• Title, Summary, Keyword: olive flounder

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Generation of polyclonal antiserum to olive flounder (Paralichthys olivaceus) immunoglobulin by immunization of rabbit with plasmids containing heavy chain gene of olive flounder immunoglobulin

  • Kim, Gi-Hong;Kwon, Se-Ryun;Kim, Chun-Soo;Lee, Eun-Hye
    • Journal of fish pathology
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    • v.19 no.2
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    • pp.183-188
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    • 2006
  • In fish vaccinology, the secondary antibodies against fish immunoglobulins (Igs) are necessary to measure specific humoral immune responses in immunized fish. In the present study, polyclonal antiserum against olive flounder (Paralichthys olivaceus) IgM heavy chain was generated by intramuscular immunization of rabbit with Escherichia coli/eukaryotic shuttle vector containing open reading frame (ORF) of olive flounder IgM heavy chain. Western blot analysis demonstrated the specific activity of the rabbit antiserum with reduced olive flounder serum H chain at dilutions up to 1:1000. Titer of immunized rabbit serum against olive flounder serum was significantly higher than that of pre-immunized rabbit serum when determined by ELISA.

Cloning and expression of cDNA for chemokine receptor 9 from Olive flounder, Paralichthys olivaceus

  • Kim, Mu-Chan;An, Geun-Hee;Park, Chan-Il
    • Journal of fish pathology
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    • v.20 no.3
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    • pp.299-306
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    • 2007
  • Cysteine-cysteine chemokine receptor 9 (CCR9) homologue cDNA was isolated from olive flounder leukocyte cDNA library. Olive flounder CCR9 homologue consisted of 1709 bp encoding 367amino acid residues. When compared with other known CCR peptide sequences, the most conserved region of the olive flounder CCR9 peptide is the seven transmembranes. A phylogenetic analysis based on the deduced amino acid sequence showed the homologous relationship between the olive flounder CCR9 sequence and that of Mouse CCR9. The olive flounder CCR9 gene was predominantly expressed in the Peripheral blood leukocytes (PBLs), kidney, spleen, and gills.

Cloning and Characterization of DAP10 homologue gene from Olive Flounder, Paralichthys olivaceus

  • Park, Chan-Il;Kim, Mu-Chan;Hwang, Jee-Youn;Kim, Ki-Hyuk;Kim, Joo-Won
    • Journal of fish pathology
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    • v.19 no.3
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    • pp.227-233
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    • 2006
  • Olive flounder immunoreceptor DAP10 homologue cDNA was cloned from a peripheral blood lymphocytes (PBLs) cDNA library. The length of the olive flounder DAP10 cDNA is 473bp and it contains an open reading frame of 234bp. The predicted polypeptide sequence is 78 amino acids, consisting of a 22-amino acid leader, an 11-amino acid extracellular domain, a 21-amino acid transmembrane segment, and a 24-amino acid cytoplasmic domain. The amino acid sequence of olive flounder DAP10 has 56%, 50%, 32%, 31%, and 31% sequence identity with zebrafish DAP10, catfish DAP10, cattle DAP10, rat DAP10 and Monkey DAP10, respectively. Olive flounder DAP10 has a conserved aspartic acid in the transmembrane domain and a phophatidylinositol-3 kinase-binding site (YxxM/V) in the cytoplasmic region. Genomic organization reveals that olive flounder DAP10 comprises five exons and four introns. A phylogenetic analysis based on the deduced amino acid sequence grouped the olive flounder DAP10 with other species DAP10. In RT-PCR analysis, DAP10 transcripts were detected predominantly in PBLs, kidney, spleen and intestine.

Preparation and Texture Characterization of Surimi Gel Using a Unmarketable Rearing Olive Flounder (저상품성 양식 넙치를 이용한 연제품 제조 및 텍스튜어 특성)

  • Cha, Seon-Heui;Jo, Mi-Ran;Lee, Jung-Suck;Lee, Ji-Hyeok;Ko, Joo-Young;Jeon, You-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.2
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    • pp.109-115
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    • 2009
  • The properties of surimi gel from Olive flounder Paralichthys olivaceus were evaluated with addition of various food additives and by heating under different conditions. The optimum heating conditions for get good textured surimi gel from Olive flounder was 40 minutes at $85^{\circ}C$. Optimum texture characteristics such as hardness, cohesiveness, chewiness, gumminess and gel strength of olive flounder fish paste can be acquired by washing the paste 2 times and by adding sodium chloride (2.5%), sodium polyphosphate (0.3%) and starch (4%), respectively. Furthermore, we compared gel texture characteristics and strength of manufactured Olive flounder fish surimi with commercial Alaska pollack to determine their commercial applicability. Texture characteristics and gel strength of Olive flounder surimi were higher than those of the commercial Alaska pollack surimi. In addition, both lightness and whiteness were higher in surimi gel from Olive flounder than from the commercial Alaska pollack.

