• Title, Summary, Keyword: pinitol

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Determination of Total Chiro-inositol Content in Selected Natural Materials and Evaluation of the Antihyperglycemic Effect of Pinitol Isolated from Soybean and Carob

  • Kim, Jung-In;Kim, Jae-Cherl;Joo, Hee-Jeong;Jung, Suk-Hee;Kim, Jong-Jin
    • Food Science and Biotechnology
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    • v.14 no.4
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    • pp.441-445
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    • 2005
  • Pinitol and chiro-inositol exert insulin-like effect by mediating post-receptor signaling pathway. Total chiro-inositol concentrations, including pinitol, chiro-inositol, and their derivatives, were determined in 115 natural and food materials to identify economical sources for mass production of pinitol. Carob pod, Bougainvillea, soy whey, and soybean oligosaccharides were rich sources of chiro-inositol. Pinitol was isolated from soy whey and carob pod, considered as economically viable sources, by chromatographic separation using activated carbon. Soy and carob pinitols had same chemical structure as that of reference pinitol based on HPLC and NMR results. Oral administration of soy pinitol and carob pinitol (10 mg/kg) significantly decreased blood glucose at 2-6 hr in streptozotocin-induced diabetic rats. These results suggest pinitol isolated from soy whey and carob pod could be beneficial in controlling blood glucose in animal model of diabetes mellitus.

Characteristics of Pinitol as a Functional Food Biomaterial (건강기능성 식품소재로서 pinitol의 특성)

  • Son, Min-Sik;Seo, Myung-Seon;Lee, Sang-Han
    • Journal of Life Science
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    • v.19 no.1
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    • pp.147-151
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    • 2009
  • D-pinitol, another chemical structure of 3-O-methyl-D-chiro-inositol, is an important insulin-sensitizer. The purpose of this review is to examine the characteristics of pinitol and other analogs as functional food biomaterials which were well known to reduce blood glucose levels. Pinitol can be converted to chiro-inositol in normal humans, while diabetic patients can not use the molecule, resulting in exhibiting low level of chiro-inositol in their urine. Recently, it is reported that pinitol can trigger phospholipase C/D, thus the rate of glucose metabolism accelerates to use as fuel for human body. To not only reduce insulin resistance of diabetic patients, but also alleviate the symptoms of diabetes, obesity, and muscle contraction, pinitol and its dietary supplementation is needed.

Variation of Pinitol Content for Domestic Legume Species in Korea (국내 수집 두과식물의 부위별 pinitol 함량 변이)

  • Seo, Seung-Min;Jeong, Yeon-Shin;Hari, Dhakal Krisna;Shin, Dong-Hyun;Lee, In-Jung;Park, Eun-Sook;Lee, Jeong-Dong;Hwang, Young-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.1
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    • pp.50-56
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    • 2011
  • This study was designed to investigate variation of pinitol content in different parts of seventeen legume species including silk tree (Albizia julibrissin). D-pinitol has been demonstrated to exert insulin-like and anti-inflammatory effects. These legumes were collected from Gyeongsangbuk-Do in Korea. Significant difference in pinitol content was observed among 17 different legume species. However, it was the highest in sericea lespedeza (Lespedeza cuneata). The highest pinitol content was observed in leaf followed by stem, pod shell, seed and root among plant parts. Legume plants which had higher pinitol content in leaves were chinese pea shrub, bastard indigo, wild cowpea and sericea lespedeza, having 59.9 mg/g, 62.2 mg/g, 69.6 mg/g and 65.4 mg/g, respectively. Stem of astragali radix showed the highest pinitol content among all leagumes, which was 34.0 mg/g. In case of root, kudzuvine showed the highest pinitol content followed by licorice and chinese pea shrub, which were 24.6 mg/g, 16.9 mg/g, and 16.5 mg/g, respectively. However, in the case of pod shell and seed, only lablab purpureus and sericea lespedeza showed the highest pinitol content 52.5 mg/g (for pod shell) and 24.9 mg/g (for seed), respectively. Concluding, the sericea lespedeza showed the highest total pinitol content of whole plant followed by chinese redbud, chinese pea shrub, bastard indigo and silk tree. Considering the pinitol content and harvesting yield, sericea lespedeza can be used as a practical medicinal herb.

