• Title, Summary, Keyword: lymphocystis

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Studies on Lymphocystis Diseases in Sebastes schlegeli (조피볼낙(Sebastes schlegeli)에 유행(流行)한 Lymphocystis병에 대(對)하여)

  • Chun, Seh-Kyu
    • Journal of fish pathology
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    • v.1 no.2
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    • pp.73-76
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    • 1988
  • There prevailed lymphocystis disease with 1 to 2cm in size in the skin and pins of cultured Sebastes schlegeli in fish farms in Tongyoung-gun, Sanyang-myeon, Kon-ri from summer, 1987 to fall, 1988. Though there were some difference between each fish farm, this disease prevailed widely from immature fish to mature fish, less than 10cm to 30cm in body length with about 8 through 80% of infection rate. Sebastes schlegeli with the lymphocystis disease showed combined nipple-shaped mass by doubly, triply propagated lymphocystis. But the author considered that lymphocystis disease was not a direct cause of death of Sebastes schlegeli, the fish showed marked inflammation by seconary infection of pathogenic organisms.

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A Genetic Marker Associated with Resistance to Lymphocystis Disease in the Olive Flounder, Paralichthys olivaceus (넙치 Lymphocystis 바이러스 질병 내성 유전자 Marker)

  • Kang, Jung-Ha;Nam, Bo-Hae;Han, Hyon-Sob;Lee, Sang-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.40 no.3
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    • pp.128-132
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    • 2007
  • We identified a microsatellite marker, Poli121TUF, which appears to be significantly linked (P<0.001) with a lymphocystis disease virus (LCDV)-resistance gene in the olive flounder, Paralichthys olivaceus. The olive flounder is an economically important food fish, that is widely cultured in Korea, Japan, and China. Lymphocystis disease has spread in these countries and has seriously reduced the economic value of the fish. LCDV causes lymphocystis cells (LC) to form on the body surface, fins, gills, mouth, and intestine. Fish with LC lose commercial value due to their deformed appearance. The identified micro satellite marker can be used as a candidate locus for marker-assisted selection (MAS) in order to enhance the efficiency of selection for LCDV resistance in the olive flounder.

Pathological findings of lymphocystis in cultured flounders (양식넙치에 발생한 lymphocystis의 병리학적 소견)

  • Kim, Soon-bok
    • Korean Journal of Veterinary Research
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    • v.40 no.2
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    • pp.333-337
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    • 2000
  • This experiment was done to observe the pathological findings of lymphocystis naturally occurred in the cultured flounders in the southern sea of Korea. Anatomical sign of lymphocystis was characterized by the presence of wart-like nodules on the fins, body surface and mouth. Dense clustering of hypertrophic cells originated from fibroblasts was observed in the lesions. Infected hypertrophic cells had a thick hyaline capsule, large vesiculated nucleus with irregular rims and large nucleolus, and large ribbon-shaped basophilic inclusions at the peripheral zone of the cytoplasm. Hexagonal virus particles with the two layers of capsid were scattered throughout the cytoplasm and were absent from the inclusions.

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Differentiation of Lymphocystis Disease Virus Genotype by Multiplex PCR

  • Kitamura Shin Ichi;Jung Sung-Ju;Oh Myung-Joo
    • Journal of Microbiology
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    • v.44 no.2
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    • pp.248-253
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    • 2006
  • Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease. The viruses have been divided into three genotypes (genotype I for LCDV-1, II for Japanese flounder isolates, and III for rockfish isolates) on the basis of major capsid protein (MCP) gene sequences. In this study, we developed a multiplex PCR primer set in order to distinguish these genotypes. We also analyzed the MCP gene of a new LCDV isolate from the sea bass (SB98Yosu). Comparison of sequence identities between SB98Yosu and eight Japanese flounder isolates, revealed identity of more than 90.1 % at nucleotide level and 96.5% at deduced amino acid level, respectively. Phylogenetic analyses based on the MCP gene showed that SB98Yosu belongs to genotype II, along with Japanese flounder isolates. Multiplex PCR based on the MCP gene allowed us to identify these genotypes in a simple and rapid manner, even in a sample that contained two genotypes, in this case genotypes II and III.

Identification of antigenic proteins of lymphocystis disease virus (LCDV) by MALDI-TOF mass spectrometry

  • Chung, Chang-Kyun;Kim, Byung-Gwan;Jung, Myung-Hwa;Jung, Sung-Ju
    • Journal of fish pathology
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    • v.28 no.3
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    • pp.133-143
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    • 2015
  • The antigenic proteins of Lymphocystis disease virus (LCDV) from tumors of olive flounder, Paralichthys olivaceus, are described following characterization by mass spectrometry. In SDS-PAGE, predominant protein bands were observed at 114, 88, 70, 54, 52, 47, 42 and 24 kDa. Western blot analysis showed that antisera reacted strongly at molecular weights of 114, 67 and 54 kDa, and reacted weakly at molecular weights of 74, 70, 36, 24 and 22 kDa. In the identification of LCDV antigenic proteins by matrix-assisted laser desorption ionization (MALDI) TOF mass spectrometry, 10 of 14 excised bands consisted mostly of proteins with amino acid sequences that matched LCDV-C (lymphocystis disease virus isolate China) ORFs. Strong antigens with molecular weights of 114, 67 and 54 kDa were identified as LDVICp236 (chromosome segregation ATPase), LDVICp033 (membrane bound metallopeptidase) and LDVICp157 (hypothetical protein), respectively. Minor antigens with molecular weights of 70, 36, 24 and 22 kDa proteins were identified as LDVICp160 (acetyl-coA hydrolase), LDVICp213 (hypothetical protein), LDVICp039 (hypothetical protein) and LDVICp213 (hypothetical protein). However, the major capsid protein (LDVICp043) did not react with the polyclonal antibody.

