• Title/Summary/Keyword: large intestinal bacteria

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Effect of Kimchi Intake on the Composition of Human Large Intestinal Bacteria (김치의 섭취가 인체의 장내 미생물에 미치는 영향)

  • Lee, Ki-Eun;Choi, Un-Ho;Ji, Geun-Eog
    • Korean Journal of Food Science and Technology
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    • v.28 no.5
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    • pp.981-986
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    • 1996
  • We have conducted this study to examine effect of kimchi intake on the composition of human large intestinal bacteria. Two hundred grams of kimchi were administered to 10 healthy young volunteers (20-30 years old) every day for 2 weeks, followed by 2 weeks of non-intake period. The non-intake-intake cycle was repeated for 10 weeks. Except antibiotics and materials which contain live bacteria, subjects were allowed to eat ad libitum. The composition of intestinal microflora (Bacteroides, Bifidobacterium, Escherichia coli, Streptococcus, Lactobacillus, Leuconostoc, Staphylococcus, Clostridium perfringens) was examined a1 the last day of each period. $\beta-Glucosidase$ and $\beta-glucuronidase$ activities, pH and moisture content of the fecal samples were also measured. During the administration of kimchi, the cell counts of Lactobacillus and Leuconostoc increased significantly (p<0.05), whereas those of other bacteria did not change significantly. The enzyme level of $\beta-glucosidase$ and $\beta-glucuronidase$ decreased during kimchi intake (p<0.05). Results indicate that a portion of lactic acid bacteria present in kimchi can pass human stomach and reside in the large intestinal tract.

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Comparison of Cultured Soymilk by Bifidobacterium and Various Human Intestinal Bacteria (Bifidobacterium과 기타 장내 세균에 의한 두유 배양 비교)

  • Lee, Se-Kyung;Son, Heon-Soo;Ji, Geun-Eog
    • Korean Journal of Food Science and Technology
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    • v.25 no.6
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    • pp.694-697
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    • 1993
  • Soymilk was cultured by various human large intestinal bacteria and lactic acid bacteria; Bifidobacterium longum, Lactobacillus acidophilus, Baeteroides fragilis, Eubacterium limosum, Clostridium perfringens and Escherichia coli. Among them, only B. longum utilized raffinose and stachyose actively which are major oligosaccharides present in soymilk by producing active ${\alpha}-galactosidase$ and produced greatest acid. Number of colony forming unit of B. longum reached $1.5{\times}10^{8}$ after 16 hr culture in soymilk. Also Bifidobacterium longum produced the highest level of ${\alpha}-galactosidase,\;{\beta}-galactosidase\;and\;{\alpha}-galactosidase$, in soymilk during culture.

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Investigation of Selective Medium for Isolation and Enumeration of Bacteroides sp. from the Feces of the Korean People (한국인의 분변으로 부터 Bacteroides를 분리하기 위한 선택 배지 조사)

  • Ji, Geun-Eog;Kim, In-Hee;Lee, Se-Kyung
    • Korean Journal of Food Science and Technology
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    • v.26 no.3
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    • pp.295-299
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    • 1994
  • Vancomycin inhibited the growth of the most large intestinal bacteria other than Bacteroides sp. and E. coli. Since Bacteroides are predominant compared with E. coli in the large intestinal tract of the adult, vancomycin-added medium(VA) was shown to be an effective selective medium for Bacteroides. VA showed comparable or better selectivity for Bacteroides sp. to NBGT which is being used currently as a most frequently used selective medium for Bacteroides in Japan.

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