• Title, Summary, Keyword: macrophage

Search Result 2,217, Processing Time 0.044 seconds

Effects of Tokhwalkisaengtang on LDL Oxidation in Macrophage Cell (대식세포(大食細胞) oxLDL 생성(生成)에 미치는 독활기생탕(獨活寄生湯)의 영향(影響))

  • Hwang Gwi-Seo;Song Ji-Yeon
    • Journal of Society of Preventive Korean Medicine
    • /
    • v.4 no.2
    • /
    • pp.205-213
    • /
    • 2000
  • The oxidative modification of low density lipoprotein(LDL) has been implicated in the development of atherosclerosis . Oxidized LDL(oxLDL) are found in macrophage foam cell , and it can induce an macrophage proliferation in atherosclerotic plaque. In this study, we investigated the hypothesis that Tokhwalkisaengtang(TK) may reduce atherosclerosis by lowering the oxidizability of LDL, To achieve this goal, we examined the effect of TK on LDL oxidation, nitric oxide production in murine macrophage cell line , and the effect of TK on cupuric sulfate-induced cytotoxicity. LDH release, and macrophage activity TK inhibited the generation of oxLDL from native LDL in macrophage cell culture, and decreased the release of LDH from cupric sulfate-stimulated macrophage. In other experiments, TK activated macrophase cell, and increased the survival rate, and enhanced nitric oxide production in macrophage.

  • PDF

Effects of Quercus dentata and Quercus acutissima Extracts on the Activations of Macrophage RAW264.7 Primed with $IFN-{\gamma}$ (곡피(?皮)와 상목피(橡木皮) 추출물이 대식세포 RAW264.7 활성화에 미치는 영향)

  • Jo, Jin-Hee;Seong, Nak-Sull;Lee, Young-Jong
    • The Korea Journal of Herbology
    • /
    • v.21 no.1
    • /
    • pp.89-100
    • /
    • 2006
  • Objectives : The effects of methanol extracts from the cortex of Quercus dentata Thunb. and Quercus acutissima Carruth, on the activation of macrophage were examined. Methods : Methanol extracts of Quercus dentata (QD) and Quercus acutissima (QA) were applied to cell line RAW264,7 (macrophage), and their effects were examined. Results : 1. Extracts from QD and QA had no specific influence on the cell growth. 2. Extracts from QD and QA did not activate macrophage independently, but the addition of $IFN-{\gamma}$ facilitated the generation of macrophage's nitric oxide(NO). 3. QD and QA extracts increased the manifestation of iNOS gene, when macrophage was activated by $IFN-{\gamma}$. 4. QD and QA extracts increased the manifestation of $TNF-{\alpha}$ in macrophage, which took 2 hours. 5. QD and QA extracts increased the generation of $PGE_2$ in macrophage. Conclusion : QD and QA activate macrophage in the presence of $IFN-{\gamma}$. After activation is primarily facilated by $IFN-{\gamma}$, it works on macrophage secondarily for the manifestation of iNOS gene and for the generation of $TNF-{\alpha}$.

  • PDF

Fermented Artemisiae Argyi Folium and Epimedii Herba Mixture Effect on Macrophage' Activity (애엽과 음양곽 혼합 발효물이 대식세포 활성에 미치는 영향)

  • Ryu, Hahn-Woo;Kim, Yoon-Sang;Lee, Eun-Mee
    • The Journal of Korean Obstetrics and Gynecology
    • /
    • v.22 no.2
    • /
    • pp.79-93
    • /
    • 2009
  • Purpose: This research aimed to study the effect of FAE(Ferment Artemisiae Argyi Folium and Epimedii Herba) on the mouse macrophage cell activity. Methods: Effect of FAE, which was fermented by Sacchromyces cerevisiae STV89, on cell viability, amount of $H_2O_2$ within cells, amount of NO was measured and compaperd by using mouse macrophage cells. Results: 1. Result of MTT assay conducted to observe the effect of FAE on the survival rate of mouse macrophage cells illustrated that, when FAE was proccessed for each concentration, there was no significant decrease of the survival rate. 2. FAE increased the amount of $H_2O_2$ within macrophage cells and increased inhibition of amount of $H_2O_2$ in macrophage induced by LPS. 3. FAE inhibited amount of NO in macrophage cells, and significantly inhibited increase of amount of NO in mcacrophage induced by LPS. Conclusion: FAE produced by Artemisiae Argyi Folium and Epimedii Herba did not induce the decrease of macrophage cell survival rate, increased amount of $H_2O_2$ within cells, and reduced amount of NO. FAE significantly increase by LPS, reduced the increase of amount of NO in macrophage induced by LPS. These results signify FAE has significant effect on immuno modulating activity of macrophage.

