• Title, Summary, Keyword: neurotrophic factor

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Neurotrophic Factors and Their Roles (신경영양성 인자와 역할)

  • Kim Sik-Hyun;Nam Ki-Won
    • The Journal of Korean Physical Therapy
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    • v.11 no.2
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    • pp.131-137
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    • 1999
  • Neurotrophic factors control the survival and differentiation in developing neurons, Furthermore, nut evidence suggests that neurotrophic factors promote the axonal growth and synaptic plasticity In the CNS. Research is currently being undertaken in order to determine whether members of the neurotrophic factor family have potential therapeutic roles in preventing and/or reducing the neuronal cell death and atrophy. This review summarizes the current knowledge of characterized neurotrophic factors including NGF, BDNF, NT-3, and NT-4/5.

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In Vitro Neural Cell Differentiation Derived from Human Embryonic Stem Cells: I. Effect of Neurotrophic Factors on Neural Progenitor Cells

  • Kim Eun-Yeong;Jo Hyeon-Jeong;Choe Gyeong-Hui;An So-Yeon;Jeong Gil-Saeng;Park Se-Pil;Im Jin-Ho
    • Proceedings of the KSAR Conference
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    • pp.18-18
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    • 2002
  • This study was to investigate the effect of neurotrophic factors on neural cell differentiation in vitro derived from human embryonic stem (hES, MB03) cells. For neural progenitor cell formation derived from hES cells, we produced embryoid bodies (EB: for 5 days, without mitogen) from hES cells and then neurospheres (for 7 - 10 days, 20 ng/㎖ of bFGF added N2 medium) from EB. And then finally for the differentiation into mature neuron cells, neural progenitor cells were cultured in ⅰ) N2 medium (without bFGF), ⅱ) N2 supplemented with brain derived neurotrophic factor (BDNF, 5ng/㎖) or ⅲ) N2 supplemented with platelet derived growth factor-bb (PDGF-bb, 20ng/㎖) for 2 weeks. (omitted)

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Antidepressant Effects of Pharmacopuncture on Behavior and Brain-Derived Neurotrophic Factor (BDNF) Expression in Chronic Stress Model of Mice

  • Kim, Yunna;Lee, Hwa-Young;Cho, Seung-Hun
    • Journal of Acupuncture & Meridian Studies
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    • v.10 no.6
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    • pp.402-408
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    • 2017
  • Objectives: The present study aimed to investigate the antidepressant effect of the traditional Korean medical pharmacopuncture, Liver Qi Depression (HJ11), in a mouse model of depression induced by exposure to chronic immobilization stress (CIS). Methods: Mice were subjected to 2 hours of immobilization stress daily for 14 days. They were also injected with distilled water (DW) (CIS + DW) or HJ11 at the acupoints HT7, SP6, and GV20 (CIS + HJ11) an hour before stress. The positive control group (CIS + paroxetine) was intraperitoneally injected with paroxetine (10 mg/kg, 14 days). The tail suspension test and the forced swimming test were performed to assess depression-like behaviors. Western blotting was also conducted to seek the change in brain. Results: CIS + DW mice showed significantly longer immobile times in the tail suspension test and forced swimming test than sham mice that did not go through daily restraint. Immobility of CIS + HJ11 and that of CIS + paroxetine mice was significantly decreased compared with immobility of CIS + DW mice. Immunoblotting showed that HJ11 increased the expression of brain-derived neurotrophic factor both in the hippocampus and the amygdala. Conclusion: HJ11 improves depressive-like behaviors in the stress-induced mouse model of depression, and the results indicate that the neuroprotective effect of HJ11, identified by brain-derived neurotrophic factor expression, may play a critical role in its antidepressant effect.

Isolation and Characterization of Brain-Derived Neurotrophic Factor Gene from Flounder (Paralichthys olivaceus)

  • LEE JAE HYUNG;CHOI TAE-JIN;NAM SOO WAN;KIM YOUNG TAE
    • Journal of Microbiology and Biotechnology
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    • v.15 no.4
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    • pp.838-843
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    • 2005
  • Brain-derived neurotrophic factor (BDNF) is a small secretory protein and a member of the nerve growth factor (NGF) gene family. We cloned the flounder BDNF gene from a flounder brain cDNA library. The nucleotide sequence of the cloned gene showed an open reading frame (ORF) consisting of 810 bp, corresponding to 269 amino acid residues. The tissue distribution of flounder BDNF was determined by reverse transcription-polymerase chain reaction (RT-PCR) in brain, embryo, and muscle tissues. To express fBDNF using a eukaryotic expression system, we constructed the vector mpCTV-BDNF containing the fBDNF gene and transformed this vector into Chlorella ellipsoidea. Stable integration of introduced DNA was confirmed by PCR analysis of genomic DNA, and mRNA expression in C. ellipsoidae was confirmed by RT-PCR analysis.

