• Title, Summary, Keyword: neonicotinoids

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Indirect Bacterial Effect Enhanced Less Recovery of Neonicotinoids by Improved Activities of White-Rot Fungus Phlebia brevispora

  • Harry-Asobara, Joy L.;Kamei, Ichiro
    • Journal of Microbiology and Biotechnology
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    • v.29 no.5
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    • pp.809-812
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    • 2019
  • Bacterial strains that improve mycelial morphology and growth of white-rot fungi in liquid medium could enhance the impact of white-rot fungi towards lesser recovery of neonicotinoids when cocultured. This was demonstrated by the recovery of clothianidin and acetamiprid from cocultures of the white-rot fungus Phlebia brevispora strains with two mycelial-growth-promoting bacteria, Enterobacter sp. TN3W-14 and Pseudomonas sp. TN3W-8. Clothianidin recovery from cocultures of white-rot fungi and bacteria was over 40% lower than that from axenic microbial cultures and mixed-bacterial cultures. About 20% less acetamiprid was equally recovered from both TMIC33929+TN3W-14 cocultures and mixed-bacterial cultures than from axenic fungal and bacterial cultures.

Avoidance Behavior of Honey bee, Apis mellifera from Commonly used Fungicides, Acaricides and Insecticides in Apple Orchards

  • Kang, Moonsu;Jung, Chuleui
    • Journal of Apiculture
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    • v.32 no.4
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    • pp.295-302
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    • 2017
  • Avoidance behavior is an important life history strategy to survive hazardous environment. The experiment was conducted to detect the avoidance tendency of the honeybee Apis mellifera against commonly used pesticides in apple production. Choice test given only 50% sucrose solution and pesticide-mixed sucrose solution as food estimated the avoidance in laboratory. Most of the acaricides and fungicides tested were shown avoided. Among insecticides, honeybee showed strong avoidance to cyhexatine, carbosulfan and fenpyroximate but low to diflubenzuron, tebufenpyrad, and acrinathrin. Avoidance behavior to neonicotinoid insecticides showed bifurcated; highly avoided from thiacloprid, acetamiprid while less avoided from imidacloprid, thiamethoxam and dinotefuran. From the field study, abamectin, fenthion, amitraz and acequinocyl showed highly avoided while fungicide of fenarimol, acaricides of acrinathrin and phosphamidon, IGR insecticide of diflubenzuron, neonicotinoid insecticide of imidacloprid, and carbamate insecticide of carbaryl showed less avoidance in the field. These results partly explained high bee poisoning from carbaryl in apple flowering period, and neonicotinoids during season.

Insecticidal Activity of Japanese Pine Sawyer (Monochamus alternatus) and Toxicity Test of Honeybee (Apis mellifera) using 5 Kinds of Neonicotinoids (5종의 네오니코티노이드계 약제에 대한 솔수염하늘소의 살충활성과 꿀벌독성)

  • Cho, Woo Seong;Jeong, Dae-Hoon;Lee, Jae Seon;Kim, Hyun Kyung;Seo, Sang-Tae;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.21 no.1
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    • pp.33-41
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    • 2017
  • Susceptibility and persistence of Japanese pine sawyer, Monochamus alternatus adults, and acute contact toxicity, foliage contact toxicity, and residual toxicity to honeybee, Apis mellifera were evaluated by using 5 kinds of neonicotinoid pesticides. All neonicotinoids pesticides showed over 80% mortality 48 hour after body spray treatment. However, only 2 kinds of acetamiprid ME and acetamiprid+buprofezin showed over 80% mortality in the twig dipping treatment. All of the five neonicotinoides pesticides used in M. alternatus adults showed residual effect of 60-80% mortality, but the efficacy decreased rapidly after 1 day of treatment. Acetamiprid ME showed the lowest toxicity in the acute and foliage contact toxicity test of A. mellifera. The residual toxicity of leaves on A. mellifera was very low in acetamiprid ME and acetamiprid+buprofezin. However, the residual toxicity of all pesticides treatments decreased to 10-30% after 1 day of treatment and further decreased with time. These results indicate that acetamiprid ME among five neonicotinoid agents showed a high insecticidal activity in the M. alternatus adults, while it exhibited relatively low contact and residual toxicity in the A. mellifera. It is thought that acetamiprid ME can be effectively used for the control of the M. alternatus adults as vector of pine wilt disease.

Do neonicotinoid insecticides impaired olfactory learning behavior in Apis mellifera?

