• Title, Summary, Keyword: nude mice

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Hair Growth Promoting Effect of Radish Crude Saponin Extract on Athymic Nude Mice

  • Kim, Hyun-Kyoung
    • International journal of advanced smart convergence
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    • v.8 no.1
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    • pp.184-195
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    • 2019
  • This study investigates the hair restoration efficacy of selected radish saponin extracts on nude mice. Nude mice genetically predisposed to pattern balding were used in this study. Our study revealed the underlying mechanism of stimulating hair growth in athymic nude mice by repair the nu/nu follicular keratin differentiation defect. Thus, the topical application of radish saponin may represent a novel strategy for the management and therapy of certain forms of alopecia. The term of hair density of PEE treated nude mice were significantly increase as compared with of control nude mice. Histological observation of skin sample showed no hair follicle or only distorted hair follicles were observed in the control samples, in contrast, by the PEE treatment groups showed a fully formed and increased the number of hair follicles up to three times higher than that of control group in terms of the number of hair follicles in nude mouse skin.PEE treated mice the number of BrdU-labeled keratinocytes per anagen follicle increased significantly, especially in the follicular bulbs and outer root sheath compared with the control mice. Moreover, PEE-treated nude mice also exhibited a significant increase in the number of BrdU-labeled epidermal keratinocyte proliferation.

Attempts to Transfer Immunity against Clonorchis in Nude and DS Mice (근교계 nude 및 DS 마우스 폐강삼출세포와 혈청의 간흡충 감염에 대한 면역이입의 시도)

  • Choe, Dong-Ik;Jeong, Dong-Il;Gang, Deok-Hui
    • The Korean Journal of Parasitology
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    • v.29 no.4
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    • pp.371-380
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    • 1991
  • The effects of peritoneal exudate cells(PEC) and sera of athymic nude and DS mice infected with Clonorchis sinensis metacercariae or sensitized by injection of metabolic products into footpad on transfer of immunity against the fluke to the syngeneic mice were studied. There was no significant difference in eggs per gram pattern between the sensitized and control groups, and between nude and DS mice. However, the worm burdens were slightly greater in nude mice than in DS mice. Also, a few plaque forming cells were found in only DS mice given PEC and serum from Group II DS mice. In the light of these results, it is likely that PEC and sera of nude or DS mice which are deficient, at least partially, in the cellular immune system are unable to transfer immunity against C. sinensis to syngeneic recipients.

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MAKING IN VIVO MODEL TO STUDY ABOUT HUMAN ORAL CANCER (I) (구강암 연구를 위한 동물실험모델의 개발(I))

  • Park, Hyung-Kook;Kim, Yong-Kack
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.19 no.3
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    • pp.300-310
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    • 1997
  • In order to make in vivo model of human oral squamous cell cancer, we brought up BALB/C nude mice in specially designed housystem, and maintained some kind of human oral squamous cancer cell lines ; KB, SCC-4, SCC-9, SCC-15, SCC-25. Various concentration of cancer cells were inoculated subcutaneouly into flank area of nude mice. We observed each nude mouse more than 5 weeks after tumor inoculation. We appraised the results, measured the tumor size, and calculated the growing tumor volumes after tumor inoculation according to cancer cell line and concentration of cancer cells in media. Some cancer cell lines were rapidly growing in nude mice, but some cancer cell line couldn't grow in nude mice and resorbed completely. And in some cancer cell line, some nude mice showed continuously growing tumor, but other didn't show any tumor growing. And as a new try, we implanted specially disigned caps on the back of nude mice, and cancer cell lines were brought into the caps with media. We removed the cap after 1 week, and observed over 4 weeks. The shape and size of growing tumor were observed.

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Evaluation of the Potential Risk of Porcine Endogenous Retrovirus (PERV) Infection in Nude Mice

  • Bae, Eun-Hye;Jung, Yong-Tae
    • Journal of Microbiology and Biotechnology
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    • v.21 no.4
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    • pp.387-390
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    • 2011
  • Nude mice (BALB/c) were grafted with human 293 cells and PERV (porcine endogenous retrovirus)-IRES-EGFP (a packageable retroviral vector plasmid containing an internal ribosome entry site-enhanced green fluorescent protein)-producing pig PK15 cells in order to determine whether the pig cells could transmit PERV-IRES-EGFP to mice and human 293 cells in vivo. None of the transplanted human 293 cell lines were infected by PERV, but PCR analysis identified PERV-B provirus integration into both the heart and salivary gland of the inoculated nude mice. Our data indicate that hearts and salivary glands can be used to identify PERV-B receptors.

