• Title, Summary, Keyword: natural antimicrobial

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A Study on the Pharmacetical and Chemical Characteristics of Natural Artemisia Extract (천연 쑥 추출물의 약리 및 화학적 특성 연구)

  • Sung, Ki-Chun
    • Journal of the Korean Applied Science and Technology
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    • v.26 no.1
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    • pp.51-59
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    • 2009
  • Natural Artemisia extraction was extracted from Artemisia component using diethyl ether as a solvent, and we tested various pharmacetical and chemical characteristics of this extract. Characteristic experiments to use natural Artemisia extract tested antimicrobial experiment using microbe in pharmacetical material, and tested dye experiment using fiber in chemical material. From the result of characteristics experiment, some conclusions are obtained as follow. From the result of extraction experiment, it obtained about 10.4%-Artemisia extraction ratio as semi-solid state, and after dried in freezing from Artemisia extract of semi-solid state, it obtained about 10%-Artemisia extraction ratio as solid state of dark blue-green color. From result of antimicrobial experiment of Artemisia extract, number of staphylococcus aureus (ATCC-01) and aspergillus niger (ATCC-02) in microbe decreased more and more according to time passage. This phenomenon showed that Artemisia extract influences to antimicrobial effect. From the result of dye experiment of Artemisia extract, it appeared in direction of dark blue-green color after dyed to use cotton and silk with fiber to control in pH 7.5. Specially the result which confirmed dye of fiber with optical electron microscope (OEM), we could know that it appears darker silk than cotton.

A Study on the Pharmaceutical and Chemical Characteristics of Natural Grape Extract (천연 포도 추출물의 약리 및 화학적 특성 연구)

  • Sung, Ki-Chun
    • Journal of the Korean Applied Science and Technology
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    • v.26 no.3
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    • pp.341-349
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    • 2009
  • Natural grape extraction was extracted from grape component using diethyl ether as a solvent, and we tested various pharmaceutical and chemical characteristics of this extract. Characteristic experiments to use natural grape extract tested antimicrobial experiment using microbe in pharmaceutical material, and tested dye experiment using fiber in chemical material. From the result of characteristics experiment, some conclusions are obtained as follow. From the result of characteristics experiment, it obtained about 7.5%-grape extraction ratio as semi-solid state, and after dried in freezing from grape extract of semi-solid state, it obtained about 10%-grape extraction ratio as solid state of dark purple color. From result of antimicrobial experiment of grape extract, number of staphylococcus aureus (KCMC-01) and aspergillus niger (KCMC-02) in microbe decreased more and more according to time passage. This phenomenon showed that grape extract influences to antimicrobial effect. From the result of dye experiment of grape extract, it appeared in direction of dark purple color after dyed to use cotton and silk with fiber to control in pH 7.5. Specially the result which confirmed dye of fiber with optical electron microscope(OEM), we could know that it appears darker silk than cotton.

Antioxidative and Antimicrobial Effects of Nut Species (Nut류의 항산화 및 항균효과)

  • Lee, Hye-Won;Choi, Moo-Young;Cha, Bae-Cheon
    • Korean Journal of Pharmacognosy
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    • v.29 no.1
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    • pp.28-34
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    • 1998
  • It has been known that diseases related with aging or cancer result, at least in part, from free radicals, and antioxidants may reduce or prevent the abnormalities associated with free radical formation via its scavenging action. Thus, this study was carried out to investigate the antioxidative and antimicrobial activities of nut species for the purpose of developing antioxidant from natural products. Antioxidant activities of four kinds of nuts such as chestnut, groundnut, walnut and acorn were examined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The extracts from chestnut hull and acorn barnyard millet showed strong antioxidative activities among 10 samples tested. Because of their highest antioxidative activity among 10 samples, radical scavenging effects of 4 different extract compartments (Hexane, EtOAc, BuOH and $H_2O$ extracts) from chestnut hull and acorn barnyard millet, were further examined by DPPH method. EtOAc and BuOH extracts exhibited antioxidative activities similar to those of natural, tocopherol or synthetic antioxidants, BHA. These findings demonstrates that major fraction of the antioxidative activity of chestnut hull or acorn barnyard millet was the EtOAc and BuOH extract compartments. However, antimicrobial activities against food-related bacteria and yeasts was relatively weak.

