes were noted in the other 11 clones. All of the aphid clones were highly resistant tolambda cyhalothrin, a pyrethroid pesticide, having over 20 times higher concentrations of LD90 than recommended concentration (10ppm) for the aphid. However, their resistance to acephate, demeton 5-methyl and pirimicarh varied depending on the clones, among which green-colored clones were generally more resistant to the pesticides than red-colored ones. No association was found between the insecticide resistance and the karyotype of the aphid in this experiment.

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Speciation Mode Reconstruction for Lepilemur six species (L. mustelinus, L. edwardsi, L. dorsalis, L. leucopus, L. ruficaudatus, L. septentrionalis) Based on the Lepilemur Karyotype Analysis (여우원숭이속(Lepilemuridae)의 핵형 분석을 통해 나타난 Lepilemur 6종(L. mustelinus, L. edwardsi, L. dorsalis, L. leucopus, L. ruficaudatus, L. septentrionalis)의 종 분화 양상)

  • 정기윤
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.5 no.2
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    • pp.141-149
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    • 2004
  • The aim of this study was to test the validity of the hypothesis that the karyotypes of four species of Lepilemuridae were formed spontaneously from their ancestral hybrid karyotype. Hypothetical ancestral haploid Karyotype of Lepilemuridae is composed of 18 autosomes and X chromosomes. Lepilemur mustelinus karyotype has four tandem fused chromosomes and one Robertsonian translocated chromosome pairs. Lepilemur septentrionalis septentrionalis karyotype has only two pairs of translocated chromosomes. We reconstruct and suggest ancestral karyotype of LMU(ancLMU) and LSS(ancLSS), from which all four studied species were derived. Hybrids of ancLMU and ancLSS were formed and produce differently fused equilibrated gametes via circular form arrangement during gametogenesis. Five unit of trivalent homologous chromosome pairs were engaged in a circular form to give new gamete corresponding to the karyotype of L. dorsalis, orientation of one unit of trivalent was inversed in the circle to gave new gamete corresponding to the karyotype of L. leucopus. Seven homologous chromosome pairs were engaged in circular form to give haploid karyotype of Lepilemur ruficaudatus. Only one homologous chromosome pair is dissociated and the other chromosome pairs rearranged in the circle to form haploid karyotype of Lepilemur edwardsi. The new gametes could be produced from these circular forms. When the new gamete fertilized with the same type of gamete, The new homozygote is produced as existing L. dorsalis, L. leucopus, L. edwardsi and L. ruficaudatus. These results support the theory that new species could be formed in hybrid population through activated chromosome fusion, chromosome rearrangement in circular from at zygotene stage and production of equilibrated gametes to form homozygote new species.

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