• Title, Summary, Keyword: morphometrical analysis

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Morphometrical analysis of chicken Cryptosporidium on electron microscopy (닭 크립토스포리디움의 형태계측을 위한 전자현미경적 연구)

  • Park, Nam-yong;Kim, Young-seop;Chung, Chi-young;Cho, Kyoung-oh;Park, Young-seok;Lee, Bong-joo;Park, Hyung-seon
    • Korean Journal of Veterinary Research
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    • v.39 no.3
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    • pp.575-582
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    • 1999
  • Morphometrical analysis of chicken Cryptosporidium baileyi in various stages of life cycle in the bursa of Fabricius were carried out by electron microscope to establish a differential point for identification of C baileyi. By avidin-biotin complex method, protozoans of the bursa of Fabricius were identified Cryptosporidium spp. The size and area on each developmental stages of C baileyi, as measured by Morphomat 10 attached to electron microscope were as follows. Trophozoites' size with range of $3.21{\pm}0.70{\times}2.49{\pm}0.59{\mu}m$, area with range of $118.82{\pm}41.92{\mu}m^2$; meronts' size $3.99{\pm}1.07{\times}2.96{\pm}0.52{\mu}m$, area $210.11{\pm}57.11{\mu}m^2$; merozoites' size $1.98{\pm}0.43{\times}0.60{\pm}0.18{\mu}m$, area $24.10{\pm}5.97{\mu}m^2$; microgametes' size $1.36{\pm}0.83{\times}0.50{\pm}0.23{\mu}m$, area $20.23{\pm}6.73{\mu}m^2$; macrogametes' size $4.57{\pm}0.65{\times}4.02{\pm}0.55{\mu}m$, area $258.37{\pm}51.83{\mu}m^2$; oocytes' size $4.39{\pm}0.56{\times}3.44{\pm}0.50{\mu}m$, area $187.21{\pm}62.68{\mu}m^2$. In conclusion, the size and area on each developmental stages of Cryptosporidium baileyi is different from that of other Gryptosporidia spp. It suggests, with considering tissue tropism and life cycle, morphometrical analysis can be quite a good way to identify C baileyi.

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BrdU에 의한 DNA

  • 손우찬;김형진;이영순
    • Toxicological Research
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    • v.7 no.1
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    • pp.83-92
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    • 1991
  • Complexities of testis structure and function are emphasized in morphometrical and genotoxic evaluation by statistical analysis. F-344 rats were treated with azinphos methyl, cyclophosphomide, and dichlorvos. And Brdu was injected with intrapertionially before sacrifice. The existence and degree of DNA damage were measured by Brdu labeling index which represented relative amount of Brdu incorporated in DNA, morphometric change was evaluated by the relative length of tubular diameter in circular seminiferous tubules and the number of spermatogonia per Sertoli cell in stage IX seminiferous tubules.

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Morphological and physiological comparison between triploid and diploid Pacific Oyster, Crassostrea gigas (삼배체와 이배체 굴, Crassostrea gigas의 형태 및 생리학적 특성 비교)

  • Kim, Su Kyoung;Shim, Na Young;Lee, Won Young;Choi, Min Seop;Choi, Eun Hee;Lim, Hyun Jeong
    • The Korean Journal of Malacology
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    • v.29 no.3
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    • pp.217-223
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    • 2013
  • The morphological relations and physiological characteristics of the triploid and diploid oysters (Crassostrea gigas) in Taean area, west coast of Korea, were investigated from May 2012 to April 2013. Mophometric analysis indicated that the triploid oysters have the same shell length to shell height ratio but higher shell depth to shell height ratio than diploids. Consistent with morphological characteristics, triploid oysters showed greater values of fatness, condition index and RNA/DNA ratio during the period of experiment. The DNA concentration in adductor muscle and mantle of triploid were either lower or equal to the nucleic acids of diploid. However, RNA/DNA ratio were significantly higher than diploid. It appears that RNA/DNA ratio could be a useful indicator of health condition of triploid and diploid oysters when taken in correlation with the morphological indices.

Morphometrical Analysis of Developing Renal Tubular and Glomerular Cells in Rabbit (토끼의 발생기 뇨세관과 사구체 세포들의 형태계측학적인 분석)

  • 정호중;양영철;배기원
    • Journal of Life Science
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    • v.8 no.4
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    • pp.449-456
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    • 1998
  • Using morphometric technique, the present study was made of the kidney of rabbit embryo in the 13 days of the gestation. Their section areas of the renal tubules and the glomerular cells were compared with the those of adult rabbit, 1. In the developing renal tubule, the tubular section areas were larger than that of the adult, but the nuclear section areas of tubular calls were smaller than the adult. The microvilli of their cell surface were poorly developed, and their cytoplasm contained well developed mitochondria with euchromatic nucleus. 2. In the developing glomeruli. the nuclear section area of the cells of the parietal layer, the podocytes, and the endothelial cells were similar size, but their nuclei were larger than those of the adult. The cytopalsm of the podocytes contained well-developed rough endoplasmic reticulum, Golgi apparatus, and round mitochondria.

