• Title, Summary, Keyword: miR-454

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MiR-454 Prompts Cell Proliferation of Human Colorectal Cancer Cells by Repressing CYLD Expression

  • Liang, Hong-Liang;Hu, Ai-Ping;Li, Sen-Lin;Xie, Jia-Ping;Ma, Qing-Zhu;Liu, Ji-Yong
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.6
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    • pp.2397-2402
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    • 2015
  • Previous studies have shown that miR-454 plays an important role in a variety of biological processes in various human cancer cells. However, the underlying mechanisms of this microRNA in colorectal cancer (CRC) cells remain largely unknown. In the present study, we investigated the miR-454 role in CRC cell proliferation. We found that miR-454 expression is markedly upregulated in CRC tissues and CRC cells compared with the matched tumor adjacent tissues and the FHC normal colonic cell line. Ectopic expression of miR-454 promoted the proliferation and anchorage-independent growth of CRC cells, whereas inhibition of miR-454 reduced this effect. Bioinformatics analysis further revealed cylindromatosis (CYLD), a putative tumor suppressor as a potential target of miR-454. Data from luciferase reporter assays showed that miR-454 directly binds to the 3'-untranslated region (3'-UTR) of CYLD mRNA and repressed expression at both transcriptional and translational levels. In functional assays, CYLD-silenced in miR-454-in-transfected SW480 cells have positive effect to promote cell proliferation, suggesting that direct CYLD downregulation is required for miR-454-induced CRC cell proliferation. In sum, our data provide compelling evidence that miR-454 functions as an onco-miRNA, playing a crucial role in the promoting cell proliferation in CRC, and its oncogenic effect is mediated chiefly through direct suppression of CYLD expression.

Transforming Growth Factor β1/Smad4 Signaling Affects Osteoclast Differentiation via Regulation of miR-155 Expression

  • Zhao, Hongying;Zhang, Jun;Shao, Haiyu;Liu, Jianwen;Jin, Mengran;Chen, Jinping;Huang, Yazeng
    • Molecules and Cells
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    • v.40 no.3
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    • pp.211-221
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    • 2017
  • Transforming growth factor ${\beta}1$ $(TGF{\beta}1)/Smad4$ signaling plays a pivotal role in maintenance of the dynamic balance between bone formation and resorption. The microRNA miR-155 has been reported to exert a significant role in the differentiation of macrophage and dendritic cells. The goal of this study was to determine whether miR-155 regulates osteoclast differentiation through $TGF{\beta}1/Smad4$ signaling. Here, we present that $TGF{\beta}1$ elevated miR-155 levels during osteoclast differentiation through the stimulation of M-CSF and RANKL. Additionally, we found that silencing Smad4 attenuated the upregulation of miR-155 induced by $TGF{\beta}1$. The results of luciferase reporter experiments and ChIP assays demonstrated that $TGF{\beta}1$ promoted the binding of Smad4 to the miR-155 promoter at a site located in 454 bp from the transcription start site in vivo, further verifying that miR-155 is a transcriptional target of the $TGF{\beta}1/Smad4$ pathway. Subsequently, TRAP staining and qRT-PCR analysis revealed that silencing Smad4 impaired the $TGF{\beta}1$-mediated inhibition on osteoclast differentiation. Finally, we found that miR-155 may target SOCS1 and MITF to suppress osteoclast differentiation. Taken together, we provide the first evidence that $TGF{\beta}1/Smad4$ signaling affects osteoclast differentiation by regulation of miR-155 expression and the use of miR-155 as a potential therapeutic target for osteoclast-related diseases shows great promise.

The correlation among the oral & facial states and the gummy smile in female college students (일부 여대생의 구강 및 안모상태와 치은노출(Gummy smile)과의 상관성)