  • PDF

Metabolic influence on macrophage polarization and pathogenesis

  • Thapa, Bikash;Lee, Keunwook
    • BMB Reports
    • /
    • v.52 no.6
    • /
    • pp.360-372
    • /
    • 2019
  • Macrophages play an essential role not only in mediating the first line of defense but also in maintaining tissue homeostasis. In response to extrinsic factors derived from a given tissue, macrophages activate different functional programs to produce polarized macrophage populations responsible for inducing inflammation against microbes, removing cellular debris, and tissue repair. However, accumulating evidence has revealed that macrophage polarization is pivotal in the pathophysiology of metabolic syndromes and cancer, as well as in infectious and autoimmune diseases. Recent advances in transcriptomic and metabolomic studies have highlighted the link between metabolic rewiring of macrophages and their functional plasticity. These findings imply that metabolic adaption to their surrounding microenvironment instructs activation of macrophages with functionally distinct phenotypes, which in turn probably leads to the pathogenesis of a wide spectrum of diseases. In this review, we have introduced emerging concepts in immunometabolism with focus on the impact on functional activation of macrophages. Furthermore, we have discussed the implication of macrophage plasticity on the pathogenesis of metabolic syndromes and cancer, and how the disease microenvironment manipulates macrophage metabolism with regard to the pathophysiology.

Effects of Ginseng Saponin Fraction and Cyclophosphamide on the Tumoricidal Activity of Mouse Macrophage and the Antitumor Effect (생쥐의 대식세포 종양치사활성과 항암효과에 미치는 인삼 Saponin 분획물과 Cyclophosphamide의 영향)

  • Jeon, Hye-Gyeong;Kim, Se-Chang;Jeong, No-Pal
    • Journal of Ginseng Research
    • /
    • v.15 no.2
    • /
    • pp.99-105
    • /
    • 1991
  • This experiment was performed to investigate the effects of ginseng saponin fraction and cyclophosphamide (CY) on the tumor development, the antitumor effect and the tumoricidal activity of mouse macrophage. When mice were treated with saponin or CY following inoculation with Sarcoma 180, tumor development was inhibited and survival ratio increased, and a combination of both treatments further inhibited the tumor development. Tumoricidal activity of macrophage was effectively increased at 10-7% concentration of CY and it was further increased when macrophage was cotreated with saponin and CY. Tumoricidal activity of macrophage was greatest at the third day after inoculating tumor cell. Both saponin and CY increased the chemiluminescence of macrophage, but CY had no effect on releasing TNF, unlike saponin.

  • PDF

생쥐 대식세포의 종양세포 치사활성에 미치는 인삼분획물과 지방다당류의 영향

  • Choe, Sang-Un;Jeong, No-Pal;Kim, Se-Chang
    • Journal of Ginseng Research
    • /
    • v.14 no.3
    • /
    • pp.364-372
    • /
    • 1990
  • This experiment was performed to investigate the effects of ginseng saponin fractions (total saponin, triol saponin. diol saponin) and lipopolysacrharide (LPS) on the tllmoricidal activity of macrophage. The ginseng saponin fractions had little effect on the tumoricidal activity of macrophage (less than 10%). When the ginseng saponin fractions were treated with LPS, the effects of tumoricidal activation of macrophage increased a relatively high percent, and the total saponin and triol saponin (20-35%) were ulore effectual than diol saponin (15-25%). The effects of ginseng saponin and LPS on the tumoricidal activity of macrophage were mediated by the induction of macrophage-release factor(5) which has(have) the capacity of tumor cell killing. And the quantity of the (actors) was(were) increased by the contact of macrophage with tumor cell.

  • PDF

Proinflammatory Effects of Bacterial Lipopolysaccharide (LPS) in Rainbow Trout (Oncorhynchus mykiss) Macrophage Cells

  • Hong Suhee;Jeong Hyun Do
    • Fisheries and aquatic sciences
    • /
    • v.6 no.3
    • /
    • pp.130-134
    • /
    • 2003
  • Proinflammatory effects of bacterial lipopolysaccharide (LPS) have been assessed by analysing the induction of two inflammatory genes, $interleukin-1\beta$ $(IL-1\beta)$ and cyclooxygenase-2 (COX-2), in rainbow trout (Oncorhynchus mykiss) macrophage cells. Production of a metabolite of arachidonic acid by COX-2, prostaglandin $E_2\;(PGE_2)$, was also analysed in macrophage cells after LPS stimulation. Northern blot analysis revealed that LPS $(5{\mu}g/mL)$ significantly upregulated $IL-1\beta$ (54 times) and COX-2 (40.7 times) gene expression in macrophage cells after 4 h stimulation. According to RT-PCR (Reverse Transcription Polymerase Chain Reaction) analysis, $IL-1\beta$ gene induction in LPS stimulated macrophage cells was started within 1h and significantly increased thereafter until 4h. Meanwhile, COX-2 gene induction by LPS was delayed in comparison with $IL-1\beta$ gene induction as a faint band was observed after 4h stimulation in head kidney macrophage cells. LPS also significantly increased $PGE_2$ production in head kidney leucocytes, presumably via activating COX-2 expression that metabolites arachidonic acid to $PGE_2$. In conclusion, it was demonstrated that LPS could induce two main inflammatory and immune related genes, $IL-1\beta$ and COX-2, and increase $PGE_2$ production in trout head kidney macrophage cells, representing a strong inflammatory activity.

