• Title, Summary, Keyword: pigment

Search Result 1,763, Processing Time 0.065 seconds

Influence of Orgnainc Pigment Addition on Surface Properties of Coating Layer (유기안료의 첨가가 도공층의 표면특성에 미치는 영향)

  • 정경모;이용규
    • Journal of Korea Technical Association of The Pulp and Paper Industry
    • /
    • v.36 no.2
    • /
    • pp.47-53
    • /
    • 2004
  • In this experiment the effects of the packing structure of pigment on the surface characteristics (smoothness and gloss) of coated paper are studied. Four different kinds of inorganic pigments(clay), ground calcium carbonate(GCC), two of precipitated calcium carbonates(PCC), and two organic pigments(solid bead and hollow type) were used. The method of measuring the relative sediment volume(RSV) was used to analyze the packing structure of coating layer. The relative sediment volume was measured, using the pressure dewatering dry-cake method(PDDM) and centrifuge method. Also, the particle size distribution of coating pigment was determined. The results showed that small amount of organic pigment, added to inorganic pigment, improved smoothness and its effect was greater when GCC was used as inorganic pigment. The efficiency of organic pigment depended upon the inorganic pigment since the organic pigment is packed in the pores formed by the inorganic pigment.

Isolation of Pigment Overproducing Mutant from Monascus purpureus and Optimization of Pigment Production (Monascus purpureus로부터 다량의 색소생성 변이주의 분리 및 색소생성의 최적조건)

  • Park Chi Duck;Jung Hyuck Jun;Yu Tae Shick
    • Korean Journal of Microbiology
    • /
    • v.41 no.2
    • /
    • pp.130-134
    • /
    • 2005
  • Isolation of a pigment overproducing mutant, P-57, by ultraviolet irradiation of Monascus purpureus KCCM 60016 and investigation of the optimal conditions for pigment production of the mutant were carried out. P-57 mutant produced pigment on solid state culture. Unpolished rice was the best cereal source for pigment production among eight kinds of tested cereal sources for the solid culture of the mutant. The optimal culture condition for pigment production were obtained from the cultivated at $30^{\circ}C,\;90\%$ humidity for 30 days. The P-57 mutant strain showed the best pigment productivity of 160.0 unit at red pigment, 193.6 unit at orange pigment, and 141.6 unit at yellow pigment on solid state culture under optimal condition.

Formation of Sensory Pigment Cells Requires Fibroblast Growth Factor Signaling during Ascidian Embryonic Development

  • Kim, Gil-Jung
    • Animal cells and systems
    • /
    • v.7 no.3
    • /
    • pp.221-225
    • /
    • 2003
  • The tadpole larva of the ascidian Halocynthia roretzi has two sensory pigment cells in its brain vesicle. To elucidate the temporal requirement for FGF signaling in formation of the pigment cells, embryos were treated with an FGF receptor 1 inhibitor, SU5402, or an MEK inhibitor, U0126 during various embryonic stages. In the present study, it is shown that the embryos treated with SU5402 from the 16-cell stage to the early gastrula stage do not form pigment cells, whereas those treated after the early gastrula stage form pigment cells. In pigment cell formation, embryos suddenly exhibited the sensitivity to SU5402 only for 1 h at the neural plate stage(-4 h after the beginning of gastrulation). When U0126 treatment was carried out at various stages between the 8-cell and late neurula stages, the embryos scarcely formed pigment cells. Pigment cell formation occurred when the embryos were placed in U0126 at early tail bud stage. These results indicate that FGF signaling is involved in pigment cell formation at two separate processes during ascidian embryogenesis, whereas more prolonged period is required for MEK signaling.

