• Title, Summary, Keyword: reticuloendotheliosis

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Differential diagnosis among Marek's disease, reticuloendotheliosis and avian leukosis by polymeras chain reaction (중합효소연쇄반응을 이용한 닭 종양성 질병의 감별진단에 관한 연구)

  • Seong, Hwan-woo;Kim, Sun-jung
    • Korean Journal of Veterinary Research
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    • v.38 no.1
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    • pp.101-106
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    • 1998
  • The present study attempted to apply polymerase chain reaction (PCR) to develop a rapid differential diagnosis among Marek's disease, reticuloendotheliosis and avian leukosis. The primers chosen to detect Marek's disease virus (MDV) flank the 132bp tandem direct repeat of the MDV genome. The primers selected for reticuloendotheliosis virus (REV) and avian leukosis virus (ALV) are based on proviral long terminal repeats of spleen necrosis virus and Rous-associated virus-2 genomes, respectively. The specific PCR products of MDV, REV and ALV were observed with each primer and the reaction was not cross-reacted among the viruses. MDV-specific DNA was also amplified from the MDV-induced lymphoma (MDCC-MSB1) but not from the REV-induced tumor and ALV-induced lymphoma (LSCC-1104B1). In addition, proviral DNA of REV from REV-induced tumor and proviral DNA of ALV from ALV-induced lymphoma were also amplified by REV-specific and ALV-specific PCRs, respectively. Therefore these three PCR methods may be used to rapidly differentiate among MDV, REV and ALV-associated tumors in diagnosis.

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Seroprevalence of reticuloendotheliosis virus infection in layer chickens in Gyeonggi province, South Korea (경기도 산란계의 세망내피증 바이러스 항체 양성률 조사)

  • Jung, Kwang
    • Korean Journal of Veterinary Service
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    • v.43 no.3
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    • pp.197-200
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    • 2020
  • This study was performed to determine the seroprevalence of reticuloendotheliosis virus (REV) infection in layer chickens in Gyeonggi province, South Korea. Serum samples were collected from 820 layer chickens on 56 flocks in Gyeonggi province. The samples were tested for specific antibodies against REV using commercial enzyme-linked immunosorbent assay (ELISA). The seroprevalence of REV antibodies in the flocks was 62.50% (95% Confidence interval (CI) 49.33~74.40) and the overall seroprevalence in individual chickens was 39.27% (95% CI 35.97~42.65). The results of the present survey indicate that REV infection is prevalent in layer chickens in Gyeonggi province, South Korea. Therefore, effective measures should be taken to prevent and control REV of layer chickens in this province.

Immunosuppressive effects and pathogenicity of a Korean isolate of reticuloendotheliosis virus in chickens (Reticuloendotheliosis virus의 닭에 대한 면역억제효과와 병원성)

  • Han, Myung-guk;Kim, Sun-joong
    • Korean Journal of Veterinary Research
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    • v.40 no.2
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    • pp.311-323
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    • 2000
  • Immunosuppressive effects of reticuloendotheliosis virus (REV) infection in chickens were investigated. Primary antibody responses to Newcastle disease virus (strain B1) and sheep red blood cells were significantly low in chickens inoculated with the local isolate 89-74 of REV compared to those of uninfected chickens. In chickens infected with REV strain T or 89-74, blastogenesis of spleen cells and peripheral blood lymphocytes (PBL) to concanavalin A (Con A) was severely suppressed. When specific pathogen free (SPF) chickens were inoculated with the isolate, the suppressive effect was observed up to 7 weeks of age while, in the contact infected chickens, the suppression was absent. Similar suppressive effects were observed in chickens inoculated with REV strain T at 2, 3 and 4 weeks of age. When spleen cells or PBL from uninfected chickens were co-cultured with spleen cells or PBL from chickens infected with REV at 1 day-old or 2 week-old, the blastogenesis of the normal cells was suppressed. The suppressive effect of PBL from REV-infected chickens on normal lymphocytes was abrogated by the treatment with trypsin. However the suppressive activity of the REV-infected PBL was not influenced at removing machrophage from the cell suspension by incubation in plastic petri dishes. In addition to the immunosuppression, chickens infected with the REV isolate showed abnormal feather development (nakanuke), anemia, paralysis and retarded growth. Three out of 11 chickens inoculated with the isolate at day-old died between 6 and 9 weeks of age by bacterial infections.