Macrophage Stimulating Activity of Exo-Biopolymer from Submerged Culture of Lentinus edodes with Rice Bran

  • Yu, Kwang-Won;Shin, Kwang-Soon;Choi, Yang-Mun;Suh, Hyung-Joo
    • Journal of Microbiology and Biotechnology
    • /
    • v.14 no.4
    • /
    • pp.658-664
    • /
    • 2004
  • To find a new utilization of rice bran, nine higher fungi were examined for the production of exo-biopolymer with macrophage stimulating activity from rice bran. Among the exo-biopolymers produced from submerged cultures, Lentinus edodes showed the highest activity, followed by Grifola frondosa, Schizophyllum commune, and Coriolus versicolor. L. edodes also had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve rice bran utilization and the yield of exo-biopolymer with macrophage stimulating activity, the treatment of Rapidase effectively increased the macrophage stimulating activity (about 30% increase), whereas the other enzymes (Econase, Viscozyme, Ultraflo, Celluclast, and Thermylase) treatments did not increase the macrophage stimulating activity. Exo-biopolymer with macrophage stimulating activity from L. edodes contained mainly neutral sugars (58.7%) with considerable amounts of uronic acid (32.2%) and a small amount of proteins (9.1%). Component sugars of exo-biopolymer consisted of mainly arabinose, galactose, glucose, mannose, and xylose (0.95:0.81:0.96:1.00:0.39, respectively). When the exo-biopolymer was treated with $NaIO_4, NaClO_2$, and pronase, the $NaClO_2$ treatment and pronase digestion had little effect, whereas $NaIO_4$ oxidation significantly decreased the macrophage stimulating activity (47.6% reduction at $100\mug/ml$). Therefore, the carbohydrate moiety in exo-biopolymer from L. edodes plays an important role in the expression of the macrophage stimulating activity.

Augmentation of Macrophage Cytotoxicity and NO Production by Pedunculagin (Pedunculagin의 Macrophage에 대한 항암활성 및 Nitric Oxide 생성)

  • 이도익;김형근;이민원;최영욱;김하형;김은주
    • YAKHAK HOEJI
    • /
    • v.44 no.2
    • /
    • pp.175-181
    • /
    • 2000
  • Pedunculagin is an ellagitannin purified from Alnus hirsuta var. microphylla, Betulaceae. The effects of pedunculagin on the immune system have been characterized to induce enhancement of NK (natural killer) cell cytotoxicities against tumor cells. The present study investigated whether pedunculagin can enhance macrophage cytotoxicity against P8l5 tumor cells. Macrophage cultured with pedunculagin enhanced cytotoxicity in a dose dependent manner In addition, the same treatments increased NO production, which plays important roles in the immune system. liken together these results demonstrate that pedunculagin significantly enhances cytolytic activities of macrophage.

  • PDF

Effect of Genistein on Apoptosis of T Iymphocyte and Phagocytosis of Peritoneal Macrophage (Genistein이 T lymphocyte의 Apoptosis 및 복강 Macrophage의 탐식능에 미치는 영향)

  • 은재순;조선경;이택렬;김대근;오찬호;소준노
    • YAKHAK HOEJI
    • /
    • v.46 no.1
    • /
    • pp.69-74
    • /
    • 2002
  • The effects of genistein on murine thymocytes for inducing apoptotic cell death and phagocytic activity of peritoneal macrophage were studied in vitro. Addition of genistein (10 and 50$\mu$M) to cultured thymocytes from BALB/c mice definitely promoted DNA fragmentation. Also, cytofluorometric analysis of these cells demonstrated a reduction in mitochondrial transmembrane potential ($\Delta$Ψm). But, repeated administration of genistein (1 mg/mouse/day) to mice for 7 days did not cause any detectable DNA fragmentation. Genistein decreased lucigenin chemiluminescence and engulfment of fluorescein-conjugated E. coli particles in peritoneal macrophage. These results suggest that genistein induce an apoptosis of thymocyte via reduction in $\Delta$Ψm and decrease phagocytic activity of peritoneal macrophage in vitro.