• Title, Summary, Keyword: serovars

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Comparison of soluble antigens from Leptospira interrogans serovars by SDS-PAGE, Crossed Immunoelectrophoresis and Immunoblotting (SDS-PAGE, Crossed Immunoelectrophoresis 및 Immunoblotting을 이용한 Leptospira interrogans 혈청형간 항원 비교)

  • Baik, Yeong-ok;Mah, Jum-sool
    • Korean Journal of Veterinary Research
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    • v.32 no.2
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    • pp.195-205
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    • 1992
  • The soluble antigen profiles and antigenic specificities of Leptospira interrogans serovars icterhaemorrhagiae, canicola, pomona and hardjo were examined by SDS-polyacrylamide gel electrophoresis, crossed immunoeletrophoresis and immunoblotting. The profiles of protein, glycoprotein and fraction containing N-acetylglucosamine of 4 serovars were compared. The protein profiles of 4 serovars were very similar except the range of 14,400 to 30,000 daltons. Molecular weight of glycoprotein of L, pomona was lower than other serovars. L canicola showed extra N-acetylglucosamine bands having molecular weight of 82,000 and 90,000 daltons. In crossed immunoelectrophoresis, a close antigenic relationship was found between L icterohaemorrhagiae and L canicola. In immunoblottings conducted with soluble antigens and rabbit antisera of 4 serovars, Leptospira interrogans serovars possessed cross-reactive antigens and serovar-specific antigens. The molecular weights of serovar-specific antigens were 45,000, 82,000 and 90,000, 31,000 and 24,000 daltons in L icterohaemorrhagiae, L canicola, L pomona, and L hardjo, respectively.

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Biochemical characterization of Bacillus thuringiensis, 23 serovars (Biochemical thuringiensis, 23 serovars의 생화학적 특성)

  • Lee, Hyung-Hoan;Park, Mi-Yeoun;Lee, Chang-Woon
    • Microbiology and Biotechnology Letters
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    • v.14 no.2
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    • pp.205-208
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    • 1986
  • The 23 serovars of Bacillus thuringiensis strain were commonly gram-positive and motile, formed endotoxin crystals, produced acid and alkali in the KIA media, and acid from glucose, hydrolyzed starch, and reduced nitrate but did not produce H$_2$S, oxidase and indole, did not decompose lysine, ornithine, phenylalanine, malonate, lactose, dulcitol, adonitol, inositol, sorbitol, arabinose, raffinose, rhamnose, maltose, and xylose. Eighteen serovars were positive in the MR tests and 15 in the VP tests. Four serovars used citrate. Five serovars produced urease, 5 $CO_2$ from glucose, 2 DNase, and 15 lecithinase. Twelve serovars decomposed arginine, 11 did sucrose, 2 manitol, and 9 salicin Serovar tohokuensis did not hemolyze, but the others did.

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Serological investigation of Ureaplasma urealyticum in Korean preterm infants

  • Eun, Ho Seon;Lee, Soon Min;Park, Min Soo;Park, Kook In;Namgung, Ran;Lee, Chul
    • Clinical and Experimental Pediatrics
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    • v.56 no.11
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    • pp.477-481
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    • 2013
  • Purpose: Ureaplasma colonization is related with perinatal complications in preterm infants. Little is known about the difference in virulence among various Ureaplasma urealyticum serovars. The aim of this study was to determine U. urealyticum serovars of preterm infants in order to assess whether any of the serovars were associated with bronchopulmonary dysplasia (BPD). Methods: Three hundred forty-four preterm infants with a gestational age less than 34 weeks admitted to Gangnam Severance Hospital neonatal intensive care unit from July 2011 to December 2012 were included in this study. Tracheal and gastric aspirations were conducted on infants to confirm Ureaplasma colonization. Ureaplasma colonization was confirmed in 9% of infants, of these, serovars were determined by real-time polymerase chain reaction. Results: A total of 31 infants (gestational age, $29.3{\pm}3.1$ weeks; birth weight, $1,170{\pm}790g$) were U. urealyticum positive. The Ureaplasma positive group treated for more days with oxygen and ventilation than the negative group (P<0.05). Histologic chorioamnionitis and moderate to severe BPD were more frequent in the Ureaplasma positive group than in the negative group (P<0.05). U. urealyticum isolates were either found to be a mixture of multiple serovars (32%), serovar 9 alone or combined with other serovars (39%), serovar 11 (26%), 2 (13%), 8 (10%), 10 (13%), and 13 (25%). No individual serovars were significantly associated with moderate to severe BPD and chorioamnionitis. Conclusion: This is the first study to describe the distribution of U. urealyticum serovars from Korean preterm infants. Ureaplasma -colonized infants showed higher incidence of BPD and chorioamnionitis.

