• Title, Summary, Keyword: lymphocytes

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LPS Stimulated B Lymphocytes Inhibit the Differentiation of Th1 Lymphocytes (LPS에 의해 자극된 B 림프구에 의한 Th1 림프구 분화 억제)

  • Kim, Ha-Jeong
    • Journal of Life Science
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    • v.25 no.12
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    • pp.1425-1431
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    • 2015
  • The lymphocyte component of the immune system is divided into B lymphocytes and T lymphocytes. B lymphocytes produce antibodies (humoral immunity) via maturation into plasma cells, and T lymphocytes kill other cells or organisms (cellular immunity). A traditional immunological paradigm is that B lymphocyte and T lymphocyte interactions are a one-way phenomenon, with T lymphocytes helping to induce the terminal differentiation of B lymphocytes into immunoglobulin class-switched plasma cells. A deficiency of T lymphocytes was reported to result in defective B lymphocyte function. However, evidence for a reciprocal interaction between B and T lymphocytes is emerging, with B lymphocytes influencing the differentiation and effector function of T lymphocytes. For example, B lymphocytes have been shown to induce direct tolerance of antigen-specific CD8+ T lymphocytes and induce T lymphocytes anergy via transforming growth factor-beta (TGF-β) production. The present study showed that LPS-stimulated B lymphocytes inhibited the differentiation of Th1 lymphocytes by inhibiting the production of interleukin-12 (IL-12) from dendritic cells. An interaction between the B lymphocytes and dendritic cells was not needed for this inhibition, and the B lymphocytes did not alter dendritic cell maturation. B lymphocyte-derived soluble factor (BDSF) suppressed the LPS-induced IL-12p35 transcription in the dendritic cells. Overall, these results point to a novel B lymphocyte- mediated immune suppressive mechanism. The findings cast doubt on the traditional paradigm of immunological interactions involving B lymphocyte and T lymphocyte interactions.

Stimulatory Effect of Korean Red-Ginseng Extract on the Proliferation and . Cellular Activity of Lymphocytes (홍삼 추출물의 임파구 증식 및 활성 촉진효과)

  • 이혜연;이한수
    • Journal of Ginseng Research
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    • v.22 no.1
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    • pp.60-65
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    • 1998
  • The effect of Korean red-ginseng extract on the proliferation and cellular activity of mouse B and T lymphocytes was examined in vitro. Both water and ethanol extract from red-ginseng increased the growth of normal B and T lymphocytes 1.5∼2.5-folds. Saponin and polysaccharide fractions from ginseng extract also stimulated the proliferation of normal lymphocytes much higher than several well-known immunostimulators. B and T lymphoma cell lines responded to the ginseng extract and fractions by growth, too, while non-lymphoid cell lines did not. Immunoglobulin production of unprimed B-lymphocytes was little affected by the ginseng extract and fractions, though the ethanol extract slightly enhanced Ini, production of B-lymphocytes. When cytolytic activity of T lymphocytes against tumor tells was induced in vitro, both of the saponin and polysaccharide fractions and the ginseng ethanol extract increased the cellular activity of cytotoxic T lymphocytes 4-5-folds, while the ginseng water extract did not. Especially, the saponin fraction exhibited 10-times higher stimulatory effect on the cytolytlc activity of cytotoxic T cells than the ethanol extract and the pclysaccharide fraction did. These results suggest that Korean red-ginseng contain potent immunomodulating components to stimulate the proliferation of B and T lymphocytes and the cellular activity of cytotoxic T lymphocytes.

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Experimental Study on the Effect of Houttuyniae herba Aqua-acupuncture on Immune Responses in Irradiated Rats (어성초약침(魚腥草藥鍼)이 방사선(放射線) 피폭(被曝)에 의한 면역기능(免疫機能) 저하(低下)에 미치는 영향(影響))