  • Imran, Muhammad;Sheikh, Umer Ayyaz Aslam;Nasir, Muhammad;Ghaffar, Muhammad Abdul;Tamkeen, Ansa;Iqbal, Muhammad Aamir
    • International Journal of Industrial Entomology
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    • v.38 no.1
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    • pp.1-5
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    • 2019
  • Bee's population is declining and disappearing at alarming rate. There are many factors responsible for declining the population of bees including diseases, natural enemies, environmental conditions and pesticides. Insecticides play its role dramatically for their population decline and neonicotinoid insecticides are critically important due to their wide application for pest control. Keeping in view of above problem, effect of neonicotinoid insecticides on olfactory learning behavior in Apis mellifera was observed using Proboscis Extension Reflex (PER) method. In this method, bees were harnessed in centrifuges tubes and feed on insecticides mixed sugar solution after three hours hunger. Bees were checked by feeding on non-treated sugar solution to observe PER response. Minimum proboscis extension was observed for acetamiprid and imidacloprid with 26% and 20% respectively at their recommend field doses while it was maximum for dinotefuran and thiamethoxam with 73% and 60% respectively. Only 40% bees showed response when exposed at 1/10 concentration of field dose for imidacloprid and the least at 1/100 of field dose. At control (Sugar solution) about 90% bees showed PER response. Among these neonicotinoid insecticides tested, imidacloprid and acetamiprid were the most damaging which impaired the olfactory learning performance in Apis mellifera.

Insecticide Targets: Learning to Keep Up with Resistance and Changing Concepts of Safety

  • Casida, John E.;Quistad, Gary B.
    • Journal of Applied Biological Chemistry
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    • v.43 no.4
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    • pp.185-191
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    • 2000
  • Pest insect control is dependent on about 200 insecticides that work by relatively few mechanisms. The targets they disrupt are mostly involved in the nervous system, respiratory chain, growth and development, or the gut. The major nerve targets are: acetylcholinesterase for the organophosphates and methylcarbamates; the nicotinic acetylcholine receptor for the neonicotinoids; the $\gamma$-aminobutyric acid receptor for several chlorinated hydrocarbons and fipronil; the voltage-gated sodium channel for DDT and pyrethroids. Selection of resistant strains often confers cross-resistance to some or all other insecticides working at the same site. The toxicological properties of different compounds acting on the same target are increasingly considered together, summating the risk even though the compounds are of quite diverse chemical types. Continuing attention is also being given to secondary targets not involved in the primary mechanism of toxicity but instead in side effects that must be considered in the overall safety evaluation. Research on insecticide targets is important in learning to keep up with resistance and changing concepts and policies on safety. These relationships are illustrated by recent studies in the Environmental Chemistry and Toxicology Laboratory of the University of California at Berkeley.

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Resistance development and cross-resistance of green peach aphid, Myzus persicae (Homoptera : Aphididae), to imidacloprid (Imidacloprid에 대한 복숭아혹진딧물의 저항성 발달 및 교차저항성)

  • Choi, Byeong-Ryeol;Lee, Si-Woo;Yoo, Jai-Ki
    • The Korean Journal of Pesticide Science
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    • v.6 no.4
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    • pp.264-270
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    • 2002
  • Studies on the resistance monitoring of green peach ahpid, Myzus persicae, its development pattern by artificial selection with imidacloprid and cross-resistance were carried out to develope resistance management strategy. Resistance ratios of M. persicae collected at Hwachon and Dunnae among 5 locations in alpine cultivation area appeared to be high as 37.2 and 16.5, respectively. Resistance of aphid to imidacloprid developed slowly up to 20 time selection, and after that it grew quickly. Imidacloprid-resistant aphid strain showed low cross-resistance ratios(<10) to most of organophosphates, carbamates, and mixed insecticides except pirimicarb(487.8), but high ratios to acetamiprid(143.0) which is one of the neonicotinoids like imidacloprid, and pyrethroids such as deltamethrin(14.9), flucythrinate(12.9) and halothrin(15.9).

Susceptibility commercially of North American planthopper, Metcalfa pruinosa to commercially registered insecticides in Korea (국내시판 살충제에 대한 미국선녀벌레의 감수성)

  • Ahn, Ki-Su;Lee, Gwan-Seok;Lee, Kyeong-Hee;Song, Myung-Kyu;Lim, Sang-Cheol;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.15 no.3
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    • pp.329-334
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    • 2011
  • Insecticidal activity of 31 registered insecticides was tested against Metcalfa pruinosa adults. All experiments were conducted at the recommended concentration (ppm) of each insecticide. Among them, 16 insecticides from organophosphates (dichlorvos, fenitrothion, fenthion, methidathion, phenthoate), carbamates (methomyl), pyrethroids (${\alpha}$-cypemethrin, deltamethrin, fenpropathrin, ${\gamma}$-cyhalothrin), neonicotinoids (acetamiprid, clothianidin, dinotefuran, imidacloprid, thiamethoxam), and other (endosulfan) showed 100% mortality by spraying on the body of M. pruinosa adults. Dichlorvos, fenitrothion, fenthion, methidathion, phenthoate and endosulfan showed 100% mortality by plant-dipping method. The residual effect was showed 100% mortality in four insecticides (fenitrothion, fenthion, methidathion, phenthoate) at one day after treatment, and three insecticides (fenitrothion, methidathion, phenthoate) were showed the mortality of 90% at three days after treatment.

