• Title, Summary, Keyword: liquid chromatography

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Microcystin Detection Characteristics of Fluorescence Immunochromatography and High Performance Liquid Chromatography

  • Pyo, Dong-Jin;Park, Geun-Young;Choi, Jong-Chon;Oh, Chang-Suk
    • Bulletin of the Korean Chemical Society
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    • v.26 no.2
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    • pp.268-272
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    • 2005
  • Different detection characteristics of fluorescence immunochromatography method and high performance liquid chromatography (HPLC) method for the analysis of cyanobacterial toxins were studied. In particular, low and high limits of detection, detection time and reproducibility and detectable microcystin species were compared when fluorescence immunochromatography method and high performance liquid chromatography method were applied for the detection of microcystin (MC), a cyclic peptide toxin of the freshwater cyanobacterium Microcystis aeruginosa. A Fluorescence immunochromatography assay system has the unique advantages of short detection time and low detection limit, and high performance liquid chromatography detection method has the strong advantage of individual quantifications of several species of microcystins.

High Purity Extraction and Simultaneous High-performance Liquid Chromatography Analysis of Curcuminoids in Turmeric (강황에서 curcuminoids의 고순도 추출 및 고성능 액체 크로마토그래피 동시분석)

  • Lee, Kwang-Jin;Ma, Jin-Yeul;Kim, Young-Sik;Kim, Dong-Seon;Jin, Yinzhe
    • Journal of Applied Biological Chemistry
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    • v.55 no.1
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    • pp.61-65
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    • 2012
  • Three major curcuminoids in turmeric (Curcuma longa), curcumin, demethoxycurcumin, and bisdemethoxycurcumin, were efficiently extracted by optimizing extraction condition and simultaneously analyzed and identified using reverse phase high-performance liquid chromatography, thin-layer chromatography, and liquid chromatography-mass spectrometry method. The highest yield of extraction amount 0.279 g, 9.30% was obtained by dipping method with extraction time of 7 h.

Separation Characteristics of IgY (Immunoglobulin Yolk) in Various HPLC Columns (다양한 HPLC Column에서의 IgY(Immunoglobulin Yolk) 분리특성)

  • Song, Sung Moon;Kim, In Ho
    • Korean Chemical Engineering Research
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    • v.50 no.4
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    • pp.659-665
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    • 2012
  • IgY (Immunoglobulin Yolk) in egg yolk corresponds to IgG (Immunoglobulin G) in animal serum and plays an important role as immunological proteins in intestines. Carrageenan and Arabic gum were used as pretreatment agents to purify IgY from fresh egg yolk. DEAE (Diethylaminoethyl) Sepharose column in FPLC (Fast Protein Liquid chromatography) was an ion exchange tool to remove contaminants as well as to elute IgY from the column. GF HPLC (Gel Filtration High Performance Liquid Chromatography) enables to measure the molecular weights of IgY and to identify the purified IgY by comparing the molecular weight of standard IgY with the purified one. IgY is a heterogeneous group of different molecular weight and ionic properties, which was investigated with various IE HPLC (Ion Exchange High Performance Liquid Chromatography) columns such as AX, CX and SCX. Three peaks of IgY were separated in the AX column under the conditions of 0.5 M NaCl and pH=8. The SCX column also gave the three peaks of IgY at 0.5 M NaCl and pH=5.

Chemical effect of the detergents used for cleaning a milking machine on the teatcup liner materials (착유기 세척제가 유두컵 라이너 재질에 미치는 화학적 영향)

  • Lee, Jeong-Chi
    • Korean Journal of Veterinary Service
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    • v.36 no.4
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    • pp.273-281
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    • 2013
  • This study investigated the effects of the alkaline detergent and acid rinse used for cleaning milking machines on the eight commercially available teatcup liner materials. The sample liners prepared for use in the clean-in-place process were analyzed by ultraviolet spectrophotometer, ion chromatography and liquid chromatography. Among the eight liner materials, the ultraviolet spectra of 3 sample liners were shown to have a similar peak shape after cleaning, but the ultraviolet spectra peak shape of 5 sample liners was noticeably changed. No products were detected by ion chromatography in any of the liner materials used in this study. When the liner materials were only treated with alkaline detergent, some additional peaks were observed using liquid chromatography which indicate the creation of molecular substance and elution from liner materials, however, these peaks disappeared when the liner materials were cleaned with the acid rinse. Therefore, we propose that an acid rinse should be applied, after cleaning the milking machine with the alkaline detergent.