  • Jung, Sung-Ki
    • The Journal of Korean Medicine
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    • v.18 no.2
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    • pp.97-107
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    • 1997
  • The study was performed to investigate the effect of Houttuyniae herba Aqua- acupuncture on immune responses in irradiated rats. The results are as follows; 1. In the assay of peripheral lymphocytes, Houttuyniae herba aqua-acupuncture showed protective effect on peripheral lymphocytes from irradiation. 2. In the assay of $CD_4\;&\;CD_8$ T lymphocytes, Houttuyniae herba aqua-acupuncture showed pretective effect on $CD_4\;and\;CD_8$ T lymphocytes from irradiation with no stastistical significance. 3. In the assay of spleen lymphocytes, Houttuyniae herba aqua-acupuncture showed pretective effect on lymphocytes from irradiation with stastistical significance. 4. In the assay of spleen $CD_4$ T lymphocytes, Houttuyniae herba aqua-acupuncture showed pretective effect on $CD_4$ T lymphocytes from irradiatian with stastistical significance. 5. In the study of $CD_8$ T lymphocytes, Houttuyniae herba aqua-acupuncture showed pretective effect on $CD_4$ T lymphocytes from irradiation with no stastistical significance These results show that Houttuyniae herba aqua-acupuncture is an effective therapy upon immune deficiency induced by irradiation.

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IMMUNOHISTOCHEMICAL STUDY ON LYMPHOCYTE DISTRIBUTION IN ENDODONTICALLY TREATED AND UNTREATED PERIAPICAL LESIONS (근관치료전과 후의 치근단 병소에서 임파구의 분포에 관한 면역조직화학적 연구)

  • Oh, Tae-Seok;Lim, Sung-Sam
    • Restorative Dentistry and Endodontics
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    • v.11 no.1
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    • pp.63-75
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    • 1985
  • This study was designed to identify lymphocytes and to compare the lymphocyte distribution in endoodontically treated periapical lesions with that in endodontically untreated periapical lesions by way of immunohistochemical staining. Twenty-one human dental periapical lesions were obtained, frozened, serially sectioned to $4-5{\mu}$, and stained using the three-stage indirect immunoperoxidase technique and monoclonal antibodies for detecting the presence of B,T lymphocyte and T suppressor cell. Following results were obtained; 1. All of the examined periapical lesions had positive staining for B,T lymphocyte and T suppressor cell. 2. The concentration of T lymphocytes in 18 lesions diagnosed as periapical cyst and granuloma in both groups was greater than that of B lymphocytes and 2 periapical lesions identified as abscess in treated lesions had more positive B lymphocytes than positive T lymphocytes. 3. The average numbers of T,B lymphocytes and T suppressor cells in Endodontically treated lesions were lower than those of untreated lesions, but no statistically significant difference was noted. 4. When the distribution ratios of T lymphocytes to B lymphocytes and T suppressor cells to T lymphocytes were compared in Endodontically treated lesions by the histological aspects of the lesions and at the intervals of the duration after Endodontic treatment, a statistically significant change was not found. 5. The mean values of T lymphocytes, B lymphocytes and T suppressor cells in Endodontically treated lesions were markedly decreased in the specimens obtained at 3 month after Endodontic treatment, but no statistically significant difference was found.

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A QUANTITATIVE ANALYSIS OF THE IMMUNOCOMPETENT CELLS IN PERIAPICAL LESIONS OF THE HUMAN TEETH (치근단 병소에서 면역적격세포의 분포에 관한 연구)

  • Yoon, Tai-Cheol;Kim, Jin;Park, Dong-Soo
    • Restorative Dentistry and Endodontics
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    • v.17 no.1
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    • pp.55-68
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    • 1992
  • Periapical lesions are developed as a result of inflammatory response to irritants from root canal system. Clinicians remove these irritants from root canal system and seal the root canal space to induce healing of the periapical lesions. Immunopathologic responses may play an important role in development and progression of periapical lesions and periapical lesions contain immunocompetent cells. The purposes of the present study were to analys and to compare the distribution of the immunocompetent cells in the human periapical lesions according to the stage of endodontic treatment using indirect immunoperoxdase technique. Obtained 94 human periapical lesions were devided into four groups: Group 1 : no endodontic treatment(28 samples) Group 2 : root canal enlarged and irrigated(28 samples) Group 3 : root canal filled(29 samples) Group 4: unknown(9 samples) Monoclonal antibodies to examine target cells were UCHL-1 for T lymphocytes(1 : 200, Dakopatt, Denmark), L26 for B lymphocytes(1 : 200, Dakopatt, Denmark), OPD4 for helper T lymphocytes(l : 200, Dakopatt, Denmark) and alpha-1-antichymotrypsin for macrophages(l : 2000, Dakopatt, Denmark). The following results were obtained : 1. All the periapical lesions studied were infiltrated by T lymphocytes, plasma cells, B lymphocytes, and macrophages. T lymphocytes were more infiltrated than B lymphocytes, and B lymphocytes and macrophages were less infiltrated than T lymphocytes and plasma cells(P<0.05 : Oneway ANOVA test). 2. In untreated group and canal irrigated and enlarged group of all the periapical lesions, helper T lymphocytes were predominently infiltrated(P>0.05 : Oneway ANOVA test). 3. In canal filled groups of all lesions except periapical cyst, plasma cells were predominently infiltrated. But, in canal filled group of periapical cyst, helper T lymphocytes were the predominent cells(P>0.05 : Oneway ANOVA test). The above results shows that the immunologic responses play important role in pathogenesis of periapical lesions and the immunologic response involved undergoes certain changes after endodontic therapy.

