• Title, Summary, Keyword: rosette formation

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Immunobiological Studies in Mice Treated with Chemical Carcinogen, 3-Methylcholanthrene : II. Rosette Formation and Natural Killing (NK) Activity of Splenic Lymphocytes (발암제(發癌劑) 3-Methylcholanthrene 투여(投與)마우스에 대(對)한 면역생물학적(免疫生物學的) 연구(硏究) : II. 비장세포(脾臟細胞)의 Rosette형성능(形成能) 및 NK세포(細胞)의 활성(活性))

  • Song, Hee-jong;Kim, Sang-ho;Kim, Jong-myeon
    • Korean Journal of Veterinary Research
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    • v.26 no.1
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    • pp.117-124
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    • 1986
  • The present study was undertaken to evaluate rosette formation and NK activity of splenic lymphocytes in 3-methylcholanthrene (MCA) treated mice. Mice were sensitized iv with 0.1ml of 1% sheep red blood cell (SRBC) suspension were treated with a single ip injection of olive oil alone or with different doses of MCA in oil at various time before or after sensitization, and were challenged at 4 days after SRBC. Rosette formation and NK activity of splenic lymphocytes were measured at 24 hours after challenge. Erythrocyte(E) rosette formation of splenic lymphocytes was significantly depressed in mouse treated with large dose of MCA (5~50mg) regardless of injecting time. But, there was no difference in the response between the treated with small dose of MCA (0.5mg). Whereas erythrocyte-antibody(EA) rosette or erythrocyte-antibody-complement(EAC) rosette forming cells were significantly depressed by MCA. Under small dose of MCA (0.5mg), any difference of NK activity was not observed in all course of injecting time. But, under large dose of MCA, the activity was markedly inhibited to about half the values seen in control and this suppression was transient, resulting that the normal level was reached again 19 days after MCA. These results, which conform with the predictions of immunosuppression hypothesis, suggest that MCA inhibits immunological responses including NK activity and thereby allows the outgrowth of antigenic neoplastic cells.

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Effect of PHA and conditioned medium on blastogenesis and rosette formation of bovine circulating blood lymphocytes (PHA 및 conditioned medium 이 소의 순환혈액 림프구의 유약화와 rosette 형성에 미치는 영향)

  • Kang, Sei-woong;Yoon, Chang-yong;Song, Hee-jong
    • Korean Journal of Veterinary Research
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    • v.34 no.2
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    • pp.301-306
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    • 1994
  • This study was planned to estimate the activity of bovine circulating blood lymphocytes using phytohemagglutinin-M(PHA) known as T cell mitogen. Bovine circulating blood mononuclear cells(MNCs) was separated, and cultured with or without macrophage($PHA^+/M{\phi}^+$ or $PHA^+/M{\phi}^-$) in conditioned medium which stimulated with various concentration of PHA(0, 5, 10, 15 and $20{\mu}g/ml$ in medium), and then investigated the blastogenic response and rosette formation of lymphocytes. Blastogenic rate(BR) was especially increased in PHA concentration(10 and $15{\mu}g/ml$) of $PHA^+/M{\phi}^+$ group and their BR were $41.5{\pm}6.8%$ and $44.4{\pm}8.9%$, respectively and BR in PHA concentration(15 and $20{\mu}g/ml$) of $PHA^+/M{\phi}^-$ group was $32.8{\pm}6.2%$ and $31.4{\pm}4.6%$, respectively. BR of lymphocytes was more increased in $PHA^+/M{\phi}^+$ than $PHA^+/M{\phi}^-$ group when these cells were stimulated by PHA. Rosette forming rate(RFR) of lymphocytes to SRBC highly increased when SRBC was treated with AET and/or dextran, respectively. On the orther hand, RFR significantly increased more in $PHA^+/M{\phi}^+$ and $PHA^+/M{\phi}^-$ group than in control group, but when compared with two groups, statistical significancy was recognized only in PHA concentration($15{\mu}g/ml$, p<0.026) of $PHA^+/M{\phi}^+$ group.