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Antimicrobial Activity of Some Food Additives against Bacteria and Saccharomyces cerevisiae (몇종의 세균과 Saccharomyces cerevisiae에 대한 식품첨가물의 향균 특성)

  • Lee, Jong-Soo;Oh, Jun-Sei;Kim, Na-Mi;Keum, Jong-Hwa;Lee, Suk-Kun
    • The Journal of Natural Sciences
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    • v.8 no.2
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    • pp.49-55
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    • 1996
  • In order to survey the safety of some food additives, antimicrobial activity of acidulants, stabilizers, antioxidants, natural coloring materials and bleaching agents against 5 strains of bacteria and Sacch. cerevisiae were investigated by dilution method and minimal inhibitory concentration(MIC) method. Malic acid as acidulants displayed the effective antimicrobial activity in vitro against P. aeruginosa and its MIC is 0.05%. Alginic acid and pectin as stabilizer also displayed strong antimicrobial activity against B. subtilis, E. coli and P. aeruginosa, and tannin(antioxidants) and $NaHSO_3$ displayed antimicrobial activity against all bacteria tested. However gums(Arabia, Xanthan, Gua) and natural coloring materials(Hongwha Yellow, Red powder-N) were not affected to growth of bacteria and Sacch. cerevisiae.

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Investigation of Optimum Extracting Condition and Antimicrobial Activity of the Extract from the Root Bark of Morus alba (상백피 추출물의 향균력 및 최적추출조건 검토)

  • 박욱연;김영목;김신희;장동석
    • Journal of Food Hygiene and Safety
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    • v.10 no.3
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    • pp.139-145
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    • 1995
  • In order to develop a natural food preservative, the root bark of Morus alba was extracted with several solvents, and then antimicrobial activity was investigated. The optimum extracting condition for the antimicrobial substance from the sample, minimum inhibitory concentration (MIC) of the extracted substance against microorganisms were also examined. The antimicrobial activity of the ethanol extract form the sample was stronger than those of the extracts by the other solvents such as water, methanol. ethyl acetate and acetone. The optimum extracting condition for antimicrobial substance from the sample was shaking extraction twice for 5 hours at room temperature in case of 7 times of absolute ethanol added to the crushed root bark of Morus alba. The ethanol extract from the root bark of Morus alba had strong B. cereus, L. monocytogenes and S. aureus. Especially, Bacillus species was the most susceptible to the extracted substance. The ethanol extract showed antimicrobial activity against Gram negative bacteria(MIC, 160~1600 ug/ml) and yeasts(MIC, 1600 ug/ml) such as C. albicans and S. acidifaeciens. The extract also showed growth inhibition against molds such as A. niger, A. parasiticus, A versicolar and T. viride.

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Development of Antimicrobial Dye for Natural Dyeing using Natural Substances (천연물을 이용한 항균성 염료의 개발)

  • Yu, Young-Eun;Park, Eun-Young;Jung, Dae-Hwa;Byun, Sung-Hui;Kim, Sang-Chan;Park, Sung-Min
    • Microbiology and Biotechnology Letters
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    • v.38 no.1
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    • pp.32-39
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    • 2010
  • This study was carried out to investigate the usefulness of the natural antimicrobial substances for natural dyeing. Antimicrobial activity of natural substances, extracted by water, ethanol, methanol and ethyl acetate, were shown over 20 mm by Rheum palmatum, Caesalpinia sappan, Prunus mume, Schizandra chinensis, Rhus javanica, and Coptis japonica. Especially, water and methanol extract of C. japonica were shown strong antimicrobial activity against all investigated strains. Minimum inhibition concentration of C. japonica water extract was investigated 110 mg/mL for Staphylococcus aureus ATCC 6538. Investigated natural substances were not only shown strong antimicrobial activity for natural dyeing but also might be used other industries.

Study of Natural Preservative System Using the Mixture of Scutellariae radix, Acacia nilotica and Citrus reticulata Extracted from Polyhydric Alcohols (다가알코올로부터 추출된 황금, 아카시아, 한라봉 추출물의 천연 방부시스템 연구)

  • Park, Sung-Min;Lee, Kyeong-Ah;Yun, Mi-Young;Kim, Young-Jae;Lee, Sang-Hwa
    • KSBB Journal
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    • v.26 no.6
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    • pp.533-537
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    • 2011
  • The aim of this study was to develop a new natural preservative system to improve the weak points of natural polyhydric alcohols together with the efficiency of natural plants as a preservative. Polyhydric alcohols (glyceryl caprylate and ethylhexylglycerin) and antimicrobial plants (S. radix, A. nilotica and C. reticulata) were tested using the disc diffusion method and minimum inhibitory concentration (MIC) method for their antimicrobial activity against the common poultry pathogens, respectively. A study of the preservative efficacy of the cosmetic formulations containing the optimized preservative system demonstrated sufficient preservative efficacy against bacteria and eukaryotic test microbes. These results suggest that the natural preservative system including polyhydric alcohol extracts containing natural plants could be incorporated in cosmetic formulations.

