• Title, Summary, Keyword: postweaning multisystemic wasting syndrome

Search Result 17, Processing Time 0.03 seconds

Detection of viral pathogens and isolation of porcine circovirus 2 from postweaning multisystemic wasting syndrome-affected piglets (이유자돈 전신소모성증후군 이환 자돈에서의 바이러스성 원인체 검색 및 porcine circovirus 2 분리동정)

  • Park, Choi-Kyu;Kim, Hyun-Soo
    • Korean Journal of Veterinary Research
    • /
    • v.44 no.4
    • /
    • pp.561-569
    • /
    • 2004
  • To detect viral agents and isolate porcine circovirus 2 (PCV2), 60 samples of lung and lymph node were collected from 5 to 12 week-old pigs that had showed clinical signs of postweaning multisystemic wasting syndrome (PMWS). Polymerase chain reactions (PCRs) were conducted to identify the viral pathogens including PCV1, PCV2, porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV) that have been considered to be the causal agents of PMWS. Among 60 samples, PCV 2 was detected from 57 samples but no PCV 1 was detected. PRRSV and/or PPV were also detected from 27 (47.4%) samples and 1 (1.8%) sample of these 57 PCV 2-positive samples, respectively. Tissue homogenates were inoculated onto PCV-free PK-15 cell monolayers. Seven isolates were confirmed as PCV 2 by multiplex PCR, indirect immunofluorescence assay, and transmissible electron microscopy. These date suggest that PRRSV is a major cofactors causing PMWS in pigs that were infected with PCV2 in Korea.

Porcine Circovirus Infection in Weaned Pigs with Postweaning Multisystemic Wasting Syndrome in Korea (국내 이유자돈의 써코바이러스 감염에 의한 이유후전신소모성 증후군)

  • Kim, Jae-hoon;Roh, In-soon;Sohn, Hyun-joo;Jean, Young-hwa;Hwang, Eui-kyung;Yoon, Kyoung-jin
    • Korean Journal of Veterinary Research
    • /
    • v.43 no.3
    • /
    • pp.463-469
    • /
    • 2003
  • Eight nursery to grower pigs exhibiting weight loss and sudden death were diagnosed as postweaning multisystemic wasting syndrome (PMWS) based on the results of gross findings, histopathology, immunohistochemistry, fluorescent antibody test, virus isolation, PCR, serology, and electron microscopy. Groosly, the pigs had a rough hair coats and were severely emaciated. And moot lymph nodes were pale and enlarged. Lungs were not fully collapsed and exhibited 10 to 40% pale red cranioventral consolidation. Histopathologically, typical lymphohistiocytic interstitial to bronchointerstitial pneumonia, chronic lymphadenitis, severe lymphoid depletion, and basophilic intracytoplasmic inclusions were noted in the most lymphoid tissues. Porcine circovirus panicles were observed in the inguinal lymph node of the pigs by electron microscopy. Porcine circovirus type 2 (PCV2) antigens or viral DNAs were detected in the lesions of all pigs using immunohistochemistry or PCR. Two PCV2 were isolated from a homogenate of pooled lung and lymph node in 2 of the 5 pigs. Additionally, antigens of porcine reproductive and respiratory syndrome virus (PRRSV) and Hemophilus (H.) parasuis were also detected by immunofluorescent antibody test. Serologically, 55% of randomly selected sows and fattening pigs was serum antibody positive to PCV2 by an indirect fluorescent antibody (IFA) test and approximately 18 % of animals in the herd were serologically pooitive by the ELISA kit for PRRSV. To our knowledge, this is the first report of PMWS co-infected with PCV-2, PRRS, and H. parasuis in Korea.

Postweaning Multisystemic Wasting Syndrome - Case studies

  • Kim, Jae-Hoon;Roh, In-Soon;Hwang, Eui-Kyung;Woo, Gyu-Hywong;Jean, Young-Hwa;Sohn, Hyun-Joo;Yoon, Kyoung-Jin;Janke, Bruce H.;Park, Choi-Kyu;Kim, Ki-Seuk
    • Proceedings of the Korean Society of Veterinary Pathology Conference
    • /
    • /
    • pp.28-28
    • /
    • 2000
  • Postweaning Multisystemic Wasting Syndrome (PMWS) is a recently described disease of growing pigs characterized by progressive weight loss and chronic pneumonia. PMWS was first identified in western Canada in 1995, and has now been diagnosed in the US, Europe and Asia. Since 1999, 57 pigs from 23 farms of PMWS have been diagnosed in the National Veterinary Research and Quarantine Service (NVRQS). The most common age of onest was clustered around 6-week age. (omitted)

  • PDF

Prevalence of porcine circovirus type 2 from slaughtered pigs in eastern area of Gangwon province (강원도 영동지역의 도축돈에 대한 porcine circovirus type 2 감염율 조사)

  • Choi Won-Zong;Hong Gyong-Soo;Jeong Woong-Ho;Kim Nam-Sun;Kim Nyeun-Su;Kim Ki-Tae;Kim Kwang-Jae;Kim Moon-Shik
    • Korean Journal of Veterinary Service
    • /
    • v.29 no.3
    • /
    • pp.249-256
    • /
    • 2006
  • Porcine circovius type 2 (PCV 2) is a novel virus of Circoviridae familiy which is considering the cause of postweaning multisystemic wasting syndrome (PMWS). This study was carried out to investigate the prevalence of PCV 2 infection of swine in eastern areas of Gangwon province from February to June in 2005. Polymerase chain reaction (PCR) were conducted to identify the PCV 2 genome against 80 pigs. The number of infection and its rate of 4 areas, 8 farms and 80 pigs were 3 (75.0%), 7 (88.0%) and 44 (55.0%), respectively.

