• Title, Summary, Keyword: production activity

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Effects of Bacterial Lipopolysaccharide on Prostaglandin Production in Primary Cultured Rat Vascular Smooth Muscle Cells (일차 배양 랫드 혈관 평활근 세포에서 Prostaglandin 생성에 미치는 Bacterial Lipopolysaccharide의 작용 특성)

  • 이수환
    • Journal of Food Hygiene and Safety
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    • v.11 no.4
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    • pp.227-234
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    • 1996
  • This study was designed to characterize endotoxin-induced prostaglandin production in primary cultured rat vascular smooth muscle cells (VSMC). The time course for prostaglandin synthesis in lipopolysaccharide (LPS)-stimulated VSMC showed that the maximum production was reached in 12 hours. LPS induced prostaglandin H2 synthase (PGHS) activity in VSMC and the time course profile in the changes of PGHS activity paralleled that of total prostaglandin production. Differential treatment showed that 4 hours' exposure to LPS was enough for the maximum effect on the prostaglandin production and this effect was completely inhibited by the co-treatment of actinomycin D, a transcription inhibitor. These results suggest that LPS effect might be determined within 4 hours. Actinomycin D increased PGHS activity without affecting prostaglandin production if added 4 hours after LPS treatment. On the other hand, cyclogeximide, a translation inhibitor, augmented LPS-induced prostaglandin production if treated during first four hours, but it inhibited LPS-induced PGHS activity regardless of treatment schedule. These results suggest the existence of multiple regulating mechanisms in the LPS-induced prostaglandin synthesis.

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Activity-oriented Modeling of Mass Production System (대량생산 체제의 Simulation을 위한 Activity 중심 Modeling)

  • Choe, Byeong-Gyu;Park, Seong-Ju;Sin, Ha-Yong
    • Journal of Korean Institute of Industrial Engineers
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    • v.12 no.1
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    • pp.119-131
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    • 1986
  • Described in this paper is a modelling methodology for mass production system simulation. The mass production system under consideration consists of various types of flow lines, special purpose production facilities, conveyor lines, palletized carts, and storage facilities. This type of production system is typical in home appliance industry, automobile industry, footwear industry, etc. where a variety of product mix are mass-produced. The modelling methodology is based on the "discrete-event formalism", and an "activity-oriented world view" is adopted to formalize the system description. A distinctive feature of the modelling methodology is that only the static structure (ie, system components) is included in the fixed model. The dynamic structure of the system is specified through a "data-driven" mechanism, which is an extension of the "experimental frame" concept. Each type of system components (ie, flow line, conveyors, carts, etc.) is formally modeled by using Activity Cycle Diagrams. The issue of "model structuring" is also addressed. The modeling methodology has been successfully applied in a real simulation study of a mass production system.

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An Effectivity Analysis of Production Control Policies Based on Demand and Production Characteristics (수요 및 생산특성에 따른 생산통제 기법간의 효율성 분석에 대한 연구)

  • Lee, Jang-Han;Jeong, Han-Il;Park, Jin-Woo
    • Journal of Korean Institute of Industrial Engineers
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    • v.23 no.2
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    • pp.403-420
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    • 1997
  • In this paper, we examine the effect of production uncertainty to production control policies. First, we examine two famous production control policies, namely, MRP and JIT from the view point of shop floor control perspective, and analyze the differences between them due to demand fluctuations and activity time variations. Second, we conduct simulation studies on MRP and JIT to draw out the effects of demand fluctuations and activity time variations. Demand fluctuations are further classified into demand lumpiness and demand irregularity. And, activity time variations are further classified into stationary time variations and non-stationary time variations. Experimental results show that, in terms of demand fluctuations, MRP is affected by demand lumpiness, but JIT by demand irregularity. And we also see that both MRP and JIT are influenced by stationary time variation with respect to activity time variations.

