• Title, Summary, Keyword: melanin

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The Effect of Sihosogansangagambang (SS) on Melanin Synthesis and gene expression in B16F10 Mouse Melanoma Cell (시호소간산가감방(柴胡疎肝散加減方)이 멜라닌 생성 및 유전자발현에 미치는 영향)

  • Kim, Ju-Young;Lim, Hyun-Jung;Shin, Sun-Mi;Yoo, Dong-Youl
    • The Journal of Korean Obstetrics and Gynecology
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    • v.22 no.1
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    • pp.95-109
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    • 2009
  • Purpose: This study was performed to determine the inhibitory effect of Sihosogansangagambang (SS) on melanin synthesis in B16F10 melanoma cells (B16F10). Methods: The inhibitory effects of Sihosogansangagambang on melanin synthesis were used by in vitro assay. To elucidate inhibitory effects of SS on melanin synthesis, we determined the melanin release in B16F10. And to investigate the mechanism of inhibitory effect of SS, we assessed the gene expression of tyrosinase, TRP-1, TRP-2 and ERK-1 in B16F10. Results: 1. SS decreased the release of melanin in B16F10 melanoma cells. 2. SS inhibited mushroom tyrosinase activity in vitro. 3. SS decreased the expression of tyrosinase, TRP-2 in B16F10 melanoma cells, but did not decreased the expression of TRP-1 in B16F10 melanoma cells. 4. SS decreased the expression of ERK-1 in B16F10 melanoma cells. Conclusion: From these results, it may be suggested that SS is possesed of the antimelanogenetic effects.

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Effect of Rhynchosia Nulubilis Ethanolic Extract on DOPA Oxidation and Melanin Synthesis (서목태 주정 추출물이 DOPA 산화와 멜라닌 합성에 미치는 영향)

  • Kim, JaeRyeon;Kim, Moon-Moo
    • Journal of Life Science
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    • v.28 no.3
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    • pp.331-338
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    • 2018
  • Melanin is a polymer substance that plays an important role in the determination of hair growth and skin color in vivo. However, melanin, which is over-produced by reactive oxygen species, is known to cause stains, freckles, and hypercholesterolemia, which are associated with aging. Previous studies have shown that polyphosphate, one of the components of Rhynchosia Nulubilis, inhibits skin aging induced by ultraviolet rays. The aim of this study is to investigate the direct effect of Rhynchosia Nulubilis ethanolic extract (RNEE) on melanin synthesis. In this study, RNEE showed no antioxidative effects on scavenging activity of DPPH radical in addition to reducing power. The cytotoxicity of RNEE was increased in a dose-dependent manner in an MTT assay. In addition, RNEE increased tyrosinase activity and melanin synthesis in DOPA-oxidation experiments. RNEE did not promote the conversion L-DOPA into melanin in live cells, but melanin production was promoted in the RNEE-treated group after H2O2 pretreatment compared to the control group in which melanin production was reduced by treatment with H2O2. In addition, RNEE increased the expression level of tyrosinase related protein-2 (TRP-2) and increased the expression level of tyrosinase related protein-1 (TRP-1) at a concentration of $16{\mu}g/ml$. In particular, it was found that RNEE increased the expression level of SOD-3, by which superoxide anion is converted to hydrogen peroxide, higher than the control and ${\alpha}$-MSH used as a positive control at a concentration of more than $16{\mu}g/ml$. The results suggest that RNEE can induce melanogenesis related to black hair.

Fungistatic Activity of Kojic Acid Against Human Pathogenic Fungi and Inhibition of Melanin-production in Cryptococcus neoformans

  • Chee, Hee-Youn;Lee, Eun-Hee
    • Mycobiology
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    • v.31 no.4
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    • pp.248-250
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    • 2003
  • Kojic acid was investigated for its antifungal activity against the human pathogenic fungi including Candida albicans, Cryptococcus neoformans and Trichophyton rubrum. For C. albicans, C. neoformans and T. rubrum, the MIC(minimum inhibitory concentration) of kojic acid was 640, 80 and 160 ${\mu}g/ml$, respectively. In C. neoformans, melanin-producing yeast, kojic acid-treated nonmelanized cell was more susceptible to magainin than melanized cell, suggesting melanin give a protective function against microbial peptide.

