• Title, Summary, Keyword: selective media

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Application of selective MMSE scheme to the shifted TR for frequency selective channel (주파수 선택적 채널에서의 shifted TR 기반의 선택적 MMSE 기법 응용)

  • Cho, Jaewoong;Yoon, Misun;Lee, Bumsoo;Lee, Chungyong
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • pp.560-562
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    • 2011
  • 본 논문에서는 송신단에서의 shifted TR 기법과, 이 것에 수신단에서의 선택적 MMSE 등화기를 추가한 통신시스템을 주파수 선택적 채널에서 실험해보고 이것이 shifted TR 기법만 사용했을 때와 비교하여 수신단의 선택적 MMSE 등화기가 성능에 어떤 영향을 끼치는지 관찰하였다. 이를 보호 구간 별로 실험 결과를 비교하였으며 수신단에 선택적 MMSE 등화기를 추가시켰을 경우, shifted TR 기법만을 사용했을 경우보다 비트 오차율 측면에서 성능의 이득이 있음을 확인하였다.

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Genetic Recombination of Brevibacterium lactofermentum by Protoplast Fusion (Brevibacterium lactofermentum의 원형질체 융합에 의한 유전자 재조합)

  • 이혜경;최순영;윤윤경;이영하;민경희
    • Korean Journal of Microbiology
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    • v.28 no.2
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    • pp.98-103
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    • 1990
  • Brevibacterium lactofermentum SWA (arg trp) and B. lactofermentum SWB (met ser) were obtained from UV and NTG treatment. The rates of protoplast formation by B. lactofermentum SWA and SWB were 99.93% and 99.98%, respectively when each strain was treated with penicillin G in mid exponential growth phase, followed by incubation with 400 $\mu\textrm{g}$/ml of lysozyme in lysis fluid supplemented with 0.4M sucrose. Frequencies of protoplast regeneration in B. lactofermentum SWA and B. lactofermentum SWB were 9.27% and 10.32% respectively, on regeneration medium containing 0.5M sodium succinate, 50 mM $Mg^{2+}$, and 3% PVP. In intraspecific protoplast fusion between B. lactofermentum SWA and B. lactofermentum SWB, fusion frequency of $2.30\times 10^{-5}$ was observed by using the 100mM $CaCl_{2}$ and 30% PEG 6,000 in fusion fluid. Relative recombinant frequencies in each marker by means of selective media could be used for genetic analysis.

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Comparison of Culture, Conventional and Real-time PCR Methods for Listeria monocytogenes in Foods

  • Kim, Dong-Hyeon;Chon, Jung-Whan;Kim, Hyunsook;Kim, Hong-Seok;Choi, Dasom;Kim, Young-Ji;Yim, Jin-Hyeok;Moon, Jin-San;Seo, Kun-Ho
    • Food Science of Animal Resources
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    • v.34 no.5
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    • pp.665-673
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    • 2014
  • We compared standard culture methods as well as conventional PCR and real-time PCR for the detection of Listeria monocytogenes (L. monocytogenes) in milk, cheese, fresh-cut vegetables, and raw beef that have different levels of background microflora. No statistical differences were observed in sensitivity between the two selective media in all foods. In total, real-time PCR assay exhibited statistically excellent detection sensitivity (p<0.05) and was less time consuming and laborious as compared with standard culture methods. Conventional culture methods showed poor performance in detecting L. monocytogenes in food with high levels of background microflora, generating numerous false negative results. While the detection of L. monocytogenes in fresh cut vegetable by culture methods was hindered only by L. innocua, various background microflora, such as L. innocua, L. welshimeri, L. grayi, and Enterococcus faecalis appeared on the two selective media as presumptive positive colonies in raw beef indicating the necessity of improvement of current selective media. It appears that real-time PCR is an effective and sensitive presumptive screening tool for L. monocytogenes in various types of foods, especially foods samples with high levels of background microflora, thus complementing standard culture methodologies.

Proposed Method of Selective Broadcasting System for VR Personal Media Viewer (VR 개인방송 시청자를 위한 선택적 방송 시스템 제안)

  • Nam, Jin-Sol;Kim, Min-Ju;Kang, Ye-Jin;Park, Goo-Man
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • pp.161-163
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    • 2017
  • 다양한 연령층에서 시청자가 늘어나고 있는 개인방송에서도 VR 서비스가 확대되고 있다. 기존의 VR 방송은 360도 카메라로 촬영된 영상을 시청자들이 VR로 보거나 개인방송 크리에이터들이 HMD를 착용하고 콘텐츠를 플레이 하는 영상을 시청자들이 시청하도록 구성되어있다. 시청자들의 몰입감과 흥미를 더욱 유발하기 위해 본 논문에서는 시청자의 입장에서 크리에이터가 진행하는 방송도 시청할 수 있고, 그 콘텐츠를 직접 즐길 수 있는 선택적 방송 시스템을 제안한다. 제안된 시스템은 기존의 시스템보다 시청자로 하여금 몰임감을 향상시키고 참여 한계를 극복할 수 있다.

