• 제목/요약/키워드: selenium

검색결과 665건 처리시간 0.117초

성인 여성의 생애주기별 셀레늄 영양상태에 대한 평가 (Assessment of Selenium Status in Adult Females According to Life Cycle)

  • 이옥희;문종화;정용삼
    • Journal of Nutrition and Health
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    • v.36 no.5
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    • pp.491-499
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    • 2003
  • Selenium is a strong antioxidant trace mineral, scavenging free radicals. The prevalence of chronic degenerative diseases is increasing in Korean adults with increasing age. The increased cell damage from free radicals has been implicated in the etiology of these diseases, and evidence is accumulating that the low selenium status that comes with advanced aged is involved in the prevalence of age-associated diseases. However, little is known about the selenium status of Koreans, its age-related change and its relationship to dietary nutrient intake. In this study, the serum selenium levels of healthy adult females according to life cycle and its association with blood albumin levels and nutrient intake were examined. Serum selenium level was measured with the Huwo research reactor using the neutron activation analysis method (NAA). The overall proportion of women with selenium deficiency, serum selenium concentrations below 7.0 $\mu\textrm{g}$/dl, was 9.4%. The average serum selenium levels were 12.39 $\mu\textrm{g}$/dl, 9.45 $\mu\textrm{g}$/dl and 9.16 $\mu\textrm{g}$/dl in the young adult, middle-aged and elderly groups, respectively, showing a reduction of selenium status with advancing age. Selenium deficiency was seen only in the elderly group. Generally, serum selenium levels positively or negatively correlated with nutrient intake, but these association patterns differed depending on the age. The nutrients that showed positive correlations with selenium levels were proteins and phosphate in the young adult group (p < 0.05, p < 0.05), and total calcium, potassium and vegetable-origin calcium in the middle-aged group. Vitamin C and fiber were the negative correlated nutrients with serum selenium levels in the elderly group (p < 0.05, p < 0.05). Multiple stepwise regression analysis of the determining factors responsible for selenium status showed that age and serum albumin levels were important factors which explained up to 26.9% variances in serum selenium levels. The average selenium concentrations of Korean adult female subjects were above the deficiency levels in all three age groups. There was a tendency toward decreasing selenium levels as the age of the subjects increased. The factors with the strongest in-fluence on selenium status in healthy adult Korean females were age and serum protein status. (Korean J Nutrition 36(5): 491~499, 2003)

셀레늄 결핍식이를 먹인 쥐를 대상으로 유기셀레늄의 생체이용률에 대한 연구 (Bioavailability of Organic Selenium in Selenium-Deficient Rats)

  • 정은영
    • 한국식품영양과학회지
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    • v.44 no.9
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    • pp.1249-1255
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    • 2015
  • 본 연구는 세리신을 이용해 만든 유기셀레늄의 생체이용률을 알아보고자 하였다. 그 결과를 요약하면 다음과 같다. 항산화지표인 환원형 글루타티온은 셀레늄 처치로 인해 증가 되었는데 유기셀레늄에 의한 증가는 무기셀레늄에 비해 큰 경향이었으며, 또한 혈중 과산화 지질도 유기셀레늄이 무기셀레늄에 비해 낮은 값을 나타내어 유기셀레늄은 무기셀레늄에 비해 항산화력 향상에 더 기여하는 것으로 나타났다. 셀레늄 결핍식이로 인해 저하된 셀레늄 흡수율과 보유율은 셀레늄 처치로 증가되는데, 특히 유기셀레늄 처치로 인한 흡수율과 보유율이 높아 생체 내 이용률은 증대될 것으로 사료되며 이는 항산화력 향상에 영향을 미칠 것으로 사료된다. 혈청과 간의 셀레늄의 농도는 셀레늄 처치로 유의하게 증가되는데 셀레늄 형태 중 유기셀레늄에 의한 증가가 가장 컸으나 통계적으로 유의한 수준은 아니었다. 결론적으로 본 연구의 결과 무기질을 함유한 펩타이드는 무기질의 생체이용률을 증가시키는 것으로 나타났는데 세리신을 이용한 유기화 형태로 섭취될 경우 혈액과 장기의 침착뿐 아니라 흡수율과 보유율 증가에 관여하여 각 무기질의 효능을 증진시키는 것으로 나타났다.

