• Title, Summary, Keyword: natural killer cell

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A Study on Relationship of Symptom Distress and Natural Killer Cell Cytotoxicity in Breast Cancer Patients (치료 중인 유방암 환자의 신체적 증상과 자연살해세포 활성도의 관계)

  • Chae, Young-Ran
    • Journal of Korean Biological Nursing Science
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    • v.4 no.2
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    • pp.69-77
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    • 2002
  • The purpose of this study was to identify the relationship of symptom distress and natural killer cell cytotoxicity in breast cancer patients who had been radiation therapy and/or chemotherapy after surgery. Symptom distress measured by modified Lee's(1994) physical symptom questionnaire. For measuring the natural killer cell cytotoxic activity. 8ml to 10ml blood was collected from the subjects. Mononuclear cell was isolated by centrifuge of the blood and cultured by putting $Cr^{51}$, and reacted with target cell, K562 cell. Amount of $Cr^{51}$ was measured, and %lysis was calculated. The results were as follows. 1) Symptom distress score was 42.18, which is moderate symptom distress. 2) Natural killer cell cytotoxic activities were 42.18%lysis(effector : target cell ratio=100 : 1) and 28.05%lysis(effector : target cell ratio=50 : 1). 3) Correlation coefficients of symptom distress and natural killer cell cytotoxic activity were $-.134{\sim}-.461$. Though significant correlation was not found between total score of symptom distress and natural killer cell cytotoxic activity, 3('pain' 'feel hot on radiation site' and 'difficulty in breathing') of 19 symptom distress items and natural killer cell cytotoxic activity showed significant negative correlation(p<.05). These findings suggest that 1) breast cancer patients who had been radiation therapy and/or chemotherapy after surgery have moderate symptom distress and decreased natural killer cell cytotoxic activity. 2) The symptom distress was not related to natural killer cell cytotoxic activity.

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Relationships between Radiation-induced Prostaglandin E2 and Natural Killer Cell Activity in Mice (방사선조사(放射線照査)에 의한 Prostaglandin E2 및 자연살해세포(自然殺害細胞) 활성도(活性度)의 변화(變化))

  • Kim, Sung-ho
    • Korean Journal of Veterinary Research
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    • v.27 no.2
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    • pp.185-189
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    • 1987
  • The number of splenic lymphocyte, serum prostaglandin $E_2$ level and natural killer cell activity were assayed after single whole body irradiation of a sublethal dose of $^{60}Co-{\gamma}$ ray to C57BL/6J mice. With a view to knowing the relationships between radiation induced prostaglandin $E_2$ level and the normal natural killer cell activity after natural killer cell-target cell conjugation, The change of normal natural killer cell activity were measured by administration of prostaglandin $E_2$ containing serum from irradiated mice. The results were summarized as follows; 1. The total number of splenic lymphocyte was significantly decreased by irradiation and the number was not affected by indometacin, prostaglandin synthesis inhibitor, treatment. 2. Serum prostaglandin $E_2$ level was increased in irradiated mice, but indometacin treated mice group showed low level of prostaglandin $E_2$. 3. In the case of irradiated mice, natural killer cell activity was not shown any difference between irradiated group and indometacin combined group. But when natural killer cell-target cell conjugations were exposed to the serum of each group during cytotoxic activity assay, whereas the normal natural killer cell activity was significantly decreased by treatment of serum from irradiated mice, the activity was not changed by treatment of indometacin pretreated mice serum. This result indicated that the prostaglandin $E_2$ induced by the radiation inhibited the post-target binding cytolytic process of natural killer activity.

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Involvement of the Enhancement of Natural Killer Cell Activity on the Anti-Cancer Effect of Red Gingseng during Rat Hepatocarcinogenesis (랫드의 간압발생과정에서 홍삼의 항암효과와 자연살해세포의)

  • 강경선;이영순
    • Toxicological Research
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    • v.13 no.1_2
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    • pp.23-27
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    • 1997
  • This study was performed to examine the anti-cancer effect of Red Ginseng in the DENGalN-PH-induced hepatic tumor model system in rats. One hundred of male SPF Sprague-Dawley rats(6weeks old) were randomly divided into five groups. Rats in groups 1, 2, 3, and 4 were administered to diethylnitrosamine intraperitoneally 200 mg/kg body weight for the caner initiation. Rats in group 5 were given to saline as a control. On two weeks after cancer initiation, rats in groups 1 and 3 were fed on diet containing 0.01% of acethylaminofiuorene(AAF) which is strong cancer-promotor for 6 weeks, while rats in groups 2 and 4 were fed on water containing 0.05% of phenobarbital which is weak cancer.promotor for 6 weeks. Rats in groups 1 and 2 were treated with diet containing 3% of Red Ginseng for six weeks(from 9th week till 15th week after cancer initiation). Rats in all groups were necropsied time-sequencially at 8, 15, and 36 weeks. The hepatic lesions of rat treated with carcinogens expressed glutathione S-transferase placental form(GST-P) at 8 week. The GST-P positive foci of rats treated with AAF were larger than that of any other rats, while the GST-P positive foci of rats treated with AAF and red ginseng were significantly decreased. This anti-cancer effect of Red ginseng might be involved in the enhacement of natural killer cell activity. To know whether there is direct relationship between Red Ginseng and natural killer cell activity, the activity of natural killer cell was examined after treatment AAF, AAF+Red ginseng and Red ginseng only, respectively. Comparing with natural killer cell activity in AAF-treated group, natural killer cell activity was significantly activated in AAF+ Red ginseng-treated group. This indicated that Red ginseng might enhance natural killer activity after treatment carcinogen in rats. These results suggested that Red ginseng might have a cancer prevention ability by promoting natural killer cell activity during hepatocarclnogenesis.