Serotype Distribution and Virulence Profile of Salmonella enterica Serovars Isolated from Food Animals and Humans in Lagos Nigeria

  • Abraham, Ajayi;Stella, Smith;Ibidunni, Bode-Sojobi;Coulibaly, Kalpy Julien;Funbi, Jolaiya Tolulope;Isaac, Adeleye Adeyemi
    • Microbiology and Biotechnology Letters
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    • v.47 no.2
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    • pp.310-316
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    • 2019
  • Distribution of Salmonella enterica serovars and their associated virulence determinants is wide-spread among food animals, which are continuously implicated in periodic salmonellosis outbreaks globally. The aim of this study was to determine and evaluate the diversity of five Salmonella serovar virulence genes (invA, pefA, cdtB, spvC and iroN) isolated from food animals and humans. Using standard microbiological techniques, Salmonella spp. were isolated from the feces of humans and three major food animals. Virulence determinants of the isolates were assayed using PCR. Clonal relatedness of the dominant serovar was determined via pulsed-field gel electrophoresis (PFGE) using the restriction enzyme, Xbal. Seventy one Salmonella spp. were isolated and serotyped into 44 serovars. Non-typhoidal Salmonella (NTS; 68) accounted for majority (95.8%) of the Salmonella serovars. Isolates from chicken (34) accounted for 47.9% of all isolates, out of which S. Budapest (14) was predominant (34.8%). However, the dominant S. Budapest serovars showed no genetic relatedness. The invA gene located on SPI-1 was detected in all isolates. Furthermore, 94% of the isolates from sheep harbored the spvC genes. The iroN gene was present in 50%, 100%, 88%, and 91% of isolates from human, chicken, sheep, and cattle, respectively. The pefA gene was detected in 18 isolates from chicken and a single isolate from sheep. Notably, having diverse Salmonella serovars containing plasmid encoded virulence genes circulating the food chain is of public health significance; hence, surveillance is required.

Listeria monocytogenes Serovar 4a is a Possible Evolutionary Intermediate Between L. monocytogenes Serovars 1/2a and 4b and L. innocua

  • Chen, Jianshun;Jiang, Lingli;Chen, Xueyan;Luo, Xiaokai;Chen, Yang;Yu, Ying;Tian, Guoming;Liu, Dongyou;Fang, Weihuan
    • Journal of Microbiology and Biotechnology
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    • v.19 no.3
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    • pp.238-249
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    • 2009
  • The genus Listeria consists of six closely related species and forms three phylogenetic groups: L. monocytogenes-L. innocua, L. ivanovii-L. seeligeri-L. welshimeri, and L. grayi. In this report, we attempted to examine the evolutionary relationship in the L. monocytogenes-L. innocua group by probing the nucleotide sequences of 23S rRNA and 16S rRNA, and the gene clusters lmo0029-lmo0042, ascB-dapE, rplS-infC, and prs-ldh in L. monocytogenes serovars 1/2a, 4a, and 4b, and L. innocua. Additionally, we assessed the status of L. monocytogenes-specific inlA and inlB genes and 10 L. innocua-specific genes in these species/serovars, together with phenotypic characterization by using in vivo and in vitro procedures. The results indicate that L. monocytogenes serovar 4a strains are genetically similar to L. innocua in the lmo0035-lmo0042, ascB-dapE, and rplS-infC regions and also possess L. innocua-specific genes lin0372 and lin1073. Furthermore, both L. monocytogenes serovar 4a and L. innocua exhibit impaired intercellular spread ability and negligible pathogenicity in mouse model. On the other hand, despite resembling L. monocytogenes serovars 1/2a and 4b in having a nearly identical virulence gene cluster, and inlA and inlB genes, these serovar 4a strains differ from serovars 1/2a and 4b by harboring notably altered actA and plcB genes, displaying strong phospholipase activity and subdued in vivo and in vitro virulence. Thus, by possessing many genes common to L. monocytogenes serovars 1/2a and 4b, and sharing many similar gene deletions with L. innocua, L. monocytogenes serovar 4a represents a possible evolutionary intermediate between L. monocytogenes serovars 1/2a and 4b and L. innocua.

$Ureaplasma$ infections in pre-term infants: Recent information regarding the role of $Ureaplasma$ species as neonatal pathogens

  • Sung, Tae-Jung
    • Clinical and Experimental Pediatrics
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    • v.53 no.12
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    • pp.989-993
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    • 2010
  • Although numerous clinical observational studies have been conducted over a period of over 30 years, the clinical significance of $Ureaplasma$ infection is still under debate. The $Ureaplasma$ speices. is a commensal in the female genital tract and considered to have of low virulence; however, $Ureaplasma$ colonization has been associated with infertility, stillbirth, preterm delivery, histologic chorioamnionitis, and neonatal morbidities, including congenital pneumonia, meningitis, bronchopulmonary dysplasia, and perinatal death. Recently, $Ureaplasma$ was subdivided into 2 separate species and 14 serovars. $Ureaplasma$ $parvum$ is known as biovar 1 and contains serovars 1, 3, 6, and 14, and $Ureaplasma$ $urealyticum$ (biovar 2) contains the remaining serovars (2, 4, 5, and 7-13). The existence of differences in pathogenicities of these 14 serovars and 2 biovars is controversial. Although macrolides are the only antimicrobial agents currently available for use in neonatal ureaplasmal infections, in the current clinical field, it is difficult to make decisions regarding which antibiotics should be used. Future investigations involving large, multicenter, randomized, controlled studies are needed before proper recommendations can be made for clinical practice.

