• Title, Summary, Keyword: pathogens

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Patterns of mastitic pathogens and antibiotic susceptibility of bovine clinical mastitis (유우의 임상형 유방염 원인균과 항생제 감수성의 변화양상)

  • Kim, Doo
    • Korean Journal of Veterinary Research
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    • v.28 no.2
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    • pp.397-404
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    • 1988
  • A total of 593 mastitic pathogens were isolated from the clinical mastitic milk of dairy cattle in Gyeonggi area from March 1984 to February 1988. The mastitic pathogens were further studied bacteriologically and examined for susceptibility patterns to 10 antibiotics. The results obtained were summerized as follows: 1. One hundred and seventy three pathogens were isolated in the first year of studies, 205 pathogens in the second year, 122 pathogens in the third year and 93 pathogens in the last year. 2. The pathogens isolated from clinical mastitis were more in summer than other seasons. 3. Staphylococcus spp (50%) and Streptococcus spp (18%) were the main pathogens in the first year of studies but coliforms (15%) and other bacteria (40%) were the main pathogens in the last year of studies. 4. Coliform mastitis was occurred only in summer and autumn. 5. Antibiotic susceptibility patterns of the same genuses from clinical mastitis infections in different herds and in different years varied greatly. Therefore, treatment should be selected on the basis of susceptibility test results.

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Growth Inhibition of E. coli O157:H7 and Salmonella typhimurium by Lactic Acid Bacteria and Bifidobacteria (젖산균과 비피더스균에 의한 Escherichia coli O157:H7과 Salmonella typhimurium의 생장억제)

  • 김현욱;안영태;신필기
    • Journal of Food Hygiene and Safety
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    • v.12 no.3
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    • pp.181-187
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    • 1997
  • Lactobacillus acidophilus NCFM, Lactobacillus casei YIT 9018, Bifidobacterium longum 8001, and Bifidobacterium longum 8025 at the level of 106 cfu/$m\ell$ were cultured with 104 cfu/$m\ell$ of Escherichia coli O157:H7 KSC 109 or Salmonella typhimurium ATCC14028, in order to verify the effects of lactic acid bacteria and bifidobacteria on the growth of the pathogens. In the mixed culture of lactic acid bacteria with E. coli O157:H7 KSC 109, Growth inhibition and atypical microcolonies of E. coli O157:H7 KSC 109 were observed. The pathogens inoculated grew for 5 hors (pH 5.3), by the time L. acidophilus NCFM reached the exponential growth phase, and then the surviving pathogens were decreased to 101 cfu/$m\ell$ after 35 hours. When L. caseiYIT 9018 was grown with the pathogens, they grew for 10 hours (pH 4.6), by the time L. casei YIT 9018 reached the end of exponential growth phase, and then the surviving pathogens were decreased drastically. Up to the stationary growth phase of lactic acid bacteria, L. acidophilus NCFM exhibited stronger inhibition against the pathogens than L. casei YIT 9018 did, which might be attributed to its faster growth. Likewise bifidobacteria inhibited the growth of the pathogens tested, bifidobaceria was weaker in the inhibitory activity than lactic acid bacteria. When Bifidobacterium longum 8001 was cultured with the pathogens, E. coli O157:H7 KSC 109 was gradually ingibited at the stationary growth phase of bifidobacteria, atypical microcolonies were formed on Levine EMB medium after 48 hours, and Salmonella grew up to 106 dfu/$m\ell$, then was drastically ingibited at the exponential growth phage of Bifidobacterium longum 8001. But when Bifidobacteriuam longum 8025 was cultured with the pathogens, the pathogens grew to the same level of Bifidobacteriuam longum 8025 was cultured with the pathogens, the pathogens grew to the same lever of Bifidobacteriuam longum 8025 after 10 hours, then the surviving pathogens were decreased drastically.

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Detection of Respiratory Viral Pathogens and Mycoplasma spp from Calves with Summer Pneumonia in Korea

  • Park, Jung-hoon;Kim, Doo
    • Journal of Veterinary Clinics
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    • v.36 no.4
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    • pp.185-189
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    • 2019
  • Respiratory pathogens of calves including bovine parainfluenza type 3 virus (BPI3V), bovine respiratory syncytial virus (BRSV), infectious bovine rhinotracheitis virus (IBRV) and Mycoplasma spp is well-known for winter pathogens. However, there are no studies about summer pneumonia pathogens of calves in Korea. The aim of this study was to detect respiratory pathogens from calves with summer pneumonia. Eighty calves from 5 regions were chosen and their nasal swabs were used to detect respiratory pathogens with real-time PCR. Mycoplasma spp was major primary respiratory pathogens in calves with summer pneumonia. Although, the detection rates of respiratory viruses were very low, serological assays showed that respiratory viruses exist widely in farms.

The Interaction of Human Enteric Pathogens with Plants

  • Lim, Jeong-A;Lee, Dong Hwan;Heu, Sunggi
    • The Plant Pathology Journal
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    • v.30 no.2
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    • pp.109-116
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    • 2014
  • There are an increasing number of outbreaks of human pathogens related to fresh produce. Thus, the growth of human pathogens on plants should be explored. Human pathogens can survive under the harsh environments in plants, and can adhere and actively invade plants. Plant-associated microbiota or insects contribute to the survival and transmission of enteric pathogens in plants. Human enteric pathogens also trigger plant innate immunity, but some pathogens-such as Salmonella-can overcome this defense mechanism.

