• 제목, 요약, 키워드: salicylic acid

검색결과 325건 처리시간 0.039초

대사유도물질 처리에 의한 발아녹두의 아이소플라본 생합성 양상 (Manipulating Isoflavone Levels in Mungbean Sprouts by Chemical Treatment)

  • 이지현;정일민;박세준;김욱한;김소연;김진애;정우석
    • 한국작물학회지
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    • v.49 no.6
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    • pp.528-532
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    • 2004
  • 발아 녹두에 세 가지 스트레스 관련 화합물 salicylic acid, methyl jasmonic acid, acetyl salicylic acid를 처리하여 isoflavone의 생합성양상을 관찰한 결과를 요약하면 다음과 같다. 1. 숙주나물의 자엽에서는 isoflavone총량이 건조중 1g당 $832.5{\mu}g$인 무처리구와 비교하여 10mM salicylic acid를 처리한 경우 $169\%$, 12mM acetyl salicylic acid로 처리한 경우 $165\%$의 isoflavone 총량이 증가한 반면 $0.5\%$ methyl jasmonic acid를 처리한 경우는 오히려 무처리구보다 $47\%$ 수준으로 감소하였다. 2. 숙주나물의 자엽하부(hypocotyl and root)의 isoflavone 생성량에서는 1g당 284.8${\mu}g$이 생성된 무처리구와 비교하여 세 가지 처리 모두에서 유의성이 있는 차이를 보였다. 10mM salicylic acid 처리구의 경우 $419\%$, 12mM acetyl salicylic acid 처리구의 경우 $401\%$의 isoflavone 총량의 증가를 보였고, $0.5\%$ methyl jasmonic acid처리구의 경우에는 $121\%$증가하였다. 3.숙주나물의 자엽부위와 자엽하부에서 검출된 isoflavone의 합을 각 처리별 isoflavone생산총량으로 하여 무처리구의 건조중 1g당 1117.3${\mu}g$을 기준으로 비교하여보면 건조중 1g당 10mM salicylic acid 처리구에서는 2601.02${\mu}g$으로 $233\%$ 증가하였고, 12mM acetyl salicylic acid 2514.4${\mu}g$으로 $225\%$ 증가한 반면, $0.5\%$ methyl jasmonic acid 처리구에서는 738.8${\mu}g$으로 $66\%$ 수준으로 감소하였다. 4. 숙주나물 자엽부위의 경우 무처리구와 비교하여 증가를 보였던 10mM salicylic acid처리구와 12mM acetyl salicylic acid 처리구에서는 malonyldaidzine과 malonylglycitin이 증가가 두드러지게 나타났다. 5. 숙주나물 자엽하부의 경우 무처리구와 비교하여 증가를 보였던 10mM salicylic acid 처리구와 12mM acetyl salicylic acid 처리구에서는 malonylglycitin의 증가가 두드러지게 나타났다

Simultaneous Determination of Salicylic Acid and Aspirin in Commercial Aspirin Tablets

  • Kim, Chong-Kook;Hwang, Sung-Joo
    • Journal of Pharmaceutical Investigation
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    • v.12 no.4
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    • pp.126-131
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    • 1982
  • A quantitative fluorometric method was developed to determine aspirin and salicylic acid in bulk aspirin and commercial aspirin tablets. The excitation maximum for aspirin was observed at 280 nm and the emission maximum was at 335nm. The lowest energy excitation band for salicylic acid was at 308nm and the fluorescence emission band was at 450nm. Excipients, binders, lubricants and impurities did not interfere. Excellent recoveries were obtained for aspirin and salicylic acid. Results obtained by the KP III procedure and the proposed method were compared.

