• Title, Summary, Keyword: pathogenesis

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A Study of Pathogenesis Classification using Decision Tree Method (의사결정나무법을 이이용한 병인(病因)분류에 관한 연구)

  • Lee, Hyuk-Jae;Kim, Min-Yong;Oh, Hwan-Sup;Park, Young-Bae
    • The Journal of the Korean Medicine Diagnostics
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    • v.12 no.2
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    • pp.27-40
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    • 2008
  • Background : In spite of the predominant of the theory of Pathogenesis, the method of Pathogenesis classification is depending on the doctor's clinical trials because od the lack of the objective test criteria. Methods and Results : This study is trying to improve the objectiveness of classification using a new statistical method, decision tree. Decision tree method -a classification technique in the statistical analysis- was used to analyze the result of pathogenesis questionnaire instead of using discriminant analysis. As a result, 10 among 38 pathogenesis questionnaire was selected as important questions and 12 terminal nodes was built to classify the pathogenesis. Conclusions : Using only 10 questions shown in the result of decision tree, we can classify and interpret the pathogenesis easily and effectively.

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Delineating Transcription Factor Networks Governing Virulence of a Global Human Meningitis Fungal Pathogen, Cryptococcus neoformans

  • Jung, Kwang-Woo;Yang, Dong-Hoon;Maeng, Shinae;Lee, Kyung-Tae;So, Yee-Seul;Hong, Joohyeon;Choi, Jaeyoung;Byun, Hyo-Jeong;Kim, Hyelim;Bang, Soohyun;Song, Min-Hee;Lee, Jang-Won;Kim, Min Su;Kim, Seo-Young;Ji, Je-Hyun;Park, Goun;Kwon, Hyojeong;Cha, Sooyeon;Meyers, Gena Lee;Wang, Li Li;Jang, Jooyoung;Janbon, Guilhem;Adedoyin, Gloria;Kim, Taeyup;Averette, Anna K.;Heitman, Joseph;Cheong, Eunji;Lee, Yong-Hwan;Lee, Yin-Won;Bahn, Yong-Sun
    • 한국균학회소식:학술대회논문집
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    • pp.59-59
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    • 2015
  • Cryptococcus neoformans causes life-threatening meningoencephalitis in humans, but the treatment of cryptococcosis remains challenging. To develop novel therapeutic targets and approaches, signaling cascades controlling pathogenicity of C. neoformans have been extensively studied but the underlying biological regulatory circuits remain elusive, particularly due to the presence of an evolutionarily divergent set of transcription factors (TFs) in this basidiomycetous fungus. In this study, we constructed a high-quality of 322 signature-tagged gene deletion strains for 155 putative TF genes, which were previously predicted using the DNA-binding domain TF database (http://www.transcriptionfactor.org/). We tested in vivo and in vitro phenotypic traits under 32 distinct growth conditions using 322 TF gene deletion strains. At least one phenotypic trait was exhibited by 145 out of 155 TF mutants (93%) and approximately 85% of the TFs (132/155) have been functionally characterized for the first time in this study. Through high-coverage phenome analysis, we discovered myriad novel TFs that play critical roles in growth, differentiation, virulence-factor (melanin, capsule, and urease) formation, stress responses, antifungal drug resistance, and virulence. Large-scale virulence and infectivity assays in insect (Galleria mellonella) and mouse host models identified 34 novel TFs that are critical for pathogenicity. The genotypic and phenotypic data for each TF are available in the C. neoformans TF phenome database (http://tf.cryptococcus.org). In conclusion, our phenome-based functional analysis of the C. neoformans TF mutant library provides key insights into transcriptional networks of basidiomycetous fungi and ubiquitous human fungal pathogens.

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Association of Mycoplasma pneumoniae in Asthma Pathogenesis

  • Marie, Mohammed Ali M.
    • Tuberculosis and Respiratory Diseases
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    • v.65 no.4
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    • pp.261-268
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    • 2008
  • The role of atypical bacterial infection in the pathogenesis of asthma is a subject of continuing debate. There is an increasing body of literature concerning the association between the atypical bacteria such as Mycoplasma pneumoniae (M. pneumoniae) and asthma pathogenesis. Moreover, many studies investigating such a link have been uncontrolled and have provided conflicting evidence, in part due to the difficulty in accurately diagnosing infection with these atypical pathogens. This manuscript will review the relationship between M. pneumoniae infection and asthma pathogenesis.

