• Title, Summary, Keyword: liver morphology

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Effects of Alcohol Consumption and Fat Content in Diet on Chemical Composition and Morphology of Liver in Rat (알콜과 식이지방량이 흰쥐의 간 지질조성과 간조직형태에 미치는 영향)

  • 정경희
    • Journal of Nutrition and Health
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    • v.21 no.3
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    • pp.154-163
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    • 1988
  • Effects of alchohol and fat content in a balanced diet on chemical composition and morphology of liver were investigated in growing rats. Fourth eight male rats of Sprague-Dawley strain weighing about 160g were divided into 4 groups ; high fat diet group, alcohol-administered high fat diet group, low fat diet group and alcohol-administered high fat diet group, low fat diet group and alcohol-administered low fat diet group. High and low fat diets supplied 30% and 12%, respectively, of total calorie intake from fat, and alcohol was given by adding ethanol in drinking waster at 10%. Diets contained adequate amounts of all nutrients required for rats, including lipotrpoic agents(choline and methionine) to minimize effects of factors other than alcohol on liver damage. Ratios of liver weight to body weight were statistically different among groups. Liver/dody weight ratios alcohol-administered rats were significantly higher than those of non-alcohol groups after 6 weeks treatment. Although total lipid and triglyceride per gram liver were increased in alcohol-administered rats, especially low fat diet fed rats, the values were not significantly different. Opticmicroscopical observation revealed increase in cell size and no change in morphology of liver. Examination of hepatocytes by electron microscopy showed that fat droplets were observed in all groups but enlarged in the alcohol-administered low fat diet fed rat. Contents of protein, cholesterol and phospholipid were not affected by alcohol consumption. The level of lipid peroxide was significantly lower in the livers of alcohol-administered rats than in the livers of non-alcohol groups. The results of this study indicate that even moderate alcohol drinking and dietary fat content did not affect any significant change in composition and morphology of liver until 6 week treatment but that even moderate alcohol drinking caused some signs of steatosis of liver.

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Changes on function and morphology of liver in carcinogen-induced hepatoma rats (II. Changes on morphology of liver) (발암제에 의해 간종양이 유발된 쥐에서 간기능과 형태학적 변화에 관한 연구 (III. 간의 형태학적 변화에 관하여))

  • 김철호;문평일;강정부
    • Korean Journal of Veterinary Service
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    • v.22 no.2
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    • pp.135-149
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    • 1999
  • This study was concerned with assessment of dietynitrosamine (DEN) induced liver cell carcinogenesis by measurement of changes preceding the development of neoplasms. The changes of hepatic morphology in rats(Sprague-Dawley) were detected by hematoxylin-eosin stain and immunohistochemistry(PCNA). The results were as follows ; 1. Minor behavioral change, brittleness of hair and decreased amount of water and diet intake. were observed in rats 7 weeks after DEN administration. 2. Variable size of liver tumor and hepatomegaly were observed in rats treated with DEN after 10 weeks. 3. Numerous vacuoles were showed on the midzonal and or peripheral areas of hepatic lobules. The large and polymorphological hepatocytes with eosinophilic cytoplasm or densely basophilic mitotic nucleoli were showed. 4. Several proliferative small round cells were shown on vacuolated and necrotic areas in peripheral hepatic lobules or portal areas. 5. PCNA-positive cells were showed on the vacuolated portal areas and peripheral areas of hepatic lobules. Maximal positivity was 23.6% in the areas of small round cells. In conclusion, this result confirmed that the DEN was one of the potent hepatocarcinogens. In histopathological analysis, the altered foci, hyperplastic nodules, neoplastic nodules, adenomas and carcinomas were observed in liver tumors induced by administration of DEN in rats.