The Effect of Therapeutic Exercise on Brain-Derived Neurotrophic Factor After Global Brain Ischemia in Rats (흰쥐의 전뇌허혈 후 재관류 시 운동치료에 의한 신경영양성인자 발현)

  • Gu, Sang-Hun;Song, Ju-Young;Kown, Young-Shil;Nam, Ki-Won;Song, Ju-Min;Lee, Yun-Seob;Choi, Jin-Ho;Kim, Jin-Sang
    • The Journal of Korean Physical Therapy
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    • v.13 no.2
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    • pp.281-292
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    • 2001
  • This study was performed to investigate the effect of therapeutic exercise on brain-derived neurotrophic factor manifestation after global brain ischemia in rats. Nine rats with global ischemia were divided at random into two group. In the control group, three rats remained in cage. But, in the end, two rats were alive. In the therapeutic exercise group, six rats remained. The five rats of this group was swam for 30 minutes everyday for a week. The brain-derived neurotrophic factor expression was identified from immunohistochemistry. The results of this study were as follows : 1. In the control group, a little expression of brain-derived neurotrophic factor was observed at cortex and hippocampus layer, but cell body and axon was observed obscurely. 2. In the experimental group, a much expression of brain-derived neurotrophic factor was observed at cortex and hippocampus layer, and cell body and axon was observed clearly. In the neurological examination(beam-walking test). experimental group was obtained higher 1.4 points than control group. BDNF expression was increased by swimming for 30 minutes everyday for a week. Therefore, therapeutic exercise contribute to brain plasticity after brain ischemia.

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ROLES OF MAPK PATHWAYS IN GDNF-INDUCED GLIOMA CELL MIGRATION

  • Hyun Song;Chung, Dong-June;Choung, Pill-Hoon;Aree Moon
    • Proceedings of the Korean Society of Toxicology Conference
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    • pp.140-140
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    • 2002
  • Glial cell-derived neurotrophic factor (GDNF) is a potent neurotrophic factor that enhances survival of midbrain doparminergic neuron and is a member of the transforming growth factor-b superfamily. GDNF and its receptors are widely distributed in brain and are believed to be involved in the control of neuron survival, proliferation and differentiation.(omitted)

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The Effects of Regular Taekwondo Exercise on Brain wave activation and Neurotrophic Factors in Undergraduate male students (16주간의 태권도 프로그램이 중년 비만 여성의 뇌신경성장인자 및 인지기능에 미치는 영향)

  • Ha, Min-Seong;Roh, Hee-Tae;Park, Hae-Chan;Cho, Su-Youn
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.2
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    • pp.354-361
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    • 2020
  • The purpose of this study was to investigate the effects of regular taekwondo training on neurotrophic factors and cognitive function in obese middle-aged women. Thirty-three middle-aged women with obesity were selected for this study and randomly assigned into a control group(CG, n=18) and an taekwondo group(TG, n=15). The TG performed taekwondo training 5 times weekly for 16 weeks, while the CG did not exercise training. Serum brain-derived neurotrophic factor(BDNF), vascular endothelial growth factor(VEGF), and insulin-like growth factor-1(IGF-1) levels were analyzed and Stroop Color and Word tests were performed before and after the intervention. The serum BDNF and IGF-1 levels were significantly increased in the TG after the intervention(p<.05). On the other hand, no statistically significant differences were found in the serum VEGF levels, or in the Stroop Color and Word Test scores(p>.05). These results suggest that regular taekwondo training may be affects levels of peripheral neurotrophic factors but not cognitive function in obese middle-aged women.

GDNF Enhances Hs683 Human Glioma Cell Migration: Possible Involvement of MAPKs

  • Song , Hyun;Chung, Dong-June;Choung, Pill-Hoon;Moon , A-Ree
    • Proceedings of the PSK Conference
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    • pp.326.2-327
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    • 2002
  • Glial cell-derived neurotrophic factor (GDNF) is a potent neurotrophic factor that enhances survival of midbrain doparminergic neuron. GDNF and its receptors are widely distributed in brain and are believed to be involved in the control of neuron survival and differentiation. In this study, we examined the effect of GDNF on proliferation and migration of Hs683 human glioma cells. GDNF markedly enhances proliferation and migration of Hs683 cells in a dose-dependent manner. (omitted)

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Involvement of MAPKs in GDNF-induced Proliferation and Migration in Hs683 Glioma Cells

  • Song, Hyun;Moon, A-Ree
    • Proceedings of the PSK Conference
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    • pp.223.2-224
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    • 2003
  • Glial cell-derived neurotrophic factor (GDNF) is a potent neurotrophic factor that enhances survival of midbrain doparminergic neuron. GDNF and its receptors are widely distributed in brain and are believed to be involved in the control of neuron survival and differentiation. GDNF increased proliferation and migration of Hs683 human giloma and C6 rat giloma cells in a dose-dependent manner. (omitted)

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