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The effect of Boa-tang to the lymphocytes (보아탕(補兒湯)이 림프구의 증식능(增殖能)에 미치는 영향(影響))

  • Im, Jae-Gook;Hong, Moo-Chang;Ha, Yoon-Moon
    • Journal of Oriental Physiology
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    • v.14 no.2
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    • pp.83-97
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    • 1999
  • By inserting Boa-tang into culture median of lymphocytes from spleen of rat and lymphocytes in human peripheral blood The results were as follow. 1. In case of spleen lymphocytes of rat, cultures of lymphocytes did not significantly increased which were inserted Boa-tang $100{\mu}g/ml$, and which were inserted $10{\mu}g/ml$ at 3rd day 2. In case of spleen lymphocytes of rat, cultures of lymphocytes were significantly increased which were inserted Boa-tang $10{\mu}g/ml$ with Con A $2.5{\mu}g/ml$ at 2nd, 3rd day, and Boa-tang $1{\mu}g/ml$ with ConA $2.5{\mu}g/ml$ at 3rd day than inserted ConA $2.5{\mu}g/ml$ 3. In case of lymphocytes in human peripheral blood, cultures of lymphocytes were significantly increased which were inserted Boa-tang $100{\mu}g/ml$ and $10{\mu}g/ml$ at 2nd day to 5st day, and which were inserted Boa-tang $1000{\mu}g/ml$ at 2nd day to 5th day, and which were inserted $1{\mu}g/ml$ at 2nd day By looking at the following results, in case of rat cultures of lymphocytes were significantly increased which were inserted the lower density, but in case of human cultures of lymphocytes were significantly increased which were inserted the higher density.

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Effects of low-level exposure to manganese and lead on immune function (저 농도의 망간과 납 노출이 면역기능에 미치는 영향)

  • Kim, Ki-Woong;Park, SangHwoi;Won, Yong Lim;Lee, Sung Kwang
    • Analytical Science and Technology
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    • v.27 no.5
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    • pp.248-253
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    • 2014
  • This study aimed to evaluate the effects of exposure to manganese (Mn) and lead (Pb) on immune system. The subjects were 42 male workers, among whom 13 office workers (Group I) had never been occupationally exposed to heavy metals, 21 were worked in manufacturing factories (Group II) and 8 were welders (Group III). The mean blood Mn and Pb level by groups were significantly different. The numbers of CD19+ and total lymphocytes in Group I were significantly higher than those in other groups, but no significant differences were found in other T lymphocytes subpopulation. Mn and Pb concentrations showed negative correlation with T lymphocytes subpopulation, but Mn concentrations were statistical significances with T lymphocytes subpoplation except CD4+CD45RO+ and natural killer cell. Pb concentration was only statistical significance with total lymphocytes. Our results suggest that occupationally exposed to Mn and Pb can affect the cellular immune response.