Selenium의 첨가가 육계 및 산란계의 생산성 및 축적에 미치는 효과 (Effects of Dietary Selenium Sources on Performance and Selenium Retention in Broiler Chickens and Laying Hens)

  • 나재천;김상호;장병귀;김지혁;유동조;이덕수;이상진;이종찬;이원준
    • 한국가금학회지
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    • v.33 no.3
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    • pp.195-202
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    • 2006
  • 셀레늄의 첨가가 육계 및 산란계의 생산성과 축적에 미치는 영향을 구명하기 위하여 2차례의 실험을 수행하였다. 실험 1은 무기태 selenium 0.12, 0.24 ppm과 유기태 selenium 0.12, 0.24 및 0.6 ppm의 첨가가 육계의 사료 섭취량, 증체량, 사료 요구율 및 닭고기 내 selenium 축적에 미치는 영향을 조사하였다. 시험 전 기간의 증체량은 무기태 0.24 ppm 첨가구보다 유기태 selenium 0.24와 0.60 ppm 첨가구에서 높았다(P<0.05). 그러나 사료 섭취량과 사료 요구율은 대조구, 무기태 및 유기태 selenium의 첨가 수준에 의한 차이는 없었다. 가슴과 다리근육의 selenium 함량은 대조구에 비하여 유기태와 무기태 selenium 첨가구가 높았으며(P<0.05), 유기태 selenium의 첨가 수준에 따른 차이는 있으나(P<0.05), 무기태 selenium의 첨가 수준에 따른 차이는 없었다. 실험 2에서는 산란계 사료에 무기태 selenium 0.06, 0.12 ppm과 유기태 selenium 0.06, 0.12 및 0.30 ppm의 첨가하여 급여시에 산란율, 사료 섭취량, 사료 요구율, 난질 및 계란 내 selenium 함량에 미치는 영향을 조사하였다. 사료 요구율은 무기태 0.06 ppm 첨가구가 대조구보다 유의적으로 개선되었으나(P<0.05), 산란율, 난중, 1일 산란량, 사료 섭취량은 대조구, 무기태 및 유기태 selenium의 첨가 수준에 따른 차이는 없었다. Haugh unit는 모든 처리구에서 차이가 없었으며, 난황색은 유기태 selenium 0.30 ppm 첨가구가 대조구를 포함한 다른 처리구에 비하여 높았다(P<0.05). 난각 강도는 유기태 selenium 0.06 ppm 첨가구가 높았다.(P<0.05). 그러나 난각 두께는 대조구를 포함한 모든 처리구에서 차이는 없었다. 계란 중의 selenium 함량은 대조구에 비하여 selenium을 첨가한 모든 처리구에서 높았다(P<0.05). 이외에도 사료내 selenium 첨가 수준에 비례하여 계란에서 selenium 축적량도 증가하였으며(P<0.05), 특히 유기태 selenium 이 무기태 selenium보다 효과적이었다.

Production of Selenium Peptide by Autolysis of Saccharomyces cerevisiae

  • Lee Jung-Ok;Kim Young-Ok;Shin Dong-Hoon;Shin Jeong-Hyun;Kim Eun-Ki
    • Journal of Microbiology and Biotechnology
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    • v.16 no.7
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    • pp.1041-1046
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    • 2006
  • Selenium-containing peptide (selenium peptide) was produced by autolysis of total proteins of Saccharomyces cerevisiae grown with inorganic selenium. Selenium peptide exhibited antioxidant activity as a glutathione peroxidase (GPx) mimic, and its activity was dependent on the hydrolysis methods. The GPx-like activity of the hydrolyzed selenium peptide increased 2.7-folds when digested by protease, but decreased by acid hydrolysis. During the autolysis of the yeast cell, the GPx-like activity and selenium content increased 4.3- and 2.3-folds, respectively, whereas the average molecular weight (MW) of selenium peptide decreased 70%. The GPx-like activity was dependent on the MW of selenium peptide and was the highest (220 U/mg protein) at 9,500 dalton. The maximum GPx-like activity (28,600 U/g cell) was obtained by 48 h of autolysis of the cells, which were precultured with 20 ppm of selenate. Selenium peptide showed little toxicity, compared with highly toxic inorganic selenium. These results show the potential of selenium peptide as a nontoxic antioxidant that can be produced by simple autolysis of yeast cells.