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Altered Electrophysiological Properties of Coronary Artery in Iso-prenaline-Induced Cardiac Hypertrophy

  • Kim, Na-Ri;Han, Jin;Kim, Eui-Yong
    • The Korean Journal of Physiology and Pharmacology
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    • v.5 no.5
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    • pp.413-421
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    • 2001
  • An impaired smooth muscle cell (SMC) relaxation of coronary artery by alteration of $K^+$ channels would be the most potential explanation for reduced coronary reserve in left ventricular hypertrophy (LVH), however, this possibility has not been investigated. We performed morphometrical analysis of the coronary artery under electron microscopy and measured $Ca^{2+}-activated\;K\;(K_{Ca})$ currents and delayed rectifier K $(K_{dr})$ currents by whole-cell and inside-out patch-clamp technique in single coronary arterial SMCs from rabbits subjected to isoprenaline-induced cardiac hypertrophy. Coronary arterial SMCs underwent significant changes in ultrastructure. The unitary current amplitude and the open-state probability of $K_{Ca}$ channel were significantly reduced in hypertrophy without open-time and closed-time kinetic. The concentration-response curve of $K_{Ca}$ channel to $Ca^{2+}$ is shifted to the right in hypertrophy. The reduction in the mean single channel current and increase in the open channel noise of $K_{Ca}$ channel by TEA were more sensitive in hypertrophy. $K_{dr}$ current density is significantly reduced in hypertrophy without activation and inactivation kinetics. The sensitivity of $K_{dr}$ current on 4-AP is significantly increased in hypertrophy. This is the first study to report evidence for alterations of $K_{Ca}$ channels and $K_{dr}$ channels in coronary SMCs with LVH. The findings may provide some insight into mechanism of the reduced coronary reserve in LVH.

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Effects of Isokinetic Exercise on the Composition of Muscle Fibers in Rats Lower Limb (등속성 운동이 흰쥐 하퇴의 근섬유조성에 미치는 영향)

  • Lee, Yong-Deok;Cho, Hyun-Gug;Kim, Han-Soo;Park, Youn-Ki;Suh, Tae-Soo
    • The Journal of Korean Physical Therapy
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    • v.3 no.1
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    • pp.75-90
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    • 1991
  • This study is to characterize the effects of swimming exercise on the composition of muscle fibers in rats lower limb tissue. By comparing the exercised group (4, 8 weeks) with the control group, we analyzed the difference histologically, histochemically and morphometrically. The obtained results are as follows ; 1. In the histological view, the distance between the individual muscle fibers and the muscle bundles in exercised group was shorter than that of control group. The irregular shape of sarcolemma in exercised group was observed. 2, In the histochemical view(NADH-TR. and Myosin ATPase reaction analysis), the increased populations of red and intermediate fibers were observed, whereas the white fiber was decreased. 3. In the morphometrical view, the red and intermediate fibers of tibialis anterior and gastrocnemius in exercised group were quantitatively increased, compared with control group. However, the white fiber and connective tissue in exercised group were decreased.

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Histomorphometry and stability analysis of early loaded implants with two different surface conditions in beagle dogs

  • Kim, Dong-Seok;Kim, Dae-Gon;Park, Chan-Jin;Cho, Lee-Ra
    • The Journal of Advanced Prosthodontics
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    • v.1 no.1
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    • pp.10-18
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    • 2009
  • STATEMENT OF PROBLEM. Despite an improved bone reactions of Mg-incorporated implants in the animals, little yet has been carried out by the experimental investigations in functional loading conditions. PURPOSE. This study investigated the clinical and histologic parameters of osseointegrated Mg-incorporated implants in early loading conditions. MATERIAL AND METHODS. A total of 36 solid screw implants(diameter 3.75 mm, length 10 mm) were placed in the mandibles of 6 beagle dogs. Test groups included 18 Mg-incorporated implants. Turned titanium implants served as control. Gold crowns were inserted 4 weeks after implant placement and the dogs were immediately put on a food diet. Implants were observed for 10 weeks after loading. Radiographic assessments and stability tests were performed at the time of fixture installation, $2^{nd}$ stage surgery, 4 weeks after loading, and 10 weeks after loading. Histological observations and morphometrical measurements were also performed. RESULTS. Of 36 implants, 33 displayed no discernible mobility, corresponding to successful clinical function. There was no statistically significant difference between test implants and controls in marginal bone levels(P=.46) and RFA values. The mean BIC % in the Mg-implants was $54.5{\pm}8.4%$. The mean BIC % in the turned implant was $45.3{\pm}12.2%$. These differences between the Mg-implant and control implant were statistically significant(P=.005). CONCLUSIONS. The anodized, Mg-incorporated implant demonstrated significantly more bone-to-implant contact(BIC) in early loading conditions. CLINICAL IMPLICATIONS. The results of this study in beagle dogs suggest the possibility of achieving predictable stability of early loaded free-standing dental implants with Mg-incorporated surface.