EST-based Identification of Genes Expressed in the Brain of the Olive Flounder Paralichthys olivaceus

  • Lee, Jeong-Ho;Noh, Jae-Koo;Kim, Hyun-Chul;Park, Choul-Ji;Min, Byung-Hwa;Kim, Young-Ok;Kim, Jong-Hyun;Kim, Kyung-Kil;Kim, Woo-Jin;Myeong, Jeong-In
    • Fisheries and aquatic sciences
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    • v.12 no.4
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    • pp.286-292
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    • 2009
  • We have constructed a cDNA library using brain samples of olive flounder Paralichthys olivaceus. Here, we described the study on gene identification by screening 356 clones from the brain cDNA library of olive flounder. Here, we screened 356 clones from the library to identify genes. Of these, 176 (49.5%) were identified as orthologs of known genes from olive flounder and other organisms. Among the 176 EST clones, 33 (18.7%) represented 11 unique genes that are identical to expressed sequence tags (ESTs) reported for olive flounder, and 120 (68.2%) represented 102 unique genes known from other organisms. The percentage of unknown genes (50.5%) is higher than in other olive flounder cDNA libraries (Lee et al., 2003, 2006, 2007), reflecting the high complexity of brain tissue. Further studies of expression characterization and developmental behavior related to these genes should provide useful insight into the physiological functions of the brain in olive flounder.

Cloning and Characterization of Two Distinct CD3 Genes from Olive Flounder Paralichthys olivaceus

  • Kim, Mu-Chan;Park, Chan-Il
    • Fisheries and aquatic sciences
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    • v.8 no.2
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    • pp.56-64
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    • 2005
  • Two distinct CD3 homologue genes, $CD3\gamma/\delta\;and\;CD\varepsilon$, were isolated from a olive flounder leukocyte cDNA library and a BAC library. $CD3\gamma/\delta$ consisted of 961 bp encoding 178 amino acid residues, and $CD3\varepsilon$ consisted of 1006 bp encoding 164 amino acid residues. When compared with other known CD3 peptide sequences, the most conserved region of the two olive flounder CD3 chain peptides are the cytoplasmic domain and the least conserved are the extracellular domain. A phylogenetic analysis based on the deduced amino acid sequence grouped the two olive flounder CD3 sequences with $CD3\varepsilon$ and $CD3\gamma/\delta$, respectively. The olive flounder CD3 cluster (consisting of $CD3\varepsilon\;and\;CD3\gamma/\delta$) spans only 10.4 kb. The $CD3\varepsilon\;and\;CD3\gamma/\delta$ genes are oppositely transcribed only 3.8 kb apart. Both olive flounder CD3 genes have five exons. The two olive flounder CD3 genes were predominantly expressed in PBLs, kidney, spleen, and gills.

Sustainability of Olive Flounder Production by the Systems Ecology -II. Simulating the Future of Olive Flounder Aquaculture on the Land- (시스템 생태학적 접근법에 의한 넙치생산의 지속성 평가 -2. 넙치 육상양식산업에 대한 예측-)

  • Kim Nam Kook;Son Ji Ho;Kim Jin Lee;Cho Eun Il;Lee Suk Mo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.6
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    • pp.660-665
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    • 2002
  • In Korea, an olive flounder is very popular fish food item. However, due to the increasing human population, the present catches of the olive flounder may not be sufficient to satisfy the present demand. To increase the supply of the olive flounder, aquaculture has been begun. An interest in the aquaculture of the olive flounder has been increased recently because of its characteristics of good growth and high price in the market, However, the productivity of the olive flounder aquaculture depends on economic inputs such as fuels, facilities, and labor. The rapid growths of the olive flounder aquaculture and the concerns about economic and ecological sustainability have focused peoples attention on the aquaculture industry. In this study, an energy systems model was built to simulate the variation of sustainability on the aquaculture of olive flounder, The results of simulation based on calibration data in 1995 show that olive flounder production yield and asset slowly increase to steady state because of the law of supply and demand. The results of simulation based on the variation of oil price show that the more increase the oil price, the more decrease the olive flounder economic yield and asset. Energy sources required for systems determine the sustainability of systems. Conclusionally, the present systems of the olive flounder aquaculture should be transformed to ecological-recycling systems or ecological engineering systems which depend on renewable resources rather than aquaculture systems which depend on fossil fuels, and be harmonized with the fishing fisheries by the sustainable use of renewable resources in the carrying capacity.