Comparison of two diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR to discriminate between Salmonella enterica serovars Gallinarum and S Pullorum (Salmonella enterica serovars Gallinarum과 S Pullorum의 감별을 위한 2가지 진단법: allele-specific real-time PCR과 3'-tailed PCR의 비교)

  • Lee, Se-Mi;Seo, Ja-Young;Lee, Jae-Il;Kim, Tae-Jung
    • Korean Journal of Veterinary Service
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    • v.31 no.4
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    • pp.485-492
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    • 2008
  • Salmonella enterica serovars Gallinarum(SG, causative agent of fowl typhoid) and S Pullorum(SP, causative agent of pullorum disease) are very important bacterial pathogens in poultry industry. They share some common antigenic properties though the characteristics of outbreaks are quite different. To discriminate between SG and SP, we developed two rapid diagnostic methods, allele-specific real-time PCR and 3'-tailed PCR over 2 single nucleotide polymorphisms ($237^{th}\;and\;598^{th}$). In both methods, $237^{th}$ allele was found to be a good target for differential diagnosis, while $598^{th}$ allele produced some non-specific reactions.

Serological survey on the leptospiral antibody in domestic animals in the area occurred human leptospirosis (사람 ?토스피라병 발생지역의 동물에 대한 ?토스피라균의 혈중 항체조사)

  • Seo, Ik-soo
    • Korean Journal of Veterinary Research
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    • v.31 no.2
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    • pp.209-215
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    • 1991
  • Serological survey were conducted on the leptospiral antibody in domestic animals which were fed in the three rural village occurred human leptospirosis. Names of three villages are Shinnam-li, Shinjeop-li and Jinai-li which are located in near the northeastern part of Yeoju town in Kyunggi province. Total 66 serum samples were collected from the domestic animals in which 12 dairy cows, 10 Korean native cattle, 12 pigs and 32 dogs were included. Leptospiral antibody were detected with 4 different serovars of leptospira living antigens, such as Leptospira icterohaemorrhagiae, L. pomona, L. canicola and L. tarassovi by microscopic agglutination test for each serum sample. The results are obtained as follow. 1. All 66 sera collected from the domestic animals at three villages showed negative reaction with 4 different serovars of leptospiral antigen. 2. Only one serum sample taken from a dairy cow in Shinjeop-li showed a weak positive reaction with Leptospira tarassovi. It is suggest that this positive case is not infected with L. tarassovi, but with vaccination. 3. It is indicated that all domestic animals which wen, fed in the villages occured human leptospirosis were not infected with above 4 different serovars of leptospira at least.

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Prevalence and antimicrobial resistance patterns of Salmonella spp. isolated from different aged pigs in Korea

  • Kim, Eun Mi;Kim, Hye Kwon;Park, Seong Jun;Lee, Chul Seung;Luo, Yuzi;Moon, Hyoung Joon;Yang, Jeong Sun;Park, Bong Kyun
    • Korean Journal of Veterinary Research
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    • v.47 no.4
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    • pp.395-398
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    • 2007
  • A total of 114 Salmonella spp. (17.90%) were isolated from 637 fecal swab samples collected in October 2005 and they were from 25 (78.13%) of 32 farms investigated nation-widely in Korea. Salmonella spp. from age group of 30-, 60-, 90-, 120-day olds were 17.61%, 16.98%, 15.72%, and 21.25%, respectively. Nine serovars of Salmonella spp. were identified. The predominant serovars of isolates were S. Typhimurium (including var Copenhagen), S. Agona, S. Derby, and S. Heidelberg by turns. Almost isolates belonged to serogroup B (69.30%). All isolates were resistant to penicillin G and oxytetracycline, and this was considered due to be used as feed additive through the most of pig farms for decades. And also, frequent resistance observed for ampicillin, sulfamethoxazole-trimethoprim, and lincomycin-spectinomycin, which are commonly used in veterinary medicine for decades, indicates an urgent need to utilize these antimicrobials more prudently if their benefits are to be preserved.

Study on V. parahaemolyticus Isolated from Diarrheal Patients in Japan and Indonesia (일본(日本) 및 인도네시아의 설사환자(患者)에서 분리(分離)한 장염(腸炎) vibrio에 관(關)한 연구(硏究))

  • Ju, Jin-Woo
    • The Journal of the Korean Society for Microbiology
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    • v.13 no.1
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    • pp.37-42
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    • 1978
  • Identification and serotyping for 21 strains of V. parahaemolyticus isolated from diarrheal patients in Japan and Indonesia in 1977 were carried out. The results were as follows: 1. The 21 strains were divided into 5 serovars including O1:K56, O4:K4, O4:K8, O4:K12 and O5:K15. 2. Author's experiences on the isolates in Korea also show that there is no particular difference in the distribution pattern of serovars of V. parahaemolyticus among Korea, Japan and Indonesia. In these respects, there was no geographical difference in these countries.

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