• Title, Summary, Keyword: pectic substances

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Production of Pectolytic Enzymes and Change of Pectic Substances from Apple Fruits Infected with Botryosphaeria dothidea (사과 겹무늬썩음병균(Botryosphaeria dothidea)에 의해 부패된 사과 과실에서 Pectin질 분해효소의 생산과 Pectin질의 변화)

  • Park, Seok-Hee;Lee, Chang-Un
    • The Korean Journal of Mycology
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    • v.21 no.2
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    • pp.106-111
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    • 1993
  • Pectolytic enzymes were extracted in apple fruits rotted by Botryosphaeria dothidea, and their activities and change of pectic substances were investigated. Exo-polygalacturonase(exo-PG), exo-polymethylgalacturonase(exe-PMG), polygalacturonate-trans-eliminase(PGTE) and pectin-methyl-trans-eliminase(PMTE) were produced by the pathogen. Activities of exo-PG and exe-PMG extracted from rotten apple fruits were high to 21.15 and 24.65 units/mg protein in specific activity at seven days after inoculation, respectively. Activities of PGTE and PMTE showed 5.60 and 7.90 units/mg protein, respectively, but they were lower than those of the exo-type enzymes. Water-soluble and versene-soluble pectins were 11.50 mg/100 mg-AIS and 7.31 mg/100 mg-AIS at 14 days after inoculation, namely, they were increased by 4.23 and 2.16 mg/100 mg-AIS over those of sound apples, respectively. Total soluble pectic substances of rotten apple were 72.4% of total pectic substances and it was higher by 24.8% than sound apple. Insoluble pectic substance was notably decreased from 15.32 to 7.16 mg/100 mg-AIS according to progress of decay while total pectic substances were not changed remarkably.

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Changes in the Pectic Substance during Ripening of Salted Cucumber Pickle (염지 오이피클의 숙성중 펙틴질의 변화)

  • 오영애;이만정;김순동
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.19 no.2
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    • pp.143-150
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    • 1990
  • The changesin hardness activities of pectinestrase and polygaacturonase and amounts of pectic substances of cucumber during salting at 1$0^{\circ}C$ were investigated, The hardness of the cucumber was decreased dramatically after 3 weeks whereas activities of pectinestrase and polygalacturonase were increased until 3 weeks and 2 weeks respectively and then decrea-sed. The level of alcohol insoluble solid and protopectin in the cucumber were decreased but those of pectic acid and water soluble pectin were increased during the whole salting periods, Protopectin fractionated from alcohol insoluble solid during salting of cucumber was separated using Sephacryl S-500 It showed that high average molecular weight(AMW) of 100,000 was decreased however lower molecular weight compounds was increased. Pectic acid was observed to be decomposed from AMW 200,000 to AMW 500,000 Water soluble pectin from fresh cucumber contained higher level of pentose with peak I of AMW 2,000,000, however after 6 weeks of saltinf peak II which represented AMW 100,000 was separated. From the changes of sugar composition, the phenomena of softness during the salting was probably caused by solubilization of hemicellulose associated with pectin and decomposition of pectic substances.

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Changes in the Components of Cell Wall of Persimmon Fruit by Treatments of Cell Wall-Degrading Enzymes (세포벽 분해효소의 처리에 따른 감과실의 세포벽 성분의 변화)

  • 김광수;신승렬;송준희;김주남
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.2
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    • pp.242-246
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    • 1995
  • This paper was carried out to investigate changes in cell wall, cell wall polysaccharides, pectic substances extracted from cell wall of persimmon fruits treated with polygalacturonase and $\beta$-galactosidase in vitro. Degrading degree of cell wall treated with cell wall-degrading enzymes were higher in order polygalacturonase, polygalacturonase+$\beta$-galactosidase and $\beta$-galactosidase. Contents of soluble pectic substances in cell wall treated with cell wall-degrading enzymes showed as the same order as degrading degree of cell wall, while contents of insoluble pectin lower. Contents of versene-soluble pectin and total pectic substance were not affected by cell wall-degrading enzymes. Contents of uronic acid and hexose in soluble material isolated from cell wall treated with polygalacturonase and mixed enzyme were higher than those of untreatment and $\beta$-galactosidase treatment.