Disinfectant Effects of Ecological Familiar Surfactant against Fish Pathogens (환경친화성 계면활성제를 이용한 어병균의 살균효과)

  • 최상원
    • Journal of Environmental Science International
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    • v.9 no.5
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    • pp.365-368
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    • 2000
  • The antibacterial effect amino acid-copper(II) surfactant on fish pathogens was studied. Fish pathogens of Edwardsiella tarda Vibrio anguillarum Aeromonas hydrophila and Streptococcus sp. were selected cultured in nutrient agar and adjusted at $2{\times}10^5~10^6 CFU/$m\ell$$ in phosphate buffer saline before the addtion of amino acid-copper(II) surfactant with different concentrations. All tested pathogens died within 1 hour with 1 ppm of amino acid-copper(II) surfactant. In comparison with formalin and ET. amino acid-copper (II) surfactnat was more effective in antibacterial capacity.

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Weissella confusa Strain PL9001 Inhibits Growth and Adherence of Genitourinary Pathogens

  • Lee, Yeon-Hee
    • Journal of Microbiology and Biotechnology
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    • v.14 no.4
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    • pp.680-685
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    • 2004
  • The capability of lactic acid bacteria (LABs) to adhere to intestinal epithelial cells and vaginal epithelial cells is an important factor in the formation of a barrier to prevent the colonization of pathogenic bacteria. In addition, the ability to coaggregate with pathogens and production of antimicrobial agents also allow LABs to fight against pathogens. In this work, Weissella confusa PL9001 was tested for its ability to inhibit the growth and adherence of genitourinary pathogens, including Candida albicans, Escherichia coli, Staphylococcus aureus, and vancomycin-resistant Enterococcus faecium (VRE), isolated from the urine of hospitalized female patients. W. confusa PL9001 was found to coaggregate with the four pathogens, as observed with a light microscope and scanning electron microscope. In competition, exclusion, and displacement tests, the adherence of the pathogens to T24 bladder epithelial cells was also inhibited by W. confusa PL9001. Accordingly, these results suggest that W. confusa PL9001 is potentially useful for both preventive and therapeutic treatment of genitourinary infections.

Comparison of quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction

  • Kim, Young-Sun;Lee, Jung-Hwa;Lee, Young-Eun
    • Journal of Korean society of Dental Hygiene
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    • v.15 no.6
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    • pp.1063-1071
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    • 2015
  • Objectives: The purpose of the study is to investigate the quantitative detection of periodontal pathogens before and after scaling by real-time polymerase chain reaction. Methods: Participants were voluntarily recruited at D university, and saliva samples were extracted before and after scaling. Multiple real-time polymerase chain reactions were used to analyze characteristics and the amount of nine kinds of periodontal pathogens; Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, Fusobacterium nucleatum, Parvimonas micra, Campylobacter rectus, and Eikenella corrodens. Results: After scaling, most periodontal pathogens except Eikenella corrodens were significantly decreased in all subjects(p<0.05). In addition, the percentage of microorganisms associated with disease, the microorganism risk index of periodontitis and the prevalence of red complex, orange complex, and Aggregatibacter actinomycetemcomitans was also significantly reduced after scaling(p<0.05). Conclusions: Scaling decreased in the amount of major periodontal pathogens and periodontitis prevalence rate.

High Incidence of Staphylococcus aureus and Norovirus Gastroenteritis in Infancy: A Single-Center, 1-Year Experience

  • Sung, Kyoung;Kim, Ji Yong;Lee, Yeoun Joo;Hwang, Eun Ha;Park, Jae Hong
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.17 no.3
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    • pp.140-146
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    • 2014
  • Purpose: The etiology of acute gastroenteritis (AGE) has changed since the introduction of the rotavirus vaccination. The aim of this study was to clarify which common pathogens, both bacterial and viral, are currently causing AGE in infants. Methods: Infants with acute diarrhea were enrolled. We tested for 10 bacterial pathogens and five viral pathogens in stool specimens collected from infants with AGE. The clinical symptoms such as vomiting, mucoid or bloody diarrhea, dehydration, irritability, and poor oral intake were recorded, and laboratory data such as white blood cell count and C-reactive protein were collected. The clinical and laboratory data for the cases with bacterial pathogens and the cases with viral pathogens were compared. Results: Of 41 total infants, 21 (51.2%) were positive for at least one pathogen. Seventeen cases (41.5%) were positive for bacterial pathogens and seven cases (17.1%) were positive for viral pathogens. Staphylococcus aureus (13 cases, 31.7%) and Clostridium perfringens (four cases, 9.8%) were common bacterial pathogens. Norovirus (five cases, 12.2%) was the most common viral pathogen. Fever and respiratory symptoms were common in the isolated viral infection group (p=0.023 and 0.044, respectively), whereas other clinical and laboratory data were indistinguishable between the groups. Conclusion: In our study, S. aureus (41.5%) and norovirus (12.2%) were the most common bacterial and viral pathogens, respectively, among infants with AGE.