Mutation Analysis in β2-Adrenergic Receptor Gene by Denaturing High Performance Liquid Chromatography (DHPLC) (DHPLC를 이용한 β2-교감신경수용체 유전자에서의 돌연변이 분석)

  • Park, Sang-Bum;Oh, Chung-Hun;Kim, Jong-Wan;Jang, Won-Cheoul
    • Analytical Science and Technology
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    • v.15 no.3
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    • pp.190-195
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    • 2002
  • We analysed mutation of ${\beta}_2$-adrenergic receptor gene that controls bronchial asthma by denaturing high performance liquid chromatography (DHPLC) according to ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC). We extracted genomic DNA from 50 asthma patients, amplified DNA using PCR, and analysed PCR product by DHPLC. As a result, we obtained that mutation frequency was 15 (30%) among 50 cases. Consequently DHPLC mutation detection was confirmed that the result of direct sequencing was coincide exactly.

Hydrolysis kinetics of Metampicillin by High Performance Liquid Chromatography

  • Lee, Hee-Yong;Jang, Won-Cheoul;Lee, Hye-Suk
    • Archives of Pharmacal Research
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    • v.17 no.5
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    • pp.378-380
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    • 1994
  • The hydrolysis of metampicillin to ampicillin was investigated using high perofrmance liquid chromatography. We developed the simultaneous determination of metampicillin and ampicilin using a Zorbox CN column and 5% acetonitrile and 8% methanol in 0.02 M phosphate buffer (pH 7.0) as mobile phase. Matampicillin was hdyrolyzed to ampicillin with half life of 41.5 min at physiological pH and temperature. In acdic pH, metampicillin was rapidly hydrolyzed to ampicillin within a chromatogrphic separation.

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Analysis of Benzophenone in Sediment and Soil by Gas Chromatography/Mass Spectrometry (기체크로마토그래피/질량분석기에 의한 저질 및 토양시료 중 벤조페논의 분석법 연구)

  • 권오승;김은영;류재천
    • Environmental Analysis Health and Toxicology
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    • v.16 no.3
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    • pp.121-126
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    • 2001
  • Analytical method of benzophenone (BP) in sediment and soil was developed by gas chromatography/mass selective detector/selected ion monitoring (GC/MSD/SIM). The ultrasonic extraction of US EPA (method 3550B) method and liquid-liquid extraction for sediment and soil samples were used for the analysis of BP from sediment and soil. BP was extracted with n-hexane. Organic layer was washed with 5% sodium chloride solution. 1∼2 l of the concentrated solution of organic layer was applied to GC/MSD. The retention time of BP peak was 11.10 min. Recovery (%) of BP by ultrasonication from sediment and soil samples was 96.0∼100.6% and 40.0∼83.0%, respectively. Recovery of BP by liquid-liquid extraction was 51∼59% in soil samples. The detection limit of BP in sediment and soil samples were determined to 0.1 ng/g.

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Isolation of ginsenosides Rb1, Rb2, Rc Rd, Re, Rf and Rg1 from cinseng root by high performance liquid chromatography

  • Paik, Nam-Ho;Park, Man-Ki;Choi, Kang-Ju;Cho, Yung-Hyun
    • Archives of Pharmacal Research
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    • v.5 no.1
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    • pp.7-12
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    • 1982
  • Ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf and Rg1 were effectively isolated from ginseng root by preparative liquid chromatography (LC) on two PrepPAK-500/c18 cartridges in series and semipreparative LC on a .mu. Bondapak cabohydrate analysis column, a .mu.Bondapak C18 column or a .mu. Porasil column. The identities of the isolated ginsenosides were confirmed by analytical high-performance liquid chromatography (HPLC) and infrared spectrophotometry. By this method large scale isolation of pure ginsenosides was efficiently accomplished.

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Detectio of Malonaldehyde-thiobarbituric Acid (MA-TBA) Complex by High Performance Liquid Chromatography(HPLC) in a Model System

  • Whang, Key
    • Preventive Nutrition and Food Science
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    • v.4 no.3
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    • pp.167-170
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    • 1999
  • Various concentrations of malonaldehyde (MA) produced upon hydrolysis of 1, 1, 3,3-tetraethoxypropane (TEP) were reacted with 2-thiobarbituric acid (TBA)and th e contents of MA-TBA complex were measured both with spectrophotometer and high performance liquid chromatography (HPLC). As the concentrations of MA-TBA increased, their absorbances and the corresponding HPLC peak areas increased. The correlation coefficient between absorbances and HPLC peak areas of MA-TBA peaks from the other compounds and butanol extraction of the complex increased its recovery its recovery by 29.4% . Measurement of the content of MA-TBA complex for monitoring the development of lipid oxidation was proven to be successful with the use of high performance liquid chromatography.

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