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Immunosuppressive effects of a Korean isolate of reticuloendotheliosis virus (국내 분리 세망내피증 바이러스의 면역억제능)

  • Seong, Hwan-woo;Kim, Sun-jung
    • Korean Journal of Veterinary Research
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    • v.38 no.4
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    • pp.811-817
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    • 1998
  • Humoral and cellular immune responses are depressed in chickens infected with reticuloendotheliosis virus(REV). The extent of depression is influenced by the age of infection and strain of virus. This study was conducted for investigation of immunosuppressive effects of a Korean isolate of REV. Chickens infected with REV-HI, a Korean isolate, at 1 day old were severely suppressed in the vaccinal immunity against Newcastle disease, infectious bronchitis and infectious bursal disease. But these immunosuppressive effects were not observed in chickens infected with the virus at 2 weeks of age, or contact infected by growing in-contact with inoculated chickens from one day old. The clinical signs following infectious laryngotracheitis(ILT) vaccination in chickens infected with REV-HI at 1 day old were more severe than those of uninfected chickens, and some of REV-infected chickens(21.4%) were died after the vaccination. Mortality following virulent ILT virus infection was increased in REV-HI infected chickens. Effects of REV infection at one day old to susceptibilities to subsequent Chicken anemia agent (CAA) infection were also studied. Chickens were infected with REV-HI at 1 day old and subsequently inoculated CAA at 1, 7, 14 and 28 days old, respectively. Mortalities of the chickens infected with REV-HI and subsequent CAA infection were 100, 100, 40 and 0%, respectively, whereas 23, 8, 0 and 0% of chickens infected with only CAA were died, respectively. These above all results suggest that a Korean isolate of REV may be highly immunosuppressive.

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Clinical blood chemistry analysis in chickens infected with reticuloendotheliosis virus (세망내피증 바이러스 감염 닭에서의 혈액화학치 변화)

  • Sung, Haan-Woo;Kwon, Hyuk-Moo;Kim, Sun-Jung
    • Korean Journal of Veterinary Research
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    • v.48 no.4
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    • pp.451-455
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    • 2008
  • Body weights and blood biochemical values in chickens infected with reticuloendotheliosis virus (REV)-HI, a Korean isolate, were studied. REV-HI causes severe body weight depression in chickens inoculated but not in chicken contact-infected. Body weights of infected chickens in 3, 4, and 5 weeks after infection were 78%, 76% and 65% of those of control respectively. Blood glucose levels in REVinfected chickens were extremely high compared with those in control (226 $\geq$ 21 vs. 814 $\geq$91.3 mg/dl in week 2) during the experiment period. Triglyceride levels in REV-infected chickens were significantly higher in week 2 and 3, whereas in week 4, REV-infected chickens showed significantly lower levels than the control. Blood lipase, amylase and alkaline phosphatase levels of REV-infected chickens in week 2 were significantly higher, whereas cholesterol, magnesium and calcium values in week 4 were significantly lower than the control. Other blood biochemical values such as alkaline aminotransferase, aspartate aminotransferase, and $\gamma$-glutamyltransferase were nonsignificantly different from the control. These above results suggest that weight depression by REV may be related with increase of blood glucose, which indicated that REV-infected chickens could not use blood glucose as energy source.

Reticuloendotheliosis (세망내피증)

  • 김순재
    • KOREAN POULTRY JOURNAL
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    • v.12 no.6
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    • pp.54-57
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    • 1980
  • 세망 내피증은 최근 알려지기 시작하고 있는 가금류에 종양을 일으키는 종양성 질병으로 그 증상이 백혈병과 마렉크병과 유사하여 감별진단이 어렵고 실험적으로 30$\%$정도의 폐사율을 가지고 있는 질병이다.

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Reticuloendotheliosis; RE (세망 내피증)

  • 김순재
    • KOREAN POULTRY JOURNAL
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    • v.15
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    • pp.108-112
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    • 1983
  • 본 병은 세망내증 바이러스로 인하여 조류에 감염되는 종양성 전염병으로서 수평감염을 하고 있으므로 관리인, 양계기구 및 닭상인의 위생관리를 철저히 실시해야 한다.