The Proliferative and Apoptotic Properties of Nile Tilapia (Oreochromis niloticus) sIg+ Lymphocytes by Cortisol Treatment

  • Park, Kwan-Ha;Choi, Sang-Hoon
    • Journal of Animal Science and Technology
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    • v.53 no.6
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    • pp.563-569
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    • 2011
  • The effects of cortisol on proliferation and apoptosis of tilapia surface immunoglobulin positive ($sIg^+$) lymphocytes isolated from different tissues were investigated. $sIg^+$ lymphocytes from the tilapia head kidney (HK) and spleen showed a higher proliferation and lower intracellular calcium ($Ca^{2+}{_i}$) level to Ig-crosslinking compared with peripheral blood $sIg^+$ lymphocytes. Peripheral blood $sIg^+$ lymphocytes stimulated with lipopolysaccharide (LPS) showed high levels of apoptosis in the presence of cortisol. HK and to a lesser extent spleen $sIg^+$ lymphocytes, although less sensitive than their equivalent in peripheral blood, showed cortisol-induced apoptosis irrespective of LPS stimulation of control levels. Compared to plasma values measured during stress conditions, proliferation regardless of LPS stimulation was apparently suppressed by cortisol that is effective in inducing a significant increase in apoptosis in all three different cell populations of $sIg^+$ cells, suggesting the immunoregulatory effect of cortisol in both LPS stimulated and non-stimulated conditions. Different sensitivity of $sIg^+$ cells to the cortisol, in regard to developmental stage and activity, could be related in inhibiting excessive and continuing depletion of $sIg^+$ lymphocytes.

The Effect of Cortisol on Proliferative Properties of Flounder (Paralychthys Olivaceus) B Lymphocytes

  • Choi, Sang-Hoon;Oh, Chan-Ho
    • Animal cells and systems
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    • v.7 no.1
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    • pp.57-62
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    • 2003
  • Flounder B lymphocytes isolated from different tissues were studied in terms of cell proliferation, apoptosis and the effects of cortisol on these processes. B lymphocytes, isolated from the flounder head kidney and spleen, were characterized by higher proliferation and lower intracellular calcium ($Ca^2$) response to lgcrosslinking compared with peripheral blood B lymphocytes. Cortisol induced high levels of apoptosis (150% of control levels) in peripheral blood B lymphocytes, in combination with a stimulatory LPS signal. Head kidney and to a lesser extent spleen B lymphocytes, although less sensitive than their equivalent in peripheral blood, underwent cortisol-induced apoptosis irrespective of extra stimulation up to 142% of control levels. Also proliferation with and without LPS stimulation was suppressed by cortisol (compared to plasma values measured during stress conditions) that is effective in inducing a significant increase in apoptosis in all three populations of B-cells, suggesting that cortisol may be important for immunoregulation in both stressed and non-stressed conditions. This implies possible severe impact of stress on lymphocyte development and activity, Different sensitivity of B-cells to the corticosteroid, with respect to developmental stage and activity, may prevent excessive and long lasting depletion of B-lymphocytes.

An Increased Proportion of Apoptosis in CD4+ T Lymphocytes Isolated from the Peripheral Blood in Patients with Stable Chronic Obstructive Pulmonary Disease

  • Ju, Jinyung
    • Tuberculosis and Respiratory Diseases
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    • v.81 no.2
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    • pp.132-137
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    • 2018
  • Background: The pathophysiology of chronic obstructive pulmonary disease (COPD) includes inflammation, oxidative stress, an imbalance of proteases and antiproteases and apoptosis which has been focused on lately. Abnormal apoptotic events have been demonstrated in both epithelial and endothelial cells, as well as in inflammatory cells including neutrophils and lymphocytes in the lungs of COPD patients. An increased propensity of activated T lymphocytes to undergo apoptosis has been observed in the peripheral blood of COPD patients. Therefore, the apoptosis of T lymphocytes without activating them was investigated in this study. Methods: Twelve control subjects, 21 stable COPD patients and 15 exacerbated COPD patients were recruited in the study. The T lymphocytes were isolated from the peripheral blood using magnetically activated cell sorting. Apoptosis of the T lymphocytes was assessed with flow cytometry using Annexin V and 7-aminoactinomycin D. Apoptosis of T lymphocytes at 24 hours after the cell culture was measured so that the T lymphocyte apoptosis among the control and the COPD patients could be compared. Results: Stable COPD patients had increased rates of $CD4^+$ T lymphocyte apoptosis at 24 hours after the cell culture, more than the $CD4^+$ T lymphocyte apoptosis which appeared in the control group, while the COPD patients with acute exacerbation had an amplified response of $CD4^+$ T lymphocyte apoptosis as well as of $CD8^+$ T lymphocyte apoptosis at 24 hours after the cell culture. Conclusion: Stable COPD patients have more apoptosis of $CD4^+$ T lymphocytes, which can be associated with the pathophysiology of COPD in stable conditions.