Cloning of Melanin Concentrating Hormone cDNA Gene from Olive Flounder (Paralichthys olivaceus) (넙치 (Paralichthys olivaceus)에서 멜라닌 농축 호르몬 cDHA 유전자의 클로닝)

  • JEON Jeong Min;SONG Young Hwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.5
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    • pp.442-448
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    • 2003
  • Melanin concentrating hormone (MCH) regulating color change of fish skin was identified from brain cDNA library of Olive flounder (Paralichthys olivaceus) during the analysis of Expressed Sequence Tags (ESTs). Olive flounder MCH gene consisted of 598 nucleotides encoding 150 amino acids. Olive flounder MCH protein revealed to contain signal peptide of 19 amino acid residues, pro-MCH of 131 amino acids being processed to biologically active and mature form of hormone with 25 amino acid residues at the carboxyl terminus. A comparative structural analysis revealed that Olive flounder MCH precursor had low sequence identity with other fish species and mammalian counterparts, while the amino acid sequences of mature hormone had a relatively high identity and more conserved. RT-PCR analysis revealed that olive flounder MCH precersor gene was expressed spectically only in the brain and not in other tissues.

Development of Allotriploid Embryos from Female Olive Flounder Paralichthys olivaceus Crossed with Male Starry Flounder Platichthys stellatus (넙치(Paralichthys olivaceus)와 강도다리(Platichthys stellatus)간 유도된 잡종 3배체의 난발생)

  • Jung, Hyo Sun;Ko, Min Gyun;Lee, Hyo Bin;Kim, Dong Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.5
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    • pp.628-634
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    • 2016
  • We investigated the characteristics and rate of development of allotriploid embryos derived from a cross between female olive flounder Paralichthys olivaceus and male starry flounder Platichthys stellatus. The allotriploidy was induced by cold shocking fertilized eggs three minutes post-fertilization at 3°C for 45 minutes. The average cellular DNA content of the allotriploid embryos was 2.06±0.03 pg/cell, which is equal to the sum of the cellular DNA content of a diploid olive flounder (1.42 pg/cell) and a haploid starry flounder (0.66 pg/haploid cell). The first cleavage, midblastula, gastrula and Kupffer's vesicle appearance stages of the allotriploid eggs began at 1.5, 8, 13 and 26 hours after cold shocking at 18°C, respectively. The developmental rate of allotriploid eggs was equivalent to that of diploid and triploid olive flounder eggs at 10, 14 and 18°C. However, the hatching times of allotriploid eggs, 7 h at 10°C, 5 h at 14°C and 4 h at 18°C, were earlier than those of diploid and triploid olive flounder.

Comparison of RAPD Profiles and Phenotypical Characters of Streptococcal Strains (연쇄상구균의 표현형적 특성과 RAPD profiles 비교)

  • Song, Jin-Gyeong;Kim, Jong-Hun;Kim, Eun-Hui
    • Journal of fish pathology
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    • v.16 no.1
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    • pp.51-59
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    • 2003
  • Streptococcal infection is one of the most serious disease of cultured olive flounder, Paralychthys olivaceus in Korea and caused by more than one species. However, there has been considerable confusions about the taxonomic position of the fish pathogenic streptococci. In this study, We performed the randomly amplified polymorphic DNA(RAPD) pattern analysis to evaluate the possible classification in 8 streptococci isolated from diseased olive flounder and reference strains based on their DNA structure. RAPD PCR with DNA solution prepared by simple boiling and 10-mer random primer was appeared to be a good tool for discrimination of different streptococcal strains. Phenotypical characters by simple biological test and API 20 Strep corresponded well to the specific profiles of RAPD in streptococcal isolates of this study. Therefore, the RAPD profile was considered as one of differential characters to discriminate the streptococcal isolates from diseased olive flounder.