Histologic study of bone-forming capacity on polydeoxyribonucleotide combined with demineralized dentin matrix

  • Kim, Seok-Kon;Huh, Chang-Kyu;Lee, Jae-Hoon;Kim, Kyung-Wook;Kim, Moon-Young
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.38
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    • pp.7.1-7.5
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    • 2016
  • Background: This study examined the osteoinductive activity of demineralized dentin matrix (DDM) from human and polydeoxyribonucleotide (PDRN) for nude mice. Methods: Twenty healthy nude mice, weighing about 15~20 g, were used for the study. DDM from human and PDRN were prepared and implanted subcutaneously into the dorsal portion of the nude mice. The nude mice were sacrificed at 1, 2, and 4 weeks after grafting and evaluated histologically by hematoxylin-eosin and Masson's trichrome staining. The specimens were also evaluated via a histomorphometric study. Results: The DDM and PDRN induced new bone, osteoblasts, and fibroblasts in soft tissues. The histological findings showed bone-forming cells like osteoblasts and fibroblasts at 1, 2, and 4 weeks. New bone formation was observed in the histomorphometric study. In particular, the ratio of new bone formation was the highest at 2 weeks compared with the first week and fourth week. Conclusions: In this study, we showed that the PDRN used in this experimental model was able to induce bone regeneration when combined to the DDM.

Ameliorate Effect of Black Ginseng on HepG2 Cell transplanted in BALB/c Nude Mice (HepG2 세포주를 이식한 BALB/c Nude Mice에서 흑삼의 고형암 개선효과)

  • Kang, Shin-Jyung;Han, Jung-Soon;Kim, Ae-Jung
    • The Korean Journal of Food And Nutrition
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    • v.28 no.2
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    • pp.241-246
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    • 2015
  • The aim of this study was to examine the ameliorating effect of black ginseng on the growth of the HepG2 cell transplanted tumor in BALB/c nude mice. 27 male BALB/c nude mice (all six weeks old) were randomly divided into three groups: the control group, the first treatment group (HepG2300RG, using 300 mg/kg red ginseng), and the second treatment group (HepG2300BG, using 300 mg/kg black ginseng). The HepG2300BG in the HePG2 cells showed increased mean survival time than that of red ginseng group. The size and volume of the tumor in the 300BG group showed significant reduction compared to those of the HepG2300RG group (p<0.05). The body weight and liver weight of the HepG2300RG group was not significantly different with control and HepG2300BG group. The serum levels of ALT and AST in the HepG2300RG and HepG2300BG group were significantly lower than those of the control group. In conclusion, these results suggest that the black ginseng may have possible anti-tumor activities.

Tumor-Suppressing Effects of Saponin-SOD in Nude Mice (Saponin-SOD가 SNUC-4 Cell에 의해 유발편 대장암 Nude Mice의 암 성장 억제 효과)

  • 박수진;강명화
    • Journal of the East Asian Society of Dietary Life
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    • v.14 no.4
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    • pp.338-342
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    • 2004
  • This study was performed to measure the antioxidant effects of red ginseng extracts which antioxidation had been promoted through enzyme hydrolysis. In order to observe their tumor-suppressing effects, an anti-cancer medicine and Saponin-SOD, which was a highly antioxidant beverage made from red ginseng saponin adding SOD-like rice (with embryo buds) extracts, were administered to nude mice with large intestine cancer induced. There was a significant increase in the content of phenolic compounds as the enzyme was added. The red ginseng extracts showed a high electron-donating ability with the passage of time. The electron-donating ability was particularly high in the enzyme-treated red ginseng extract, and also observed as high in Saponin-SOD. The lipid-peroxide generation was inhibited depending on the concentration of Saponin-SOD added; the addition of 0.625% Saponin-SOD served to decrease the inhibition level up to 65% compared with the case of no addition (100%). As a result, it could be assumed that Saponin-SOD would strongly inhibit the oxidation of ghost membrane. After the cancer was induced in nude mice through the injection of SNUC-4 cell, there was a significant inhibition in the growth of tumors in nude mice into which Saponin-SOD were injected; the growth of tumors was gradually decreasing with the passage of time after the cancer induction. In particular, when Saponin-SOD was administered together with an anti-cancer medicine, the synergic effect was observed. In conclusion, Saponin-SOD, when used with an anti-cancer medicine, is expected to reduce the amount of free radical and lipid peroxide, which are known to cause harmful effects occurring from the internal application of medicine.