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The Changes of Pectic Substances in Sweet Potato Cultivars During Baking (가열에 의한 고구마 펙틴질의 변화)

  • Lee, Kyeong-Ae;Shin, Mal-Shick;Ahn, Seung-Yo
    • Korean Journal of Food Science and Technology
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    • v.17 no.6
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    • pp.421-425
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    • 1985
  • The changes of pectic substances during baking at $175{\sim}180^{\circ}C$ for 30 minutes and polygalacturonase activities of three sweet potato cultivars on textural properties were investigated. The three different types of sweet potato cultivars were the dry type (Wonki), the intermediate type (Shinmi), and the moist type (Chunmi). The moisture content, alcohol insoluble solid content, composition of pectic substances and hardness were determined before and after baking. It was found that HCl-soluble pectin was significantly changed into water soluble and sodium hexametaphosphate soluble pectin by baking. The dry type, Wonki showed the lowest moisture content, but the highest alcohol insoluble solid content and hardness. Wonki, whose polygalacturonase activity was the highest, had the highest HCl-soluble pectin content after backing.

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Changes in Mineral and Pectic Substances of Korean Mature-Green Mume (Prunus mume Sieb. et Zucc) Fruits Packaged in Plastic Films with Gas Absorbents during Storage (가스 제거제 첨가에 따른 필름 포장 청매실의 저장 중 무기질 및 펙틴 성분 변화)

  • Cha, Hwan-Soo;Hong, Seok-In;Chung, Myong-Soo
    • Korean Journal of Food Science and Technology
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    • v.35 no.1
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    • pp.149-154
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    • 2003
  • Changes in mineral (Ca, Mg) contents and pectic substances of mature-green 'Nanko' Mume fruits hermetically packaged in 0.03 mm low density polyethylene (LDPE) films with and without gas absorbents were examined during storage at $25^{\circ}C$ for 10 days. Each packaging contained 10 g $Ca(OH)_2$ as a carbon dioxide scavenger, 30 g $KMnO_4$ as an ethylene scrubber or their mixture. In the presence of the ethylene scrubber, losses in mineral contents of alcohol insoluble solids and water soluble pectin were remarkably suppressed, whereas no significant difference was observed in the Ca content between the fresh fruit and those stored for 10 days. Fruits packaged with the ethylene absorbent retained higher amount of pectic substances than those with other packaging treatments. Degradation of the pectic substances into small molecules was also noticeably reduced when the ethylene scrubber was used. Overall results showed that the combination of the gas permeable film and the ethylene absorbent could be applied to mature-green Mume fruits as an effective packaging method to retard the texture softening during storage at the ambient temperature.

Effect of Starch and Pectic Substances on Potato Texture (전분과 펙틴질이 감자의 텍스쳐에 미치는 영향)

  • 이진희;이혜수
    • Korean journal of food and cookery science
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    • v.5 no.1
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    • pp.43-47
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    • 1989
  • For identifying the cause of the difference from the texture of mealy potatoes-Namjak and soomi- and soggy Potatoes-Daeji and Dowon-, starch and pectic substances and their relating materials were analyzed, and polygalacturonase(PG) activity was measured. The shape and size of Namjak and soomi starch granules were circular and diameter ranged from 0.02 to 0.024 mm. In the meanwhile, Daeji and Dowon had two sizes of starch granules; larger one tend to be oval in shape and diameter of longer side ranged from 0.04 to 0.045 mm, that of narrower side ranged from 0.02 to 0.03 mm and smaller one was rather circular in shape and average deameter was below 0.005 mm. Oval shaped potato starch grancules under polarized light showed dark cross of V-shape, whereas circular granules showed cross shape. Total sharch contents of Namjak and soomi were higher than that of Daeji and Dowon. The changes of pectic substances were the decrease of the insoluble pectin and the increase of the soluble pectin, and the reduction of the total pectic substance content after cooking. This phenomena were more prominent in Namjak and Soomi than in Daeji and Sowon. PG activities of Namjak and Soomi were greater than Daeji and Dowon. Calcium contents of Namjak and Soomi were lower than Daeji and Dowon. Phytic acid contents of Namjak, Soomi, Daeji and Dowon were 0.093%, 0.096%, 0.078%, and 0.081%, respectively. Hardness of Namjak and Soomi were less than Daeji and Dowon. The mealy potatoes had higher starch contents, higher tendency to pectin solubilization, higher PG activities, lower calcium contents and less hardness than the soggy Potatoes.

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Physicochemical Properties and Intestinal Bacterial Growth-Promoting Effect of Cell-Wall Polysaccharides from Cucumber Peel

