• Title, Summary, Keyword: niacinamide

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Effects of Edge Activator on the Droplet Size and Skin Permeation of Hydrated Liquid Crystalline Vesicles (Edge Activator가 수화 액정형 베시클의 입자크기와 피부 침투에 미치는 영향)

  • Lee, Seo Young;Lim, Yoon Mi;Jin, Byung Suk
    • Applied Chemistry for Engineering
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    • v.28 no.6
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    • pp.679-684
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    • 2017
  • Hydrated liquid crystalline vesicles incorporating a edge activator, which confers flexibility to the vesicle membranes, were prepared and niacinamide was encapsulated in them. The formation of liquid crystalline phases and their thermal phase transitions were investigated by polarized optical microscopy and differential scanning calorimetry (DSC), respectively. Droplet sizes of the vesicles were reduced to several tens of nanometers by incorporating edge activators, such as sodium deoxycholate, lysolecithin, or polysorbate 80. The amount of niacinamide permeated into a pig skin increased greatly using the hydrated liquid crystalline vesicles compared to the case where niacinamide was applied in an aqueous solution state. The vesicles incorporating 10% sodium deoxycholate increased the amount of niacinamide permeated nearly four times. These results suggest that edge activators are effective in improving the skin permeability of vesicles.

Study on the Adsorption of Pharmaceuticals by Filter aids -Adsorption of caffeine, niacinamide, pyridoxine hydrochloride and thiamine hydrochloride by Celite 545 and Dawsonite-

  • Hyun, Yona-Woo;Min, Shin-Hong;Rhee, Shang-Hi;Kim, Yong-Bae
    • Journal of Pharmaceutical Investigation
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    • v.3 no.4
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    • pp.39-43
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    • 1973
  • 완충액(緩衝液)에서의 caffeine, niacinamide, pyridoxine hydrochloride, thiamine hydrochloride의 여과조제(濾過助劑)에 의(依)한 흡착(吸着)현상을 비교검토(比較檢討)하였다. 실험(實驗)에 사용(使用)한 vitamin 류(類)의 흡착(吸着)은 Langmvir 흡착식(吸着式)의 pattern 에 따랐으며 caffeine 은 거의 흡착(吸着)되지 않았다. 여과조제중(濾過助劑中) Dawsonite는 Celite 545 보다 큰 흡착(吸着)현상을 나타냈으며 pH변화(變化)에 따른 영향(影響)은 niacinamide가 가장 예민하였고 pH의 증가(增加)는 흡착량(吸着量)을 현저히 감소시켰다.

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Niacinamide Protects Skin Cells from Oxidative Stress Induced by Particulate Matter

  • Zhen, Ao Xuan;Piao, Mei Jing;Kang, Kyoung Ah;Fernando, Pincha Devage Sameera Madushan;Kang, Hee Kyoung;Koh, Young Sang;Yi, Joo Mi;Hyun, Jin Won
    • Biomolecules & Therapeutics
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    • v.27 no.6
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    • pp.562-569
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    • 2019
  • Niacinamide (NIA) is a water-soluble vitamin that is widely used in the treatment of skin diseases. Moreover, NIA displays antioxidant effects and helps repair damaged DNA. Recent studies showed that particulate matter 2.5 ($PM_{2.5}$) induced reactive oxygen species (ROS), causing disruption of DNA, lipids, and protein, mitochondrial depolarization, and apoptosis of skin keratinocytes. Here, we investigated the protective effects of NIA on $PM_{2.5}$-induced oxidative stress in human HaCaT keratinocytes. We found that NIA could inhibit the ROS generation induced by $PM_{2.5}$, as well block the $PM_{2.5}$-induced oxidation of molecules, such as lipids, proteins, and DNA. Furthermore, NIA alleviated $PM_{2.5}$-induced accumulation of cellular $Ca^{2+}$, which caused cell membrane depolarization and apoptosis, and reduced the number of apoptotic cells. Collectively, the findings show that NIA can protect keratinocytes from $PM_{2.5}$-induced oxidative stress and cell damage.

A Study on Skin Permeability and Skin Whitening Effect of High Pressure Emulsification Processed Cosmetic (고압유화 공정이 적용된 화장품의 피부흡수 및 피부 미백효과 연구)

  • Jang, Sue Im;Lee, So Mi;Paik, Byung Ryol;Han, Jiyeon;Kim, Eun Joo;Lee, Hae Kwang
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.43 no.3
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    • pp.189-194
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    • 2017
  • Skin has a strong barrier function to protect the body from external harmful factors. This strong barrier function of the skin makes for any effective substance difficult to permeate through the skin. The purpose of this study was to evaluate skin permeability of the reduced particle size by non-invasive method. The particles were prepared by high-pressure emulsification. The niacinamide prepared in a high-pressure emulsification was absorbed 1.56 times higher than the control sample. In addition, it was found from the sixth layer of keratin that was removed by tape-stripping method. This was significantly deeper penetration. The whitening efficacy of the sample containing niacinamide was evaluated before and after 6 weeks of use. The skin color was significantly improved in both pigmented area and non-pigmented area. Through this study, it was possible to visually and quantitatively confirm the permeability confirm the permeability of the reduced particle sample into skin.