  • So, Mi-Hyun
    • Journal of Korean society of Dental Hygiene
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    • v.12 no.2
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    • pp.345-353
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    • 2012
  • Objectives : The author has studied about correlation of gingival exposure upon smiling and oral facial status that reduce facial aesthetic. Methods : The subjects in this study are 91 female vulunteers who were in aged $21.4{\pm}1.89$ in Suwon. Objectives should be normal oral and facial status without the prosthodontic, orthodontic appliance or conqenital missing tooth, and agree to be examined the oral status and impression taking. 1.Measure the length of gingival exposure upon smiling. 2.Measure of the size on central incisor. 3.Measure of Facial. SPSS(SPSS 10.0 for windows, SPSS Inc, Chicago, USA) was utilized for calculating the correlation coefficient between gingival exposure upon smiling and facial status. Regression analysis was calculated in order to predict the R square for gingival exposure upon smiling. Results : 1.Correlation coefficient between the gingival exposure and length of maxillary central incisor was calculated as reversed correlation(r=-.302, p<0.01), and between the gingival exposure and the ratio of the length of central incisor/width of central incisor was revealed as reversed correlation(r=-.250, p<0.05) on smiling. 2.There was correlation between the gingival exposure and the facial height(r=.351, p<0.01), the lower facial height(r=.454, p<0.01) and the upper lip height(r=.274, p<0.01) upon smiling. 3.There was correlation between the gingival exposure and the ratio of the facial height/facial width(r=.358, p<0.05), the ratio of the upper facial height/facial width(r=.214, p<0.05), and the ratio of the lower facial height/facial height(r=.383, p<0.01) upon smiling. 4.The equation of the regression analysis for gingival exposure upon smiling could be estimated as gingival exposure upon smiling=-5.139+.279${\times}$lower facial height-.615${\times}$maxillary central incisal length-.05${\times}$nasolabial angle. Conclusions : Considering these results, it recommended that treatment planning should be designed in consideration of such factors as the length of maxillary central incisor, facial height, upper lip height and lower facial height, in order to promote the easthetic problems of face on smiling.

Optical Properties as Process Condition of Color Conversion Lens Using Low-softening Point Glass for White LED (백색 LED용 저 연화점 유리를 이용한 색 변환 렌즈의 제조 조건에 따른 광 특성)

  • Chae, Yoo-Jin;Lee, Mi-Jai;Hwang, Jong-Hee;Lim, Tae-Young;Kim, Jin-Ho;Jeong, Hee-Suk;Lee, Young-Sik;Kim, Deug-Joong
    • Journal of the Korean Ceramic Society
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    • v.50 no.6
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    • pp.454-459
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    • 2013
  • Recently, remote phosphors have been reported for application to white LEDs to provide enhanced phosphor efficiency compared with conventional phosphor-based white LEDs. In this study, a remote phosphor was produced by coating via screen printing on a glass substrate with different numbers of phosphor coating. The paste consists of phosphor, lowest softening glass frits, and organic binders. The remote phosphor could be well controlled by varying the phosphor content rated paste. After mounting the remote phosphor on top of a blue LED chip, CCT, CRI, and luminance efficiency were measured and values of 5300 K, 62, and 117 lm/W were respectively obtained in the 80 wt% phosphor with 3 coating layers sintered at $800^{\circ}C$.

Relationship between SNS addiction proneness and interpersonal satisfaction among undergraduate students (대학생들의 SNS중독경향성과 대인관계 만족도의 상관관계)

  • Kim, So-Yeon;Park, Mi-Ji;Park, Bu-Kyung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.4
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    • pp.454-462
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    • 2018
  • This study was conducted to examine SNS addiction proneness and interpersonal satisfaction among undergraduate students and the relationships between these two variables, as well as to establish baseline data for appropriate intervention of SNS addiction prevention. The participants of this study were 316 undergraduate students in D and K city, and data were collected between June 30 and July 30, 2017. Data were collected by a self-administered online survey and analyzed by descriptive statistics, t-tests, and Pearson's correlation coefficients using SPSS. The results showed that SNS addiction proneness and interpersonal satisfaction were negatively correlated (r=-0.57, p<0.01), indicating students with higher SNS addiction had lower interpersonal satisfaction. There were no significant differences in SNS addiction proneness and interpersonal satisfaction by gender (t=0.05, p=0.963), number of SNS networks (t=0.66, p=0.513), or number of SNS-only networks (t=-1.24, p=0.216). Students who used SNS for data collection showed significantly higher interpersonal satisfaction (t=3.02, p=0.030); however, there was no significant differences in SNS addiction proneness among purposes for using SNS (t=0.39, p=.759). The results of this study will be useful baseline data for developing an intervention to improve interpersonal satisfaction and prevent SNS addiction among undergraduate students.