Hypoxic Stress Adaptation in Aspergillus nidulans

  • Bat-Ochir, Chinbayar;Koh, Sun-Ki;Kwak, Jun-Yong;Yu, Yeong-Man;Jeon, Mee-Hyang;Maeng, Pil-Jae;Park, Hee-Moon;Han, Dong-Min;Chae, Suhn-Kee
    • Proceedings of the Microbiological Society of Korea Conference
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    • pp.150-151
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    • 2009
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Development of System-Wide Functional Analysis Platform for Pathogenicity Genes in Magnaporthe oryzae

  • Park, Sook-Young;Choi, Jaehyuk;Choi, Jaeyoung;Kim, Seongbeom;Jeon, Jongbum;Kwon, Seomun;Lee, Dayoung;Huh, Aram;Shin, Miho;Jung, Kyungyoung;Jeon, Junhyun;Kang, Chang Hyun;Kang, Seogchan;Lee, Yong-Hwan
    • 한국균학회소식:학술대회논문집
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    • pp.9-9
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    • 2014
  • Null mutants generated by targeted gene replacement are frequently used to reveal function of the genes in fungi. However, targeted gene deletions may be difficult to obtain or it may not be applicable, such as in the case of redundant or lethal genes. Constitutive expression system could be an alternative to avoid these difficulties and to provide new platform in fungal functional genomics research. Here we developed a novel platform for functional analysis genes in Magnaporthe oryzae by constitutive expression under a strong promoter. Employing a binary vector (pGOF1), carrying $EF1{\beta}$ promoter, we generated a total of 4,432 transformants by Agrobacterium tumefaciens-mediated transformation. We have analyzed a subset of 54 transformants that have the vector inserted in the promoter region of individual genes, at distances ranging from 44 to 1,479 bp. These transformants showed increased transcript levels of the genes that are found immediately adjacent to the vector, compared to those of wild type. Ten transformants showed higher levels of expression relative to the wild type not only in mycelial stage but also during infection-related development. Two transformants that T-DNA was inserted in the promotor regions of putative lethal genes, MoRPT4 and MoDBP5, showed decreased conidiation and pathogenicity, respectively. We also characterized two transformants that T-DNA was inserted in functionally redundant genes encoding alpha-glucosidase and alpha-mannosidase. These transformants also showed decreased mycelial growth and pathogenicity, implying successful application of this platform in functional analysis of the genes. Our data also demonstrated that comparative phenotypic analysis under over-expression and suppression of gene expression could prove a highly efficient system for functional analysis of the genes. Our over-expressed transformants library would be a valuable resource for functional characterization of the redundant or lethal genes in M. oryzae and this system may be applicable in other fungi.

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Recent progress in the understanding of clinical characteristics, epidemiology, and pathogenesis of new bronchopulmonary dysplasia (새로운 유형의 기관지폐이형성증의 임상적 특성, 역학, 병리기전에 대한 국내외 최근 연구동향)

  • Kim, Beyong Il
    • Clinical and Experimental Pediatrics
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    • v.52 no.1
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    • pp.6-13
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    • 2009
  • Recent advances in perinatal care have resulted in improved survival of extremely low birth weight infants (ELBWI). However, bronchopulmonary dysplasia (BPD) remains one of the major complications in ELBWI. BPD was originally described over 40 years ago; the clinical characteristics, epidemiology, and pathogenesis of BPD have changed markedly through this period. In this article, I have reviewed recent progress in research concerning the clinical presentation and characteristics, epidemiology, and pathogenesis of BPD.

Pathogenesis Study of Oriental OB & GY Questionnaires (한방부인과 진단용 설문지의 병기 연구)