Effects of Pinitol Supplementation and Strength Training on Anaerobic Performance and Status of Energy Substrates in Healthy Young Men

  • Lee, Dae-Taek;Lee, Woon-Yong
    • Nutritional Sciences
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    • v.8 no.3
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    • pp.189-195
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    • 2005
  • To assess the effect of pinitol supplementation and strength training for two weeks on the anaerobic capacity during and after exercise, and improvement of glucose metabolism during the recovery period of muscular fatigue with repeated acute bouts of cycling exercise, a total of 24 healthy young men were recruited and randomly and equally divided into three groups; pinitol supplementation group (PSG), placebo group (PLG), and control group (CON). Using a randomized double-blinded design, subjects in PSG were provided pinitol supplement, consumed orally 1.2 g/day, and participated in the resistance exercise program and cycling exercise for two weeks. Subjects in PLG underwent the same protocol as those in PSG but consumed the same amount of placebo. No supplementation and exercise program was given to CON. Before and after the intervention, all subjects were tested for their anaerobic capacities evaluated by Wingate test twice separated by 30 min. During the test, peak anaerobic power (PP), mean anaerobic power, total work, and fatigue index were evaluated During resting and recovery, blood samples were drawn and plasma pinitol, myo-inositol, chiro-inositol, insulin, free fatty acid, glucose, and lactate levels were analyzed After two weeks, PP and relative PP of the second biking were improved from the first biking in PSG only (p<0.05). No changes were found in all other variables of Wingate test in all groups. No statistical differences between groups and pre- and post-intervention were observed in concentrations of pinitol, myo-inositol, and chiro-inositol, but pinitol concentration was higher during recovery compared to the baseline in all groups and testings (p<0.05). Lactate level during recovery was higher than the resting level, but no other blood parameters were significantly changed. In conclusion, two weeks of pinitol supplementation in conjunction with short duration of anaerobic training in healthy young men did not induce any obvious benefits in terms of anaerobic capacity and energy metabolism Individual and/or population susceptibility may be one factor responsible for adopting pinitol supplementation.

Inhibitory Effect of D-pinitol on Both Growth and Recurrence of Breast Tumor from MDA-MB-231 Cancer Cells (D-Pinitol의 유방암 증식 및 재발 억제 효능)

  • Kim, Yoon-Seob;Park, Ji-Sung;Kim, Minji;Hwang, Bang Yeon;Lee, Chong-Kil;Song, Sukgil
    • Korean Journal of Pharmacognosy
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    • v.45 no.2
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    • pp.174-180
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    • 2014
  • D-Pinitol, an anti-diabetic substance, is a naturally occurring compound found in legumes. In this study, we investigated the inhibitory effect of D-pinitol on growth and recurrence of breast cancer. When D-pinitol was treated on MDA-MB-231 or MCF-7 breast cancer cells, it was observed that the viability of the two cancer cell lines was reduced in MTT assay. In order to examine the effect on the growth of breast tumor, mouse xenograft assay was carried out. On day 0, nine millions cells of MDA-MB-231 were injected subcutaneously into nude mouse and D-pinitol was administered orally at the dose of 500 mg/kg or 1000 mg/kg body weight for consecutive 45 days. Tumor size was reduced in dose-dependent manner upto 95.4% in 1000 mpk-treated group, compared with the non-treated control group. When D-pinitol was co-administrated with $4{\mu}g$ of doxorubicin, recurrence of breast tumor was delayed by two weeks, compared with the mouse group of doxorubicin monotherapy. Consistent with this data, it was observed that the population of cancer stem cells (CSCs), responsible for recurrence of cancer, within tumor mass was significantly reduced. Taken together, D-pinitol inhibits the growth of breast cancer and relapse of the tumor by suppressing the proliferation of CSCs.

Comparison of Immuno-Suppressive Activities of Pinitol Isolated from Soybean (콩으로부터 추출한 Pinitol의 면역억제 활성 비교)

  • Park, Chul-Hong;Heo, Jin-Chul;Nam, Dong-Yun;Lee, Si-Rim;Nam, So-Hyun;Son, Min-Sik;Hwang, Young-Hyun;Lee, Sang-Han
    • Korean Journal of Food Preservation
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    • v.16 no.3
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    • pp.449-453
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    • 2009
  • The experiment was conducted to validate anti-inflammatory effects of pinitol from bean. It was evaluated for some molecule targets by wound healing assay and RT-PCR. The results of wound healing assay was shown dose-dependent inhibition of cell migration in cancer cells and inhibited RNA expression of ICAM-1, CD 44, MMP-17, MMP-14 and ARF2. Immune suppression activity in a mouse provoked by DNFB observed that inflammatory reaction with pinitol were reduced ear swelling and inflammatory cells infiltration in mouse atopic models. The result confirmed that pinitol have the effect of dose-dependent immune suppression activity.

Isolation of Gene according to the Physiological Changes of Lespedeza cuneata. G don by the Convergence Study using a Computer Program and NGS (Next Generation Sequencing) (NGS (Next Generation Sequencing)와 컴퓨터 프로그램의 융합적 연구를 통한 비수리(Lespedeza cuneata. G. don)의 생리적 변화에 따른 유용 유전자 분리)

  • Ahn, Chul-Hyun
    • Journal of the Korea Convergence Society
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    • v.8 no.12
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    • pp.31-38
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    • 2017
  • This study was carried out to investigate the possibility of isolating the useful gene of soybean plant, anthocyanin, through NGS (Next Generation Sequencing) and molecular biology experiments. Lespedeza cuneata. G. don is a resource plant but has many useful materials. Especially, D-pinitol, which has anti-diabetic function, is contained in a large amount. However, the gene related to the biosynthesis of D-piniol has not been isolated in the non-spermatid. Lespedeza cuneata. G. don was treated with abiotic stress (drought), total RNA was extracted, and a library was constructed to perform NGS. In this way, the genes involved in D-pinitol biosynthesis were isolated and sequenced in silico. In order to support this, ononitol epimerase involved in D-pinitol amplification was identified using the Blast program and RT-PCR confirmed the increased gene expression in vitro, and the gene was isolated and identified by convergence study.