Downregulation of $\textrm{NF}_{-{\kappa}}\textrm{B}$ activation in human keratinocytes by melanogenic inhibitors

  • Ahn, Kwang-Seok;Lee, Jinseon;Kim, Yeong-Shik
    • Proceedings of the SCSK Conference
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    • pp.780-803
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    • 2003
  • Exposure of skin cells, particularly keratinocytes to various nuclear factor-kappaB ($\textrm{NF}_{-{\kappa}}\textrm{B}$) activators [e.g. tumor necrosis factor-$\alpha$, interleukin-1, lipopolysaccharides, and ultraviolet light] leads to phosphorylation and degradation of the inhibitory protein, $\textrm{I}_{{\kappa}}\textrm{B}$. Liberated $\textrm{NF}_{-{\kappa}}\textrm{B}$ is translocated into the nucleus where it can change or alter expression of target genes, resulting in the secretion of extracellular signaling molecules including melanotrophic factors affecting melanocyte. In order to demonstrate the possible role of $\textrm{NF}_{-{\kappa}}\textrm{B}$ activation on the synthesis of melanotrophic factors from the keratinocytes, the activities of $\textrm{NF}_{-{\kappa}}\textrm{B}$ induced by melanogenic inhibitors (MIs) were determined in human HaCaT keratinocytes transfected with $\textrm{pNF}_{-{\kappa}}\textrm{B}$-SEAP-NPT plasmid. Transfectant cells released the secretory alkaline phosphatase (SEAP) as a transcription reporter in response to the $\textrm{NF}_{-{\kappa}}\textrm{B}$ activity and contain the neomycin phosphotransferase (NPT) gene for the dominant selection marker for geneticin resistance. MIs such as niacinamide, kojic acid, hydroquinone, resorcinol, arbutin, and glycolic acid were preincubated with transfectant HaCaT cells for 3 h and then ultraviolet B (UVB) was irradiated. $\textrm{NF}_{-{\kappa}}\textrm{B}$ activation was measured with the SEAP reporter gene assay using a fluorescence detection method. Of the Mis tested, kojic acid ($IC_{50}$/ = 60 $\mu$M) was found to be the most potent inhibitor of UVB-upregulating $\textrm{NF}_{-{\kappa}}\textrm{B}$ activation in transfectant HaCaT cells, which is followed by niacinamide ($IC_{50}$/= 540 $\mu$M). Pretreatment of the transfectant HaCaT cells with the Mis, especially kojic acid and niacinamide, effectively lowered $\textrm{NF}_{-{\kappa}}\textrm{B}$ binding measured by electrophoretic mobility shift assay. Furthermore, these two inhibitors remarkably reduced the secretion level of IL-6, one of melanotrophic factors, triggered by UV-radiation of the HaCaT cells. These observations suggest that Mis working at the in vivo level might act partially through the modulation of the synthesis of melanotrophic factors in keratinocyte.

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The Influence of Skin surface temperature on Transdermal Absorption of Active Substances and Changes in Skin Condition (피부표면온도가 유효물질 경피 흡수 및 피부 상태변화에 미치는 영향)

  • Kwon, Hye-Jin;Han, Sa-Ra;Jung, Na-Seul
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.1
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    • pp.299-304
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    • 2019
  • This study was conducted to investigate the effects of skin surface temperature on transdermal absorption of active substances and changes in skin condition. A transdermal absorption test was conducted using an emulsion containing 10% heat-resistant niacinamide, and the same emulsion was used in a clinical trial to test the thermal effects. As a result, absorption was found to be 2 times greater at 10 minutes and 3 times greater at 15 minutes after application at $42^{\circ}C$ than that of compared to normal skin temperature. During the clinical evaluation on skin, no clinical subjects showed any specific adverse reactions, while moisture and oiliness showed statistically significant effects at higher temperatures. Based on these results, it was confirmed that thermal warmer improves the absorption of active substances and had a positive effect on skin condition. It is deemed that this study will provide a preliminary data to support the development of various beauty devices using heat.