Binding Capacity of Chitin and Chitosan to Anthocyanin Pigment Isolated from Purple Perilla Leaves

  • Chang, Eun-Ju;Park, Sang-Won;No, Hong-Kyoon
    • Preventive Nutrition and Food Science
    • /
    • v.5 no.1
    • /
    • pp.1-6
    • /
    • 2000
  • The binding capacity of chitin and chitosan to anthocyanin pigmentss isolated from purple perilla leaves was inves-tigated. The pigment binding capacity increased with increasing pigment concentrations and decreasing pH without being affected by reaction temperature and particle sizes. Regression analysis revealed significantly high corre-lations between pigment binding capacity of chitin and chitosan and pigment concentration at ranges of 25-100 mg of pigment/g of sample, After 1 hr settling, release of pigment from pigmented chitin and chitosan increased with increasing pH, up to 24.9% and 17.4%, respectively, at pH 9. In general, pigment binding capacity of chitosan was higher than that of chitin. There results suggest that chitosan may be useful as a potential adsorbent capable of stabilizing anthocyanin pigment.

  • PDF

Studies on the Red Pigment Produced by Monascus sp. in Submerged Culture. Part II Production of Crude Pigment, Physical and Physiological Characteristics. (액침 진탕 배양에 의한 Monascus sp.가 생산하는 적색 색소에 관한 연구 제2보 적색조색소의 생산과 물리적 성질 및 생리적 성질)

  • 김현수;곽효성;양호석;변유량;유주현
    • Microbiology and Biotechnology Letters
    • /
    • v.7 no.1
    • /
    • pp.31-36
    • /
    • 1979
  • Yellow pigment was extracted by petroleum ether, and red pigment by 60% ethanol. By thin layer chromatography red pigment preparation consists of more than five species whereas yellow pigment preparation consist of single species. The absorption curve of pigment solution exhibits maximum peak at wavelongth range of 495~500 nm and endo pigment at 394~403 nm. Pigment solution was relatively stable at the pH range of 3~9. Extracted pigment solution gave negative hemolysis test and pigment showed no bio-activity and nontoxicity.

  • PDF

Characterization of Red Pigment Production by Monascus anka (Monasucs anka의 적색조 생산 특성)

  • 김희구;박근태;손홍주
    • The Korean Journal of Food And Nutrition
    • /
    • v.11 no.6
    • /
    • pp.612-616
    • /
    • 1998
  • Optimal media and cultural conditions for the production of red pigment were established using Monasurs anka KFCC 4478. The optimal temperature and initial pH for the production of red pigment were 30$^{\circ}C$ and 7.0, respectively. Glucose turned out to be most suitable carbon source for red pigment production. Optimal glucose concentration was 3.0%. Addition combined of nitrogen sources of peptone and NaNo3 induced good red pigment production. Thiamine-HCI and nicotinic acid were increased the production of red pigment. Under optimal conditions, maximum red pigment production and cell growth were observed after 5 days of incubation.

  • PDF

Partial Purification and Stability of a Water-soluble Yellow Pigment from Bacillus sp. PY123. (Bacillus sp. PY123 균주가 생성하는 수용성 황색수소의 부분 정제 및 그 안정성)

  • 김지연;김광현;김병우;이광배
    • Journal of environmental and Sanitary engineering
    • /
    • v.14 no.4
    • /
    • pp.35-40
    • /
    • 1999
  • For application of a yellow pigment as food additives, stability of a water-soluble yellow pigment from Bacillus sp. PY123 was investigated. The yellow pigment from Bacillus sp. PY123 was purified with pH treatment, activated carbon and silica gel column chromatography. The partial purified yellow pigment appeared only one spot on silica gel TLC after 12 evaporation and under irradiation of UV 253nm at dark room. Rf value of the pigment was measured at 0.04 and 0.12 with development of a solvent mixture (Butanol : Acetic acid : water = 4 :1:5) and a solvent mixture (Isopropanol : Ammonia : Water = 9 :1: 2), respectively, The partial purified pigment appeared a white fluorescence under UV365nm irradiation. The partial purified yellow pigment had a main peak and a minor peak on HPLC using 20mM phosphate buffer(pH 7.0) at 1ml/min flow rate. The partial purified pigment was stable at heat treatment, acidic pH, oxide-reductants and surfactants.