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식물의 뿌리와 근권으로부터 Salicylic acid를 생성하는 근권세균의 검색 (Screening of Salicylic acid Producing Rhizobacteria Isolated from Plant Roots and Rhizosphere)

  • 이민웅
    • 한국식물병리학회지
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    • v.14 no.6
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    • pp.598-602
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    • 1998
  • Twenty two rhizobacteria were isolated from the roots and rhizosphere of radish, carnation, potato and tomato. There isolates produced a fluorescent pigment in King's B medium and identified as Pseudomonas spp. These isolates colonized roots and rhizosphere of the host plants. In the study of cultural characteristics of the bacteria, the pH of the culture broth was changed from neutral (7.0) to alkali (8.8∼9.41) and the numbers of cells were increased from 106 to 108 after 40 hr of incubation in basal standard succinate medium. The salicylic acid production identified by pink color reaction were observed in 7 bacteria. Out of these 7 salicylic acid producing bacteria, only 2 strains of bacteria such as Pseudomonas fluorescens RS006, and Pseudomonas sp. EN401 were confirmed as salicylic acid producers by optical density measurement. Therefore, for screening of salicylic acid producing bacteria from the roots and rhizosphere, color reaction of the culture medium should be done in the first step, and then optical density measurement of culture extract should be made for the confirmation of salicylic acid production.

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Production of Salicylic Acid from Naphthalene by Immobilized Pseudomonas sp. Strain NGK1

  • Shinde, Manohar;Kim, Chi-Kyung;Karegoudar, Timmanagouda-Baramanagouda
    • Journal of Microbiology and Biotechnology
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    • v.9 no.4
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    • pp.482-487
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    • 1999
  • The Pseudomonas sp. strain NGK1 (NCIM 5120) was immobilized in calcium alginate, agar, and polyacrylamide gel matrices. The salicylic acid-producing capacity of freely suspended cells was compared with immobilized cells in batches with a shake culture and continuous culture system in a packed bed reactor. Freely suspended cells ($4\times10^{10}cfu/ml$) produced 12 mM of salicylic acid, whereas cells immobilized in calcium alginate ($1.8\times10^{11}$cfu/g beads), agar ($1.8\times10^{11}$cfu/g beads), and polyacrylamide ($1.6\times10^{11}$cfu/g beads) produced 15, 11, and 16mM of salicylic acid, respectively, from naphthalene at an initial concentration of 25 mM. The continuous production of salicylic acid from naphthalene was investigated in a continuous packed bed reactor with two different cell populations. The longevity of the salicylic acid-producing activity of the immobilized cells from naphthalene was also studied in semi continuous fermentations. The immobilized cells could be reused 18, 13, and more than 20 times without losing salicylic acid-producing activity in calcium alginate-,agar-, and polyacrylamide-entrapped cells, respectively. The study reveals a more efficient utilization of naphthalene and salicylic acid production by the immobilized Pseudomonas sp. strain NGK1 as compared to the free cells.

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가시오갈피나무 불정근(不定根) 배양(培養)에서 불정근(不定根)의 생장(生長)과 Eleutheroside류(類)의 생산(生産)에 미치는 Salicylic Acid 처리(處理)의 영향 (Effect of Salicylic Acid on the Root Growth and the Eleutheroside Accumulation in the Adventitious Root Culture of Eleutherococcus senticosus)

  • 안진권;이위영;박응준
    • 한국산림과학회지
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    • v.100 no.2
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    • pp.178-183
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    • 2011
  • 생물반응기를 이용한 가시오갈피 부정근 배양시에 salicylic acid를 농도별(0, 5, 10, 20, 40, 80 ${\mu}M$)로 처리하여 부정근의 생장과 eleutheroside류 생산과의 관계를 조사하였다. Salicylic acid 처리농도별 부정근의 생장은 무처리구에서 4.4 g dry weight(DW)/L로 가장 높았으며, salicylic acid 농도가 증가할수록 부정근 생장은 감소하였다. 그러나 eleutheroside B, E 및 $E_1$의 함량은 대체로 salicylic acid 농도가 높을수록 증가하여 $80{\mu}M$ 처리구에서 각각 179.5, 1169.9 및 $45.4{\mu}g/g$ DW로 생산량이 가장 많았다. 배지 1 L당 eleutheroside류의 총생산량은 무처리구에서 $4975.8{\mu}g/L$를 생산하여 가장 우수하였다. $80{\mu}M$의 salicylic acid 처리 후 15일간 eleutheroside류의 함량을 조사한 결과 eleutheroside B는 salicylic acid처리 후 9일째, eleutheroside E 및 $E_1$은 처리 후 6일째 각각 가장 많은 생산량을 보여 주었다.

소나무류(類) 침엽내(針葉內)의 salicylic acid에 의한 솔잎혹파리의 방제효과(防除效果) (Effects of Controlling the Pine Needie Gall Midaes by Salicylic Acid Content in Needles of Some Pinus spp.)

