• Title, Summary, Keyword: purification method

Search Result 749, Processing Time 0.054 seconds

Isolation and purification of chicken egg yolk immunoglobulin against Edwardsiella tarda (Edwardsiella tarda에 대한 계란난황항체의 분리와 정제)

  • Kim, Yeong-Dae;O, Myeong-Ju;Jeong, Tae-Seong;Jeong, Seong-Ju
    • Journal of fish pathology
    • /
    • v.17 no.1
    • /
    • pp.11-20
    • /
    • 2004
  • The present study compared purification methods of hen egg yolk immunoglobulin (IgY) from the hen immunized with Edwardsiella tarda. The purification of anti-E. tarda IgY was performed by four different methods, polyethylene glycol (PEG), chloroform polyethylene glycol (Chloroform-PEG), ammonium sulfate and purification kit. Purified IgY had heavy chain of 64 kDa and light chain of 27 kDa size. IgY purified from the hen immunized with E. tarda showed higher ELISA values and agglutination titers than those with IgY purified from the non-immunized hen as a negative control. In addition, purified IgY recognized similar E. tarda proteins to those with anti-E. tarda rabbit serum by western blotting. Purified IgY had an agglutination titer of 1:512 by PEG method and ammonium sulfate method, and 1:128 by chloroform-PEG method and purification kit. Moreover, PEG method was the most rapid method among the four different IgY purification methods. These results indicate that PEG method is effective purification method maintaining biological activity of the IgY.

Novel Purification Method of Kv 4.2 Potassium Channel from Rat Brain Membrane

  • Park, Sung-Soo
    • Biomedical Science Letters
    • /
    • v.18 no.2
    • /
    • pp.96-103
    • /
    • 2012
  • Kv 4.2 ion channel protein has an ability to open at subthreshold membrane potentials and to recover quickly from inactivation. That is very important for neuronal signal transmission in vertebrate brain. In order to purify Kv 4.2 protein, the novel purification methods were experimented. The purification procedure utilized chromatography on DE-52 ion exchange column and affinity chromatography on a WGA-Sepharose 4B, and Kv 4.2 affinity column chromatography. It was found that 0.5% (wt./vol.) Triton X-100 detergent in lysis buffer worked well for Kv 4.2 protein solubilization from rat brain membrane. Protein quantitative determination was conducted by BCA method at 562 nm for each purification step to avoid determination interference of protein at 280 nm by detergent. The confirmation of Kv 4.2 existence and amount is performed using by SDS-PAGE/immunoblotting or 96-well dot blotting. The Kv 4.2 without interacting protein that contains carbohydrate, was purified from novel biochemical 3-steps purification method for further research.

A Study on the Solidification and Purification of High Purity Aluminium and Silicon by Stirring Method (냉각체 회전법에 의한 고순도 알루미늄 및 규소의 응고 및 정련에 관한 연구)

  • Kim, Wook;Lee, Jong-Ki;Baik, Hong-Koo;Yoon, Woo-Young
    • Journal of Korea Foundry Society
    • /
    • v.11 no.4
    • /
    • pp.303-313
    • /
    • 1991
  • The Purification mechanism of high purity aluminum was studied through the variation of stirring speed and coolant flow rate in the stirring method. In the stirring method the degree of purification was changed as the following factors;the variation of diffusion boundary layer thickness the variation of growth rate and the solute concentration of the residual melt. The concentration of Fe and Si was decreased as the stirring speed and the radial distance increased. In a high stirring speed of 2000rpm with unidirectional stirring mode, the uniformity of solutes was obtained. On the other hand, the purification of Si was done by the combinations of stirring method, fractional melting and acid leaching. In the case of Si purification, the centrifugal force developed in the melt acted as the significant purification factor. It was possible to obtain the purified 3N grade Si crystal after the complete elimination of residual aluminum by fractional melting and acid leaching.

  • PDF

A New large-scale Pre-purification for Peroxidase from Plant Cell Cultures (식물세포 배양으로부터 Peroxidase 대량 정제를 위한 전처리 공정 개발)

  • 표상현
    • KSBB Journal
    • /
    • v.15 no.4
    • /
    • pp.342-345
    • /
    • 2000
  • A novel pre-purification method was developed for producing peroxidase to guarantee high purity and yield from plant cell cultures in large-scale process. This method was a simple and efficient procedure for the isolation and pre-purification of peroxidase from the biomass consisting of active clay treatment followed by cationic exchange chromatography. The use of active clay in the pre-purification process allows for rapid and efficient separation of peroxidase from interfering compounds and dramatically increases yield and purity of crude peroxidase for purification steps compared to alternative processes. This pre-purification process serves to minimize the buffer usage size and complexity of the HPLC operations for peroxidase purification. This process is readily scalable to a pilot plant and eventually to a production environment where mass production of material are expected to be produced.

  • PDF

New Efficient Method for Isolation and Purification of Ginsenosides (Ginsenoside의 새로운 분리.정제 방법)

  • 김세원;황석연
    • Journal of Ginseng Research
    • /
    • v.22 no.4
    • /
    • pp.284-288
    • /
    • 1998
  • This study was carried out to establish a new efficient method for isolation and purification of ginsenosides. Silica gel column chromatography, having been used for the isolation of ginsenosides, is advantageous to obtain a large amount of ginsenosides. However, it has a disadvantage to isolate ginsenosides to their highest purity. In addition, normal-or reverse-phase HPLC method thus far reported is confined to quantitative analysis. Especially, it has not been possible to isolate racemic 20(S)- and 20(R)-ginsenoside Rg2. In this experiment, isolation and purification of ginsenosides were accomplished by Diaion HP-20 adsorption chromatography, silica gel column chromatography, recrystalization and Prep. HPLC with or without Prep. TLC. From this study, we could establish a new efficient method for isolation and purification of 9 major and/or minor ginsenosides.