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Effects of Pine Needle Extracts on Enzyme Activities of Serum and Liver, and Liver Morphology in Rats Fed High Fat Diet (솔잎추출물이 고지방식이를 급여한 흰쥐의 혈청, 간장의 효소 및 간조직구조에 미치는 영향)

  • 박용곤;강윤한;하태열;문광덕
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.3
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    • pp.374-378
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    • 1996
  • The effects of pine needle extracts on lipid contents and antioxidative enzyme activities in liver of rat were evaluated. Thirty male Sprague-Dawley rats were divided into five groups and fed high fat diets for four weeks. Each group was administered with pine needle extract in the following doses: control, water ; WE-3, hot water extract(3% PN) ; WE-6, hot water extract(6% PN) ; AE-3, acetone extract(3% PN) ; AE-6, acetone extract(6% PN). The results obtained from the experiment were as follows: GOT activities were not significantly different among experimental groups but GPT activities were significantly low in the experimental groups compared to the control group. Liver superoxide dismutase(SOD) activity of pine needle extract administered groups was higher than that of control group. Catalase activities of liver had a similar tendency to SOD activities, but were not significantly different among the groups. Liver TBARS of WE-3 WE and AE-6 groups were slightly lower than those of other groups. Microscopic observation of liver tissue revealed that pine needle extracts increased cellular swelling, which was markedly increased in WE-6 group compared with control group.

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A cost-effective and simple culture method for primary hepatocytes

  • Adaya, Sezin;Hasircib, Nesrin;Gurhana, Ismet Deliloglu
    • Animal cells and systems
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    • v.15 no.1
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    • pp.19-27
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    • 2011
  • Hepatocytes, the major epithelial cells of the liver, maintain their morphology in culture dishes coated with extracellular matrix (ECM) components such as collagen and fibronectin or biodegradable polymers (e.g. chitosan, gelatin). In these coated dishes, survival of cells and maintaining of liver-specific functions may increase. The aim of this study was to determine a suitable, cost-effective and simple system for hepatocyte isolation and culture which may be useful for various applications such as in vitro toxicology studies, hepatocyte transplantation and bioartificial liver (BAL) systems. In order to obtain primary cultures, hepatocytes were isolated from liver by an enzymatic method and cultured on plates coated with collagen, chitosan or gelatin. Collagen, gelatin-sandwich and gelatin-cell mixture methods were also evaluated. Morphology and attachment of the cells were observed by inverted microscope and scanning electron microscope (SEM). An MTT assay was used to determine cell viability and mitochondrial activity.

Detailed Analysis on the Toxic Effect of Bisphenol A to the Liver and Testis in the Rat

  • Choi Ju-Yun;Yoo Min
    • Biomedical Science Letters
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    • v.11 no.3
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    • pp.333-336
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    • 2005
  • Environmental endocrine disruptors are very toxic to the animals including humans. They are hormone-like acting chemicals which can be found in our normal daily life. We have examined the toxic effect of bisphenol A and if lactic acid bacteria could suppress this toxic effect. Thirty rats were divided into three groups (control, bisphenol A treated, bisphenol A and lactic acid bacteria treated). Treatments were carried out at an interval of 12 hours for each group. Control group showed normal and clear morphology of tissues. Cells were fine in their shape and color, and density was high enough for the normal function. However, bisphenol A treated group was abnormally destructed in cell morphology. In the testis, sperms were totally destructed. When treated with lactic acid bacteria together, the toxic effect of bisphenol A was clearly decreased. This study indicated that bisphenol A was toxic in any concentration especially for the liver and testis, however, lactic acid bacteria could suppress the toxic effects of bisphenol A.

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Improvement Effect of Capsaicin Against Orotic Acid-Induced Fatty Liver in Rats (Orotic Acid-유발 지방간에 미치는 Capsaicin의 개선효과)

  • 전방실;차재영;유기수;조영수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.5
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    • pp.815-820
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    • 2004
  • The effect of capsaicin (0.02%) on the concentrations of liver and serum lipids, GOT and GPT activities, and hepatocyte morphology in male Sprague Dawley rats fed diets with or without erotic acid (1.0%) for 28 days was studied. Liver triglyceride concentration was increased by approximately 4.7-fold after erotic acid-feeding, wherease simultaneous feeding of capsaicin and erotic acid reduced its concentration by 38%. Orotic acidfeeding elevated liver cholesterol concentration, but an addition of capsaicin to the erotic acid diet resulted in the significant decrease in liver cholesterol. Capsaicin alone did not affect the liver triglyceride and cholesterol concentrations. Reduction of the serum concentrations of triglyceride and cholesterol by capsaicin was also observed in rats fed diets with erotic acid. The serum GOT and GPT activities were not significantly different among the experimental groups. Liver tissue morphology showed that the hepatocytes of erotic acid-feeding rats were a typical fatty liver with numerous fat droplets, whereas simultaneous feeding of capsaicin with erotic acid reduced the size and numbers of fat droplets. The present study demonstrated that the diet containing 0.02% capsaicin can prevent the erotic acid-induced fatty liver.