Morphogenetical characteristics of Korean wild rat (Rattus norvegicus) (한국 야생 랫드의 형태 유전학적 특성)

  • Seong, Je-Kyung;Yun, Young-min;Park, Ji-young;Oh, Seung-hyun;Do, Seon-gil;Jin, Hee-kyung;Hyun, Byung-hwa;Suh, Jun-gyo;Oh, Yang-seok
    • Korean Journal of Veterinary Research
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    • v.39 no.5
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    • pp.896-907
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    • 1999
  • The morphometrical characteristics such as external measurements and mandible size assessment in mice and rats have to be highly heritable and sufficiently variable between strains in order to calculate a strain specific profiles. The coat color of Korean wild rats were observed and morphometric analysis of external measurements were carried out on Korean wild rats compared to laboratory strains in order to clarify the genetic characteristics of Korean wild rats and to establish background data as a domestication of Korean wild rats for new laboratory strain. Korean wild rats were captured from Chunchon and Hoengsong. 4 inbred and 1 outbred strains of rats were used in this study for the comparison of genetic characteristic of Korean wild rats. Total body length, head length, tail length, hind foot length and ear length were measured and then statistical analysis were carried out by discrimiant analysis. The coat color of Korean wild rat showed golden white in ventral portion and dark agouti in dorsal portion. Korean wild rats could be distinguished from the other laboratory strains distinctly by morphogenetical analysis. There was significant variations among Korean wild rat compared to those of the other laboratory strains of rat. This study may provide that Korean wild rats have a unique genetic characterization compared to those of other inbred strains of rats based on morphogenetical characteristics by external measurements.

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Morphometrical characteristics of River Meandering (하천 사행의 계량형태학적 특성)

  • 이재우;이원환
    • Water for future
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    • v.14 no.1
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    • pp.39-49
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    • 1981
  • The purpose of this study is to examine the meander charactericstics for the rivers in Korea..In this study, the new characteristics factors of meander are proposed, and the relationships among the factors proposed in this study and the existing factors are derived. An attempt is made to find considerable relation among meander characteristics, but width and meander belt did not show any defined trend and considerable scatter of points was observed. Relationships among the meander length, belt and flowrate, etc., which are factors of meander characteristics, are analyzed the 67 rivers above 30km in length. Channel shape factor which is the ratio of the length from the starting point to the end to the channel lenght, tortuosity which is the ratio of the curved channel length against the channel length are suggested for a new characteristics factor of meander. They are well correlated with channel length, Horton's shape facotr and meander gradient, consequently have to be important measures of river meander. The result of the detailed comparison and the analysis of degree of sinuosity, velocity and water surface slop are brought light on the fact show that the curved reach is morestable than the straight one. The ratio of the meander length to the meander belt and its accumulative frequency showed excellent correlationship when plotted on the semi-log paper. The results of regression analysis of meander belt and meander length show linear for the South Han river branches and power curve for the Geum river and the Nakdong river branches.

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Histomorphometry and Stability Analysis of Loaded Implants with two Different Surface Conditions in Beagle Dogs (하중을 가한 두 가지 표면의 임플란트에 관한 조직형태학적 분석 및 안정성 분석 (비글견을 이용한 연구))

  • Kim, Sang-Mi;Kim, Dae-Gon;Cho, Lee-Ra;Park, Chan-Jin
    • Journal of Dental Rehabilitation and Applied Science
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    • v.24 no.4
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    • pp.337-349
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    • 2008
  • Despite an improved bone reactions of Mg-incorporated implants in the animals, little yet has been carried out by the experimental investigations in functional loading conditions. This study investigated the clinical and histologic parameters of osseointegrated Mg-incorporated implants in delayed loading conditions. A total of 36 solid screw implants (diameter 3.75 mm, length 10mm) were placed in the mandibles of 6 beagle dogs. Test groups included 18 Mg-incorporated implants. Turned titanium Implants served as control. Gold crowns were inserted 3 months. Radiographic assessments and stabilitytests were performed at the time of fixture installation, $2^{nd}$ stage surgery, 1 and 3 months after loading. Histological observations and morphometrical measurements were also performed. Of 36 implants, 32 displayed no discernible mobility, corresponding to successful clinical function. There was no statistically significant difference between test implants and controls in marginal bone levels (p=0.413) and RFA values. The mean BIC % in the Mg-implants was $54.4{\pm}20.2%$. The mean BIC % in the turned implant was $48.9{\pm}8.0%$. These differences between the Mg-implant and control implant were not statistically significant (P=0.264). In the limitation of this study, bone-to-implant contact (BIC) and bone area of Mg-incorporated oxidized implant were similar to machine-turned implant. The stability analysis showed no significantly different ISQ values and marginal bone loss between two groups. Considering time-dependent bone responses of Mg-implant, it seems that Mg-implants enhanced bone responses in early loading conditions and osseointegrated similarly to cp Ti implants in delayed loading conditions. However, further investigations are necessary to obtain long-term bone response of the Mg-implant in human.