  • PDF

Production of Water-Solubled Pigment from Mycelial Culture of Cordyceps scarabaeicola KEFC-C252 and Its Antimutagenic Effect (Cordyceps scarabaeicola KEFC-C252의 균사체 배양에 의한 수용성 색소의 생산과 색소의 항돌연변이 효과)

  • 이현우;손준형;최종환;예병일;신운섭;김중배;김현원
    • Microbiology and Biotechnology Letters
    • /
    • v.28 no.2
    • /
    • pp.111-116
    • /
    • 2000
  • Cultural conditions for the production of water-soluble pigment from mycelial culture of Cordyceps scarabaeicola KEFC-C252 and antimutagenic activity of the pigment were investigated. To obtain the maximum productivity of the pigment from mycelial culture of C. scarabaeicola KEFC-C252, the optimized medium was made with 1.5% sucrose, 2.5% yeast extract and initial pH 5.5. C. scarabaeicola KEFC-C252 was cultivated to reach the maximum concentration of the pigment at $26^{\circ}C$ for 108 hrs. C. scarabaeicola KEFC-C252 produced about 1.2 g/liter pigment under the optimized condition. The pigment was isolated from the culture filtrate by ethylacetate extraction, acidic precipitation and crystallization. The isolated pigment was scarlet hexagonal column crystal, and the color of the pigment was changed according to pH of the solution. The pigment showed violet in the alkaline water but showed red color in the acidic water. The pigment showed inhibitory activity against mutagenic activity induced by 4-nitroquinoline N-oxide. Furthermore, the pigment showed inhibitory activity against spontaneous mutation on Salmonella typhimurium TA98 and TAlOO.

  • PDF

STudies on the Microbial Pigment(I) (미생물의 색소에 관한 연구. 제1보)

  • Ahn, Tae-Seok;Choi, Yong-Keel;Hong, Soon-Woo
    • Korean Journal of Microbiology
    • /
    • v.15 no.4
    • /
    • pp.159-169
    • /
    • 1977
  • The bacteria of red colonies isolated from soil were identified as Serratia marcescens. The best solvent for pigment extraction was n-buthanol and the pigment was identified as prodigiosene. The extracted pigment was stable on temperature and light but not on acidity. The redpigment color changed into red in alkaline solution. The maximum absorbancy of pigment was 466 nm in alkaline condition and 540 nm in acid condition. And the pigment formed single spot on the TLC(starch). By the result of infra red spectrum, the red pigment has the same absorption pattern comparing with, the prodigisin produced by S. marcescens strain Nima. It was confirmed that the pigment was secondary metabolite and that the maximal peak of production appeared at 30 hrs after the inoculation, when the bacterial growth was in statinary state. Referring to the effect of temperature, the pigment was not formed at $36^{\circ}C$ and the optimal temperature for both of bactrial growth and pigmentation was $30^{\circ}C$. The optimal range of pH for pigmentation was 5.0 and under the condition the bacterial growth was not affected at all. Examining the effects of light, the bacterial pigment ation was more increased in darkness than in visible light.

  • PDF

Purification and Physiochemical Characterization of Melanin Pigment from Klebsiella sp. GSK

  • Sajjan, Shrishailnath;Kulkarni, Guruprasad;Yaligara, Veeranagouda;Lee, Kyoung;Karegoudar, T.B.
    • Journal of Microbiology and Biotechnology
    • /
    • v.20 no.11
    • /
    • pp.1513-1520
    • /
    • 2010
  • A bacterium capable of producing melanin pigment in the presence of L-tyrosine was isolated from a crop field soil sample and identified as Klebsiella sp. GSK based on morphological, biochemical, and 16S rDNA sequencing. The polymerization of this pigment occurs outside the cell wall, which has a granular structure as melanin ghosts. Chemical characterization of the pigment particles showed then to be acid resistant, alkali soluble, and insoluble in most of the organic solvents and water. The pigment got bleached when subjected to the action of oxidants as well as reductants. This pigment was precipitated with $FeCl_3$, ammoniacal silver nitrate, and potassium ferricynide. The pigment showed high absorbance in the UV region and decreased absorbance when shifted towards the visible region. The melanin pigment was further charecterized by FT-IR and EPR spectroscopies. A key enzyme, 4-hydroxyphenylacetic acid hydroxylase, that catalyzes the formation of melanin pigment by hydroxylation of L-tyrosine was detected in this bacterium. Inhibition studies with specific inhibitors, kojic acid and KCN, proved that melanin is synthesized by the DOPA-melanin pathway.