  • 손두식;엄태진;최장옥;장약명
    • 한국산림과학회지
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    • v.88 no.1
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    • pp.31-37
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    • 1999
  • 소나무, 해송의 솔잎혹파리 충영형성율(蟲廮形成率)은 35~40%이나 버지니아소나무, 리기다소나무에는 산란(産卵)은 하나 충영(蟲廮)이 형성(形成)되지 않으므로 침엽내(針葉內)에 솔잎혹파리에 대한 저항성물질(抵抗性物質)이 존재(存在)함을 알 수 있었다. 솔잎혹파리의 감수성(感受性) 수종(樹種)인 소나무, 해송의 침엽내(針葉內) free phenol상태의 salicylic acid는 소량(小量) 함유(含有)되어 있으나 내충성(耐蟲性) 소나무인 버지니아소나무, 리기다소나무에는 37~50ppm이 함유(含有)되어 있어 salicylic acid는 솔잎혹파리에 대한 저항성물질(抵抗性物質)로 추정(推定)된다. 솔잎혹파리에 감수성(感受性) 수종(樹種)인 소나무와 해송에 salicylic acid의 용액(溶液)을 관수(灌水) 혹은 분말(粉末)을 뿌리에 주어 침엽내(針葉內) salicylic acid의 함량(含量)을 높여 줌으로써 무처리구(無處理區)에 비하여 충영형성율(蟲廮形成率)을 17~19배(倍)로 감소(減少)시킬 수 있었다. Salicylic acid에 의한 솔잎혹파리의 방제(防除)는 유충(幼蟲)이 알에서 부화(孵化)하기 전(前)에 처리(處理)하는 것이 효과적(效果的)이며, 해송과 버지니아소나무 교잡종(交雜種)의 충영형성율(蟲廮形成率)은 양친수종(兩親樹種)의 중단수치(中間數値)인 24%이나 교잡종중(交雜種中) 선발(選拔)한 개체(個體)에 따라 2.8%, 11.5%의 충영형성율(蟲廮形成率)을 나타내므로 침엽내(針葉內)salicylic acid의 함량(含量)에 의해서 솔잎혹파리에 대한 내충성(內蟲性) 개체(個体)를 선발(選拔)할 수 있을 것으로 기대된다.

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A Spectroscopic Study of Hydrogen Bonding between Riboflavin and Salicylic Acid Derivatives

  • Huh, Jae-Wook;Yu, Byung-Sul
    • 약학회지
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    • v.20 no.3
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    • pp.130-137
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    • 1976
  • Specific association phenomena of riboflayin-2',3',4',5',- tetraacetate and salicylic acid derivatives, such as salicylic acid, aspirin and salicylamide have been measured by infrared and fluorescence spectroscopy. Salicylic acid and riboflavin tetraacetate oxyl group of the former. Asprin and riboflavin tetraacetate form the 1:1 cyclic hydrogen bonded dimer by the same mode. Salicylamide froms the 1:1 cyclic hydrogen bonded dimer with riboflavin tetraacetate by using its amide group and carbonyl group. Salicylic acid derivatives are effective quenchers of the fluorescence of riboflavin tetraacetate. It is appeared that salifylamide is the strongest quencher among them. The quenching effect is attributed to the formation of association dimer.

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Alleviating Effect of Salicylic Acid Pre-treatment on Soil Moisture Stress of Waxy Corn

  • Seo, Youngho;Ryu, Sihwan;Park, Jongyeol;Choi, Jaekeun;Park, Kijin;Kim, Kyunghi
    • 한국토양비료학회지
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    • v.48 no.3
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    • pp.213-217
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    • 2015
  • Soil moisture shortage can reduce yield of waxy corn because maize is one of the sensitive crops to the drought stress. Farmers cannot irrigate due to limited water resource and irrigating facilities although applying water is the most effective practice to solve the drought problem. The study was conducted to investigate the pre-treatment effect of salicylic acid on reducing drought damage of waxy corn (Zea mays L.). Salicylic acid at concentration of 0.2 mM was applied at seven-leaf stage or ten-leaf stage three times. Drought stress was imposed by withholding irrigation from 11 days before anthesis to 10 days after anthesis. Application of salicylic acid significantly increased ear length by 11.0~12.3% and yield by 8.8~11.3% compared with non-treated control, indicating that the drought injuries of waxy corn can be alleviated through pre-treatment of salicylic acid at the vegetative stage.

