• Title, Summary, Keyword: porcine epidemic diarrhea virus

Search Result 55, Processing Time 0.039 seconds

Serological evidence on the persistence of porcine epidemic diarrhea virus infection (돼지 유행성 설사병(porcine epidemic diarrhea)의 상재화에 대한 혈청학적 증명)

  • Park, Bong-kyun;Han, Kyung-soo;Lyoo, Kwang-soo;Kim, Jun-young;Jeong, Hyun-kyu
    • Korean Journal of Veterinary Research
    • /
    • v.38 no.4
    • /
    • pp.818-822
    • /
    • 1998
  • The persistence of porcine epidemic diarrhea virus(PEDV) infection was demonstrated in 7 swine farms employing continuous pig flow management even after seasonal outbreaks. Clinically, sporadic postweaning diarrhea was a major concern in those farms. Subsequently circulatory antibody detection using serum neutralizing test made useful for confirmation of PEDV persistent infections. The persistence of PEDV in the premise might have induced recurrence over the period of time.

  • PDF

Biological and Physicochemical Properties of Porcine Epidemic Diarrhea Virus Chinju99 Strain Isolated in Korea (국내 분리 돼지 유행성설사 바이러스 Chinju99주의 생물학적 및 물리화학적 성상)

  • Lee, Hee-Kyung;Yeo, Sang-Geon
    • Journal of Veterinary Clinics
    • /
    • v.20 no.2
    • /
    • pp.150-154
    • /
    • 2003
  • A disease with severe diarrhea occurred in a herd of one thousand, 1-week-old piglets in Chinju, Korea, and was diagnosed as porcine epidemic diarrhea by the detection of N gene of porcine epidemic diarrhea virus (PEDV) from small intestines. A PEDV, named as Chinju99, was also isolated from the intestines after two blind-passages in Vero cells supplemented with trypsin (10 ug/ml). and the biological and physicochemical properties of the isolate were characterized. The virion was roughly spherical in shape and had spike peplomers on its outer surface. The virus exhibited cytopathic effects such as rounding degeneration at initiation of infection and syncytia formation later in Vero cells. The virus was labile to 20% ether and 5% chloroform but stable in acid with pH 4-7 at $4^{\circ}C$. The infectivity of the virus was maintained at $50^{\circ}C$ for 180 min, and the buoyant density of the virus in sucrose was 1.180 g/ml. All biological and physicochemical properties of the virus were typical features of coronaviruses.

Rapid and Easy Detection of Porcine Epidemic Diarrhea Virus (PEDV) by in situ Hybridization (In Situ Hybridization에 의한 돼지 유행성설사증 (Porcine Epidemic Diarrhea)의 진단)

  • Park, Nam-yong;Cho, Ho-seong;Kim, Tae-ju;Park, Young-seok
    • Korean Journal of Veterinary Research
    • /
    • v.43 no.3
    • /
    • pp.477-483
    • /
    • 2003
  • Molecular diagnostic techniques have been used to identify porcine epidemic diarrhea virus (PEDV), a causative agent of acute enteritis in swine, but they were difficult to be petformed and time-consuming. To detect PEDV in a rapid and easy way, we developed biotinylated cDNA probe for N gene encoding the nucleoproteins of PEDV. Formalin-fixed and paraffin-embedded tissues from 24 naturally infected pigs were used for the experiment. The ISH produced a positive reaction in all cases. When intestinal tissues were hybridized with PEDV probe, strong signals were seen in the villus enterocytes of the jejunum and ileum. Hybridization signals were also found in the duodenum from one pig and in colon from dnother. In conclusion, ISH with a biotinylated cDNA probe was provided to be a useful diagnostic method for detecting PEDV effectively in routinely processed tissue sections.