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Effect of Exercise on Natural Killer Cell Cytotoxic Activity in Breast Cancer Patients (운동 프로그램이 유방암 환자의 자연살해세포 활성에 미치는 효과)

  • Chae, Young-Ran;Choe, Myoung-Ae;Kim, Mi-Jung
    • Journal of Korean Biological Nursing Science
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    • v.4 no.2
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    • pp.59-68
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    • 2002
  • The purpose of this study was to determine the effect of exercise program on natural killer cell cytotoxic activity(NKCA) in breast cancer patients who had been radiation therapy after surgery. The subjects in the experimental group consisted of 11 breast cancer patients, while the subjects in the control group consisted of 15. Subjects in the experimental group participated in exercise program for 8 weeks. Exercise program consisted of shoulder stretching, arm weight training and treadmill walking exercise. They started to exercise on treadmill for 20 minutes per day, 3 times a week at 40% of maximum heart rate, and increased intensity and duration of exercise so that they were running 30 minutes/day at 60% of maximum heart rate from the 3rd week to the 8th week. Natural killer cell cytotoxic activity were determined before and after the exercise program. For measuring the natural killer cell cytotoxic activity, 8ml to 10ml blood was collected from the subjects. Mononuclear cell was isolated by centrifuge of the blood and cultured by putting $Cr^{51}$, and reacted with target cell, K562 cell. Baseline demographic and medical data were compared between groups with the Fisher's exact test and Mann-Whitney U test. For effects of the exercise program, repeated measures ANOVA was used. The result was as follows; Natural killer cell cytotoxic activity(NKCA) in experimental group comparing with control group significantly increased after the exercise program in case of effector cell : target cell ratio is 100 : 1(p<0.05). The above result suggest that the exercise program for breast cancer patients undergoing radiation therapy after breast surgery may increase the natural killer cell cytotoxic activity.

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Synergistic Effect of Natural Killer Cells and Bee Venom on Inhibition of NCI-H157 Cell Growth

  • Sung, Hee Jin;Song, Ho Sueb
    • Journal of Acupuncture Research
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    • v.33 no.1
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    • pp.47-56
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    • 2016
  • Objectives : This study examined the effects of Bee venom on apoptosis in NCI-H157 human lung cancer cells and for promoting the apoptosis effects of Natural killer cell. Methods : Bee venom and Natural killer-92 cells were cultured either separately from or together with NCI-H157 cells for 24 hours. To figure out whether Bee venom enhances the cytotoxic effect of Natural Killer-92 cells, a cell viability assay was conducted. To observe the changes in Death receptors, apoptotic regulatory proteins and Nuclear $Factor-{\kappa}B$, western blot analysis was conducted. To observe the effect of Bee venom through an extrinsic mechanism, a transfection assay was conducted. Results : 1. Natural killer-92 cells and Bee venom significantly inhibited the growth of NCI-H157 cells and co-culture had more inhibitory effect than the separate culture. 2. Expressions of Fas, DR3, DR6, Bax, caspase-3, caspase-8, cleaved caspase-3, cleaved caspase-8 were increased, and expressions of Bcl-2 and cIAP were decreased. More efficacy was observed in co-culture than in separate culture. 3. Nuclear $Factor-{\kappa}B$ activation was clearly decreased. And co-culture showed much less activation than separate culture. 4. As a result of treatment for DR-siRNA, the reduced cell viability of NCI-H157 cells and the activity of Nuclear $Factor-{\kappa}B$ were increased. With this, it can be seen that Bee venom and Natural killer-92 cells have an effect on the cancer cells through the extrinsic mechanism. Conclusion : Bee venom is effective in inhibiting the growth of human lung cancer cells. Furthermore Bee venom effectively enhances the functions of Natural killer cells.