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Comparison of Real-Time PCR and Culture Methods for Detection of Campylobacter jejuni in Various Foods (다양한 식품에서 Campylobacter jejuni 검출을 위한 real-time PCR과 배지배양법의 비교검증)

  • Chon, Jung-Whan;Hyeon, Ji-Yeon;Hwang, In-Gyun;Kwak, Hyo-Sun;Han, Jeong-A;Kim, Moo-Sang;Kim, Jong-Hyun;Song, Kwang-Young;Seo, Kun-Ho
    • Korean Journal of Food Science and Technology
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    • v.43 no.1
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    • pp.119-123
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    • 2011
  • In this study, performances of culture methods using two selective media and real-time PCR were evaluated for detection of Campylobacter jejuni (C. jejuni) in various food samples. Sausage, ground beef, and radish sprouts inoculated with C. jejuni were enriched in Hunt broth and then streaked onto modified cefoperazone charcoal deoxycholate agar and Preston agar, followed by incubation under microaerobic conditions. The enriched Hunt broth (1 mL) was used in real-time PCR assay. No statistical differences were observed in sensitivity among the two selective media and real-time PCR for sausage and ground beef. However, the number of positives by real-time PCR in radish sprouts was much higher than the two selective media (p<0.05). It appears that real-time PCR could be used as an effective screening tool to detect C. jejuni, particularly in foods with a high number of background microflora such as fresh vegetables.

Spectrophotometric Determination of Nitrite Based on Its Reaction with p-Nitroaniline in the Presence of Diphenylamine in Micellar Media

  • Afkhami, Abbas;Masahi, Shokofeh;Bahram, Morteza
    • Bulletin of the Korean Chemical Society
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    • v.25 no.7
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    • pp.1009-1011
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    • 2004
  • In the present work a very simple, sensitive and selective spectrophotometric method for the determination of nitrite in micellar media is described. The method is based on the color reaction of nitrite with p-nitroaniline in the presence of diphenylamine in acid media. In order to remove the extraction step, Triton X-100, a non-ionic surfactant was used as micellar media. The optimum reaction conditions such as acid concentration, reagents concentration and effect of time have been studied and the analytical characteristics of the method such as limit of detection, linear range and molar absorptivity have been obtained. The interference of some anions and cations was also tested. The method was applied to the determination of nitrite in real samples.

Signaling Method for Spatial Adjacency Matrix of UWV media in MPEG Media Transport Environment (MPEG Media Transport 환경 내 UWV 미디어 공간 인접 행렬 시그널링 방안)

  • Kim, Junsik;Kang, Dongjin;Lee, Euisang;Kim, Kyuheon
    • Journal of Broadcast Engineering
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    • v.23 no.2
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    • pp.261-273
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    • 2018
  • As progress on image processing, computer vision and display technologies aroused market's interests on generation and consumption of various types of media, interests on UWV media are also increasing. In context of consumption of UWV media, to effectively manage load of servers and resources of end terminal devices and provide user-derived services, technology which enables users to select and consume interested regions of media seems to be needed. Here, this paper proposes a method for description and transmission of spatial relationships among media, which composes UWV, by expanding MPEG-CI and Layout signaling to enable users' selective consumption of UWV media.

Transmission Characteristics of FFH-SS Communication System in Fading Media (페이딩 매질에서 대역확산 주파수도약 통신시스템의 전송특성)

  • Kim, Won-Hu;Jeon, Gye-Seok
    • The Journal of the Acoustical Society of Korea
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    • v.6 no.1
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    • pp.5-14
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    • 1987
  • In this paper, the transmission characteristics of a frequency-hopped spread spectrum communication system operating in the presence of both time and frequency-selective fading channel is presented. The receiver is a binary noncoherent matched-filter FFH-SS system with square-law combiner. The probability of error to the variations of the parameters such as signal-to-noise ratio, selectivity of a channel, and power ratio is derived with the use of diversity concept without considering the data rate. The analysis of the data for probability of error shoves that the performance of FFH-SS system in time-selective fading channel is better than in frequency-selective fading channel.

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Stabilities of Plasmid Vectors in Zymomonas mobilis (Zymomonas mobilis Plasmid Vector의 숙주세포 내에서의 안정성에 관한 연구)

  • 이상기;박은숙;황덕주;박무영
    • Microbiology and Biotechnology Letters
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    • v.15 no.5
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    • pp.328-333
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    • 1987
  • The stabilities of plasmid vectors in Zymomonas mobilis were tested in batch and continuous cultures. It was found that the growth of the host Zymomonas strain was greatly affected by the size of plasmids as well as the composition of nutrient media: the host cells grew taster when harboring plasmids of smaller sizes and in n non-selective medium. All the Zymomonas plasmid vectors containing antibiotics selective markers and Zymomonas replication origins could be maintained in a stable manner over 30 generations without being integrated into host chromosomes.

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