  • Lee In Sun;Jean Ran Hee;Cha Hye Suk;Bae Kyung Mi;Kim Mi Jin;Lee Yong Tae;Ji Gyu Yang;Kim Jong Won
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.2
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    • pp.401-407
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    • 2004
  • Purpose : This study investigated reliability of Oriental DB & GY(obstetrics & gynecology) Questionnaires's items which was used by Dong-Eui OB & GY through analysis of oriental OB & GY books. Method : This study investigated differentiation of syndrome through analysis of oriental OB & GY book's. Result: This study investigated differentiation of syndrome through analysis of oriental OB & GY disease and pathogenesis. This study's pathogenesis was such that deficiency of Ki, deficiency of blood, stagnation of Ki, stagnated blood, deficiency of Yang, deficiency of Um, cold syndrome, heat syndrome, dampness, dryness, phlegm, kidney, liver, heart, spleen, wind, lung. We except lung from Questionnaires's pathogenesis because it is stuck for importance. We except wind from Questionnaires's pathogenesis because it is stuck for preguence. Oriental OB & GY Questionnaires's pathogenesis consist of 15 items such that deficiency of Ki, deficiency of blood, stagnation of Ki, stagnated blood, deficiency of Yang, deficiency of Um, cold syndrome, heat syndrome, dampness, dryness, phlegm, kidney, liver, heart, spleen. Oriental OB & GY Questionnaires construct pathogenesis's question and guide post through we examined it's reasonableness.

Regulation of Pathogenesis by Light in Cercospora zeae-maydis: An Updated Perspective

  • Kim, Hun;Ridenour, John B.;Dunkle, Larry D.;Bluhm, Burton H.
    • The Plant Pathology Journal
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    • v.27 no.2
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    • pp.103-109
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    • 2011
  • The fungal genus Cercospora is one of the most ubiquitous groups of plant pathogenic fungi, and gray leaf spot caused by C. zeae-maydis is one of the most widespread and damaging foliar diseases of maize in the world. While light has been implicated as a critical environmental regulator of pathogenesis in C. zeae-maydis, the relationship between light and the development of disease is not fully understood. Recent discoveries have provided new insights into how light influences pathogenesis and morphogenesis in C. zeae-maydis, particularly at the molecular level. This review is focused on integrating old and new information to provide an updated perspective of how light influences pathogenesis, and provides a working model to explain some of the underlying molecular mechanisms. Ultimately, a thorough molecular-level understanding of how light regulates pathogenesis will augment efforts to manage gray leaf spot by improving host resistance and disease management strategies.

Study on the Tri-origin of Asian Medical History Based on the Contents of Dong-Eui-Bo-Gam ("동의보감(東醫寶鑑).집례(集例)"에 근거한 동아시아 전통의학 역사의 삼원적 구조;한의학과 중의학의 비교 연구)

  • Kim, Byoung-Soo;Kang, Jung-Soo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.4
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    • pp.746-753
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    • 2008
  • East Asian traditional medicine(Asian medicine) should be understood correctly on the basis of Asian medical network. For the correct understanding we analyzed Asian medical history which could be divided into three categories based on Dong-Eui-Bo-Gam. There are three mechanisms of human diseases, which are the pathogenesis of six external etiological factors(wind, cold, summer-heat, dampness, dryness and fire) due to affection by exopathogen [外感六氣], the pathogenesis of five viscera due to internal injury [內傷五藏], and the pathogenesis of physical constitution due to vital activity [生命體質]. While Chinese medicine has mainly developed the pathogenesis of the former two, Korean traditional medicine has mainly developed the pathogenesis of physical constitution.

A Mitochondrial Perspective of Chronic Obstructive Pulmonary Disease Pathogenesis

  • Kang, Min-Jong;Shadel, Gerald S.
    • Tuberculosis and Respiratory Diseases
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    • v.79 no.4
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    • pp.207-213
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    • 2016
  • Chronic obstructive pulmonary disease (COPD) encompasses several clinical syndromes, most notably emphysema and chronic bronchitis. Most of the current treatments fail to attenuate severity and progression of the disease, thereby requiring better mechanistic understandings of pathogenesis to develop disease-modifying therapeutics. A number of theories on COPD pathogenesis have been promulgated wherein an increase in protease burden from chronic inflammation, exaggerated production of reactive oxygen species and the resulting oxidant injury, or superfluous cell death responses caused by enhanced cellular injury/damage were proposed as the culprit. These hypotheses are not mutually exclusive and together likely represent the multifaceted biological processes involved in COPD pathogenesis. Recent studies demonstrate that mitochondria are involved in innate immune signaling that plays important roles in cigarette smoke-induced inflammasome activation, pulmonary inflammation and tissue remodeling responses. These responses are reviewed herein and synthesized into a view of COPD pathogenesis whereby mitochondria play a central role.