The serodiagnosis of a lymphocystis disease virus infection using an antibody raised against a recombinant major capsid protein

  • Seo, Ja-Young;Kang, Bong-Jo;Oh, Hyoung-Jong;Lee, Jae-Il;Kim, Tae-Jung
    • Journal of fish pathology
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    • v.21 no.3
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    • pp.175-180
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    • 2008
  • Lymphocystis is a viral disease of fish primarily in marine and brackishwaters. Here we report the cloning, expression, and the serological applications of the lymphocystis disease virus (LCDV) major capsid protein (MCP). The MCP gene was amplified by PCR from the genomic DNA of LCDV isolated from Schlegel's black rockfish, Sebastes schlegeli, and expressed in E. coli. Mouse antisera raised against the purified recombinant MCP (rMCP) reacted with the viral MCP in an immunofluorescence assay, indicating that this rMCP would be useful for serological studies of field samples.

양식 넙치, Paralichthys olivaceus에서 분리된 림포시스티스 바이러스의 genomic DNA 분석과 PCR을 이용한 바이러스 검출

  • 김수미;박수일;손상규;박명애
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • pp.436-437
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    • 2000
  • Iridoviridae과에 속하는 lymphocystis disease virus(LDV)는 비교적 큰 20면체 DNA 바이러스이며(Murphy et al., 1995), 림포시스티스병(lymphocystis disease, LD)을 유발하는 원인체로서 140여종 이상의 해수 및 담수 어종에서 보고된 바 있다(Wolf et al., 1966). 우리 나라의 경우, 주요 양식 어종인 넙치에 주로 감염되어 2차 감염과 빈혈 및 합병증 등에 의해 폐사를 유발할 뿐만 아니라 상품성을 저하시킴으로서 경제적 손실을 야기한다. (중략)

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Comparison of lymphocystis disease virus proteins between marine and freshwater fish

  • Hossain, Mosharrof;Jung, Sung-Ju;Kim, Wi-Sik;Kim, Seok-Ryel;Oh, Myung-Joo
    • Journal of fish pathology
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    • v.22 no.2
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    • pp.173-177
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    • 2009
  • Lymphocystis disease virus (LCDV) was detected from olive flounder Paralichthys olivaceus, painted glass fish Chanda baculis, gourami Trichogaster leeri and rockfish Sebastes schlegeli, and proteins of the viruses were compared. The major capsid protein (MCP) gene-specific primer sets successfully amplified approximately 1300 bp nucleotides from the olive flounder and 600 bp nucleotides from painted glass fish, gourami and rockfish isolates, respectively. In western blotting analysis using anti-LCDV mouse polyclonal serum, major antigenic proteins had 21, 26, 45, 50, 80, 110 and 120 kDa in olive flounder, 26, 47 and 80 kDa in painted glass fish, 26, 46, 80 and 92 kDa in gourami, 26, 44, 49, 80 and 105 in rockfish, respectively. All the marine and freshwater isolates showed only common antigens of approximately 26 kDa and 80 kDa. These results suggest that antigenic protein profiles of LCDVs may vary depending upon fish species.

Propagation of lymphocystis disease virus (LCDV) in the FIN cell line originated from olive flounder Paralichthys olivaceus fin

  • Hossain, Mosharrof;Kim, Wi-Sik;Jung, Sung-Ju;Oh, Myung-Joo
    • Journal of fish pathology
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    • v.24 no.2
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    • pp.47-51
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    • 2011
  • The present study demonstrated lymphocystis disease virus (LCDV) propagation through cytopathic effects (CPE) formation and LCDV detection in olive flounder fin (FFN) cells by polymerase chain reaction (PCR) and fluorescent antibody technique (FAT) methods. Tissue filtrates from the cluster cells produced CPE in FFN cells, which initially cells became enlarged and gradually underwent fusion en masse. Infectivity of culture grown LCDV using the FFN cells reached $10^{2.3}$ $TCID_{50}$/ml at 4 days post infection and the highest titer was measured $10^{6.5}$ $TCID_{50}$/ml at 12 days. The viral DNA was detected in the cell culture supernatants showing CPE and the CPE cells by PCR. Antigen specific strong fluorescence reacting with monoclonal antibody against the virus revealed the presence of viral antigen in the cytoplasm of infected FFN cells. These results suggest that the FFN cell line originated from the olive flounder has a susceptibility of the LCDV.

양식돌돔 폐사를 유발하는 이리도바이러스의 특성

  • 도정완;박미선;손상규;최동림;방종득;이주석
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • pp.434-435
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    • 2000
  • 참돔 이리도바이러스 질병(red seabream iridovirus disease ; RSIVD)은 1990년 일본 시코쿠지역의 참돔 양식장에서 처음 발병된 후, 매년 발병지역이 확산되고 발병 어종이 다양해지고 있다. 참돔에서 분리된 RSIV는 icosahedral cytoplasmic deoxyribovirus로서 크기가 200∼240nm이며 형태학적 특징에 의해 iridoviridae로. 분류하고 있지만, 어류를 숙주로 하는 iridovirus과 중에서 lymphocystis virus속인 flounder virus(LCDV-1) 및 lymphocystis disease virus(LCDV-2)와 goldfish virus 1-lke virus속인 goldfish virus 1(GFV-1) 및 goldfish virus 2(GFV-2)와는 전혀 다른 바이러스로 알려져 있다. (중략)

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