• Title, Summary, Keyword: ovariectomized mice

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Effects of Herbal-Acupuncture with Evodiae Fructus at KI10 on Osteoporosis in Ovariectomized Mice (음곡(陰谷) 오수유(吳茱萸) 약침(藥鍼)이 난소적출(卵巢摘出)생쥐의 골다공증(骨多孔症)에 미치는 영향(影響))

  • Choi, Sung-Hoon;Lee, Byung-Ryul;Yang, Gi-Young;Kim, Jae-Kue;Seo, Young-Suk;Yim, Yun-Kyoung
    • Korean Journal of Acupuncture
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    • v.27 no.2
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    • pp.217-242
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    • 2010
  • Objective : This study is aimed to evaluate the effects of Evodiae Fructus herbal-acupuncture (EF-HA) at KI10 on osteoporosis induced by ovariectomy in mice. Method : Mice underwent bilateral ovariectomy. After recovering, the ovariectomized (OVX) mice were treated by needle prick, saline injection, herbal acupuncture with Evodiae Fructus (EF-HA) at KI10 for 8 weeks. Result : 1. EF-HA at KI10 significantly inhibited the overgrowth of tibia in ovariectomized mice. 2. NP at KI10 significantly restored the tibial BMD (bone mineral density) in ovariectomized mice. 3. EF-HA at KI10 significantly restored the phosphorus and creatinine levels in ovariectomized mice serum. 4. EF-HA at KI10 significantly restored the tibial Ca and P levels in ovariectomized mice. 5. EF-HA at KI10 significantly reduced the tibial osteoclast-like cells in ovariectomized mice. 6. EF-HA at KI10 significantly inhibited the overgrowth of tibial GPL (growth plate length) in ovariectomized mice. Conclusion : EF-HA at KI10 has protective and therapeutic effect for osteoporosis in ovariectomized mice. Thus, it is suggested that EF-HA can be an useful therapeutics in clinical field after further researches.

Effects of the Herbal-Acupuncture with Corni Fructus Extract at Eumgok(KI10) on Osteoporosis in Ovariectomized Mice (음곡(陰谷)(KI10) 산수유 약침이 난소적출 생쥐의 골다공증에 미치는 영향)

  • Kim, Kwang-Sung;Lee, Byung-Ryul;Yang, Gi-Young;An, Taek-Won;Yim, Yun-Kyoung
    • Korean Journal of Acupuncture
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    • v.27 no.1
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    • pp.63-85
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    • 2010
  • Objective & Method: The purpose of this study is to investigate the effects of herbal-acupuncture with Corni Fructus extract (CF-HA) at Eumgok(KI10) on osteoporosis in ovariectomized (OVX) ddy mice. I carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, the levels of serum ALP, osteocalcin, Ca, P and the levels of tibial Ca, P, Ca/P ratio, and we performed histological and histomorphological analysis as well. Result: 1. CF-HA at Eumgok(KI10) significantly did not increase the level of bone mineral density in overiectomized mice. 2. CF-HA at Eumgok(KI10) significantly decreased the level of serum phosphorus in ovariectomized mice and sinificantly increased the level of serum calcium in ovariectomized mice. 3. CF-HA at Eumgok(KI10) significantly increased the levels of tibial calcium and phosphorus in ovariectomized mice. 4. CF-HA at Eumgok(KI10) significantly decreased the number of tibial osteoclast like cell in ovariectomized mice. 5. In the histomorphometric analysis of tibia, GPL(growth plate length) was significantly decreased by CF-HA at Eumgok(KI10) in ovariectomized mice. Conclusions: These results suggest that CF-HA at Eumgok(KI10) may have useful therapy effects on osteoporosis in ovariectomized mice.

Effects of Adenophora triphylla var. japonica Extract on Cognitive Function in Ovariectomized Mice (난소절제 마우스에서 잔대 추출물의 인지능 개선 효과)

  • Jang, Hwan Hee;Kim, Haeng Ran;Lee, Young Min
    • Journal of the East Asian Society of Dietary Life
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    • v.27 no.4
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    • pp.442-449
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    • 2017
  • Adenophora triphylla var. japonica extract has been reported to have estrogenic activity and beneficial effects in postmenopausal women requiring estrogen replacement therapy. This study investigated the effects of A. triphylla extract on cognitive function in ovariectomized mice. Water extracts from A. triphylla were administrated to ovariectomized mice fed a high fat diet for 6 weeks. The step through latency time in the passive avoidance test was shortened in ovariectomized mice as compared with sham-operated mice. However, administration of A. triphylla extracts had a tendency to prolong the latency time in ovariectomized mice. Moreover, A. triphylla supplementation caused a significant decrease in mean escape latency in the water maze test. These results indicate that A. triphylla could be used to improve estrogen deficiency-related learning and memory impairments in postmenopausal women.