Monitoring on Insecticide Resistance of Major Insect Pests in Plastic House (시설 재배 작물 주요 해충에 대한 약제저항성 모니터링)

  • Choi, Byeong-Ryeol;Park, Hyung-Man;Yoo, Jai-Ki;Kim, Sun-Gon;Baik, Chai-Hun;Lee, Si-Woo
    • The Korean Journal of Pesticide Science
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    • v.9 no.4
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    • pp.380-390
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    • 2005
  • This study was carried out for looking into the status of susceptibility of vinyl house insect pests to insecticides. The Thrips (Thrips palmi and Frankliniela occidentalis), Mites (Tetranychus urticae), Aphids (Aphis gossypii) and Whitefly (Trialeurodes vaporariorum) were captured at various areas where the host crop was being cultivated and the susceptibility level of each pest insect was investigated. The susceptibility of each pest insect varied by insect species and areas where they were caught. The tested insecticides showed good control effect to palm thrips in 2000, but in 2003 showed decrease of effect to them. Western flower thrips showed low susceptibility to neonicotinoids, imidacloprid and thiamethoxam, but high to chlorfenapyr, spinosad, emamectinbenzoate and fipronil. Antibiotic insecticides, abamectin and milbemectin, and chlorfenapyr were very effective on mite control and dicofol still had good effectiveness to it despite of long year use. No aphid species showed resistance to pyrethroid and carbamate insecticides. Relatively new insecticides such as imidacloprid, spinosad, pymetrozine were effective to whitefly, but not were organophosphates, carbamates and pyrethroids.

Toxicity of Firefly, Luciola lateralis(Coleoptera: Lampyridae) to Commercially Registered Insecticides and Fertilizers (애반딧불이(Luciola lateralis)에 대한 살충제와 비료의 독성평가)

  • Lee, Ki-Yeol;Kim, Young-Ho;Lee, Jae-Wung;Song, Myung-Kyu;Nam, Sang-Ho
    • Korean journal of applied entomology
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    • v.47 no.3
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    • pp.265-272
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    • 2008
  • Toxicity of 10 registered insecticides and 6 fertilizers were tested against $3^{rd}$ larva and adults of Korean firefly, Luciola lateralis Motschulsky(Coleoptera: Lampyridae). All experiments were tested at the recommended concentration of each inescticides by producer. MEP, PAP, Acephate, Fenthion, and Diazinon, which were organophates, a mixtures combined with Burofezin fenobucarb, Cartap buprofezin, and Thiamethoxam(Neonicotinoids), Fipronil(Phenylpyrazoles) showed more 80.0% mortality on larva and adults of L. lateralis. However, tebufenozide(I.G.R) showed low mortality of 33.3%. $LC_{50}$ (ppm) value of Assit, Cartap buprofezin, Fenthion and PAP were showed 1.03 ppm, 1.90 ppm, 10.26 ppm, 0.98 ppm, respectively, against $3^{rd}$ larva of L. lateralis. Effects against eggs showed very high toxicity. Otherwise, tebufenozide(I.G.R) was showed hatchability of 100%. Toxicity of Urea fertilizer, Ammonium sulfate, Potassium chloride, Fused phosphate, Complex fertilizer and Silicate fertilizer were showed the mortality with 27.3%, 56.7%, 73.3%, 0.0%, 0.0%, 0.0%, respectively, when exposed 72 hrs after treatment.

Insecticidal activity of mixed formulation with buprofezin and single formulation without buprofezin against citrus mealbug, Planococcus citri Risso (Hemiptera: Pseudococcidae) (귤가루깍지벌레에 대한 단제 및 buprofezin 혼합제의 살충 활성)

  • Park, Young-Uk;Park, Jun-Won;Lee, Sun-Young;Yun, Seung-Hwan;Koo, Hyun-Na;Kim, Gil-Hah
    • The Korean Journal of Pesticide Science
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    • v.16 no.2
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    • pp.163-170
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    • 2012
  • Nineteen registered insecticides including 8 mixed formulations with buprofezin and 11 single formulations which is not including buprofezin were evaluated by spray application for their toxicity against adult and nymph of citrus mealybug, Planococcus citri. Five mixed formulations with buprofezin such as buprofezin 15EC+acetamiprid 4EC, buprofezin 10SC+clothianidin 3SC, buprofezin 20WP+dinotefuran 15WP, buprofezin 20SC+thiacloprid 5SC, and buprofezin 20SC+thiamethoxam 3.3SC showed high insecticidal activity (>93%) against nymph and adult of P. citri. Insecticidal activities of EPN 45EC, fenitrothion 50EC and methidathion 40EC in organophosphorous group showed 90 to 93% against nymph only. In addition to, insecticidal activities of acetamiprid 8WP, clothianidin 8SC, dinotefuran 20WG and thiamethoxam 10WG in neonicotinoids group showed above 90% against nymph only. In systemic and residual effect, five mixed formulations that was already proved to have high insecticidal activity showed low toxicity with below 60% against $3^{rd}$ instar nymph of P. citri in tomato and rose under greenhouse. Control efficacy of five mixed formulations with buprofezin was above 90%, 80% and 70% at 5 days after treatment (DAT), 10 DAT and 15 DAT, respectively.