Effects of Kojic acid, Arbutin and Vitamin C on cell viability and melanin synthesis in B16BL6 cells

  • Park, Yumi;Lee, Jongsung;Park, Junho;Eunsun Jung;Park, Deokhoon
    • Proceedings of the SCSK Conference
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    • pp.163-173
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    • 2003
  • Research objective:To exactly evaluate their functions of kojic acid, arbutin and vitamin C as a whitening agent, we performed experiments to compare their abilities to inhibit melanin synthesis. Experimental methods and techniques: The effects of kojic acid, arbutin and vitamin C on cell viability and melanin synthesis were evaluated by the level of melanin content and the number of viable cells upon treatment of them.

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Terrein, a Melanin Biosynthesis Inhibitor, from Penicillium sp. 20135

  • KIM WON-GON;RYOO IN-JA;PARK SEO-HYOUNG;KIM DONG-SEOK;LEE SANGKU;PARK KYOUNG-CHAN;YOO ICK-DONG
    • Journal of Microbiology and Biotechnology
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    • v.15 no.4
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    • pp.891-894
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    • 2005
  • In the course of screening a melanin biosynthesis inhibitor, terrein, 4,5-dihydroxy-3-propenyl-2-cyclopenten-l-one, was isolated from Penicillium sp. Terrein was found to have a strong inhibitory activity on melanin formation in B 16 melanoma and melanocyte Mel-Ab cells.

Properties and Functions of Melanin Pigment from Klebsiella sp. GSK

  • Sajjan, Shrishailnath S.;Anjaneya, O;Kulkarni, Guruprasad B.;Nayak, Anand S.;Mashetty, Suresh B.;Karegoudar, T.B.
    • Microbiology and Biotechnology Letters
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    • v.41 no.1
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    • pp.60-69
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    • 2013
  • Purified melanin pigment from Klebsiella sp. GSK was characterized by thermogravimetric, differential thermal, X-ray diffraction and elemental analysis. This melanin pigment is structurally amorphous in nature. It is thermally stable up to $300^{\circ}C$ and emits a strong exothermic peak at $700^{\circ}C$. Its carbon, hydrogen and nitrogen composition is 47.9%, 6.9% and 12.0%, respectively. It was used to scavenge metal ions and free radicals. After immobilizing the pigment and using it to adsorb copper and lead ions, the metal ion adsorption capacity was evaluated by atomic absorption spectroscopy (AAS) and the identity of melanin functional groups involved in the binding of metal ions was determined by Fourier transform infrared (FT-IR) spectroscopy. Batch adsorption studies showed that 169 mg/g of copper and 280 mg/g of lead were adsorbed onto melanin-alginate beads. The metal ion adsorption capacity of the melanin-alginate beads was relatively significant compared to alginate beads. The metal ion desorption capacity of HCl was greater (81.5% and 99% for copper and lead, respectively) than that of EDTA (80% and 71% for copper and lead, respectively). The ability of the melanin pigment to scavenge free radicals was evaluated by inhibition of the oxidation of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and was shown to be about 74% and 98%, respectively, compared with standard antioxidants.

Role of Intracellular $Ca^{2+}$ in the Lovastatin-Induced Stimulation of Melanin Synthesis in B16 Melanoma Cells (B16 흑색종세포에서 로바스타틴에 의한 멜라닌 합성 촉진효과에 미치는 세포내 칼슘의 역할)

  • Lee, Yong Soo
    • YAKHAK HOEJI
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    • v.57 no.1
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    • pp.24-31
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    • 2013
  • Although statins, inhibitors of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, have been shown to increase melanin synthesis, the exact mechanism of this action is not fully understood. In this study we investigated the possible involvement of intracellular $Ca^{2+}$ signal in the mechanism of stimulation of melanin synthesis induced by lovastatin in B16 cells. Lovastatin stimulated the production of melanin in a dose-dependent manner in the cells. Treatment with mevalonate, FPP and GGPP, precursors of cholesterol, did not significantly suppress the lovastatin-induced melanin production, suggesting that inhibition of cholesterol synthesis may not be involved in the mechanism of the action of lovastatin. In addition, lovastatin did not significantly alter the cAMP concentration and the stimulated production of melanin by lovastatin was not significantly changed by treatment with H89, a potent inhibitor of protein kinase A, which demonstrates that cAMP pathway may not be involved. However, lovastatin increased intracellular $Ca^{2+}$ concentration in a dose-related fashion. Treatment with EGTA, an extracellular $Ca^{2+}$ chelator did not significantly alter the lovastatin-induced intracellular $Ca^{2+}$ increase and melanin synthesis, whereas intracellular $Ca^{2+}$ reduction with BAPTA/AM and intracellular $Ca^{2+}$ release blockers (dantrolene and TMB-8) completely blunted these actions of lovastatin. Taken together, these results suggest that the intracellular $Ca^{2+}$ release may play an important role in the lovastatin-induced stimulation of melanin synthesis in B16 cells. These results further suggest that lovastatin may be useful for the treatment of hypopigmentation disorders, such as vitiligo.