Pathologic differential diagnosis of metastatic carcinoma in the liver

  • Park, Jeong Hwan;Kim, Jung Ho
    • Clinical and Molecular Hepatology
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    • v.25 no.1
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    • pp.12-20
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    • 2019
  • The liver is one of the most common sites to which malignancies preferentially metastasize. Although a substantial number of liver malignancies are primary tumors, including hepatocellular carcinoma and intrahepatic cholangiocarcinoma, the metastasis of carcinomas to the liver is relatively common and frequently encountered in clinical settings. Representative carcinomas that frequently metastasize to the liver include colorectal carcinoma, breast carcinoma, neuroendocrine tumors, lung carcinoma, and gastric carcinoma. The diagnostic confirmation of suspected metastatic lesions in the liver is generally achieved through a histopathologic examination of biopsy tissues. Although morphology is the most important feature for a pathologic differential diagnosis of metastatic carcinomas, immunohistochemical studies facilitate the differentiation of metastatic carcinoma origins and subtypes. Useful immunohistochemical markers for the differential diagnosis of metastatic carcinomas in the liver include cytokeratins (CK7, CK19, and CK20), neuroendocrine markers (CD56, synaptophysin, and chromogranin A), and tissue-specific markers (CDX2, SATB2, TTF-1, GCDFP-15, mammaglobin, etc.). Here, we provide a brief review about the pathologic differential diagnosis of major metastatic carcinomas in the liver.

Fully Automatic Liver Segmentation Based on the Morphological Property of a CT Image (CT 영상의 모포러지컬 특성에 기반한 완전 자동 간 분할)

  • 서경식;박종안;박승진
    • Progress in Medical Physics
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    • v.15 no.2
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    • pp.70-76
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    • 2004
  • The most important work for early detection of liver cancer and decision of its characteristic and location is good segmentation of a liver region from other abdominal organs. This paper proposes a fully automatic liver segmentation algorithm based on the abdominal morphology characteristic as an easy and efficient method. Multi-modal threshold as pre-processing is peformed and a spine is segmented for finding morphological coordinates of an abdomen. Then the liver region is extracted using C-class maximum a posteriori (MAP) decision and morphological filtering. In order to estimate results of the automatic segmented liver region, area error rate (AER) and correlation coefficients of rotational binary region projection matching (RBRPM) are utilized. Experimental results showed automatic liver segmentation obtained by the proposed algorithm provided strong similarity to manual liver segmentation.

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Segmentation and 3-Dimensional Reconstruction of Liver using MeVisLab (MeVisLab을 이용한 간 영역 분할 및 3차원 재구성)

  • Shin, Min-Jun;Kim, Do-Yeon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.8
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    • pp.1765-1772
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    • 2012
  • Success rate of transplantation of body organs improved due to development of medical equipment and diagnostic technology. In particular, a liver transplant due to liver dysfunction has increased. With the development of image processing and analysis to obtain the volume for liver transplantation have increased the accuracy and efficiency. In this thesis, we try to reconstruct the regions of the liver within three dimensional images using the mevislab tool, which is effective in quick comparison and analysis of various algorithms, and in expedient development of prototypes. Liver is divided by applying threshold values and region growing method to the original image, and by removing noise and unnecessary entities through morphology and region filling, and setting of areas of interest. It is deemed that high temporal efficiency, and presentation of diverse range of comparison and analysis module application methods through usage of MeVisLab would make contribution towards expanding of baseline of medical image processing researches.