Occurrence of postweaning multisystemic wasting syndrome in pigs in Jeju (제주지역 양돈장 자돈에서 발생한 이유후 전신성 소모성 증후군의 증례)

  • Kang, Jong-chul;Jeong, Kyong-ju;Ahn, Mee-jung;Lee, Du-sik;Kang, Wan-chul;Kim, Jin-hoe;Shin, Tae-kyun
    • Korean Journal of Veterinary Research
    • /
    • v.41 no.3
    • /
    • pp.367-371
    • /
    • 2001
  • Multiplex PCR and immunohistochemistry were used to detect and differentiate between porcine circovirus (PCV) type-I and the PCV associated with postweaning multisystemic wasting syndrome (PMWS). Unique DNA product to PCV type-II was confirmed the some organs including lymph nodes, tonsil and spleen from eight pigs in Jeju by multiplex PCR. In this study, the samples were tested by a multiplex PCR assay to determine the type of PCV in each case; all cases were PCV type-II positive. PCV type-II was identified not only in typical PMWS cases, but also in field cases submitted with various clinical histories, some of which were not suggestive of PMWS. Immunohistochemically PCV type-II antigen was detected in macrophage-like cells in the tonsil, liver, lymph nodes and spleen, while some hepatocytes and renal tubular epithelial cells were also positive to the virus. This study suggested that PCV type-II is one of the causative agents of PMWS as well as the major type of PCV in the affected pigs in Jeju.

  • PDF

Prevalence of porcine parvovirus in pigs with postweaning multisystemic wasting syndrome in Jeju Island

  • Ko, Kyeong-Nam;Jung, Ji-Youl;Kang, Sang-Chul;Kim, Ki-Seung;Kim, Jae-Hoon;Kim, Dae-Yong;Hwang, Eui-Kyung;Kim, Jae-Hoon
    • Korean Journal of Veterinary Research
    • /
    • v.51 no.3
    • /
    • pp.203-208
    • /
    • 2011
  • Postweaning multisystemic wasting syndrome (PMWS), which was first identified in western Canada in 1991 and more recently in the United States, Europe and Asia, is an emerging disease in pigs. Porcine circovirus type 2 (PCV-2) is the primary infectious viral agent causing PMWS, but the full expression of the disease may require the presence of other agents. It is reported that there is apparent synergism between PCV-2 and porcine parvovirus (PPV) in increasing the severity of the clinical signs and lesions of PMWS. From January 2006 to May 2008, a total of the 154 lymph node samples were collected from 4~12 weeks old pigs which had been submitted to the College of Veterinary Medicine, Jeju National University, Korea. These pigs were diagnosed as PMWS on the basis of clinical and pathological examination from 48 commercial herds in Jeju Island. Based on the immunohistochemistry, porcine parvovirus was detected in 69 cases (44.8%) from 154 weaned or grower pigs. PPV antigens were detected in the cytoplasm of histiocytic cells multifocally infiltrated in the cortex and paracortex of lymph nodes. The results of this study clarify that PPV is prevalent in pigs with PMWS on Jeju Island. Therefore PPV is one of the most important co-agents in the development of naturally acquired PMWS. This study may be helpful to the control of this disease and to epidemiological aspects.

Aerodynamic Approaches for Estimation of Waste Disease Spread in Pig Farm through Airborne Contaminants (양돈장의 소모성질병 확산 분석을 위한 현장 모니터링 및 공기유동학적 분석)

  • Seo, Il-Hwan;Lee, In-Bok;Moon, Oun-Kyung;Kwon, Kyeong-Seok
    • Journal of The Korean Society of Agricultural Engineers
    • /
    • v.56 no.1
    • /
    • pp.41-49
    • /
    • 2014
  • Pig chronic wasting disease, including porcine reproductive and respiratory syndrome (PRRS) and postweaning multisystemic wasting syndrome (PMWS), have made a continuous economic damage in pig farms. Airborne spread of livestock viruses are an important spread factor which is difficult to analyze due to invisible airflow and limitation of measurement. The objective of this study is to analyze airborne disease spread between buildings in the experimental pig farm by means of field experiment and computational fluid dynamics (CFD). The field experiments were conducted to capture airborne virus using air sampler and teflon filter along multi points in the experimental pig farm. The samples were tested in terms of virus detection resulting in positive reaction for PRRS and PCV-2 viruses, which can be a firm evidence of airborne virus spread. The CFD simulation model was developed by considering complex topography, wind conditions, building arrangement, and ventilation systems and was used to analyze airborne virus spread according to different wind conditions. The CFD computed result showed a possibility of airborne virus spread via livestock aerosol from infected pig house to neighboring pig houses according to wind directions. The CFD simulation technique is expected to provide significant data for estimating and making a counterplan against airborne disease spread.