• Title, Summary, Keyword: polygodial

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Synergistic Effect of Polygodial with Imidazole Drugs on the Antifungal Activity (천연물 Polygodial과 Imidazole계 화합물의 병용에 의한 항진균 활성의 증진)

  • 이상화;이재란;김창진
    • YAKHAK HOEJI
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    • v.43 no.2
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    • pp.221-227
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    • 1999
  • The fungistatic and fungicidal activities of amphotericin B, fluconazole, miconazole, econazole, and 5-fluorocytosine against Saccharomyces cerevisiae were estimated in the presence of 1/2 minimum inhibitory concentration (MIC) and 1/2 minimum fungicidal concentration (MFC) of polygodial, respectively. Among them, the antifungal activities of miconazoles by polygodial was still shown against several yeast-like fungi including Candida albicans, Candida utilis, Cryptococcus neoformans, except for Candida krusei. The combination of polygodial with imidazole drugs against Saccharomyces cerevisiae was further examined using the macrobroth dilution checkerboard method. The fractional inhibitory concentration (FIC) and the fractional fungicidal concentration (FFC) index between polygodial and miconazole were 0.16 and 0.19, while the combination of polygodial with econazole exhibited the FIC index of 0.19 and the FFC of 0.25, respectively. These results suggest that polygodial and the imidazoles on the fungistatic and fungicidal action are highly synergistic.

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Antifungal Activity of Polygodial against Food-Contaminants and Effects of Temperature and pH on the Action (Polygodial의 식품 오염 진균에 대한 항균 활성과 그 활성능에 미치는 온도 및 pH의 영향)

  • 이재란;이상화;홍순덕
    • Journal of Life Science
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    • v.8 no.4
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    • pp.421-425
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    • 1998
  • The antifungal activity of polygodial, which was isolated from Polygonum hydropiper used as a hot spice in Japan, was investigated through measuring its MICs and against food-contaminants including 4 strains of Saccharomyces cerevisiae, 3 strains of Zygosaccharomyces species, and 5 strains of Aspergillus species. All yeast-like fungi exhibited strong antifungal susceptivilities with MICs and MFCs below 3.13${\mu}g$/ml. Strains of Aspergillu species showed moderate with MIC of 25-50${\mu}g$/ml and MFC of 50-100${\mu}g$/ml. However these strains to sorbic acid, as already known, revealed very weak with MICs of 200-800${\mu}g$/ml and MFCs above 1600${\mu}g$/ml. In addition, effects of various pHs and temperatures on the antifungal activity of polygodial were tested against S. cerevisiae. At $4^{\circ}C$, the fungicidal activity of polygodial gradually increased with incubation time and reached the maximum(MFC of 3. 13${\mu}g$/ml) in 5 hours. At temperatures in the range of 30-$45^{\circ}C$, the activity of polygodial increased in proportion to temperature rise and in particular at $45^{\circ}C$ was possible with the concentration of 0.1${\mu}g$/ml. mOn the other hand, medium pH was also identified to affect the antifungal activity of polygodial. Namely, compared to neutral (pH7), acidic(pH 3) and basic(pH 9) medium synergized its activity (MIC and MFC) to 8- and 2- fold, respectively.

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Antifungal Activity of EDTA and Combinatory Synergism of EDTA with Polygodial against Saccharomyces cerevisiae (Saccharomyces cerevisiae에 대한 EDTA의 항진균 활성 및 EDTA와 Polygodial 간의 병용 효과)

  • Lee, Sang-Hwa;Lee, Jae-Ran;Kim, Chang-Jin
    • Korean Journal of Food Science and Technology
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    • v.31 no.4
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    • pp.1109-1114
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    • 1999
  • The antifungal activity of ethylenediaminetetraacetic acid (EDTA), an antioxidative food preservative, was investigated against Saccharomyces cerevisiae. At the general condition for antifugal test, EDTA exhibited the minimum inhibitory concentration (MIC) of 200 ${\mu}g/ml$ and the minimum fungicidal concentration (MFC) of 6,400 ${\mu}g/mL$, As seen with the antibacterial activity, the antifungal activity of EDTA was greatly decreased by high inoculum size, acidic medium, and cation ($Ca^{++},\;or\;Mg^{++}$) added to medium. On the other hand, when EDTA was combined with polygodial isolated from natural food spice, they showed strong synergism on the antifungal activity. Further, the diminishing antifungal activity of EDTA by high inoculum size, acidic medium, and cation ($Ca^{++}\;or\;Mg^{++}$) added to medium was considerably improved by the combination with polygodial.

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