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Effects of Protein Kinase Inhibitors on Melanin Production in B16 Melanoma Cells Stimulated via Cyclic AMP-dependent Pathway (B16 Melanoma 세포에서 Protein Kinase 억제제들이 Cyclic AMP 경로를 통한 멜라닌 생성에 미치는 영향)

  • 차상복;조남영;윤미연;임혜원;김경원;박영미;이지윤;이진희;김창종
    • YAKHAK HOEJI
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    • v.47 no.1
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    • pp.31-36
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    • 2003
  • To investigate the effect of protein kinase on melanin production via cAMP-dependent pathway, we measured the melanin amount and tyrosinase activity in B16 melanoma cells stimulated by alpha-melanocyte stimulating hormone (MSH), forskolin and 8-Br-cAMP. MSH, forskolin and 8-Br-cAMP significantly increased both melanin production and tyrosinase activity in B16 cells. Melanin production and tyrosinase activity by MSH are significantly inhibited by cyclic AMP-dependent protein kinase inhibitor (KT5720) and protein kinase C down-regulation treated with PMA. Bisindolmaleimide (1$\mu$M), protein kinase C inhibitor, significantly inhibited melanin production and tyrosinase activity stimulated by MSH, forskolin and 8-Br-cAMP with the following order of potency: MSH>forskolin>8-Br-cAMP. Tyrosine kinase inhibitor, genistein and DHC, significantly inhibited both, but the inhibitory effect was more potent in 8-Br-cAMP-stimulated B16 cells than MSH-stimulated cells. NFkB inhibitor (parthenolide) significantly inhibited melanin production and tyrosinase activity. Neither melanin production nor tyrosinase activity induced by MSH, forskolin and 8-Br-cAMP were affected by KN-62 (calmodulin-dependent protein kinase II inhibitor), PD098059 (mitogen-activated protein kinase inhibitor, MAPKK) and worthmannin (phosphatidylinositol 3-kinase inhibitor). These results suggest that both protein kinase C and tyrosine kinase are involved in melanin production by cyclic AMP-dependent pathway and NFkB pathway may play an important role in cyclic AMP-dependent melanin production in B16 melanoma cells.

Waste Elimination in Construction Process using Value Stream Analysis - Focused on Waste Elimination of Re-bar Works (가치흐름 분석을 통한 건설프로세스의 낭비제거 방안)

  • Mun Jeong-Mun;Kim Chang-Duk;Park Dong-Sik
    • Proceedings of the Korean Institute Of Construction Engineering and Management
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    • pp.416-421
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    • 2001
  • The domestic reinforcement concrete works have mainly worked the process of re-bar fabrication/assembly on site and re-bar works affected by structural safety, durability, and schedule with form work. Accordingly, This study analyzes the process of re-bar fabrication/assembly on site to apply lean production principles to construction Value Stream Analysis(VSA) is analyzed into value-adding activity and non-value-adding activity on construction process through value analysis and Value Stream Mapping(VSM). In the results, non-value-adding activity generates waste such as the activity steps, labors, equipments, materials, time, and so on. Additionally, push-driven production is investigated making low productivity from the overproduction and so on. To resolve the problems in the process, The purpose of this paper eliminates waste factor through maximizing the value-adding activity generating value added and minimizing non-value adding activity. Particularly, it makes flow production and pull-driven production through minimizing work-in-process(WIP ).

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Determination of Medium Components in the Flocculating Activity and Production of Pestan Produced by Pestalotiopsis sp. by Using the Plackett-Burman Design

  • Moon, Seong-Hoon;Hong, Soon-Duck;Kwon, Gi-Seok;Suh, Hyun-Hyo;Kim, Hee-Sik;An, Keug-Hyun;Oh, Hee-Mock;Mheen, Tae-Ick;Yoon, Byung-Dae
    • Journal of Microbiology and Biotechnology
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    • v.8 no.4
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    • pp.341-346
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    • 1998
  • Optimization for the production of Pest an was followed by the Plackett-Burman Design, using modified Czapek-dox medium as the starting point. At the flask level, $K_2HPO_4$, $MgSO_4{\cdot}7H_2O$, and aeration variables positively affected the Pestan production, DCW (dry cell weight), apparent viscosity, and flocculating activity response. KCI and $FeSO_4{\cdot}7H_2O$ negatively affected the Pestan production, DCW, apparent viscosity, and flocculating activity response. Aeration variable was shown to have a positive effect on only the flocculating activity response among Pestan production, DCW, and apparent viscosity responses. In comparison of the positive and negative variables media conditions, Pestan production and flocculating activity differed by about 9 and 125 times, respectively. In particular, at the jar fermentor level, the aeration variable was the most important factor of the all responses (pestan production, DCW, apparent viscosity, flocculating activity, and anionic charge density). The flocculating activity and apparent viscosity of Pestan were closely related to the molecular chain length and charge density.