Molecular interaction between a reduced riboflavin derivative and salicylic acid derivatives

  • Yu, Byung-Sul;Sohn, Dong-Hwan;Sohn, Dong-Hwan
    • Archives of Pharmacal Research
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    • v.8 no.3
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    • pp.99-107
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    • 1985
  • The interaction of reduced riboflavin 2', 3', 4', 5'-tetrabutyrate with salicylic acid, aspirin, and salicylamide has been spectroscopically investigated to determine the binding mechanism. Hydrogen-1 and carbon-13 unclear magnetic resonance, infrared, and absoption spectra were measured in chloform-d and chloroform. The association of the reduced riboflavin with salicylic acid derivatives is different from that osidizd one. Salicylic acid and the reduced riboflavin form a cyclic hydrogen bounded complex through the imino (3-N, 5-N) protons and the carbonyl (2-C, 4-C) oxygens of the isolloxazine ring of the latter, and the carboxylic hydroxyl proton and carbonyl oxygen of the former. Aspirin and the reduced riboflavin form a complex by the same mode as salicylic acid. Salicylamide forms a cyclic hydrogen bonded complex with the reduced riboflavin through the imino (3-N, 5-N) protons and the carbonyl (2-C, 4-C) oxygens of the isoalloxazine ring, and the amino proton and the carbonyl oxygen of salic aylmide. It appears that both the oxidized and reduced form of riboflavin are associated with salicylic acid derivatives.

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소나무류(類) 침엽내(針葉內)에 함유(含有)된 phenolic compounds 중 솔잎혹파리의 저항성(抵抗性) 물질(物質) 조사(調査) (A study on Resistant Substance to Pine Needle Gall Midge Among Phenolic Compounds in Pine Needles)

  • 손두식;엄태진;서재덕;이상록
    • 한국산림과학회지
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    • v.85 no.3
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    • pp.372-380
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    • 1996
  • 솔잎혹파리에 대한 저항성인자(抵抗性因子)를 조사(調査)하기 위하여 솔잎혹파리의 감수성수종(感受性樹種)과 저항성수종(抵抗性樹種)에 대하여 침엽내(針葉內) phenolic compound의 계절적(季節的) 변화(變化)를 조사(調査)하였다. 침엽내(針葉內)에 존재(存在)하는 free phenolic compounds와 water soluble phenolic compounds는 수종간(樹種間) 계절별(季節別)로 변이(變異)가 있었고 catechol과 vanilic acid 및 syringic acid는 수종간(樹種間)에는 큰 차이(差異)가 없으나 성분량(成分量)에는 계절별(季節別)로 변이(變異)가 있었다. 그러나 솔잎혹파리 유충(幼蟲)의 부화기(孵化期)인 5, 6월(月)에 침엽내(針葉內)에 함유(含有)되어 있는 salicylic acid는 버지니아소나무는 140ppm, 해송에서 35ppm, 해송${\times}$버지니아소나무 72ppm이고 소나무에서는 극소량만이 나타나고 있어 salicylic acid는 감수성수종(感受性樹種)에는 극소량 함유(含有)하고 저항성수종(抵抗性樹種)에는 상당량 함유(含有)하고 있었다. 솔잎혹파리에 대한 소나무류(類) 침엽(針葉)의 저항성인자(抵抗性因子) 조사(調査)에서 밝혀진바와 같이 salicylic acid와 버지니아소나무의 침엽(針葉)에서 추출(抽出)된 phenolic compounds용액(溶液)위에서 배양(培養)한 유충(幼蟲)은 폐사율(斃死率)이 높았고 또한 버지니아소나무에서는 충영이 전혀 형성되지 않은 것과 본조사(本調査)의 침엽내(針葉內)에 함유(含有)하고 있는 salicylic acid와 상관관계(相關關係)가 있는 것으로 사료된다. 이상의 결과(結果)에서 salicylic acid는 솔잎혹파리의 저항성물질(抵抗性物質)로 추정(推定)된다.

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