Icariin promotes melanin synthesis (Icariin의 멜라닌합성 촉진 작용)

  • Cha, Su Bin;Park, Seol A;Kang, Lea Minju;Woo, Won Hong;Mun, Yeun Ja
    • Herbal Formula Science
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    • v.28 no.1
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    • pp.81-90
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    • 2020
  • Objectives : This study was conducted to investigate the effects of major constituents of Epimedium koreanum Nakai (Icariin, epimedium A, epimedium B, and epimedium C) on melanin synthesis. Methods : We measured melanin contents, tyrosinase activity, and expression of Rab27a in B16F10 cells cultured with Epimedium koreanum Nakai ethanol extract (EKN) and their major constituents. After treatment with H89 and dibutyryl cAMP, which inhibit or promote the activation of PKA, we observed changes in melanin synthesis and tyrosinase activity stimulated by EKN. Results : Among them, EKN and icariin enhanced tyrosinase activity and melanin contents. We confirmed that EKN augmented melanin synthesis via cAMP/PKA pathway. Icariin-induced tyrosinase activity and melanin content were attenuated by PKA inhibitor H89, while melanogenic effect of icariin was further augmented by cAMP analog, dbc AMP. However, icariin did not affect the expression of small GTPase Rab27a involved in melanosome transport. Conclusions : These results suggest that icariin promotes melanogenesis through cAMP/PKA pathway but does not affect small GTPase Rab27a.

Effects of Phenylpropanoid Compounds on Melanin Production in B16 Melanoma Cells (B16 Melanoma 세포에서 Phenylprlopanoid 화합물이 Melanin 생성에 미치는 영향)

  • 박영미;윤미연;김경원;조남영;임혜원;이지윤;이진희;김연정;김창종
    • YAKHAK HOEJI
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    • v.47 no.6
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    • pp.398-403
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    • 2003
  • To investigate the relationship between structure and biological activity of phenylpropanoids, we measured effects of phenylpropanoids on anti-oxidant and whitening activity, In DPPH radical scavenging activity, caffeic acid analogues showed the significant anti-oxidant activity. Although phenylpropanoids did not inhibit purified-tyrosinase activity, they significantly inhibited tyrosinase activity and melanin production in MSH-stimulated B16 melanoma cells. However, phenylpropanoids did not affect tyrosinase expression in MSH-stimulated B16 melanoma cells, which suggest that inhibition of MSH-induced melanin production was due to tyrosinase inhibition mediated via other signal pathways but not expression of tyrosinase. Phenylpropanoids also significantly inhibited both hyaluronidase and elastase activity, suggesting that phenylpropanoids may be used as whitening, hydration and anti-wrinkling agents. Hydroxyl residue of aromatic ring in phenylpropanoids plays an important role in anti-oxidant and whitening activity.

Mycelial Melanization of Rhizoctonia solani AG1 Affecting Pathogenicity in Rice

  • Kim, Heung-Tae;Chung, Young-Ryun;Cho, Kwang-Yun
    • The Plant Pathology Journal
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    • v.17 no.4
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    • pp.210-215
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    • 2001
  • The phenotype of Rhizoctonia solani KR-13 was randomly segregated to both melanin-producing (M+) and non-producing (M-) types through successive cultures on PDA. M+type with dark melanin showed strong pathogenicity to rice and self-anastomosis. Meanwhile, M- type with white or less-melanized mycelia showed very weak pathogenicity and non-self-anastomosis. Melanin production of R. solani was affected by incubation temperature in both M+ and M- types, but not by light treatment. The application of tricyclazole, an inhibitor of fungal melanin biosynthesis, showed no controlling effect on R. solani causing rice sheath blight. Results of this study showed that melanization of mycelia of R. solani is an important pathogenicity factor in rice.

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