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Establishment of a Pancreatic Cancer Stem Cell Model Using the SW1990 Human Pancreatic Cancer Cell Line in Nude Mice

  • Pan, Yan;Gao, Song;Hua, Yong-Qiang;Liu, Lu-Ming
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.2
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    • pp.437-442
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    • 2015
  • Aim: To establish a pancreatic cancer stem cell model using human pancreatic cancer cells in nude mice to provide a platform for pancreatic cancer stem cell research. Materials and Methods: To establish pancreatic cancer xenografts using human pancreatic cancer cell line SW1990, nude mice were randomly divided into control and gemcitabine groups. When the tumor grew to a volume of $125mm^3$, they treated with gemcitabine at a dose of 50mg/kg by intraperitoneal injection of 0.2ml in the gemcitabine group, while the mice in control group were treated with the same volume of normal saline. Gemcitabine was given 2 times a week for 3 times. When the model was established, the proliferation of pancreatic cancer stem cells was observed by clone formation assay, and the protein and/or mRNA expression of pancreatic stem cell surface markers including CD24, CD44, CD133, ALDH, transcription factors containing Oct-4, Sox-2, Nanog and Gli, the key nuclear transcription factor in Sonic Hedgehog signaling pathway was detected by Western blot and/or RT-PCR to verify the reliability of this model. Results: This model is feasible and safe. During the establishment, no mice died and the weight of nude mice maintained above 16.5g. The clone forming ability in gemcitabine group was stronger than that of the control group (p<0.01). In gemcitabine group, the protein expression of pancreatic cancer stem cell surface markers including CD44, and ALDH was up-regulated, the protein and mRNA expression of nuclear transcription factor including Oct-4, Sox-2 and Nanog was also significantly increased (P<0.01). In addition, the protein expression of key nuclear transcription factor in Sonic Hedgehog signaling pathway, Gli-1, was significantly enhanced (p<0.01). Conclusions: The pancreatic cancer stem cell model was successfully established using human pancreatic cancer cell line SW1990 in nude mice. Gemcitabine could enrich pancreatic cancer stem cells, simultaneously accompanied by the activation of Sonic Hedgehog signaling pathway.

Protection Mechanism of Infection Against Tyzzer's Disease (마우스에 있어서의 Tyzzer병(病) 감염방어기구(感染防禦機構))

  • Lee, Yong Soon;Lee, Jang Nak
    • Korean Journal of Veterinary Research
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    • v.20 no.1
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    • pp.33-38
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    • 1980
  • 1. In adult ICR-SLC mice, significant increase in the occurrence of the Tyzzer's disease was noted when the reticuloendothelial system was blockaded with the carbon particles. The most suitable bloakading time was 2 hours before inoculating Tyzzer's organisms, the route was intraperitoneal and the dose was 3mg/body. 2. Survival rate of the experimental group ICR-SLC suckling mice receiving BCG ($3{\times}10^8$) 6 days prior to intraperitoneal inoculation of Tyzzer's organisms was 80 percent, but the control group was 17%. 3. In comparing the survival rate of the nude/nude mice with the nude/+ mice, the former congenitally thymus deficient mice are 11 percent, but the latter are 67 percent.

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Bone Induction by Demineralized Dentin Matrix in Nude Mouse Muscles

  • Kim, Kyung-Wook
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.36 no.2
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    • pp.50-56
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    • 2014
  • Purpose: This study examined the osteoinductive activity of demineralized human dentin matrix for nude mice. Methods: Twenty healthy nude mice weighing about 15 to 20 g were used for study. Demineralized human dentin matrix was prepared and implanted into the dorsal portion of nude mice (subcutaneous), which were sacrificed at two, four, and eight weeks after demineralized dentin matrix grafting and evaluated histologically by H&E and Masson trichrome staining. The specimens were also evaluated histomorphometrically. Results: The demineralized dentin matrix induced bone and cartilage formation independently in soft tissues. Histological examination showed bone-forming cells such as osteoblasts and fibroblasts at two, four, and eight weeks. Conclusion: These results suggest that demineralized human dentin matrix has osteoinductive ability, and is a good alternative to autogenous bone graft materials.