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An Analysis on the Causality between Production Activity and Electricity Consumption in Manufacturing Sector (제조업 생산활동과 전력소비 간의 인과관계 분석)

  • Lim, Jaekyu;Kim, Jong-Ik
    • Environmental and Resource Economics Review
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    • v.23 no.2
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    • pp.349-364
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    • 2014
  • This study analyzed Granger causality between power consumption and production activity in manufacturing sector, by using error correction model. It found that there exists the connection between power consumption and production activity in manufacturing sector. By reflecting the industrial characteristics, it found not only the bilateral causality (power consumption ${\leftrightarrow}$ production activity) in power non-intensive industry, high value-added industry and low value-added industry, but also one-way causality (power consumption ${\rightarrow}$ production activity) in power-intensive industry. These results imply that power demand management policy focusing on efficiency improvement is necessary primarily to minimize negative impacts on production activity, and also stable power supply system is required to meet the increase of power demand.

Effects of Phenylpropanoid Compounds on Melanin Production in B16 Melanoma Cells (B16 Melanoma 세포에서 Phenylprlopanoid 화합물이 Melanin 생성에 미치는 영향)

  • 박영미;윤미연;김경원;조남영;임혜원;이지윤;이진희;김연정;김창종
    • YAKHAK HOEJI
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    • v.47 no.6
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    • pp.398-403
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    • 2003
  • To investigate the relationship between structure and biological activity of phenylpropanoids, we measured effects of phenylpropanoids on anti-oxidant and whitening activity, In DPPH radical scavenging activity, caffeic acid analogues showed the significant anti-oxidant activity. Although phenylpropanoids did not inhibit purified-tyrosinase activity, they significantly inhibited tyrosinase activity and melanin production in MSH-stimulated B16 melanoma cells. However, phenylpropanoids did not affect tyrosinase expression in MSH-stimulated B16 melanoma cells, which suggest that inhibition of MSH-induced melanin production was due to tyrosinase inhibition mediated via other signal pathways but not expression of tyrosinase. Phenylpropanoids also significantly inhibited both hyaluronidase and elastase activity, suggesting that phenylpropanoids may be used as whitening, hydration and anti-wrinkling agents. Hydroxyl residue of aromatic ring in phenylpropanoids plays an important role in anti-oxidant and whitening activity.

Effect of Chitosan Oligosaccharide on Melanin Production in B16 Melanoma Cells (B16 Melanoma 세포에서 Chitosan Oligosaccharide가 Melanin 생성에 미치는 영향)

  • 조남영;윤미연;김경원;박영미;임혜원;이지윤;이진희;김연정;김창종
    • YAKHAK HOEJI
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    • v.47 no.6
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    • pp.404-409
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    • 2003
  • To investigate the effect of chitosan oligosaccharide on melanin synthesis, we measured tyrosinase activity and melanin production in B16 melanoma cells. Chitosan oligosacchaide itself did not have any anti-oxidant activity in DPPH radical scavenging, and did not affect the proliferation of B16 melanoma cells. Chitosan oligosaccharide dose-dependently increased melanin production in the absence or presence of MSH. However, chitosan oligosaccharide did not have any influence on the tyrosinase activity and tyrosinase expression in B16 melanoma cells. These results suggest that chitosan oligosaccharide-induced melanin production may be independent on tyrosinase activity in B16 melanoma cells. From the above results. chitosan oligosaccharide dose-dependently appears to increase melanin production in B16 melanoma cells, suggesting that chitosan oligosaccharide may be used as a tanning agent.

Augmentation of Macrophage Antitumor Activities and Nitric Oxide Production by Oregonin

  • Joo, Seong-Soo;Kim, Han-Jun;Kwon, Hee-Seung;Lee, Do-Ik
    • Archives of Pharmacal Research
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    • v.25 no.4
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    • pp.457-462
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    • 2002
  • Oregonin, a diarylheptanoid derivative from Alnus hirsuta Turcz, Betulaceae, was evaluated for its antitumor activity. Oregonin, known to have an antitumor function, and is a novel immunomodulator, which may augment macrophage activity. MTT assays and NO production tests were performed in order to investigate the cytotoxicity of oregonin in tumor cells and to examine its influence on macrophage in detail. In this study, the tumoricidal activity was also evaluated by a MTT assay. The cytotoxicity measurements in the oregon in-treated group both in vitro and in vivo showed a significant difference from that of the control group. In vivo, oregonin significantly increased NO production in a dose-dependent manner, and in vitro, the thioglycolate-induced inflammatory macrophages increased NO production in a dose-dependent manner after incubation. These results suggest that oregonin reacts with both the inflammatory and non-inflammatory macrophages in a similar way.