Isolation and morphological characterization of natural killer cell in the sprague-dawley(SD) rats (Sprague-dawley(SD) 랫드에서 natural killer cell의 분리 ·동정 및 형태적 특징)

  • Kang, Kyung-sun;Lee, Yong-soon
    • Korean Journal of Veterinary Research
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    • v.32 no.2
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    • pp.245-250
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    • 1992
  • This study was performed to demonstrate the presence of large granular lymphocyte(LGL) in Sprague-Dawley(SD) rats and morphologically observe NK cell and also establish the method of isolation of natural killer cell in SD rats. By percoll discontinuous density gradients centrifugation, highly enriched LGL population were shown to fraction 2(border line between 44.2% and 50.8%). LGL were shown to bind selectively to YAC1 mouse lymphoma cell. This fraction expressed very high NK cell cytolysis. Therefore, we thought that LGL have NK activity in SD rats. The Morphology of rat LGL is very similar to that of human LGL. These cells have an eccentric kidney-shaped nucleus. Their most distinctive feature was their cytoplasmic azurophilic granules. Another distinguishing feature of rat LGL was their high cytoplasmic : nuclear ratio. It was concluded that LGL played a role part in mediating natural killer activity in this species.

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The Effect of Ginseng on the Immunotoxicity of Benzo(a)pyrene (Benzo(a)pyrene의 면역독성에 미치는 인삼의 영향)

  • 안영근;김주영;신하경;정종갑
    • Environmental health and toxicology
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    • v.1 no.1
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    • pp.47-54
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    • 1986
  • The immunopotentiating effect of ethanol extract, butanol fraction and petroleum ether extract of Panax ginseng on the immunotoxicity of benzo(a)pyrene were investigated in mice. A single administration of benzo(a)pyrene induced an apparent but relatively transient reduction in HY titer, Arthus reaction, delayed type hypersensitivity, rosette forming cell and natural killer cell activity Ethanol extract very significantly restored HY titer, Arthus reaction. RFC and natural killer cell activity. Butanol fraction have no effect. But petroleum ether extract very significantly restored humoral and cellular immune response and especially natural killer cell activity.

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The Effect of Korean Ginseng on the Immunotoxicity of Mitomycin C (Mitomycin C의 면역독성에 미치는 고려인삼의 영향)

  • 안영근;김주영;정종갑;김정훈;구자돈
    • YAKHAK HOEJI
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    • v.31 no.6
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    • pp.355-360
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    • 1987
  • The immunopotenciating effects of ethanol extract, butanol fraction and petroleum ether extract of Korean Ginseng on the immunotoxicity of mitomycin C were investigated in mice. A single administration of mitomycin C induced an apparent but relatively transient reduction of spleen weight, hemagglutination titer, Arthus reaction, RFC and natural killer cell activity. Ethanol extract of Ginseng significantly restored spleen weight, HA titer, RFC and natural killer cell activity. Butanol fraction of Ginseng significantly restored HA titer, RFC and natural killer cell activity. Petroleum ether extract of Ginseng very significantly restored spleen weight, Arthus reaction, DTH, RFC and natural killer cell activity.

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The changes of plasma prostaglandin E2 level and natural killer cell activity in EL-4 leukemia cells bearing mice (EL-4 암세포주(癌細胞株) 이식(移植)마우스에서의 혈중(血中) prostaglandin E2 농도(濃度) 및 자연살해세포(自然殺害細胞) 활성도(活性度)의 변화(變化))

  • Kim, Sung-ho
    • Korean Journal of Veterinary Research
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    • v.29 no.4
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    • pp.469-474
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    • 1989
  • The changes of plasma prostaglandin $E_2$ level, natural killer cell activity and tumor cell growth were assayed after transplantation of EL-4 leukemia cells in C57BL/6 mice. The results were summarized as follows; 1. Plasma prostaglandin $E_2$ level was increased in EL-4 bearing mice, but indomethacin treated mice group showed low level. 2. The tumor-derived prostaglandin $E_2$ inhibited the post-target binding cytolytic process of natural killer activity. 3. Indomethacin inhibited the growth of prostaglandin secreting EL-4 solid tumor.

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Natural killer T cell and pathophysiology of asthma

  • Jang, Gwang Cheon
    • Clinical and Experimental Pediatrics
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    • v.53 no.2
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    • pp.136-145
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    • 2010
  • Natural killer T (NKT) cell is a special type of T lymphocytes that has both receptor of natural killer (NK) cell (NK1.1, CD161c) and T cell (TCR) and express a conserved or invariant T cell receptor called $V{\alpha}14J{\alpha}18$ in mice or Va24 in humans. Invariant NKT (iNKT) cell recognizes lipid antigen presented by CD1d molecules. Marine-sponge-derived glycolipid, ${\alpha}-galactosylceremide$ (${\alpha}-GalCer$), binds CD1d at the cell surface of antigen-presenting cells and is presented to iNKT cells. Within hours, iNKT cells become activated and start to secrete Interleukin-4 and $interferon-{\gamma}$. NKT cell prevents autoimmune diseases, such as type 1 diabetes, experimental allergic encephalomyelitis, systemic lupus erythematous, inflammatory colitis, and Graves' thyroiditis, by activation with ${\alpha}-GalCer$. In addition, NKT cell is associated with infectious diseases by mycobacteria, leshmania, and virus. Moreover NKT cell is associated with asthma, especially CD4+ iNKT cells. In this review, I will discuss the characteristics of NKT cell and the association with inflammatory diseases, especially asthma.