철분의 과잉섭취시 셀레늄 섭취수준이 철분과잉 축적에 대한 예방효과에 관한 연구 (Preventive Effect of Selenium Supplementation on Iron Accumulation of Rats Fed Diets Containing High Levels of iron)

  • 전예숙
    • Journal of Nutrition and Health
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    • v.30 no.3
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    • pp.318-325
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    • 1997
  • The purpose of this study was to investigate the effect of selenium supplementation of iron accumulation of rats fed diets containing high levels or iron. Sixty male Sprague-Dawley weaning rats were fed with diets containing various levels of iron(adequate : 35ppm, 2-fold : 70ppm, 4-fold : 140ppm) and selenium(adequat : 0.05ppm and high : 0.05ppm) for 12 weeks. Feed intakes of 2-fold and 4-fold iron groups were higher than that of adequate iron group. There was no difference body weight gain across iron and selenium containing diet groups. Hemoglobin level was increasd with iron increment and decreased with selenium supplementation. Iron contents in serum and tissues were increased as iron intake was increased. Liver iron content was decreased with selenium supplementation. Selenium content in liver was decreased with iron increment and increased with selenium supplementation. In the case of iron balance, iron excretion through urine and feces was significantly increased as iron intake was increased. However, apparent absorbability and retention rate of iron were not significantly affected by dietary iron or selenium.

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체외 배양시 생쥐난자의 성숙과 생존에 미치는 Selenium의 영향 (Effect of Selenium on Oocyte Maturation and Viability in vitro in Mouse)

  • 최은진;홍순갑;김해권;윤용달;이준영
    • 한국발생생물학회지:발생과생식
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    • v.10 no.2
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    • pp.115-125
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    • 2006
  • 본 실험은 생쥐 난자의 성숙과 생존에 미치는 selenium의 영향을 알아보고자 수행하였다. 난자의 성숙은 현미경을 통해 관찰하였으며, 핵막 붕괴(germinal vesicle breakdown, GVBD)와 극체 형성(polar body formation, PB)은 체외 배양 시작 후 각각 2.5, 13시간에 확인하였다. 난자의 생존은 72 시간동안 체외 배양하면서 형태학적 차이로 정상 난자와 비정상 난자를 판별하였다. 또한 각 단계별로 수집된 난자의 glutathione(GSH) 함량은 spectrophotometer를 사용하여 glutathione assay로 측정하였다. 결과는 다음과 같다; 저농도의 selenium($0.005\;{\mu}g/mL{\sim}0.5\;{\mu}g/mL$)은 핵막 붕괴율과 극체 형성률을 증가시켰지만, 고농도의 selenium($5\;{\mu}g/mL$)은 감소시켰다. 저농도의 selenium은 극체 형성 시기 난자의 생존율을 증가시켰지만, 고농도의 selenium은 대조군과 별 차이가 없었다. 저농도의 selenium은 난자내 GSH 함량을 높게 유지시켰지만, 고농도의 selenium은 GSH 함량을 감소시켰다. 또한 극체 형성 시기 난자내 GSH 함량은 핵막 붕괴 시기 난자에 비해 높았다. 본 실험으로 볼 때, 저농도의 selenium은 대사과정에서 발생하는 oxidative stress에 의한 손상을 감소시킴으로써 난자의 질적 향상을 도우며, 성숙률을 증가시키는 것으로 사료된다. 또한 저농도의 selenium은 난자내 GSH 함량을 증가시켜 극체 형성 난자의 생존율을 증가시키는 것으로 사료된다.

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Maternal selenium-supplementation at various stages of periconception period: influence on murine blastocyst morphology and implantation status

  • Mamon, Mark Anthony C.;Ramos, Gliceria B.
    • Journal of Animal Science and Technology
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    • v.59 no.4
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    • pp.7.1-7.13
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    • 2017
  • Background: Selenium is one of the trace minerals whose deficiency is known to lead to complications of female reproduction. The identified gaps in researches regarding selenium and pregnancy include optimizing the dosage of selenium supplementation, timing of supplementation, finding the best form and type of selenium, and selenium administration combined with other antioxidants. Hence, this study was conceptualized to address one of the identified gaps, that is, to find out the best timing of selenium administration around the time of pregnancy. Specifically, this study aimed to assess the effects of maternal Selenium-supplementation, administered at various stages of periconception period, on murine blastocyst morphology, percent occurrence of good quality blastocysts, and implantation status. Methods: ICR female mice were randomly assigned into the unsupplemented group (Group I) receiving basal diet without selenium, and treatment groups given with $3.0{\mu}g$ selenium-supplement per day during pregestation only (Group II), pregestation-throughout-gestation (Group III) and gestation only (Group IV). Both blastocyst morphology and implantation status were assessed. Results: The morphometric measurements of blastocysts appeared to be unaffected by selenium-supplementation at different stages of periconception. Selenium-supplementation at pregestation only (Group II) and gestation only (Group IV) produced higher percent occurrence of good quality blastocysts and lower percent pre-implantation loss than Group III. Among all the treatment groups, Group III (Selenium-supplementation during pregestation-to-gestation) yielded the lowest quality blastocysts and highest percent pre-implantation loss. Conclusion: Maternal selenium-supplementation during pregestation and gestation stages of the periconception period yielded a high percent occurrence of good quality blastocysts and pre-implantation success.