Virucidal efficacy of a disinfectant solution composed of n-alkyl-dimethyl-benzyl-ammonium chloride against porcine epidemic diarrhea virus

  • Cha, Chun-Nam;Yu, Eun-Ah;Yoo, Chang-Yeul;Cho, Ki-Yung;Lee, Soo-Ung;Kim, Suk;Lee, Hu-Jang
    • Korean Journal of Veterinary Service
    • /
    • v.37 no.2
    • /
    • pp.105-110
    • /
    • 2014
  • Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED) and causes a considerable economic loss in swine industry. In this study, the virucidal efficacy of the disinfectant composed to n-alkyl-dimethyl-benzyl-ammonium chloride (n-ADBAC) was investigated against PEDV. A virucidal efficacy was determined with the viability of PEDV contacted with the disinfectant in Vero cells. The disinfectant and PEDV were reacted on the hard water (HW) or organic matter suspension (OM) condition. On HW condition, PEDV was inactivated with 50 fold dilutions of the disinfectant. When the antiviral effect on OM condition was evaluated, the antiviral activity of the disinfectant showed on 10 fold dilutions against PEDV. As the disinfectant possesses the virucidal efficacy against PEDV, the disinfectant solution can be used to limit the spread of animal viral diseases.

Reemergence of porcine epidemic diarrhea virus on Jeju Island

  • Lee, Sunhee;Ko, Deok-Ho;Kwak, Seong-Kyu;Lim, Chung-Hun;Moon, Sung-Up;Lee, Du Sik;Lee, Changhee
    • Korean Journal of Veterinary Research
    • /
    • v.54 no.3
    • /
    • pp.185-188
    • /
    • 2014
  • Porcine epidemic diarrhea virus (PEDV) strains responsible for recent outbreaks in the United States have been occurring in Mainland Korea since late 2013. Over the past 10 years, PEDV outbreaks have not been reported on Jeju Island. However, in late March of 2014, PEDV re-emerged on Jeju Island and was found to be genetically identical to PEDV strains currently circulating in Mainland Korea. The present study was conducted to provide a better understanding of the epidemiology of PEDV and more effective preventive measures against PED.

Pathological findings of experimentally infected piglets with a porcine epidemic diarrhea virus isolated in Korea (국내에서 분리된 돼지 유행성 설사증 바이러스 접종자돈의 병리학적 소견)

  • Kim, Gye-Yeop;Park, Nam-Yong;Chung, Chi-Young;Park, Young-Seok
    • Korean Journal of Veterinary Pathology
    • /
    • v.2 no.2
    • /
    • pp.107-116
    • /
    • 1998
  • Twelve colostrum-deprived piglets were inoculated oral1y with porcine epidemic diarrhea virus isolated from Korea. The piglets were euthanatized from at 12 hour postinoculation(PI) at 6-hour intervals. At 24 to 36 hours PI, all infected piglets showed severe yellowish to watery diarrhea, dehydration, depression, and anorexia. At necropsy, the intestinal wall looked thin, mesenteric vessels congested, and mesenteric lymph nodes edematous. The histological findings showed marked villous atrophy and fusion, severe degeneration of enterocytes and monomuclear cell infiltration in the lamina propria. On electron microscopy, villous epithelial cells of infected jejunum contained viral particles at 18 hour PI. The viral particles were pleomorphic spheres with a mean diameter of 95∼180nm including 18nm projections.

  • PDF

Isolation of porcine epidemic diarrhea virus (PEDV) in Korea (돼지 유행성 설사 바이러스 (porcine epidemic diarrhea virus)의 국내 분리주 작성에 관한 연구)

  • Kweon, Chang-hee;Kwon, Byung-joon;Jung, Tae-sung;Kee, Young-jin;Hur, Dong-ho;Hwang, Eui-kyung;Rhee, Jae-chin;An, Soo-hwan
    • Korean Journal of Veterinary Research
    • /
    • v.33 no.2
    • /
    • pp.249-254
    • /
    • 1993
  • The etiological survey on porcine epidemic diarrhea virus(PEDV) by immunofluorescence antibody test(IFA) showed the positive rusult from the intestines of piglet died from acute diarrhea. The viral agent of PED was also isolated from intestine, which showed positive reaction by immunofluorescence test. After passage in Vero cell, the viral agent was further cloned by plaque purification and designated as KPEDV-9. The immunoblotting analysis using hyperimmune sera and porcine sera revealed the presence of several polypetide bands with molecular weight(M.W.) of 88K, 74K, 70K, 58~54, 54~46K, 44~40K and 33~32K, respectively.