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The kinematic properties of stellar groups in the Rosette Nebula: its implication on their formation process

  • Lim, Beomdu;Hong, Jongsuk;Naze, Yael;Park, Byeong-Gon;Hwang, Narae;Lee, Jeong-Eun;Yun, Hyeong-Sik;Park, Sunkyung;Yi, Hee-Weon
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.51.1-51.1
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    • 2020
  • The Rosette Nebula is the most actively star-forming region in the Monoceros OB2 association. This region hosts more than three stellar groups, including the most populous group NGC 2244 at the center of the region and the smaller stellar groups around the border of the H II bubble. To trace their formation process, we investigate the kinematic properties of these groups using the Gaia astrometric data and high-resolution spectra taken from observation with Hectochelle on MMT. The proper motions of stars in NGC 2244 show a pattern of radial expansion. The signature of cluster rotation is also detected from their radial velocities. On the other hand, the small groups appear to be physically associated with some clouds at the ridge of the H II region. Among them, the group near the eastern pillar-like gas structure shows the signature of feedback-driven star formation. In this presentation, we will further discuss the formation process and dynamical evolution of the stellar groups in the Rosette Nebula, based on the observation and results of N-body simulations.

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Studies on the Activity of Immune Regulatory Cells in the Korean Native Cattle : I. E Rosette Formation Rate of Peripheral Blood Lymphocytes (한우(韓牛)에서 면역조절세포(免疫調節細胞)의 활성(活性)에 관한 연구(硏究) : I. 말초혈액(末梢血液) 임파구(淋巴球)의 E Rosette 형성능(形成能))

  • You, Nam-sun;Kim, Jong-myeon;Song, Hee-jong;Chai, Hyo-seok;Kang, Myeong-dai;Lee, Ju-mook
    • Korean Journal of Veterinary Research
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    • v.27 no.2
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    • pp.253-258
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    • 1987
  • The erythrocytes(E) rosette forming capacity of peripheral blood lymphocytes(PBL) in Korean native cattle was determined with 2-aminoethylisothiouronium bromide hydrobromide(AET) and dextran(Dex)-treated sheep erythrocytes(SRBC). To further standardize the assay, optimum concentration of AET and/or Dex-treatment and incubation time for rosette forming cell(RFC) counts were determined. In untreated SRBC resuspended in the rosetting medium(RPMI 1640 containing 10% FCS), PBL from 7 animals formed low percentage of rosettes($7.8{\pm}6.0%$). Both AET and Dex treatment not only enhanced the rosette formation but also made it easy to enumerate rosettes by increasing numbers of SRBC attached on them. SRBC treated with 0.1M AET for 20 min or 8% Dex formed the highest percentages of rosettes, ($35.7{\pm}6.0%$ and $48.3{\pm}4.7%$, respectively) and were used in subsequent studies. With SRBC treated with 0.1M AET for 20 min and suspended in 8% Dex, the maximum RFC observed were $56.6{\pm}6.8%$ in female and $65.8{\pm}6.3%$ in male and the rates of RFC did not change significantly between 3 and 20 hr incubation time at ${4^{\circ}C}$.

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The Effect of Ginseng Petroleum Ether Fraction on Immunosuppressed Mice by Lead acetate (II) II. Cellular Immune Response and Histological Studies (납의 면역독성에 미치는 인삼의 영향(II) II. 세포성면역 및 조직학적 검사)

  • 김휘배;안영근;김주영;문재규
    • Environmental Analysis Health and Toxicology
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    • v.1 no.1
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    • pp.37-46
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    • 1986
  • Experiments were performed to investigate the effect of Panax ginseng petroleum ether fraction on delayed type hypersensitivity, rosette formation, phagocytic activity and histophathological influence in lead acetate treated mice. Lead acetate was administered in the drinking water and ginseng pet. ether fraction was injected i.p.. Mice were sensitized and challenged with sheep red blood cells. Erythrocyte(I) rosette formation and DTH reaction were significantly depressed in lead acetate treated mice, and those were restored administration of ginseng fraction. Ginseng pet. ether fraction administration did not have any effect on decreased phagocytic activity. Follicular and parafollicular areal destruction of spleen, and destruction of thymus were finded in lead acetate exposed-mice. Small dose of ginseng pet. ether fraction (5 mg/kg, 10 mg/kg), administraction inhibited those histopathological changes, but large dose (20 mg/kg) didn't.