철분과 셀레늄의 섭취수준이 흰쥐의 망간 이용에 미치는 영향 (Effects of Iron and Selenium Intakes on Utilization of Manganese in Rats)

  • 전예숙;최미경
    • 동아시아식생활학회지
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    • v.7 no.2
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    • pp.143-151
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    • 1997
  • The purpose of this study was to investigate the effect of iron and selenium intakes on utilization of manganese in rats fed adequate, 2-fold, 4-fold iron and adequate, high selenium for 6 weeks. There was no difference feed intake across iron and selenium containing diet groups. Body weight gain in 2-fold iron and high selenium group(MFeHSe) was significantly higher than those in other groups. Serum iron level was increased with iron increment, and liver iron content was decreased with selenium supplementation. Selenium and manganese contents in tissues were decreased with iron increment. In the case of manganese balance, manganese excretion through feces was significantly increased as iron intake was increased. However, retention and apparent absorption of manganese were not significantly affected by dietary iron. From these results, it could be suggested that the supplementations of iron and selenium affected the manganese utilization. Therefore, it must be considered interaction with various minerals in micro-nutrient supplementations.

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비소와 크롬에 의한 산화적 스트레스와 염색체 상해에 대한 셀레늄의 방어 효과 (An anti-clastogenic Role of Selenium in Arsenic- and Chromium-induced Oxidative Stress Causing Chromosomal Damages)

  • 기혜성;손은희;박영철;맹승희;정해원
    • 한국환경보건학회지
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    • v.23 no.4
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    • pp.9-15
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    • 1997
  • This experiment was carried out to examine the roles of selenium in arsenic- and chromium-induced oxidative stress, which results in chromosomal damage, such as sister chromatid exchange (SCE) and chromosomal aberration (CA). For this purpose, the frequency of CA and SCE related to the level of 0xidative stress were analyzed. Selenium decreased the frequency of CA induced by As. In order to evaluate the effect of selenium on clastogenic factors, media from As- and Cr-treated cells were ultrafiltered and added again to cells in the presence or absence of selenium. Selenium decreased the frequency of SCE by As and Cr. This observation indicates the possibility of presence of clastogenic factor. In addition, the clastogenic factor would be involed in oxidative stress since selenium decreased the level of oxidative stress. Thus, it is suggested that selenium may play a role as an anti-clastogenic effector by preventing the oxidative stress, thereby decreasing the frequency of Asand Cr-induced chromosomal damage.

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Selenium이 MPTP(1-methy-4-phenyl-1,2,3,6-tetrahydropyridine)에 의해 유도된 생쥐의 신경독성에 미치는 영향 (Effect of Selenium Yeast on MPTP (1-methyl-4-phenyl-propion-oxypiperidine)-Induced Neurotoxicity in Mice)

  • 김석환;이주연;김여정;강혜옥;이항우;최종원
    • 생명과학회지
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    • v.16 no.2
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    • pp.266-273
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    • 2006
  • MPTP에 의해 유도된 Parkinsonism에 대한 selenium의 보호효과와 그 보호작용에 대한 항산화적 해독기전을 조사하기 위하여 MPTP 10mg/kg을 6일간 주사하고 selenium (25, 50, 100 ${\mu}g/kg$)을 10일간 경구 투여하였으며 처음 6일간은 selenium와 MPTP를 병용 투여하였다. 실험동물을 마지막으로 selenium을 투여하고 24시간 후에 치사시켜 일반적인 독성과 항산화 방어능과 관련된 지표성분과 monoamine oxidase와 같은 신경생화학적인 지표성분들을 뇌조직에서 측정하였으며 그 결과 다음과 같은 결론을 얻었다. 우선 MPTP를 투여함에 따라 운동능력이 저하되던 것이 selenium을 투여함에 따라 운동능력이 증가되었으며, 이러한 결과의 기전은 selelnium을 투여함으로써 MPTP를 $MPP^+$로 대사시키는 MAO-B의 활성을 억제하였으며 $MPP^+$에 의해 유도된 신경독성에 대한 selenium의 보호 효과는 selenium을 투여함으로써 활성산소 해독계인 SOD, catalase, glutathione peroxidase의 활성을 증가시키기 때문인 것으로 사료된다.