Posttraumatic Stress by Work in Firefighters (소방공무원의 직무별 외상 후 스트레스)

  • Baek, Mi-Lye
    • Journal of Korean Society of Hazard Mitigation
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    • v.9 no.3
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    • pp.59-65
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    • 2009
  • To investigate a posttraumatic stress, social support and work burden and to identify high risk group and related factors which were exerted influence on posttraumatic stress of firefighters. Data were gathered from total 264 firefighters consisting of 85 fire distinguishers & rescue workers, 96 emergency medical personnel and 83 in ambulance & fire truck drivers in C province and were analyzed 22 items by IES-R, 20 work burden items by Choi(2000), 8 social support items by Oh(2006) using SPSSWIN 14.0 program. Posttraumatic stress of general characteristics by work was showed significant difference in age, marriage status, education, position, office duration, mobilization frequency and experience of traumatic events. Posttraumatic stress were showed significant difference in 3 groups and fire distinguishers & rescue workers is the highest group. Posttraumatic stress was correlated with work burden(r=.317, p<0.01) for fire distinguishers & rescue workers, social support(r=-.331, p<0.01) and work burden(r=.522 p<0.001) for emergency medical personnel and work burden(r=.454, p<0.01) for ambulance & fire truck drivers. The high risk groups are fire distinguishers & rescue workers(60%), emergency medical personnel(55.2%)and ambulance & fire truck drivers(45.8%). Related factors are the experience seeing victim's danger for fire distinguishers & rescue workers(odds ratio=1.216, 95% confidence interval:1.068-1.383), and are work burden(odds ratio=1.100, 95% confidence interval:1.043-1.159) and office duration(odds ratio=1.010, 95% confidence interval 1.001-1.018) for emergency medical personnel, and are the experience seeing victim's danger(odds ratio=1.178, 95% confidence interval:1.010-1.373), age(odds ratio=1.129, 95%confidence interval:1.020-1.249), work burden(odds ratio=1.103, 95% confidence interval:1.034-1.177) for ambulance & fire truck drivers.

Antioxidative and Antigenotoxic Activity of Extracts from Rhododendron mucromulatum Turcz. Flowers (진달래 꽃 추출물의 항산화 및 항유전독성 활성)

  • Lee, Bo-Bae;Chun, Ji-Hae;Lee, Suck-Hee;Park, Hae-Ryong;Kim, Jung-Mi;Park, Eun-Ju;Lee, Seung-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.12
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    • pp.1628-1632
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    • 2007
  • The present study describes the preliminary evaluation of antioxidant activities and antigenotoxic effect of Rhododendron mucromulatum Turcz. flowers (RMF). The samples were prepared by extracting RMF with four different solvents (methanol, ethanol, acetone, and water), and antioxidant properties were evaluated by determining total phenolic contents (TPC), DPPH radical scavenging activity (RSA), and reducing power (RP). Water extract showed the highest total phenol content (328.1 mg/g gallic acid equivalents). Acetone extract showed the most potent RSA and RP. The $IC_{50}$ for RSA and RP in the acetone extract were 78 mg/mL and 454 mg/mL, respectively. The 200 mM $H_2O_2$ induced DNA damage, measured by Comet assay, was inhibited with water, methanol and acetone extract in dose dependent manner in human leukocytes. The inhibition rates were 42, 62, and 52% at the concentrations of 50 mg/mL of water, methanol and acetone extracts, respectively. These results suggest that R. mucromulatum Turcz. has significant antioxidative activity and protective effect against oxidative DNA damage.

Effect of pH on soil bacterial diversity

  • Cho, Sun-Ja;Kim, Mi-Hee;Lee, Young-Ok
    • Journal of Ecology and Environment
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    • v.40 no.1
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    • pp.75-83
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    • 2016
  • Background: In order to evaluate the effect of pH, known as a critical factor for shaping the biogeographical microbial patterns in the studies by others, on the bacterial diversity, we selected two sites in a similar geographical location (site 1; north latitude 35.3, longitude 127.8, site 2; north latitude 35.2, longitude 129.2) and compared their soil bacterial diversity between them. The mountain soil at site 1 (Jiri National Park) represented naturally acidic but almost pollution free (pH 5.2) and that at site 2 was neutral but exposed to the pollutants due to the suburban location of a big city (pH 7.7). Methods: Metagenomic DNAs from soil bacteria were extracted and amplified by PCR with 27F/518R primers and pyrosequenced using Roche 454 GS FLX Titanium. Results: Bacterial phyla retrieved from the soil at site 1 were more diverse than those at site 2, and their bacterial compositions were quite different: Almost half of the phyla at site 1 were Proteobacteria (49 %), and the remaining phyla were attributed to 10 other phyla. By contrast, in the soil at site 2, four main phyla (Actinobacteria, Bacteroidetes, Proteobacteria, and Cyanobacteria) composed 94 %; the remainder was attributed to two other phyla. Furthermore, when bacterial composition was examined on the order level, only two Burkholderiales and Rhizobiales were found at both sites. So depending on pH, the bacterial community in soil at site 1 differed from that at site 2, and although the acidic soil of site 1 represented a non-optimal pH for bacterial growth, the bacterial diversity, evenness, and richness at this site were higher than those found in the neutral pH soil at site 2. Conclusions: These results and the indices regarding diversity, richness, and evenness examined in this study indicate that pH alone might not play a main role for bacterial diversity in soil.