Purification of Antimicrobial Compounds and Antimicrobial Effects of Schima wallichii subsp. liukiuensis against Candida sp. (Schima wallichii subsp. liukiuensis의 Candida종에 대한 항균효과 및 항균물질의 분리정제)

  • Choi, Mynug-Suk;Shin, Kuem;Yang, Jae-Kyung;Ahan, Jin-Kwon;Kwon, Oh-Woong;Lee, Yi-Young
    • KSBB Journal
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    • v.16 no.3
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    • pp.269-273
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    • 2001
  • To develop natural antimicrobial substances from Theaceae, Schima wallichii subsp. liukiuensis was selected from 218 woody plants, and antimicrobial compounds against bacteria, fungi, and yeast were isolated. The antimicrobial activity of ethanol extracts proved higher than those of other organic solvents. The antimicrobial activity of S. liukiuensis extract showed no differences in sesonal variation, but, that of plant part was high in bark at autumn. An antimicrobial substance was isolated from the extract of Schima using column chromatography packed with silica gel and sephadex LH-20, and then a purified antimicrobial substance (Compound I) was obtained using HPLC analysis. The Compound I in the analysis of UV, IR, and GC-MS presumed a triterpene or steroidal saponin, ${\alpha}$-sitisterol as aglycon combined three sugars. The minimal inhibitory concentration (MIC) of the Compound I against a bacteria, fungi, and yeast were 1.25 g/L, 5.0 g/L, and 0.040 g/L, respectively. This is much lower than the MIC of hinokitiol, an natural antimicrobial compound used commercially, which suggests that Compound I could be developed as a natural preservative and pharmaceuticals.

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Antimicrobial Activity of Allyl Derivate in Multi-purpose Solution as Natural Preservative (다목적용액에서 천연보존제로서의 Allyl 유도체의 항균활성)

  • Lee, Ji-Young;Suh, Seung-Kyo;Sung, Duk-Yong;Youk, Do-Jin;Lee, Koon-Ja;Chung, Jae-Yong
    • Journal of Korean Ophthalmic Optics Society
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    • v.14 no.3
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    • pp.95-102
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    • 2009
  • Purpose: The purpose of this study is to investigate antimicrobial activity of ally derivative of garlic against eye diseases, and to get basic data for an application development of the derivative, as a natural preservative, to multi-purpose solution (MPS). Methods: Antimicrobial activity of allyl derivative of garlic, diallyl sulfide (DAS), against Pseudomonas aeruginosa (P. aeruginosa) and Staphyloccus aereus (S. aereus) was determined. After DAS treatment in MPS of no microbial activity, its growth inhibition effect to bacteria was also determined. Results: DAS concentrations of $IC_{50}$ against P. aeruginosa and S. aereus were found when both bacteria were treated with DAS concentration of 0.25% and 0.64%, respectively. At MPS test, a product from Company A did not show any effects against both bacteria, but one from Company B showed antimicrobial activity against both bacteria. Antimicrobial activity test of the product from Company B in MPS did not showed any significant difference on both gram-negative and gram-positive bacteria. For a trial application of antimicrobial activity of DAS as a natural preservative, DAS was added to MPS of Company A which did not show any antimicrobial activity at treated concentration. Addition of DAS into MPS of Company A resulted in antimicrobial activity of DAS, which suggested a possible application of DAS as a germicide for contact lenses. Conclusions: This study showed allyl derivative of garlic has antimicrobial activity against both P. aeruginosa and against S. aereus, but more effects on P. aeruginosa. The result suggests that allyl derivative derived from garlic can be developed to a preservative of MPS as a natural substance.

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Antimicrobial Effects of Lonicera japonica against Gram Positive and Gram Negative Anaerobic Bacteria

  • Rhee, Ki-Hyeong;Lee, Keyong-Ho
    • Natural Product Sciences
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    • v.17 no.1
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    • pp.23-25
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    • 2011
  • It has been shown that the butanol extract of Lonicera japonica has antimicrobial and other potentially useful biological activities. The purpose of this study was to determine the in vitro activity of Lonicera japonica compared to other antimicrobial agents against anaerobic bacteria. Specifically, the in vitro activity of the butanol extract was investigated against 104 clinical isolates of anaerobic bacteria using an agar dilution method and the results were compared to erythromycin, cefoxitin, imipenem, clindamycin, and metronidazole. It was found that Lonicera japonica and imipenem were the most active antimicrobial agents tested.