  • PDF

Ultrastructural Changes in Enterocyte infected with Porcine Epidemic Diarrhea Virus (돼지 유행성 설사증 (Porcine Epidemic Diarrhea) 감염 소장상피세포의 전자현미경 관찰)

  • Park, Nam-Yong;Koo, Kyoung-bon;Kim, Seung-Jae;Chung, Chi-Young;Park, Young-Seok;Cho, Kyoung-Oh;Kim, Yong-Hwan;Im, Hyung-Ho
    • Korean Journal of Veterinary Pathology
    • /
    • v.3 no.1
    • /
    • pp.35-44
    • /
    • 1999
  • The morphologic changes of small intestinal epithelium in pigs diagnosed as porcine epidemic diarrhea(PED} by virus isolation and immunohistochemistry were studied through light microscope and transmissible electron microscope. On semi-thin section, the histologic findings showed severe villous atrophy and fusion with hyperplasia of cuboidal epithelium in the villi, inflammatory cell infiltration in lamina propria, and increased mitotic figures in the crypt. The structural changes were mostly restricted to the cytoplasm of affected absorptive epithelium of villi. 3 types of epithelial changes were found; degenerated virus-affected cells, undifferentiated cuboidal cells, and normal columnar cells. On electron microscopy, round to spherical viral particles of 50∼l00nm in diameter were found within the dilated vesicles and endoplasmic reticulums of degenerated cells, which had decreased their cytoplasmic electron density due to dilated and missing organelles(e.g. mitochondria, ERs, etc.). Microvilli were shortened and sparse, leaving denuded terminal web of the villous epithelial cells. Fat globules were often found within slightly degenerated enterocytes. On the tip of villi, severely damaged cells were exfoliated and replaced by undifferentiated cuboidal cells We found distinct ultrastructural changes in the jejunal epithelium confirming PED virus infection is involved in malabsorptive diarrhea.

  • PDF

Isolation and Identification of Porcine Epidemic Diarrhea Virus (돼지 유행성 설사 바이러스의 분리동정)

  • Park Nam-young;Cho Kyung-oh;Cho Seoung-su;Ha Yong-kong
    • Journal of the korean veterinary medical association
    • /
    • v.29 no.6
    • /
    • pp.360-365
    • /
    • 1993
  • Attempts to isolate porcine epidemic diarrhea virus(PEDV) were made by inoculating feces homogenates into Vero cells cultures with trypsin. Cytopathic effects(CPE), such as vacuolation, formation of syncytia, and fusion of cells, were detected even at fir

  • PDF

Immunohistochemical study of causative agents for the porcine viral diarrhea disease on newborn piglets in Cheju (제주지역 양돈장에서 발생한 자돈 설사형 바이러스의 면역조직화학적 확인)

  • 강완철;김진회;안미정;강종철
    • Korean Journal of Veterinary Service
    • /
    • v.23 no.3
    • /
    • pp.301-306
    • /
    • 2000
  • In an outbreak of acute porcine diarrhea in newborn piglets, an etiological study was carried out using piglets submitted in Cheju Province Institute for Livestock Promotion(Cheju Veterinary Service for the disease diagnosis). Sixteen piglets(2-7 days old) were collected from 4 farms during outbreaks of diarrhea disease(from January to April 2000). Specimens were taken after necropsy and examined by immunohistochemistry using of monoclonal antibodies for porcine epidemic diarrhea(PED) virus, transmissible gastroenteritis(TGE) virus, and porcine rotavirus. Immunohistochemistry showed that PED virus antigens, but both TGE virus and rota virus antigens not, were localized in the some epithelial cells of the intestines of 14 animals among 16 piglets examined. PEB virus antigens were mainly detected in the cytoplasm of enterocytes. Infected cells, which were most abundant in the villous epithelial cells of the jejunum and ileum, were uncommon in the crypt, epithelial cells, the lamina propria and Peyer's patches of piglets examined. The results suggest that PED virus is one of the most prevailing agents in an outbreak of fatal diarrhea in newborn piglets on Cheju island and PED virus was need to further study to prevent this disease.

  • PDF