Effects of Herbal-acupuncture with $Artemisiae$ $Vulgaris$ $Folium$ Extract at $KI_{10}$($Eumgok$) on Osteoporosis in Ovariectomized Mice (음곡 애엽약침이 난소적출 흰쥐의 골다공증에 미치는 영향)

  • Jung, Ju-Youl;Kim, Jung-Ho;Kim, Young-Il
    • Journal of Acupuncture Research
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    • v.28 no.6
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    • pp.117-137
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    • 2011
  • Objectives & Methods : The purpose of this study is to observe the effects of herbal-acupuncture with $Artemisiae$ $Vulgaris$ $Folium$ extract(AaH-HA) at $KI_{10}$($Eumgok$) on osteoporosis in ovariectomized(OVX) ddy mice. We carried out several experimental items to analyze the changes in body weight, urine, weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results : 1. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the reduction phosphorus level in serum in ovariectomized mice. 2. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the reduction creatinine level in serum in ovariectomized mice. 3. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the increase of tibial osteoclast cells in ovariectomized mice. 4. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the reduction of TBV(trabecular bone volume) and TBT(trabecular bone thickness) in ovariectomized mice. 5. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the overgrowth of tibial growth plate length(GPL) in ovariectomized mice. Conclusions : In conclusion, our study suggested that Herbal-acupuncturing with AaH-HA at $KI_{10}$ can be effective treatment for osteoporosis.

Effects of Herbal-Acupuncture with Rosae Laevigatae Fructus Extract at KI10(Umgok) on Osteoporosis in Ovariectomized Mice (음곡(KI10) 금앵자 약침이 난소적출 생쥐의 골다공증에 미치는 영향)

  • Kim, Dong-Soo;Kim, Jeoung-Ho;Kim, Young-Il
    • Journal of Pharmacopuncture
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    • v.12 no.4
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    • pp.51-62
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    • 2009
  • Objective & Methods : The purpose of this study was to observe the effects of herbal-acupuncture with Rosae Laevigatae Fructus extact(RLF-HA) at KI10(Umgok) on osteoporosis in ovariectomized( OVX) ddy mice. We carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results : 1. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) and saline injection at KI10(Umgok) significantly inhibited the reduction of tibial calcium level in ovariectomized mice. 2. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) significantly inhibited the increase of tibial osteoclast cells in ovariectomized mice. 3. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) significantly inhibited the reduction of tibial trabecular bone thickness(TBT) in ovariectomized mice. 4. Herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) significantly inhibited the overgrowth of tibial growth plate length(GPL) in ovariectomized mice. Conclusion : These results suggested that herbal-acupuncture with Rosae Laevigatae Fructus at KI10(Umgok) has a therapeutic effect on osteoporosis, and to be put to practical use in the future osteoporosis clinic.

Differential Regulation of Obesity by Swim Training in Female Sham-operated and Ovariectomized Mice

  • Jeong, Sun-Hyo;Yoon, Mi-Chung
    • Biomedical Science Letters
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    • v.17 no.1
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    • pp.13-20
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    • 2011
  • The peroxisome proliferator-activated receptor ${\alpha}$ ($PPAR{\alpha}$) is a nuclear transcription factor that plays a central role in lipid and lipoprotein metabolism. To investigate whether swim training improves obesity and lipid metabolism through $PPAR{\alpha}$ activation in female sham-operated (Sham) and ovariectomized (OVX) mice, we measured body weight, visceral adipose tissue mass, serum free fatty acid at 6 weeks as well as the expression of hepatic $PPAR{\alpha}$ target genes involved in fatty acid oxidation. Swim-trained mice had decreased body weight, visceral adipose tissue mass and serum free fatty acid levels compared to high fat diet fed control mice in both female Sham and OVX mice. These reductions were more prominent in OVX than in Sham mice. Swim training significantly increased hepatic mRNA levels of $PPAR{\alpha}$ target genes responsible for mitochondrial fatty acid ${\beta}$-oxidation, such as carnitine palmitoyltransgerase-1 (CPT-1), very long chain acyl-CoA dehydrogenase (VLCAD), and medium chain acyl-CoA dehydrogenase (MCAD) in OVX mice. However, swim trained female Sham mice did not increase hepatic mRNA levels of $PPAR{\alpha}$ target genes responsible for mitochondrial fatty acid ${\beta}$-oxidation compared to Sham control mice. These results indicate that swim training differentially regulates body weight and adipose tissue mass between OVX and Sham mice, at least in part due to differences in liver $PPAR{\alpha}$ activation.