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Standardization of an enzyme-linked immunosorbent assay for detection of antibody to avian reticuloendotheliosis virus (세망내피증 바이러스 항체검출을 위한 ELISA 표준화)

  • Sung, Haan Woo;Lee, Su Jeong
    • Korean Journal of Veterinary Research
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    • v.45 no.4
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    • pp.569-574
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    • 2005
  • Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to reticuloendotheliosis virus (REV) at single serum dilution was standardized. REV HI, one of the Korean field isolates, was inoculated into chicken embryo fibroblast (CEF) cells and was harvested from the culture fluids and cells after 10 to 12 days. Viruses were purified by centrifugation at the $107,000{\times}g$ for 12 hours on 20, 30, 45% (W/V) sucrose gradient. Virus specific fraction was collected and used as ELISA antigen. To standardize ELISA, the optimal concentration of coating antigen ($1{\mu}g/well$) and conjugate (1/1000) was determined by corrected OD (OD value of positive serum-OD value of negative serum) and P/N ratio (OD value of positive serum/OD value of negative serum). To calculate ELISA titer by measuring absorbance at 1/400 single serum dilution, serum titrations were carried out for various sample sera together with standard positive and negative sera. The observed titers of serum samples were plotted against sample/positive (s/p) ratios at 1/400 serum dilution. From the above data, the ELISA titers could be calculated by the equation of $log_{10}$ ELISA titer = 2.2763 ($log_{10}$ s/p) + 3.482 (r = 0.93). For evaluating the sensitivity, the standardized method were compared with conventional agar gel immunodiffusion (AGID) test method using serum samples collected from REV infected field chicken flocks. Fifty seven of 60 samples (95%) were positive for REV by ELISA, whereas only 11 (18.3%) samples were positive by AGID test. This results suggested that the ELISA tests developed in this study could be used for detection of antibodies to REV with high sensitivity.

Analysis of antibody titer of transovarian transmitted diseases in Korean native breeder chickens (토종닭 종계의 난계대 전염병 항체 양성률 분석)

  • Park, Ki-Tae;Kim, Yeon-Ha;Kang, Bo-Seok;Kang, Hwan-Ku;Hong, Eui-Chul;Kim, Hyun-Soo;Jeon, Jin-Joo;Son, Ji-Seon;Kim, Chan-Ho;Kim, Sang-Ho;Choe, Changyong
    • Korean Journal of Veterinary Service
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    • v.43 no.3
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    • pp.139-145
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    • 2020
  • This study was carried out to identify the occurrence of transovarian transmitted diseases and antibody positive rates among Korean native breeder chickens. The infection rates with Salmonella pullorum and Salmonella gallinarum among 16-week-old, 36-week-old, and 56-week-old breeder chickens and the antibody positive rates to Egg Drop Syndrome '76, Mycoplasma gallisepticum and Mycoplasma synoviae among 16-week-old, 18-week-old, and 56-week-old breeder chickens were identified, and the antibody positive rates to seven major transovarian transmitted diseases among 1-day-old chicks were investigated. As a result, no infection with Salmonella pullorum and Salmonella gallinarum was found among the investigated subjects of all ages. Vaccinated breeder chickens showed the 100% antibody positive rate to Egg Drop Syndrome '76, and unvaccinated breeder chickens showed the 100% antibody negative rate to Mycoplasma gallisepticum and Mycoplasma synoviae, confirming that there was no infection with Mycoplasma gallisepticum and Mycoplasma synoviae. As a result of the antibody tests of the 1-day-old chicks for transovarian transmitted diseases, it was found that vaccinated chicks showed good antibody positive rates to avian encephalomyelitis, chicken infectious anemia, and avian reovirus, confirming that they had power of defense against the relevant infectious diseases, and that unvaccinated chicks showed the 100% antibody negative rates to avian leukosis, chicken reticuloendotheliosis, and Mycoplasma synoviae, confirming that there was no infection with the relevant diseases. Given that the results of this study showed that among the transovarian transmitted diseases of chickens, there was no history of infection with diseases against which vaccination was not administered and high antibody positive rates were found with diseases against which vaccination was administered, it is judged that chickens with good power of defense against diseases were bred, and it is deemed that constant monitoring and vaccination against transovarian transmitted diseases will be necessary for the control and prevention of the diseases.