Comparision of Preparation Methods for Water Soluble Vitamin Analysis in Foods by Reversed-Phase High Performance Liquid Chromatography (역상 고속 액체 크로마토그래피에 의한 식품 중 수용성 비타민 분석을 위한 전처리법의 비교)

  • Kim, Hyung-Soo;Jang, Duck-Kyu;Woo, Dong-Kyun;Woo, Kang-Lyung
    • Korean Journal of Food Science and Technology
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    • v.34 no.2
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    • pp.141-150
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    • 2002
  • Owing to a need for simple extraction and purification for analysis of water soluble vitamins in food samples by RP-HPLC with UV-detector, the methods of bromelain and protease hydrolysis and $C_{18}$ Sep-Pak solid phase extraction were employed. The recoveries of standard water soluble vitamins by the bromelain and protease hydrolysis and $C_{18}$ Sep-Pak solid phase extraction were significantly high compared to AOAC methods in most of vitamins. The contents of pyridoxal determined with protest in the pork was similar, but in the bromelain hydrolysis and AOAC method, was high compared to the results of reference. The niacinamide, thiamin and riboflavin determined with bromelain and protease hydrolysis showed similar values to the results of references. In the potato, pyridoxamine was detected in the AOAC method, which was not detected in the bromelain and protease hydrolysis methods. Pyridoxal contents in the protease hydrolysis and AOAC methods were very similar to the results of references. The recoveries of fortified standard vitamins in food samples were significantly high and accurate compared to those of AOAC methods. The extraction and purification with $C_{18}$ Sep-Pak solid extractor might be considered superior method for the determination of water soluble vitamins in food samples.

Studies on Application of Sterilization Method Using Ultra Violet Radiation for Liquid Preparations (자외선살균법(紫外線殺菌法)의 액제(液劑)에 대(對)한 응용(應用)의 검토(檢討))

  • Kim, Yong-Bae;Lee, Sang-Hee;Kim, Geon-Chee;Kang, Gun-Il
    • Journal of Pharmaceutical Investigation
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    • v.2 no.1
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    • pp.58-62
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    • 1972
  • The stabillities of taurine, niacinamide, and pyridoxine hydrochloride under exposure to ultra violet radiation in liquid preparations were studied. And sterilization effects for E. coli in both water and liquid preparations were also comparatively evaluated. The above mentioned organic compounds were stable under this experimental conditions and viable count of E. coli reveals that organic compounds dissolved in solution display protective action for microorganisms under UV-irradiation.

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Skin permeability of compounds loaded within dissolving microneedles dependent on composition of sodium hyaluronate and carboxymethyl cellulose

  • Park, Youbin;Kim, Bumsang
    • The Korean Journal of Chemical Engineering
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    • v.34 no.1
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    • pp.133-138
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    • 2017
  • Dissolving microneedles are transdermal delivery systems designed to mechanically penetrate the skin and fully dissolve in the skin in a minimally invasive manner. In this study, the skin permeability of compounds encapsulated in microneedles was controlled by changing the composition of microneedle materials. Sodium hyaluronate (SH) and carboxymethyl cellulose (CMC) were chosen as structural materials and amylopectin was used to increase the mechanical strength of microneedles. To determine the effect of microneedle composition on skin permeability, microneedle properties such as mechanical strength and solubility were investigated according to various compositions of SH and CMC. When the CMC fraction in the needle increased, the mechanical strength of the microneedle increased, leading to high skin permeability of rhodamine B, a model compound. Using microneedles, significantly higher skin permeability of niacinamide was also obtained. These results indicate that the microneedles developed in this study improved the skin permeability of compounds loaded in the needle, and the skin permeability could be tuned by changing the composition of microneedle materials.

A Study on Synergistic Antibacterial Activity of Cosmetic Ingredients (화장품 성분들의 세균에 대한 항균 시너지 효과 연구)

  • Kim, So-Hee;Yun, Yuna;An, Susun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.44 no.1
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    • pp.1-7
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    • 2018
  • In this study, 1,2-hexanediol, ethylhexylglycerin, and glyceryl caprylate known as skin conditioning agents with antimicrobial activity in cosmetics were investigated for their antimicrobial activity against gram-positive and gram-negative bacteria when combining them with various ingredients in cosmetic formulations. Seven kinds of substances expected to improve the antibacterial activity in formulations were selected to blend with these three compounds.; 1,3-propanediol, 1,3-butanediol, glycerin, dipropylene glycol, niacinamide, EDTA-2Na, and ethanol. The checkerboard assay was conducted to confirm the antibacterial synergy of these seven substances and the three skin conditioning agents. Consequently, ethanol and EDTA-2Na were detected as the significant materials with synergistic effect as well as ethanol showed antibacterial synergy with all the three compounds. When seven of selected materials were mixed with glyceryl caprylate, there were synergistic or additive activity against S. aureus, gram-positive bacteria and it was suggested that the combination with 1,2-hexanediol and glyceryl caprylate was useful to control both gram-positive and gram-negative bacteria when there were four kinds of polyols. This study is meaningful in that it confirmed the antibacterial synergy of the skin conditioning agents with antimicrobial activity and the main raw materials frequently used in cosmetics, thereby contributing to prediction of the antibacterial activity of the skin conditioning agents in cosmetic formulations.