D-Pinitol Promotes Apoptosis in MCF-7 Cells via Induction of p53 and Bax and Inhibition of Bcl-2 and NF-κB

  • Rengarajan, Thamaraiselvan;Nandakumar, Natarajan;Rajendran, Peramaiyan;Haribabu, Lingaiah;Nishigaki, Ikuo;Balasubramanian, Maruthaiveeran Periyasamy
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.4
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    • pp.1757-1762
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    • 2014
  • Development of drugs from natural products has been undergoing a gradual evoluation. Many plant derived compounds have excellent therapeutic potential against various human ailments. They are important sources especially for anticancer agents. A number of promising new agents are in clinical development based on their selective molecular targets in the field of oncology. D-pinitol is a naturally occurring compound derived from soy which has significant pharmacological activitites. Therefore we selected D-pinitol in order to evaluate apoptotic potential in the MCF-7 cell line. Human breast cancer cells were treated with different concentrations of D-pinitol and cytotoxicity was measured by MTT and LDH assays. The mechanism of apoptosis was studied with reference to expression of p53, Bcl-2, Bax and NF-kB proteins. The results revealed that D-pinitol significantly inhibited the proliferation of MCF-7 cells in a concentration-dependent manner, while upregulating the expression of p53, Bax and down regulating Bcl-2 and NF-kB. Thus the results obtained in this study clearly vindicated that D-pinitol induces apotosis in MCF-7 cells through regulation of proteins of pro- and anti-apoptotic cascades.

Increase of the D-chiro-inositol and D-Pinitol Contents by Abiotic Stress in the Buckwheat Seedlings (쓴메밀 유식물에서 비 생물학적 스트레스에 의한 D-chiro-inositol과 D-pinitol의 함량 증가)

  • Ahn, Chul-Hyun;Park, Phun-Bum
    • Journal of Life Science
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    • v.19 no.10
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    • pp.1456-1462
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    • 2009
  • D-chiro-Inositol, which is the isomer of myo-inositol, is a well known drug for the treatment of type II diabetes. The methylated form of D-chiro-inositol, D-pinitol and D-chiro-inositol are synthesized when the plants are exposed to the abiotic stresses such as drought, salinity and low temperature as osmoprotectants. In soybean, myo-inositol is converted to ononitol by O-methyltransferase, and ononitol is converted to D-pinitol by ononitol epimerase and finally converted to D-chiro-inositol by demethylase. However there have been some reports that in buckwheat, myo-inositol can be converted to D-chiro-inositol directly. This study was conducted to determine the changes of soluble cyclitols in buckwheat seedlings after exposure to salt and drought stresses by GC-FID. The results indicated that myo-inositol may be the precursor of D-chiro-inositol biosynthesis.

Comparison of NaCl and D-Pinitol Content of Freeze-Dried Ice Plant, Natural and Purified Commercial Salts and their Radical Scavenging Activity

  • Sim, Wan-Sup;Park, Sung-Ho;Choi, Sun-Il;Cho, Bong-Yeon;Choi, Seung-Hyun;Han, Xionggao;Jang, Gill-Woong;Kwon, Hee-Yeon;Choi, Ye-Eun;Men, Xiao;Yoon, Sangjin;Kim, Young-Jun;Cho, Ju-Hyun;Lee, Ok-Hwan
    • Journal of Food Hygiene and Safety
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    • v.34 no.6
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    • pp.595-600
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    • 2019
  • This study investigated the suitability of freeze-dried ice plant (Mesembryanthemum crystallinum) as the source of an edible healthy salt alternative by examining the content of NaCl, D-pinitol, total phenols, total flavonoids, and DPPH radical scavenging activity compared with those of natural and purified salts. Our results showed that the NaCl content of freeze-dried ice plant, natural and purified salts was 19, 94 and 99%, respectively. The D-pinitol content of freeze-dried ice plant was 777 mg/100 g, whereas D-pinitol was not detected in either natural or purified salts. The total phenol and flavonoid contents of freeze-dried ice plant were 115 mg GAE/100 g and 985 mg RE/100 g, respectively. The DPPH radical scavenging activity of freeze-dried ice plant was markedly higher than that of natural and purified salts. Altogether, these results show that freeze-dried ice plant contains both NaCl and bioactive compounds and can be used as a source of edible salt with positive health effects.