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Influence for Carcinoma Cell and Lymphatic Cell of Acetyl Arsonate (아세틸 아르소네이트의 면역세포와 암세포에 미치는 영향: L1210, Sarcoma 180, MOLT-4 등 사람과 생쥐에 대한 작용비교)

  • Jeong, Yong-Ja;Seong, Yeong-Gi
    • YAKHAK HOEJI
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    • v.40 no.5
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    • pp.599-607
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    • 1996
  • Acetylarsonate was prepared for testing antitumor and immunological effects. It showed cytotoxicity directly on Sarcoma 180. L1210 and MOLT-4 by MTT assay. It did not seemed to trigger the mitosis of human lymphocytes in culture, but that showed the cytotoxicity with higher dose. The rosette formation and spleen weight of mouse which acetylarsonate was administered to for 2 weeks were increased. Furthermore, peripheral helper T- and cytotoxic/suppressor T-lymphocytes were increased in acetylarsonate-injected-mice significantly when it was estimated with simultaneous 2 color analysis using anti Lyt2-FITC and L3T4-PE monoclonal antibody by Flow cytometer.

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The response of peripheral blood lymphocytes against in vivo stimulation with mitogen in carp, Cyprinus carpio (Mitogen 투여에 대한 잉어 순환혈액 림프구의 반응)

  • Cho, Mi-Young;Park, Soo-Il
    • Journal of fish pathology
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    • v.9 no.1
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    • pp.95-109
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    • 1996
  • This work was carried out to investigate the functional heterogeneity of peripheral blood lymphocytes(PBLs) in carp, Cyprinus carpio. PHA, Con A, LPS and BCG were injected intraperitoneally into carp to determine the blastogenic response and rosette formation activity. In each group of fish treated with stimulators, the cell numbers and DNA contents of lymphocytes were higher than those of untreated control group and reached the highest level between 1 week and 2 weeks after injection with mitogens. These results showed that BCG and Con A were strong stimulators of proliferation compared to PHA and LPS. However, PHA-treated fish twice showed the highest rosette formation response among the consecutive stimulations with the same mitogen. Alase, the results on consecutive mitogen stimulation revealed that carps reinjected by different mitogens led to an increased stimulation higher than the one reinjected after 1 week with same mitogen. It seems that different mitogens may stimulate different cell populations and implies functionally separated subpopulations of lymphocytes in carp.

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Effects of Coicis Semen on the Immune Responses in the Mouse (의이인(薏苡仁)의 투여(投與)가 마우스의 세포성(細胞性) 및 체액성(體液性) 면역기능(免疫機能)에 미치는 영향(影響))

  • Woo, Young-Eun;Kim, Hyeong-Kyun;Song, Bong-Keun;Lee, Eon-Jeong
    • Korean Journal of Oriental Medicine
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    • v.2 no.1
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    • pp.269-288
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    • 1996
  • Coicis Semen is one of the oriental medicine that has been used for the treatment of the diseases such as pulmonary abscess, periappendicular abscess and wart since ancient times. However, the mechanism of the action of the drug is not well studied. This study was done to investigate the effects of Coicis Semen on the host defence mechanism. Effects of Coicis Semen on the immune responses were analysed by measuring the contact hypersensitivity, hemagglutinin, hemolysin and rosette formation, cytotoxicity, and reactive oxygen intermidiates production. As the results, water extract of Coicis Semen administration enhanced the antibodies (hemagglutinin and hemolysin) formation and the appearance of rosette forming cells of the spleen. Also Coicis Semen increased the allogeneic immune response in the mouse, showed cytotoxic activity against human leukemia cell line(K562) and decreased the contact hypersensitivity against dinitroflurobenzene. Also administration of Coicis Senlen slightly increased NK cell activity and enhanced the production of such reactive oxygen intermediates as superoxide and hydrogen peroxide from the macrophages in vivo and in vitro. The above results demonstrate that Coicis Semen has enhancing effects on cellular and humoral immune responses against disease.

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