Effects of Herbal-Acupuncture with Cistanches Herba Extract at KI10 (Eumgok) on Osteoporosis in Ovariectomized Mice (음곡 육종용 약침이 난소적출 생쥐의 골다공증에 미치는 영향)

  • Jeung, Neon-Sik;Lee, Byung-Ryul;Koo, Sung-Tae;Yim, Yun-Kyoung
    • Korean Journal of Acupuncture
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    • v.25 no.3
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    • pp.97-115
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    • 2008
  • Objective & Methods : This study aimed to evaluate the effects of Cistanches Herba herbal-acupuncture (CH-HA) at KI10 (Umgok) on osteoporosis induced by ovariectomy in mice. The results were as follows; Results : CH-HA at KI10 significantly inhibited the overgrowth of tibia, the elevation of serum osteocalcin level, the reduction of tibial Ca and P level, the increase of tibial osteoclast like cells, the reduction of tibial TBV (trabecular bone volume), the overgrowth of tibial GPL (growth plate length) in ovariectomized mice. NP at KI10 significantly inhibited the reduction of BMD (bone mineral density) in ovariectomized mice, but CH-HA did not. Conclusion : Taken together, CH-HA at KI10 has a protective or therapeutic effect for osteoporosis in ovariectomized mice. Therefore, it is suggested that CH-HA may be an useful therapeutics in clinical filed after further researches.

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Effects of the Herbal-Acupuncture with Lycii Fructus Extract at KI10 (Eumgok) on Osteoporosis in Ovariectomized ddy mice (음곡(KI10) 구기자 약침이 난소적출 생쥐의 골다공증에 미치는 영향)

  • Hwang, Gyu-Tae;Lee, Hyun
    • Korean Journal of Acupuncture
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    • v.26 no.1
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    • pp.139-159
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    • 2009
  • Objective & Methods : The purpose of this study is to observe the effects of herbal-acupuncture with Lycii Fructus (LF-HA) at KI10 (Eumgok) on osteoporosis in ovariectomized (OVX) ddy mice. We carried out several experimental items which were known to be related with osteoporosis. Results : 1. Tibial BMD was significantly increased in the LF-HA group. 2. Tibial Ca level was significantly increased in the LF-HA group. 3. Osteoclast like cell in tibia was significantly decreased in the LF-HA group. 4. Histological change of tibia was improved, the trabecular bone thickness was significantly increased and the growth plate length was significantly decreased in the LF-HA group. Conclusion : These results suggest that LF-HA at KI10 has therapeutic effects on osteoporosis especially by way of suppressing osteoclast cells in ovariectomized ddy mice.

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Effects of Schisandrae Fructus on menopause symptoms in ovariectomized mice (난소를 절제한 마우스에서 갱년기증후군에 대한 오미자의 효과)

  • Shin, Dasom;Hong, Seong Bin;Geum, Jeong Ho;Ma, Jin Yeul;Chung, Hwan-Suck
    • The Journal of Korean Medicine
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    • v.37 no.3
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    • pp.39-46
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    • 2016
  • Objectives: The present study aimed at investigating the effects of Schisandrae Fructus (SF) on menopause symptoms in ovariectomized mice. Methods: 6 weeks old female mice were ovariectomized (OVX), and the OVX mice were fed with a three doses of SF (25, 100, and 400 mg/kg, respectively) or $17{\beta}$-estradiol (E2, 0.5 mg/kg) 3 times a week for 6 weeks. Body weight was measured at every week and uterus weight was also measured at the end of experiment. Lipids and osteocalcin levels in serum were analyzed. Results: The uterine weight was not changed in SF treated group, while E2 intake increased the reduced uterine weight by OVX. Although Body weight was increased since two weeks after ovariectomy compared with sham operated group, there was no change in SF or E2 treated group compared with OVX. But LDL was significantly reduced in SF (400 mg/kg) or E2 fed mice and SF or E2 treatment decreased enhanced osteocalcin level by OVX. Conclusion: These results indicated that SF would be effective in the improvement of LDL level and osteoporosis on menopause.

Difference in the Gut Microbiome between Ovariectomy-Induced Obesity and Diet-Induced Obesity

  • Choi, Sungmi;Hwang, Yu-Jin;Shin, Min-Jeong;Yi, Hana
    • Journal of Microbiology and Biotechnology
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    • v.27 no.12
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    • pp.2228-2236
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    • 2017
  • During menopausal transition, the imbalance of estrogen causes body weight gain. Although gut microbiome dysbiosis has been reported in postmenopausal obesity, it is not clear whether there is any difference in the microbiome profile between dietary-induced obesity and postmenopausal obesity. Therefore, in this study, we analyzed intestinal samples from ovariectomized mice and compared them with those of mice with high-fat diet-induced obesity. To further evaluate the presence of menopause-specific bacteria-gene interactions, we also analyzed the liver transcriptome. Investigation of the 16S rRNA V3-V4 region amplicon sequence profile revealed that menopausal obesity and dietary obesity resulted in similar gut microbiome structures. However, Bifidobacterium animalis was exclusively observed in the ovariectomized mice, which indicated that menopausal obesity resulted in a different intestinal microbiome than dietary obesity. Additionally, several bacterial taxa (Dorea species, Akkermansia muciniphila, and Desulfovibrio species) were found when the ovariectomized mice were treated with a high-fat diet. A significant correlation between the above-mentioned menopause-specific bacteria and the genes for female hormone metabolism was also observed, suggesting the possibility of bacteria-gene interactions in menopausal obesity. Our findings revealed the characteristics of the intestinal microbiome in menopausal obesity in the mouse model, which is very similar to the dietary obesity microbiome but having its own diagnostic bacteria.