  • PDF

Separation and Purification of Cholesterol from By-product of Low Cholesterol Egg Yolk (저콜레스테롤 난황 제조시 생성되는 부산물로부터 콜레스테롤의 분리 정제)

  • 유익종;조혜연;박우문;전기홍;최성유
    • Food Science of Animal Resources
    • /
    • v.20 no.1
    • /
    • pp.36-43
    • /
    • 2000
  • $\beta$-cyclodextrin adsorption and saponification methods were applied to isolate and purify cholesterol from the by-product of the low-cholesterol egg yolk product. They by-product was prepared from processing low-cholesterol egg yolk followed by extracting with chloroform to remove $\beta$-cyclodextrin and concentrated to 3,069 mg% cholesterol. When $\beta$-cyclodextrin method between two purification methods was applied, 50% ethanol as a solvent showed higher cholesterol concentration of 5.82% rather than the other solvents. Repeated purification of 3 times could not improve the cholesterol concentration significantly(p<0.05). In case of purification using saponification method, hexane as a solvent for extraction of unsaponificated materials was more efficient to increase cholesterol concentration than chloroform and ether. 60 times(v/w) saponification solution (95% ethanol:33% KOH = 94:6) of sample weight was most effective to increase the cholesterol concentration of 35.7%. Repeated purification process by saponification method could increase cholesterol concentration to 95.7% by 4 times repetition.

  • PDF

Development of High Performance Liquid Chromatography for Paclitaxel Purification from Plant Cell Cultures

  • Kim, Jin-Hyun;Choi, Hyung-Kyoon;Hong, Seung-Suh;Lee, Hyun-Soo
    • Journal of Microbiology and Biotechnology
    • /
    • v.11 no.2
    • /
    • pp.204-210
    • /
    • 2001
  • Paclitaxel can be produced in high yield and with a high degree of purify from plant cell cultures of Taxus chinensis. The complete purification method was systematically established and described. This method was an efficient procedure for the purification of paclitaxel from crude paclitaxel, consisting or reverse-phase chromatography, followed by a normal-phase chromatography. The two-stage HPLC purification scheme serves as an effective and economical approach for resolving paclitaxel from complex mixtures of taxoids, with high purify (>99%) and low impurities (<0.1%). The process is readily scalable to a pilot plant and eventually to a production environment where multikilogram quantities of material are expected to be produced. The process has been optimized to minimize solvent usage, complexity, and operating costs.

  • PDF

A Novel Purification Process for Homoharringtonine from Celphalotaxus koreana

  • Sung, Ju-Li;Kim, Jin-Hyun
    • 한국생물공학회:학술대회논문집
    • /
    • /
    • pp.521-524
    • /
    • 2003
  • An effective purification method was developed for producing Homoharringtonine (HHT), to guarantee high purity and yield from Cephalotaxus koreana. This process was a simple and efficient procedure, for the isolation and purification of HHT form the biomass of Cephalotaxus koreana, consisting of extract, adsorbent treatment, precipitation and followed by a chromatography. The extraction, adsorbent treatment and precipitation in pre-purification process allows for rapid and efficient separation of HHT from many compound and dramatically increases the yield and purity of crude HHT for HPLC purification steps compared to alternative processes. This purification processes serves to minimize solvent usage, size, and complexity of the operations for HHT purification.

  • PDF

Determination of self-purification constants and regulation of pollutants loaded in the ecosystems (生態系에 있어서 自淨係數의 測定과 汚染負荷量의 調節 原理)

  • Chang, Nam-Kee;Kim, Jae-Young
    • The Korean Journal of Ecology
    • /
    • v.15 no.3
    • /
    • pp.287-296
    • /
    • 1992
  • To determinate self-purification constants of pollutants loaded in the ecosystems, the self- purification process was formulated, and a measurement method of the self-purification constants was derived. $C=C_0e^{-st}$ When $C_0$ is the initial pollutant amounts loaded in a ecosystem, and C is the rest pollutant amounts after the time, t, the equation of the self-purification, s, is $s=\frac{P}{C}$ When in aquatic ecosystem, $C_0$ is the initial polluant amounts loaded in water body, and Cis the rest pollutation amounts after the time, t, the self-purification constant, s, is $s=(\frac{\ln C_0-\ln C}{t}$ Self-purification constants of pine and oak forests at kwangneung in kyonggido were 0.07 and 10 respectively, of BOD in gokneung stream in kyonggido was 0.51, and of glucose and phosphate in pools on the stone in mt.jiri were 0.49 and 15.19 respectively.

  • PDF

Paclitaxel : Recovery and Purification in Commercialization Step (Paclitaxel : 산업화 단계에서의 회수 및 정제)

  • Kim Jin-Hyun
    • KSBB Journal
    • /
    • v.21 no.1
    • /
    • pp.1-10
    • /
    • 2006
  • The recovery and purification of a paclitaxel from plant cell cultures is essential to commercial process. This review describes a large-scale recovery and purification method for producing paclitaxel, to guarantee high purity and yield from plant cell cultures. Also, the process of separation and purification is optimized in conjunction with a extraction step, pre-purification, purification, and polishing (drying) as an integrated process to meet final product quality requirements such as purity, residual solvents, product morphologies, impurities, bacterial endotoxin, etc. This information is very useful for production and quality control of pharmaceuticals in commercialization step.