Comparison of Physicochemical Properties of Meat and Viscera with Respect to the Age of Abalone (Haliotis discus hannai) (전복(Haliotis discus hannai)의 연령에 따른 육과 내장의 이화학적 특성 비교)

  • Lee, Young-Jae;Park, Jeong-Wook;Park, In-Bae;Shin, Gung-Won;Jo, Yeong-Cheol;Koh, So-Mi;Kang, Seong-Gook;Kim, Jeong-Mok;Kim, Hae-Seop
    • Korean Journal of Food Preservation
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    • v.16 no.6
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    • pp.849-860
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    • 2009
  • We compared the physicochemical properties of meat and viscera with regard to the age of abalone (1-, 2-, 3- and 4-year-old abalone). With respect to total amino acids, the level was highest in 1-year-old abalone, at $19,046.00{\pm}548.53\;mg%$, whereas 4-year-old abalone had the lowest value of $15,770.44{\pm}454.19\;mg%$. In the viscera, 3-year-old abalone had the greatest level of total amino acids, at $16,575.10{\pm}477.37\;mg%$, whereas 1-year-old abalone had the lowest, at $14,947.26{\pm}430.48\;mg%$. The level of total free amino acids in meat tended to increase with age whereas that of the viscera fell. The level of polyunsaturated acids decreased with age in meat. The concentration of chondroitin sulfate in both meat and viscera tended to increase with age. This was especially noticeable in meat. The level in 1-year-old abalone, $9.03{\pm}0.23%$ (w/w), rose to $14.17{\pm}0.33%$ in 4-year-old abalone, with statistical significance. On the other hand, the collagen level in both meat and viscera decreased with age. Again, this was particularly noticeable in meat, where the concentration in 1-year-old abalone, $199.70{\pm}5.00\;mg/g$, fell remarkably to the value of $47.86{\pm}1.10\;mg/g$ in 4-year-old abalone. We have thus provided basic data for research on abalone.

Isolation and characterization of lactic acid bacteria for use as silage additives (사일리지 제조를 위한 유산균 탐색 및 특성연구)

  • Ro, Yu-Mi;Lee, Gwan-Hyeong;Park, InCheol;Kim, Wan-Gyu;Han, Byeong-Hak;You, Jaehong;Ahn, Jae-Hyung
    • Korean Journal of Microbiology
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    • v.52 no.4
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    • pp.444-454
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    • 2016
  • Sixteen lactic acid bacterial strains were isolated from silage and cow dung samples, and characterized to identify their potential as silage additives. They were identified as the members of the genera Lactobacillus, Enterococcus, and Weissella, and clustered into nine groups based on the sequences of the genes for 16S rRNA, RNA polymerase alpha subunit, 60-kDa heat shock protein, and phenylalanyl-tRNA synthase alpha subunit. Among them, the three strains which were genetically similar to L. plantarum showed the fastest growth and pH decrease in MRS and rye extract media, the highest numbers of available carbohydrates, and the widest ranges of pH, temperature, and salinity for growth. In addition, they showed no amplified DNA products in the PCR examination targeting the genes for the production of biogenic amines, and the MRS media where they had been cultured showed relatively high inhibition effect against the growth of silage-spoiling microorganisms, including fungi, yeast, and clostridia. The results suggest that these strains are good candidates for silage additives. However, the rye extract media where the lactic acid bacteria had been cultured had no effect on or stimulated the growth of the silage-spoiling microorganisms, and the causes must be established for the practical use of the lactic acid bacteria as silage additives.