  • Jun, Hyun-Il;Song, Geun-Seoup;Lee, Young-Tack;Kim, Young-Soo
    • Food Science and Biotechnology
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    • v.14 no.3
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    • pp.375-379
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    • 2005
  • Physicochemical properties, intestinal microbial growth, and inhibitory effects of alcohol-insoluble polysaccharide (AIP) extracted from cucumber peel were investigated. AIP was composed of 14.54% crude protein, 1.04% crude lipid, 13.74 % crude ash, 9.1% soluble dietary fiber, and 41.2% insoluble dietary fiber. AIP showed low bulk density (0.18 g/mL) and water-holding capacity (6.39 g/g), and high oil-holding capacity (3.96 g/g). Pectic substance fractions [water-soluble pectic substance (WSP), ethylenediaminetetraacetic acid-soluble pectic substance (ESP), and alkali-soluble pectic substances (ASP)] and hemicellulose fractions [1 M KOH-soluble hemicellulose (KHP1) and 4 M KOH-soluble hemicellulose (KHP4)] were obtained from sequential chemical fractionation of AIP. WSP showed higher total sugar contents than total uronic acid contents, whereas opposite results were observed in ESP and ASP. Molecular weight distributions of three pectic substance fractions were in order of ASP>ESP>WSP. Ion exchange chromatogram pattern of WSP was different from those of ESP and ASP. Major component of WSP was fraction eluted by 0.05 M ammonium acetate buffer, whereas that of ESP and ASP was fraction eluted by 0.2 M NaOH. WSP and ASP showed growth-promoting activities against Lactobacillus brevis, Bifidobacterium bifidum, and B. longum, whereas B. bifidum and B. longum for ESP. KHP1 and KHP4 fractions had significant growth-promoting activities against B. bifidum.

Effect of Various Cooking Method on Pectin in Vegetables (여러 가지 조리방법에 따른 펙틴 함량의 변화)

  • Gye, Su-Gyeong
    • Journal of Applied Tourism Food and Beverage Management and Research
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    • v.17 no.2
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    • pp.27-38
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    • 2006
  • The contents and physicochemical properties of fiber in 15 kinds of vegetables being consumed commoly in Korea were investigated, and the effects of various cooking and Kimchi fermentation) on fiber were studied. Contents of total pechin in raw vegetables were 0.89-2.75g/100g, and most were 1-2g/100g, on dry weight basis. Considering each fraction of pectic substances, the content of HWSP of vegetables ranged in 0.33-0.98g/100g, HXSP, 0.29-0.81g/100g and HCLSP, 0.30-1.40g/100g. HCLSP of three pectic fractions showed the greatest variation according to the kinds of vegetables. Every vegetables showed similar contents of three pectic fraction. Cooking decreased total pectin content in all vegetables regardless of cooking methods and the greatest reduction was observed in boiling. Among three pectic fractions, hot water soluble pectin(HWSP) increased, and sodium hexamtaphosphate soluble pectin(HXSP) and HCL soluble pectin(HCLSP) decreased.

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Changes in the Pectic Substance during Maturity of Apple (사과의 성숙에 따른 Pectin질의 변화)

  • Kim, Yeung-Ji;Kim, Chang-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.17 no.1
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    • pp.63-68
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    • 1988
  • The changes of methoxy content. compositional sugars. degree of esterification (DE) and pectic substances such as LMP (low methoxy pectin), HMP (high methoxy pectin) IAP (ionically associated pectin) and CBP (covalently bounded pectin) of Fuji apple were investigated during maturity. The contents of LMP+HMP and IAP+CBP in alcohol insoluble solid(AIS) were 70% and 20%. respectively. These pectic substances were increased slightly during maturity. The reductions of hexose and uronic acid were Iron 20-50% and from 2-4%, respectively, but the inclosed of pentose was from 1-9% in LMP, HMP, IAP and CBP during maturity of Fuji apple. The galactose was decreased significantly among hexose. Whereas the relative ratio of uronic acid in HMP and CBP was increased.

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Changes of Non-Cellulosic Neutral Sugars of Cell Wall in Soybean Sprouts (콩나물 생장중 세포벽 비섬유성 중성당의 변화)

  • 신승렬;박찬성;김주남;김광수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.6
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    • pp.1041-1046
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    • 1998
  • This study was carried out to investigate the changes and composition of the non-cellulosic neutral sugars in cell wall of soybean sprouts during growth. The composition of non-cellulosic neutral sugars in cell of soybean sprouts was rhamnose, fucose, xylose, arabinose, mannose, galactose and glucose. The galactose content of cell wall was higher than other non-cellulosic neutral sugars, and was remarkably decreased during growth. The major non-cellulosic sugars of pectic substances were rhamnose, arabinose, and galactose. The arabinose content of pectic substance was increased in cotyledon and hypocotyl during growth. The contents of non-cellulosic neutral sugars were decreased in hypocotyl during growth. The galactose content of pectic substance was higher in cotyledon than those in hypocotyl, and was increased in cotyledon. The content of rhamnose was higher in ionically associated pectic substance than that in covalently bounded pectic substance. The major non-cellulosic neutral sugars of hemicellulose were glucose, rhamnose, arabinose and galactose. The galactose of hemicellulose was decreased remarkably during growth.

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